Abstract:
:NIH3T3 cells transformed by mouse Int-2/Fgf-3 cDNA express a series of Int-2-related products representing discrete stages of processing and glycosylation. We confirm that in at least two highly transformed clonal lines, Int-2 products acquire further modifications and are efficiently secreted into the culture medium. Secreted proteins become associated with the cell surface and extracellular matrix and can be displaced by addition of soluble glycosaminoglycans, specifically heparin, heparan sulfate, and dermatan sulfate. Increasing concentrations of heparin not only compete for Int-2 binding in a dose-dependent manner but also inhibit the growth of these cells and revert the transformed phenotype. These findings reaffirm the notion that extracellular or surface-bound Int-2 protein is instrumental in the morphological transformation of these cells.
journal_name
Mol Cell Bioljournal_title
Molecular and cellular biologyauthors
Kiefer P,Peters G,Dickson Cdoi
10.1128/mcb.11.12.5929subject
Has Abstractpub_date
1991-12-01 00:00:00pages
5929-36issue
12eissn
0270-7306issn
1098-5549journal_volume
11pub_type
杂志文章abstract::Using a hybridoma cell line which secretes hapten-specific immunoglobulin M (IgM), we have isolated a variety of mutants which produce abnormal immunoglobulin. Immunoglobulin was tested for the size and composition of the component heavy and light chains and for variable and constant region related functional and sero...
journal_title:Molecular and cellular biology
pub_type: 杂志文章
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journal_title:Molecular and cellular biology
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pub_type: 杂志文章
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pub_type: 杂志文章
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更新日期:1992-05-01 00:00:00
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更新日期:1987-06-01 00:00:00
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更新日期:2009-09-01 00:00:00
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pub_type: 杂志文章
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pub_type: 杂志文章
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