Abstract：
:Mouse embryonic stem (ES) cells were transplanted into the cochlea of adult guinea pigs in order to explore their survival, differentiation, and possible integration with the host tissue. With the purpose of investigating the possible effect of manipulating the local embryonic microenvironment, ES cells were transplanted into the cochlea with or without an embryonic neuronal cograft consisting of dorsal root ganglion (DRG) tissue. To detect the survival and differentiation of ES cells, cells expressing green fluorescent protein (GFP) were used in combination with immunohistochemical detection of a neuronal marker, neural class III beta-tubulin (TUJ1 antibody). At 4 weeks following transplantation implanted ES cells were found close both to the sensory epithelium, and the spiral ganglion neurons (SGNs) with their peripheral dendritic processes projecting to the organ of Corti. There was a significant difference in the number of surviving TUJ1 (+) ES cells between the DRG cograft group and the non-cograft group (P < 0.01, ANOVA). Neurite-like projections were also identified between TUJ1-positive ES cells and the peripheral dendritic processes from SGNs. The results suggest that an embryonic neuronal microenvironment may be one of the key factors in the survival and differentiation of ES cells in the adult auditory system.
journal_name
Brain Resjournal_title
Brain researchauthors
Hu Z,Andäng M,Ni D,Ulfendahl Mdoi
10.1016/j.brainres.2005.06.016subject
Has Abstractpub_date
2005-07-27 00:00:00pages
137-44issue
1-2eissn
0006-8993issn
1872-6240pii
S0006-8993(05)00857-7journal_volume
1051pub_type
杂志文章相关文献
BRAIN RESEARCH文献大全abstract：:The effects of intraspinal microinjectins of morphine (10 microgram) and methionine-enkephalin (Met-enkephalin) (5 microgram) on the C-fiber and polysynaptic reflexes in the acute decerebrate low spinal cat were investigated. Microinjected into the dorsal horn, morphine and Met-enkephalin depressed the nociceptive C-f...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(80)90409-6
更新日期：1980-09-08 00:00:00
abstract：OBJECTIVES:Neuroprotective benefits of ethanol (EtOH) and normobaric oxygenation (NBO) were previously demonstrated in transient and permanent ischemic stroke. Here we sought to identify whether the enhanced lactic acidosis and increased expression of monocarboxylate transporters (MCTs) observed after stroke might be a...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/j.brainres.2015.01.040
更新日期：2015-04-07 00:00:00
abstract：:Close to 20% of the patients infected with the AIDS virus develops neurological deficit; eventhough HIV does not invade neurons. Consistently with the neurological deficit, HIV(+) subjects show abnormalities in brainstem auditory and visual evoked potentials (BSAEP and VEP) and in sleep patterns. The HIV-derived glyco...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/s0006-8993(99)01572-3
更新日期：1999-07-31 00:00:00
abstract：:Noradrenergic and dopaminergic regulation of thyrotropin (TSH) secretion was investigated in adult male Wistar rats. TSH secretion displayed a circadian variation with peak serum TSH levels at 10.00 h. The alpha 2-adrenoceptor agonist, clonidine (250 micrograms/kg, i.p.), was found to cause an enhancement of serum TSH...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(87)91378-3
更新日期：1987-02-24 00:00:00
abstract：:The non-cholinergic excitatory potential elicited in neurons of the isolated inferior mesenteric ganglia was depressed by bath application of Met- or Leu-enkephalin and enhanced by naloxone or naltrexone. The membrane depolarization induced by exogenously applied putative transmitter substance P was not appreciably af...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(82)90211-6
更新日期：1982-03-04 00:00:00
abstract：:The effect of valproic acid (VPA) on synaptic transmission was investigated at the frog neuromuscular junction using electrophysiological methods. The drug caused a dose-dependent decrease in the quantal content of the endplate potential (EPP) and a significant reduction in the input resistance of the postjunctional m...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(84)90393-7
更新日期：1984-07-23 00:00:00
abstract：:The multidrug resistance gene (Abcb1) protein product, phosphoglycoprotein (P-gp) is expressed on the luminal surface of capillary endothelial cells of the adult blood-brain barrier (bbb). P-gp is critical for neuroprotection as it actively pumps substrates back into the capillary lumen. The fetal brain represents a p...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/j.brainres.2010.08.016
更新日期：2010-10-21 00:00:00
abstract：:Nerve fibers and terminals immunoreactive for cholecystokinin (CCK) were demonstrated in the nucleus of the solitary tract (NTS) of the rat using light and electron microscopic immunocytochemistry. The following morphological and biochemical evidence suggests that CCK in the NTS seems to be of extrinsic, most probably...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(82)90408-5
更新日期：1982-12-09 00:00:00
abstract：:Previous studies have observed that pial artery dilation to activators of the ATP sensitive K (K(ATP)) and calcium sensitive K (K(ca)) channel was blunted following fluid percussion brain injury (FPI) in the piglet. In recent studies in the rat, protein tyrosine kinase (PTK) activation was observed to contribute to K(...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/s0006-8993(02)02703-8
更新日期：2002-07-12 00:00:00
abstract：:The amygdala plays an important role in the regulation of motivational states, especially those associated with addiction. The amygdala also expresses high levels of brain-derived neurotrophic factor (BDNF), an activity-dependent neurotrophin that can influence the reinforcing and locomotor activating properties of ps...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/s0006-8993(02)03160-8
更新日期：2002-09-13 00:00:00
abstract：:1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) causes a disruption of nigrostriatal dopaminergic function which resembles parkinsonism. On the cellular level, the disruption involves a Ca2+ independent release of dopamine, depletion of nigral and striatal dopamine, and the death of dopaminergic neurons. We have p...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(91)91035-y
更新日期：1991-02-15 00:00:00
abstract：:The herpes virus suis has been used as a tracer for the pathways in the central nervous system of the rat. The viruses have been inoculated in various peripheral structures innervated by the trigeminal nerve, namely in the cornea by instillation or scarification, in the anterior chamber of the eye by injection and als...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(83)90850-8
更新日期：1983-08-29 00:00:00
abstract：:Aromatization of testosterone in the preoptic area of the male ring dove is required for the expression of specific estrogen-sensitive sexual behavior. Estradiol metabolism and/or binding of the aromatization product(s) to the preoptic estrogen receptors (ER) could interfere with the apparent increase in estradiol for...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(89)90203-5
更新日期：1989-02-20 00:00:00
abstract：:The basal ganglia of rhesus and squirrel monkeys were examined using immunohistochemical techniques with antibodies against the neurotensin-related hexapeptides Lys8-Asn9-Neurotensin(8-13) (LANT6) and Neuromedin N. A high percentage of neurons in both segments of globus pallidus and many large neurons of the striatum ...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(87)90557-9
更新日期：1987-09-29 00:00:00
abstract：:In anaesthetized cats, medial gastrocnemius motor units (MUs) were stimulated with random sequences (mean rates between 6 and 12 pps) of electrical pulses delivered to their axons in small ventral root filaments. Muscle tension was recorded under isometric conditions, and spike trains of muscle spindle afferents were ...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(86)91656-2
更新日期：1986-02-19 00:00:00
abstract：:Brain-derived neurotrophic factor (BDNF) is a family of neurotrophins that plays crucial roles in neural development, survival, maintenance and regeneration both in central and peripheral nervous systems. To examine the effects of BDNF on mastication, jaw movement trajectories and masticatory muscle activities were el...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/j.brainres.2004.04.061
更新日期：2004-07-30 00:00:00
abstract：:The conduction velocity of frog ischiadic nerves incubated in vitro in osmolarities between 220 and 1000 mOsm decreased with the degree of fiber shrinkage. The latter (non-circularity factor) was determined from computer-assisted measurements in freeze-substituted or in chemically fixed fibers. Freeze-substituted norm...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(84)90546-8
更新日期：1984-04-09 00:00:00
abstract：:SBA and NADPH-d histochemistries allow identification of functionally distinct components of the amphibian primary olfactory system. In Pipa, a secondarily aquatic frog, combination of both methodologies, using alternate sets of histological sections, reveals that, apart from Jacobson's organ, this species has a "wate...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/s0006-8993(97)00507-6
更新日期：1997-07-11 00:00:00
abstract：:The effect of different steady-state cocaine concentration maintenance on the microdialysis extraction fraction of cocaine (in situ recovery; Ed) in the medial prefrontal cortex (mPFC) was examined. Male, adult Sprague-Dawley rats anesthetized with chloral hydrate were used. All rats were randomly divided into three g...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(95)00456-z
更新日期：1995-07-24 00:00:00
abstract：:The vascular changes in the hippocampus and neocortex during kainic acid-induced seizures were investigated in control rats and in rats with chronic, bilateral, cervical sympathectomy. Seizures were induced in unanesthetized, spontaneously breathing rats. The increase in blood flow in the hippocampus of the sympathect...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(90)91782-c
更新日期：1990-11-05 00:00:00
abstract：:Glia represents the most numerous group of nervous system cells and CNS development and function depend on glial cells. We developed a purified Muller glia culture to investigate the expression of several neurotransmitter markers on these cells, such as dopaminergic, cholinergic, GABAergic and peptidergic receptors or...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/j.brainres.2005.01.031
更新日期：2005-03-21 00:00:00
abstract：:The effects of kindling induced in developing rat pups were re-examined when the rats reached maturity. Eighteen-day-old rats, implanted with bipolar electrodes, were separated into the following 4 groups: the fully kindled group consisted of pups that developed generalized convulsions; the partially kindled group of ...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0165-3806(82)90097-9
更新日期：1982-05-01 00:00:00
abstract：:The effect of an 8% casein and a control 25% casein diet on the granule cells in the dorsal blade of the dentate gyrus of the rat hippocampal formation was studied at 30, 90 and 220 days of age. Female rats were fed either an 8% or 25% casein diet 5 weeks prior to conception and the litters were maintained on these re...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(90)91769-d
更新日期：1990-11-05 00:00:00
abstract：:Dysfunction in the hypothalamic GABAergic system has been implicated in panic syndrome in humans. Furthermore, several studies have implicated the hypothalamus in the elaboration of pain modulation. Panic-prone states are able to be experimentally induced in laboratory animals to study this phenomenon. The aim of the ...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/j.brainres.2009.09.096
更新日期：2009-12-11 00:00:00
abstract：:Trophic effects of neurturin, a member of the glial cell line-derived neurotrophic factor-family, have been demonstrated on mesencephalic dopaminergic neurons, suggesting its therapeutic potential for Parkinson's disease. This study was designed to test the neuroprotective and regenerative effects of an intrastriatal ...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/s0006-8993(02)02934-7
更新日期：2002-08-30 00:00:00
abstract：:Results from clinical studies provide evidence that cognitive changes relatively late in life may be traced to antecedent conditions including diabetes, obesity, a sedentary lifestyle, and an atherogenic diet. As such, several traits of Type 2 diabetes (T2DM) could be considered pathogenic factors of aging, contributi...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/j.brainres.2014.10.052
更新日期：2015-09-24 00:00:00
abstract：:The inhibitory potency of ethanol upon excitatory amino acid induced depolarizations of rat hippocampal CA1 pyramidal cells was assessed in the presence and absence of magnesium (Mg2+) using the grease-gap technique. Ethanol shifted the N-methyl-D-aspartate (NMDA) dose-response curves to the right in a non-parallel ma...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(91)91486-k
更新日期：1991-04-19 00:00:00
abstract：:Data reported up to now on neuron-glia relationships, show that neuron cell bodies and glial cells are separated by a narrow intercellular cleft which is considered as the microenvironment of the nervous system, and where neuron-glia exchange occurs. We present here evidence that neuron perikarya and ensheathing glial...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(85)91394-0
更新日期：1985-02-04 00:00:00
abstract：:The effect of a single train of electrical hippocampal stimulation on ACTH and corticosterone (CS) responses to subsequent photic stimulation was studied in freely moving male rats. The hippocampal stimulation inhibited the stress-induced rise [corrected] in serum CS levels up to 150 h when compared to sham stimulated...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/s0006-8993(01)02538-0
更新日期：2001-08-17 00:00:00
abstract：:Ongoing development of the dopamine system during adolescence may provide a partial mechanism for behavioral and psychiatric vulnerabilities. Despite early evidence for a hyperactive adolescent dopaminergic system, recent data suggest that adolescent dopamine may be functionally hypoactive compared to in adults. While...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/j.brainres.2016.07.027
更新日期：2017-01-01 00:00:00
