Abstract:
:Sperm are motile cells. Thus, a significant component of the spermatogenic cycle is devoted to the formation of flagellum, a process that must be coordinated to insure proper construction. To document the temporal pattern of flagellar gene expression, we employed real-time PCR to assess changes in accumulation of a cohort of genes encoding axoneme, outer dense fibre (ODF) and fibrous sheath (FS) proteins during the first wave of spermatogenesis in the mouse. Axoneme genes were expressed first at the pachytene spermatocyte stage, followed by expression of transcripts encoding ODF and FS components. However, there were differences among these families with respect to the time of initial expression and the rate of mRNA accumulation. To gain understanding of factors that determine these patterns of expression, we cloned the promoters of three axoneme central apparatus genes (Pf6, Spag6 and Pf20). These promoters shared common features including the absence of a TATA box, and putative binding sites for several factors implicated in spermatogenesis (CREB/CREM, SOX17 and SPZ1) as well as ciliogenesis (FOXJ1). Collectively, our findings demonstrate a sequential pattern of expression of flagellar component genes, differential times of expression or rates of transcript accumulation within each class and shared promoter features within a class.
journal_name
Mol Hum Reprodjournal_title
Molecular human reproductionauthors
Horowitz E,Zhang Z,Jones BH,Moss SB,Ho C,Wood JR,Wang X,Sammel MD,Strauss JF 3rddoi
10.1093/molehr/gah163subject
Has Abstractpub_date
2005-04-01 00:00:00pages
307-17issue
4eissn
1360-9947issn
1460-2407pii
11/4/307journal_volume
11pub_type
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