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A chromism-based assay (CHROBA) technique for in situ detection of protein kinase activity.

Abstract：

:A unique chromism-based assay technique (CHROBA) using photochromic spiropyran-containing peptides has been firstly established for detection of protein kinase A-catalyzed phosphorylation. The alternative method has advantages that avoid isolation and/or immobilization of kinase substrates to remove excess reagents including nonreactive isotope-labeled ATP or fluorescently-labeled anti-phosphoamino acid antibodies from the reaction mixture. Such a novel protocol based on thermocoloration of the spiropyran moiety in the peptide can offer not only an efficient screening method of potent kinase substrates but also a versatile analytical tool for monitoring other post-translational modification activities.

journal_name

Bioorg Med Chem Lett

journal_title

Bioorganic & medicinal chemistry letters

authors

Tomizaki KY,Jie X,Mihara H

doi

10.1016/j.bmcl.2005.01.035

subject

Has Abstract

pub_date

2005-03-15 00:00:00

pages

1731-5

issue

6

eissn

0960-894X

issn

1464-3405

pii

S0960-894X(05)00082-X

journal_volume

15

pub_type

杂志文章

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