T-cell flow-cytometry crossmatch and long-term renal graft survival.

Abstract:

:Flow cytometry crossmatch (FCXM) is a more sensitive technique than classical complement-dependent cytotoxicity (CDC) for the detection of donor-directed antibody before renal transplantation. Nevertheless, the role of FCXM in predicting long-term survival of kidney grafts is still unclear. The purpose of our study was to evaluate the impact of a positive T-cell FCXM (T-FCXM) on long-term kidney allografts outcome. Of the 184 consecutive kidney transplantations performed in our center between 1 January1991 and 15 November 1996 a FCXM, performed concurrently to the pre-transplant CDCXM, was available for 170 patients. The CDCXM was negative in all recipients. Among these recipients, 12 (7.1%) had a positive T-FCXM. These patients were not different from patients with a negative T-FCXM for donor and recipient age, sex, frequency of second transplantation, number of human leukocyte antigen matches or mismatches. Frequency of immunized patients was higher in kidney recipients with a positive FCXM (58.3% vs. 24.7%; p=0.02, chi-square test). Survival analysis revealed that kidney graft outcome was better in negative T-FCXM recipients (p=0.03), while patient survival was not statistically different. Our results suggest that a positive pre-transplant T-FCXM despite a negative CDCXM is associated with an impaired long-term graft survival in renal allotransplantation.

journal_name

Clin Transplant

journal_title

Clinical transplantation

authors

Rebibou JM,Bittencourt MC,Saint-Hillier Y,Chabod J,Dupont I,Bittard H,Chalopin JM,Hervé P,Tiberghien P

doi

10.1111/j.1399-0012.2004.00226.x

subject

Has Abstract

pub_date

2004-10-01 00:00:00

pages

558-63

issue

5

eissn

0902-0063

issn

1399-0012

pii

CTR226

journal_volume

18

pub_type

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