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Expression of fungal pectin methylesterase in transgenic tobacco leads to alteration in cell wall metabolism and a dwarf phenotype.

Abstract：

:A transgenic tobacco plant (Nicotiana tabacum L.) expressing a fungal pectin methylesterase (PME; EC 3.1.1.11) gene derived from a black filamentous fungus, Aspergillus niger was created. Fungal PME should have a wider range of adaptability to substrate pectin compared with plant PME. As expected, the proportion of methyl esters in pectin was reduced in the transgenic tobacco. Consequently, the transgenic plant showed short internodes, small leaves and a dwarf phenotype. At a cellular level, the longitudinal lengths of stem epidermal cells were shorter than those of control plants. This is the first report that fungal PME promotes dwarfism in plants. It is worth noting that in the PME-expressing dwarf plant, the expression levels of cell wall metabolism related genes that included endo-1,4-beta-glucanase, cellulose synthase, endo-xyloglucan transferase and expansin gene were decreased. These results suggest that the expression of fungal PME in plants affects the cell wall metabolism.

journal_name

J Biotechnol

journal_title

Journal of biotechnology

authors

Hasunuma T,Fukusaki E,Kobayashi A

doi

10.1016/j.jbiotec.2004.04.015

subject

Has Abstract

pub_date

2004-08-05 00:00:00

pages

241-51

issue

3

eissn

0168-1656

issn

1873-4863

pii

S0168165604001889

journal_volume

111

pub_type

杂志文章

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