Abstract:
:Quiescent T lymphocytes reorganize the actin cytoskeleton subsequent to interaction with antigen presenting cells bearing the appropriate peptide antigen. Although both biochemical and genetic evidence indicate that T cell receptor-dependent cytoskeletal reorganization is critical to T cell activation, the mechanisms that mediate this process remain poorly defined. In this study, the cytoskeletal regulatory protein alpha-adducin was identified as a novel target of TCR signaling in primary T lymphocytes through the biochemical purification of an unknown 120 kDa protein (p120) defined by a fortuitously cross-reactive phospho-sensitive antiserum. The epitope identified by this antiserum defines a previously unrecognized site of phosphorylation localized to amino acids 590-620. Both TCR cross-linking and exposure to phorbol ester resulted in the phosphorylation-dependent elimination of this epitope. However, while phorbol ester induced rapid phosphorylation of both the phospho-sensitive epitope and the functionally defined major protein kinase C site present near the carboxy-terminus (serine 724) of alpha-adducin, only the phospho-sensitive epitope was modified upon activation through the TCR. Moreover, inhibition of actin polymerization by cytochalasin D blocked this modification. Of particular importance, alpha-adducin was not expressed in T cell lines, was completely down-regulated in primary T cells within 24h of activation and was reduced in quiescent memory T cells. These results suggest a model in which reorganization of the unique cytoskeletal network that defines a primary quiescent T lymphocyte is mediated in part through the TCR-dependent modification and subsequent down-regulation of alpha-adducin, thereby resulting in a cytoskeletal architecture compatible with T cell effector and memory functions.
journal_name
Mol Immunoljournal_title
Molecular immunologyauthors
Lu Q,Liu X,Trama J,Roti MA,Go WY,Ho SNdoi
10.1016/j.molimm.2004.03.028subject
Has Abstractpub_date
2004-06-01 00:00:00pages
435-47issue
4eissn
0161-5890issn
1872-9142pii
S0161589004000926journal_volume
41pub_type
杂志文章abstract::A bovine papilloma virus-derived vector was used to direct the high level expression in mouse C127 cells of three different cDNAs encoding the human interleukin-2 receptor. These were: the previously described cDNA clone isolated from the T-cell lymphoma, HUT-102; a cDNA clone isolated from mitogen-activated, normal p...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/0161-5890(86)90123-9
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journal_title:Molecular immunology
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doi:10.1016/j.molimm.2008.01.035
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更新日期:1989-04-01 00:00:00
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journal_title:Molecular immunology
pub_type: 历史文章,杂志文章,评审
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更新日期:2015-11-01 00:00:00
abstract::Low molecular weight hemorrhagins were purified from crude Bothrops atrox snake venom by gel filtration followed by ionic strength chromatography. The protein fractions obtained, designated HI-1 to HI-8, contained proteins with molecular masses lower than 30 kDa. HI-5, the most representative among of these fractions,...
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abstract::Interleukin 1 receptor-associated kinase 4 (IRAK4) is a key factor in TLR-mediated host immune function. In this study, an IRAK4 homologue molecule was identified from Apostichopus japonicus (designated as AjIRAK4) by RACE approach. The full-length cDNA of AjIRAK4 was of 2024 bp containing an open reading frame of 131...
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pub_type: 杂志文章
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pub_type: 杂志文章
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更新日期:1987-06-01 00:00:00
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journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2009.07.018
更新日期:2009-10-01 00:00:00
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pub_type: 杂志文章
doi:10.1016/0161-5890(83)90029-9
更新日期:1983-04-01 00:00:00
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pub_type: 杂志文章
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pub_type: 杂志文章
doi:10.1016/0161-5890(84)90069-5
更新日期:1984-06-01 00:00:00
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journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2004.12.011
更新日期:2005-07-01 00:00:00
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journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2007.10.024
更新日期:2008-04-01 00:00:00
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pub_type: 杂志文章
doi:10.1016/j.molimm.2018.08.001
更新日期:2018-09-01 00:00:00
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journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2017.08.025
更新日期:2017-11-01 00:00:00
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journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/0161-5890(86)90115-x
更新日期:1986-05-01 00:00:00
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journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/0161-5890(90)90044-z
更新日期:1990-04-01 00:00:00
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journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2010.08.003
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pub_type: 杂志文章
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pub_type: 杂志文章
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journal_title:Molecular immunology
pub_type: 杂志文章
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更新日期:1992-03-01 00:00:00
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pub_type: 杂志文章
doi:10.1016/0161-5890(91)90087-z
更新日期:1991-01-01 00:00:00
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journal_title:Molecular immunology
pub_type: 杂志文章
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更新日期:2007-03-01 00:00:00
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pub_type: 杂志文章
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更新日期:2017-07-01 00:00:00
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journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/0161-5890(87)90001-0
更新日期:1987-09-01 00:00:00
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pub_type: 杂志文章
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更新日期:1988-02-01 00:00:00
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pub_type: 杂志文章
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更新日期:1990-03-01 00:00:00
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