当前位置： SCI文献检索 > BIOLOGICAL & PHARMACEUTICAL BULLETIN期刊下所有文献 > Bovine 5-oxo-L-prolinase: simple assay method, purification, cDNA cloning, and detection of mRNA in the coronary artery.

Bovine 5-oxo-L-prolinase: simple assay method, purification, cDNA cloning, and detection of mRNA in the coronary artery.

Abstract：

:RS-7897 is a novel antianginal nitrate containing an L-2-oxothiazolidine-4-carboxylic acid (OTCA) and unlike other organic nitrates does not induce nitrate tolerance. OTCA is known to be converted to L-cysteine (L-Cys) by rat 5-oxo-prolinase (5-OPase) and was detected in the plasma of RS-7897-treated dogs. Nitrate tolerance is considered to develop mainly through sulfhydryl depletion. We hypothesized that RS-7897-derived OTCA was converted into L-Cys by 5-OPase and supplied sulfhydryl groups in vascular smooth muscle cells, the targets of the nitrate. As the initial step for clarifying the presumed role of 5-OPase in RS-7897 metabolism, we established a non-radiochemical assay method highly specific for 5-OPase. Using this assay method, we purified 5-OPase from bovine kidney cytosol until homogeneous results were obtained in sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). The catalytic activity of bovine 5-OPase to convert OTCA to L-Cys was confirmed, as was the case for the rat enzyme. Then the cDNA encoding bovine 5-OPase was cloned. The deduced amino acid sequence revealed that bovine 5-OPase was highly homologous to rat 5-OPase. Based on the bovine cDNA sequence, oligonucleotide primers were synthesized and used for RT-PCR. 5-OPase mRNA was significantly detected in the RT-PCR product of the bovine coronary artery, a major target organ of RS-7897. These results suggest that OTCA may be converted to L-Cys by 5-OPase in the mammalian coronary artery when treated with RS-7897, and thus generated L-Cys may reduce sulfhydryl depletion and contribute to the prevention of the development of nitrate tolerance.

journal_name

Biol Pharm Bull

journal_title

Biological & pharmaceutical bulletin

authors

Watanabe T,Abe K,Ishikawa H,Iijima Y

doi

10.1248/bpb.27.288

subject

Has Abstract

pub_date

2004-03-01 00:00:00

pages

288-94

issue

3

eissn

0918-6158

issn

1347-5215

journal_volume

27

pub_type

杂志文章

相关文献

BIOLOGICAL & PHARMACEUTICAL BULLETIN文献大全
  • Proteomic analysis of the effects of tetramethylpyrazine on irradiated QXMSC1 cells.

    abstract：:Tetramethylpyrazine is the active ingredient of a Chinese herbal medicine. In this study, tetramethylpyrazine was tested for its activities in irradiated bone marrow stromal QXMSC1 cells. The proliferation of QXMSC1 cells was measured by MTS assay kit and flow cytometry. To identify proteins involved in the processes ...

    journal_title：Biological & pharmaceutical bulletin

    pub_type： 杂志文章

    doi：10.1248/bpb.30.397

    authors： Ma ZC,Tan HL,Xiao CR,Wang YG,Wang SQ,Gao Y

    更新日期：2007-02-01 00:00:00

  • An increase of 63 kDa-protein present in the cell membranes of Staphylococcus aureus that bears a plasmid mediating inducible resistance to partial macrolide and streptogramin B antibiotics.

    abstract：:A plasmid, pEP2104 (23.9 kilobase pairs), from Staphylococcus aureus carries a gene that specifies inducible resistance to 14-membered (erythromycin, EM, and oleandomycin, OL) and 16-membered macrolide (mycinamicin I and II), but not to all of the latter, and to streptogramin type B antibiotics (partial macrolide- and...

    journal_title：Biological & pharmaceutical bulletin

    pub_type： 杂志文章

    doi：10.1248/bpb.16.1288

    authors： Matsuoka M,Jánosi L,Endou K,Saitoh S,Hashimoto H,Nakajima Y

    更新日期：1993-12-01 00:00:00

  • The therapeutic efficacy of VP-343, a selective vasopressin V2 receptor antagonist, in the experimental SIADH rat model.

    abstract：:The aim of this work is to investigate the therapeutic efficacy of VP-343 ((N-[4-[[(2S,3aR)-2-hydroxy-2,3,3a,4-tetrahydropyrrolo[1,2-a]qunoxalin-5(1H)-yl]phenyl]-4'-methyl[1,1'-biphenyl]-2-carboxamide), a selective vasopressin V2 receptor antagonist, using the experimental SIADH (syndrome of inappropriate secretion of...

    journal_title：Biological & pharmaceutical bulletin

    pub_type： 杂志文章

    doi：10.1248/bpb.23.1323

    authors： Naito A,Hasegawa H,Kurasawa T,Ohtake Y,Matsukawa H,Ezure Y,Tsuriya Y,Koike K,Shigenobu K

    更新日期：2000-11-01 00:00:00

  • Vasodilator effects of ibudilast on retinal blood vessels in anesthetized rats.

    abstract：:Ibudilast (3-isobutyryl-2-isopropylpyrazolo[1,5-alpha]pyridine) is clinically used as a cerebral vasodilator in Japan. However, the effects of ibudilast on retinal blood vessels have not been fully examined. The aim of this study, therefore, was to examine the effects of ibudilast on retinal blood vessels in rats in v...

    journal_title：Biological & pharmaceutical bulletin

    pub_type： 杂志文章

    doi：10.1248/bpb.32.1924

    authors： Noguchi M,Mori A,Sakamoto K,Nakahara T,Ishii K

    更新日期：2009-11-01 00:00:00

  • Ferulic acid attenuates adhesion molecule expression in gamma-radiated human umbilical vascular endothelial cells.

    abstract：:Radiation induces an important inflammatory response in the irradiated organs, characterized by leukocyte infiltration and vascular changes. Since adhesion molecules play an important role in facilitating the immune response at the inflammation sites, interfering with the expression of these molecules may be an import...

    journal_title：Biological & pharmaceutical bulletin

    pub_type： 杂志文章

    doi：10.1248/bpb.33.752

    authors： Ma ZC,Hong Q,Wang YG,Tan HL,Xiao CR,Liang QD,Cai SH,Gao Y

    更新日期：2010-01-01 00:00:00

  • pH-dependent inhibitory effects of angiotensin-converting enzyme inhibitors on cefroxadine uptake by rabbit small intestinal brush-border membrane vesicles and their relationship with hydrophobicity and the ratio of zwitterionic species.

    abstract：:The inhibitory effects of five angiotensin-converting enzyme (ACE) inhibitors on the uptake of an aminocephalosporin antibiotic, cefroxadine, by rabbit small intestinal brush-border membrane vesicles were examined in the presence of an inward H+ gradient. Dixon plot analysis showed that all these ACE inhibitors inhibi...

    journal_title：Biological & pharmaceutical bulletin

    pub_type： 杂志文章

    doi：10.1248/bpb.22.721

    authors： Kitagawa S,Takeda J,Sato S

    更新日期：1999-07-01 00:00:00

  • Imaging analysis of insulin secretion with two-photon microscopy.

    abstract：:High-resolution deep tissue imaging is possible with two-photon excitation microscopy. With the combined application of two-photon imaging and perfusion with a polar fluorescent tracer, we have established a method to detect exocytic events inside secretory tissues. This method displays the spatiotemporal distribution...

    journal_title：Biological & pharmaceutical bulletin

    pub_type： 杂志文章,评审

    doi：10.1248/bpb.b14-00880

    authors： Takahashi N

    更新日期：2015-01-01 00:00:00

  • Anti-atherogenic effects of the methanol extract of Sorbus cortex in atherogenic-diet rats.

    abstract：:The present study was designed to examine whether the methanol extract of Sorbus commixta cortex (MSC) could prevent the development of atherosclerosis through regulating the vascular nitric oxide (NO) and endothelin-1 (ET-1) systems in atherogenic-diet rats. Our findings show that aortic NO production as well as endo...

    journal_title：Biological & pharmaceutical bulletin

    pub_type： 杂志文章

    doi：10.1248/bpb.28.1444

    authors： Sohn EJ,Kang DG,Mun YJ,Woo WH,Lee HS

    更新日期：2005-08-01 00:00:00

  • Expression and regulation of breast cancer resistance protein and multidrug resistance associated protein 2 in BALB/c mice.

    abstract：:Microsomal enzyme inducers are known to influence the expression of many transporter proteins and mRNA. In this study, we examined the effects of microsomal enzyme inducers on the mRNA expression of Breast Cancer Resistance Protein (BCRP) and Multidrug Resistance Associated Protein 2 (MRP2) in BALB/c mice. mRNA expres...

    journal_title：Biological & pharmaceutical bulletin

    pub_type： 杂志文章

    doi：10.1248/bpb.29.1032

    authors： Han Y,Sugiyama Y

    更新日期：2006-05-01 00:00:00

  • A sensitive ELISA for the characterization of two forms of circulating intercellular adhesion molecule-1 in human plasma.

    abstract：:Nine murine monoclonal antibodies (MAbs) raised against recombinant soluble intercellular adhesion molecule-1 (sICAM-1) were evaluated by means of ELISAs, and their neutralizing activity was investigated in two bioassays employing Raji cells activated with phorbol myristate acetate. Three of the MAbs inhibited autoagg...

    journal_title：Biological & pharmaceutical bulletin

    pub_type： 杂志文章

    doi：10.1248/bpb.18.1036

    authors： Inuzuka H,Seita T,Okamoto K,Iida K,Ogawa Y,Iwasa S

    更新日期：1995-08-01 00:00:00

  • Dietary supplementation of L-carnosine prevents ischemia/reperfusion-induced renal injury in rats.

    abstract：:The effects of dietary supplementation of L-carnosine (beta-alanyl-L-histidine) on ischemia/reperfusion-induced acute renal failure (ARF) in rats were examined. Ischemic ARF was induced by occlusion of the left renal artery and vein for 45 min followed by reperfusion, 2 weeks after contralateral nephrectomy. Renal fun...

    journal_title：Biological & pharmaceutical bulletin

    pub_type： 杂志文章,收录出版

    doi：10.1248/bpb.28.361

    authors： Fujii T,Takaoka M,Tsuruoka N,Kiso Y,Tanaka T,Matsumura Y

    更新日期：2005-02-01 00:00:00

  • Purification and characterization of ginsenoside Ra-hydrolyzing beta-D-xylosidase from Bifidobacterium breve K-110, a human intestinal anaerobic bacterium.

    abstract：:Beta-D-Xylosidase (EC 3.2.1.37) has been purified from ginsenoside Ra-metabolizing Bifidobacterium breve K-110, which was isolated from human intestinal microflora. beta-D-Xylosidase was purified to apparent homogeneity by a combination of ammonium sulfate precipitation, QAE-cellulose, butyl-toyopearl, hydroxyapatit a...

    journal_title：Biological & pharmaceutical bulletin

    pub_type： 杂志文章

    doi：10.1248/bpb.26.1170

    authors： Shin HY,Lee JH,Lee JY,Han YO,Han MJ,Kim DH

    更新日期：2003-08-01 00:00:00

  • Role of intracellular defense factors against methylmercury toxicity.

    abstract：:Methylmercury (MeHg) is a causative agent of Minamata disease and an environmental pollutant that comprises a large portion of organically occurring mercury. Many aspects of the biological defense mechanisms against MeHg toxicity remain unclear. Recently, nuclear factor-E2-related factor 2 (Nrf2), heat shock factor pr...

    journal_title：Biological & pharmaceutical bulletin

    pub_type： 杂志文章,评审

    doi：10.1248/bpb.b212019

    authors： Hwang GW

    更新日期：2012-01-01 00:00:00

  • Antinociceptive activities of 70% methanol extract of evodiae fructus (fruit of Evodia rutaecarpa var. bodinieri) and its alkaloidal components.

    abstract：:The effects of 70% methanol extract (EA-ext) from Evodiae Fructus (EA) consisting of dried fruits of Evodia rutaecarpa var. bodinieri (Rutaceae) on nociceptive responses were investigated. Oral administration of 50 or 200 mg/kg EA-ext had the same antinociceptive effect on writhing responses as induced by acetic acid....

    journal_title：Biological & pharmaceutical bulletin

    pub_type： 杂志文章

    doi：10.1248/bpb.20.243

    authors： Matsuda H,Wu JX,Tanaka T,Iinuma M,Kubo M

    更新日期：1997-03-01 00:00:00

  • The relationship between the plasma concentration of bepridil and its efficacy in the treatment of atrial fibrillation in Japanese patients.

    abstract：:Bepridil hydrochloride is used for treatment of atrial fibrillation (AF) in Japan. We investigated the relationship between plasma concentrations of bepridil just before dosing (Cbep) and its clinical efficacy in Japanese patients (n=36) with AF. Patients were treated orally with 100, 150 or 200 mg/d bepridil. Cbep we...

    journal_title：Biological & pharmaceutical bulletin

    pub_type： 临床试验,杂志文章

    doi：10.1248/bpb.35.672

    authors： Noda K,Gotoh Y,Tanioka S,Narayama Y,Kobayashi M,Iwai S,Katoh N,Tadano K

    更新日期：2012-01-01 00:00:00

  • Effect of Usuhiratake (Pleurotus pulmonarius) on sneezing and nasal rubbing in BALB/c mice.

    abstract：:The anti-rhinitis properties of Pleurotus pulmonarius were investigated in BALB/c mice. A single administration of Pleurotus Pulmonarius caused no significant effect on antigen-induced nasal rubbing and sneezing at a dose of 500 mg/kg, but a significant inhibition was observed after 2 weeks of repeated treatment at th...

    journal_title：Biological & pharmaceutical bulletin

    pub_type： 杂志文章

    doi：10.1248/bpb.30.1557

    authors： Yatsuzuka R,Nakano Y,Jiang S,Ueda Y,Kishi Y,Suzuki Y,Yokota E,Rahman A,Ono R,Kohno I,Kamei C

    更新日期：2007-08-01 00:00:00

  • Antidepressant-like effects of psoralen isolated from the seeds of Psoralea corylifolia in the mouse forced swimming test.

    abstract：:The forced swimming test (FST) is suggested to produce abnormalities in the serotonergic and hypothalamic-pituitary-adrenal (HPA) axis systems. Therefore, compounds that attenuate these neurobiological alterations may have potential as antidepressants. The behavioral and biochemical effects of psoralen, a major furoco...

    journal_title：Biological & pharmaceutical bulletin

    pub_type： 杂志文章

    doi：10.1248/bpb.31.1109

    authors： Xu Q,Pan Y,Yi LT,Li YC,Mo SF,Jiang FX,Qiao CF,Xu HX,Lu XB,Kong LD,Kung HF

    更新日期：2008-06-01 00:00:00

  • Studies of drug delivery systems for a therapeutic agent used in osteoporosis. I. Pharmacodynamics (hypocalcemic effect) of elcatonin in rabbits following rectal administration of hollow-type suppositories containing elcatonin.

    abstract：:In this study, we developed a new hollow-type suppository containing elcatonin ((Asu1,7)-eel calcitonin, ECT), a synthetic derivative of eel calcitonin, which produces hypocalcemia, as a pharmaceutical preparation for self administration, to be used instead of parenteral injections for patients with osteoporosis. The ...

    journal_title：Biological & pharmaceutical bulletin

    pub_type： 杂志文章

    doi：10.1248/bpb.21.1187

    authors： Watanabe Y,Mizufune Y,Kubomura A,Kiriyama M,Utoguchi N,Matsumoto M

    更新日期：1998-11-01 00:00:00

  • Antiallergic, antipyretic, hypoglycemic and hepatoprotective effects of aqueous extract of Coronopus didymus Linn.

    abstract：:The aqueous extract of whole plant of Coronopus didymus LINN (CD) [Family: Bracicacea] was screened for antiallergic, antipyretic and hepatoprotective effects in rats and hypoglycemic activity in mice. The extract showed significant antiallergic, antipyretic, hypoglycemic and hepatoprotective activity at 200 and 400 m...

    journal_title：Biological & pharmaceutical bulletin

    pub_type： 杂志文章

    doi：10.1248/bpb.28.468

    authors： Mantena SK,Mutalik S,Srinivasa H,Subramanian GS,Prabhakar KR,Reddy KR,Srinivasan KK,Unnikrishnan MK

    更新日期：2005-03-01 00:00:00

  • F1 hybrid mice (BALB/c x DBA/1) are resistant to collagen-induced arthritis with beta-glucan as an adjuvant.

    abstract：:Collagen-induced arthritis (CIA) is an experimental model of rheumatoid arthritis (RA) used for studying the clinical, immunological and genetic factors of the disease. Many studies of genetic susceptibility to CIA have been performed, usually with two strains of mice, DBA/1 and B10.RIII, since they are highly suscept...

    journal_title：Biological & pharmaceutical bulletin

    pub_type： 杂志文章

    doi：10.1248/bpb.29.1300

    authors： Hida S,Nagi-Miura N,Adachi Y,Ohno N

    更新日期：2006-06-01 00:00:00

  • Effects of pine needle extract on differentiation of 3T3-L1 preadipocytes and obesity in high-fat diet fed rats.

    abstract：:The present study examined the anti-obesity effects of pine needle extract (PNE) in 3T3-L1 preadipocytes and in vivo studies. PNE treatment suppressed both glycerol-3-phosphate dehydrogenase activity and expression of peroxisome proliferator-activated receptor (PPAR) gamma in cultured 3T3-L1 adipocytes. To investigate...

    journal_title：Biological & pharmaceutical bulletin

    pub_type： 杂志文章

    doi：10.1248/bpb.29.2111

    authors： Jeon JR,Kim JY

    更新日期：2006-10-01 00:00:00

  • Enhancement of the vanadate-stimulated release of lipoprotein lipase activity by astilbin from the leaves of Engelhardtia chrysolepis.

    abstract：:Astilbin, a dihydroflavonol rhamnoside isolated from the leaves of Engelhardtia chrysolepis, enhanced the vanadate-stimulated release of lipoprotein lipase (LPL) activity from rat isolated fat pads. N-[2-(Methyl-amino)ethyl]-5-isoquinolinesulfonamide (H-8), a potent inhibitor of cAMP-dependent protein kinase (PKA), ma...

    journal_title：Biological & pharmaceutical bulletin

    pub_type： 杂志文章

    doi：10.1248/bpb.21.517

    authors： Motoyashiki T,Miyake M,Morita T,Mizutani K,Masuda H,Ueki H

    更新日期：1998-05-01 00:00:00

  • Suppression of formalin-induced nociception by cilnidipine, a voltage-dependent calcium channel blocker.

    abstract：:Cilnidipine is a 1,4-dihydropyridine-derived voltage-dependent calcium channel (VDCC) blocker and suppresses N-type VDCC currents in addition to L-type VDCC currents. An earlier investigation has suggested that intrathecally injected cilnidipine produces antinociception by blocking N-type VDCCs in mice. The present st...

    journal_title：Biological & pharmaceutical bulletin

    pub_type： 杂志文章

    doi：10.1248/bpb.32.1695

    authors： Koganei H,Shoji M,Iwata S

    更新日期：2009-10-01 00:00:00

  • Dehydrocholic Acid Ameliorates Sodium Taurocholate-Induced Acute Biliary Pancreatitis in Mice.

    abstract：:Acute biliary pancreatitis (ABP) with a high mortality rate is an incurable digestive system disease induced by abnormal bile acid regurgitation due to the biliary obstruction. Dehydrocholic acid (DA) alleviates the severity of cholestatic hepatitis related to biliary inflammation, suggesting DA is potential to develo...

    journal_title：Biological & pharmaceutical bulletin

    pub_type： 杂志文章

    doi：10.1248/bpb.b20-00021

    authors： Zhang X,Xin G,Li S,Wei Z,Ming Y,Yuan J,Wen E,Xing Z,Yu K,Li Y,Zhang J,Zhang B,Niu H,Huang W

    更新日期：2020-01-01 00:00:00

  • Antimalarial activity and nucleoside transport inhibitory activity of the triterpenic constituents of Cimicifuga spp.

    abstract：:The in vitro antimalarial activity against human malaria parasite (Plasmodium falciparum, FCR-3 strain) was examined using 59 triterpenoids obtained during studies on the triterpenic constituents of Cimicifuga spp. The 50% effective concentration values (EC50) of 25 active triterpenoids were 1.0-3.0 microM, and 19 of ...

    journal_title：Biological & pharmaceutical bulletin

    pub_type： 杂志文章

    doi：10.1248/bpb.21.823

    authors： Takahira M,Kusano A,Shibano M,Kusano G,Koizumi K,Suzuki R,Kim HS,Wataya Y

    更新日期：1998-08-01 00:00:00

  • A chromatographic approach for elevating the antibody-dependent cellular cytotoxicity of antibody composites.

    abstract：:Recent studies have shown that antibodies with low fucose content in their oligosaccharides exhibit highly potent antibody-dependent cellular cytotoxicity (ADCC). However, composites of therapeutic antibodies produced by conventional production systems using cell lines such as Chinese hamster ovary (CHO) and SP2/0 do ...

    journal_title：Biological & pharmaceutical bulletin

    pub_type： 杂志文章

    doi：10.1248/bpb.32.1604

    authors： Tojo S,Okazaki A,Wakitani M,Shinkawa T,Uchida K,Suzawa T

    更新日期：2009-09-01 00:00:00

  • Gray-scale liver enhancement with Sonazoid (NC100100), a novel ultrasound contrast agent; detection of hepatic tumors in a rabbit model.

    abstract：:The liver contrast effects of Sonazoid in two ultrasonographic imaging modes, gray-scale conventional and harmonic, were examined as a time-related study in normal rabbits, and evaluated quantitatively and visually with tumor-model rabbits to estimate the diagnostic potential. Peak enhancement of vessels and parenchym...

    journal_title：Biological & pharmaceutical bulletin

    pub_type： 杂志文章

    doi：10.1248/bpb.26.1272

    authors： Watanabe R,Matsumura M,Chen CJ,Kaneda Y,Ishihara M,Fujimaki M

    更新日期：2003-09-01 00:00:00

  • Difference in rectal absorption of morphine from hollow-type and conventional suppositories in rabbits.

    abstract：:The bioavailability of morphine after rectal administration using three types of suppositories containing morphine hydrochloride (10 mg) in different added forms was evaluated in rabbits. Three types of suppositories were constructed with a base material (Witepsol H-15): a conventional suppository containing morphine ...

    journal_title：Biological & pharmaceutical bulletin

    pub_type： 杂志文章

    doi：10.1248/bpb.16.150

    authors： Matsumoto Y,Watanabe Y,Yamamoto I,Matsumoto M

    更新日期：1993-02-01 00:00:00

  • 4-Hydroxykobusin inhibits the induction of nitric oxide synthase by inhibiting NF-kappaB and AP-1 activation.

    abstract：:We recently isolated a novel lignan, 4-hydroxykobusin from Geranium thunbergii (Liu et al., Arch. Pharm. Res., 29, 1109-1113, 2006). Here, we studied its effect on the expression of inducible nitric oxide synthase (iNOS) gene in RAW264.7 cells. 4-Hydroxykobusin inhibited nitric oxide (NO) production in a concentration...

    journal_title：Biological & pharmaceutical bulletin

    pub_type： 杂志文章

    doi：10.1248/bpb.30.1097

    authors： Pokharel YR,Liu QH,Oh JW,Woo ER,Kang KW

    更新日期：2007-06-01 00:00:00

  • Neuroprotective effects of phenylethanoid glycosides from Cistanches salsa against 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP)-induced dopaminergic toxicity in C57 mice.

    abstract：:The neurotoxin 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) has been employed to create a Parkinson's disease-like model in both rodents and primates based primarily on its ability to create a striatal dopamine deficit due to the loss of dopaminergic neurons in the substantia nigra compacta. The present study w...

    journal_title：Biological & pharmaceutical bulletin

    pub_type： 杂志文章

    doi：10.1248/bpb.27.797

    authors： Geng X,Song L,Pu X,Tu P

    更新日期：2004-06-01 00:00:00