Abstract:
:We recently isolated a novel lignan, 4-hydroxykobusin from Geranium thunbergii (Liu et al., Arch. Pharm. Res., 29, 1109-1113, 2006). Here, we studied its effect on the expression of inducible nitric oxide synthase (iNOS) gene in RAW264.7 cells. 4-Hydroxykobusin inhibited nitric oxide (NO) production in a concentration-dependent manner and blocked the lipopolysaccharide (LPS)-induced expression of inducible nitric oxide synthase (iNOS). To identify the mechanistic basis for its inhibition of iNOS induction, we examined the effect of 4-hydroxykobusin on the transactivation of iNOS gene by luciferase reporter activity using -1.59 kb flanking region. The lignan suppressed the reporter gene activity and the LPS-induced reporter activations of nuclear factor-kappaB (NF-kappaB) and activator protein-1 (AP-1) were also significantly blocked by 4-hydroxykobusin. These findings suggest that the inhibition of LPS-induced NO formation by 4-hydroxykobusin is due to its inhibition of NF-kappaB and AP-1 activation.
journal_name
Biol Pharm Bulljournal_title
Biological & pharmaceutical bulletinauthors
Pokharel YR,Liu QH,Oh JW,Woo ER,Kang KWdoi
10.1248/bpb.30.1097subject
Has Abstractpub_date
2007-06-01 00:00:00pages
1097-101issue
6eissn
0918-6158issn
1347-5215pii
JST.JSTAGE/bpb/30.1097journal_volume
30pub_type
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