Seed protein variation among pepper (Capsicum sp.) genotypes revealed by MALDI-TOF analysis.

abstract：BACKGROUND:Reversibly glycosylated polypeptide (RGP), a kind of hydrosoluble and plasmodesmal-associated protein found in plants, plays a crucial role in the development of pollen. OBJECTIVE:A novel RGP 2 was isolated and identified from rape (Brassica napus L.) bee pollen. METHOD:RGP2 was isolated and purified by io...

journal_title：Protein and peptide letters

authors： Zhang Q,Sun T,Tuo X,Li Y,Yang H,Deng J

更新日期：2020-11-03 00:00:00

abstract：:The high technical standard of current peptide chemistry, which has evolved over the past three decades, has profoundly facilitated the investigation of proteins and their interactions with other molecules at the level of individual amino acids. Using currently available peptide synthesis methods, sequentially continu...

journal_title：Protein and peptide letters

authors： Eichler J

更新日期：2004-08-01 00:00:00

abstract：:This study revealed that the content of protein S29 in ribosomes of cancer cell line A549 was distinctly low (equivalent to about 30% of that of 2BS). The conclusion was acquired based on the ratios of spot volume of ribosomal protein S29 to that of several other ribosomal proteins (S29/L37a, S29/L38, S29/S27 and S29/...

journal_title：Protein and peptide letters

authors： Zhou ZD,Bao L,Liu DG,Li MQ,Ge YZ,Huang YL,Liu WY

更新日期：2003-02-01 00:00:00

abstract：:A halophilic nucleoside diphosphate kinase from a moderate halophile, Halomonas sp. 593 (593NDK), was found to be resistant to heat treatment, as indicated by the high level of activity recovery after heating at high temperatures. This is due to reversibility of thermal unfolding, not the high melting temperature, of ...

journal_title：Protein and peptide letters

authors： Tokunaga H,Oda Y,Yonezawa Y,Arakawa T,Tokunaga M

更新日期：2006-01-01 00:00:00

abstract：BACKGROUND:Radical Probe Mass Spectrometry (RP-MS) describes a pioneering methodology in structural biology that enables the study of protein structures, their interactions, and dynamics on fast timescales (down to sub-milliseconds). Hydroxyl radicals (•OH) generated directly from water within aqueous solutions induce ...

journal_title：Protein and peptide letters

authors： Maleknia SD,Downard KM

更新日期：2019-01-01 00:00:00

abstract：:VML is a lectin from Vatairea macrocarpa seeds that has various biological activities. Here, we describe three new lectin isoforms from V. macrocarpa identified through genomic DNA analysis. One of these isoforms has high similarity to VML, while another that has noteworthy differences. We have denoted the new isoform...

journal_title：Protein and peptide letters

authors： Alves Filho JG,do Nascimento AS,Gondim AC,Pereira RH,da Cunha RM,Nagano CS,Teixeira EH,Nascimento KS,Cavada BS

更新日期：2013-11-01 00:00:00

abstract：:In this paper(1) we present a novel framework for protein secondary structure prediction. In this prediction framework, firstly we propose a novel parameterized semi-probability profile, which combines single sequence with evolutionary information effectively. Secondly, different semi-probability profiles are respecti...

journal_title：Protein and peptide letters

authors： Huang DS,Huang X

更新日期：2006-01-01 00:00:00

abstract：:Mutations of tumor suppressor protein p53 are present in almost about 50% of all cancers. It has been reported that the p53 mutations cause aggregation and subsequent loss of p53 function, leading to cancer progression. Here in this study we focus on the inhibitory effects of cationic osmolyte molecules acetylcholine ...

journal_title：Protein and peptide letters

authors： Chen Z,Kanapathipillai M

更新日期：2017-01-01 00:00:00

abstract：:Glycosyltransferases (GTs) are a ubiquitous group of enzymes that catalyze the transfer of sugar moieties from activated donor molecules to specific acceptor molecules, forming glycosidic bonds. Nucleotide-sugars, lipid phosphate sugars and phosphate sugars can act as activated donor molecules while acceptor substrate...

journal_title：Protein and peptide letters

authors： Gomez-Casati DF,Martín M,Busi MV

更新日期：2013-08-01 00:00:00

abstract：:S-adenosyl-l-homocysteine hydrolase from a malaria parasite Plasmodium falciparum (PfSAHH) has been crystallized by the vapor diffusion method. The crystals belong to an orthorhombic space group P212121 with the cell dimensions of a = 76.66 A, b = 86.31 A, and c = 335.6 A. There are four subunits (one tetramer) per as...

journal_title：Protein and peptide letters

authors： Tanaka N,Kusakabe Y,Shiraiwa K,Sakamoto Y,Nakanishi M,Kitade Y,Nakamura KT

更新日期：2004-04-01 00:00:00

abstract：:We previously described a potent anthrax toxin inhibitor, based on a phage-library-selected peptide sequence, synthesized as a tetra-branched molecule on a lysine core and further modified for improvement of activity [Pini et al., Biochem. J., 2006, 395, 157]. This branched peptide had very low solubility because of s...

journal_title：Protein and peptide letters

authors： Pini A,Brunetti J,Falciani C,Fabbrini M,Bracci L

更新日期：2008-01-01 00:00:00

abstract：BACKGROUND:OPT3s are involved in the transport of Fe from xylem to phloem, in loading Fe into phloem, and in the transmission of shoot-to-root iron signaling. Yet, apart from Arabidopsis, little is known about these transporters'functions in other plant species. OBJECTIVE:OPT3 proteins of several plant species were ch...

journal_title：Protein and peptide letters

authors： Kurt F

更新日期：2020-06-25 00:00:00

abstract：BACKGROUND:Microbe-Binding Peptides (MBPs) are currently being investigated to address the problem of antimicrobial resistance. Strategies enhancing their antimicrobial activity have been developed, including peptide dimerization. Here, we present an alternative approach based on peptide polymerization, yielding hapten...

journal_title：Protein and peptide letters

authors： Climacosa FMM,King RAN,Santos BMM,Caoili SEC

更新日期：2020-01-01 00:00:00

abstract：:The development of a stable L-BDH chimera was attempted by exchanging whole domains between two native structural analogs, L-BDH and meso-BDH, because the S-configuration specificity of L-BDH is valuable from the standpoint of its application but its activity is unstable, whereas meso-BDH is stable. The domain chimera...

journal_title：Protein and peptide letters

authors： Shimegi T,Takusagawa Y,Ohtsuki T,Noda S,Kurisu G,Kusunoki M,Ui S

更新日期：2011-08-01 00:00:00

abstract：:Apelin, an endogenous ligand of the G-protein-coupled receptor APJ, is a novel myokine and may play a key role in regulating energy metabolism. The purpose of the present study was to investigate the effects of hypoxic exposure, exercise, and hypoxic exercise training on the expression of apelin and APJ in skeletal mu...

journal_title：Protein and peptide letters

authors： Ji W,Gong L,Wang J,He H,Zhang Y

更新日期：2017-01-01 00:00:00

abstract：:A new galactose-specific lectin, named BBL, was purified from seeds of Bauhinia bauhinioides by precipitation with ammonium sulfate, followed by two steps of ion exchange chromatography. BBL haemagglutinated rabbit erythrocytes (native and treated with proteolytic enzymes) showing stability even after exposure to 60 °...

journal_title：Protein and peptide letters

authors： Silva HC,Bari AU,Pereira-Jénior FN,Simões RC,Barroso-Neto IL,Nobre CB,Pereira MG,Nascimento KS,Rocha BA,Delatorre P,Nagano CS,Assreuy AM,Cavada BS

更新日期：2011-04-01 00:00:00

abstract：:Human ankyrin repeat and suppressor of cytokine signaling box protein 9 (hASB9), a subunit of an Elongin C-cullin-SOCS box (ECS) E3 ubiquitin ligase complex, is believed to be involved in specific substrate-recognition for ubiquitination and degradation. In fact, this specific substrate-recognition is determined by th...

journal_title：Protein and peptide letters

authors： Fei X,Zhang Y,Gu X,Qiu R,Mao Y,Ji C

更新日期：2009-01-01 00:00:00

abstract：:Globulins are a major class of seed storage proteins which were thought to be enzymatically inactive. These proteins belong to the most ancient cupin superfamily. They can be graded into 11S legumin type and 7S vicilin type based on their sedimentation coefficients. Members from both classes share structural homology ...

journal_title：Protein and peptide letters

authors： Kesari P,Neetu,Sharma A,Katiki M,Kumar P,Gurjar BR,Tomar S,Sharma AK,Kumar P

更新日期：2017-01-01 00:00:00

abstract：:In general, halophilic proteins are stable only in the presence of salts at high concentrations. Not only is high salt concentration important for structural stability of halophilic proteins, but also refolding of a denatured halophilic protein requires high salt concentration. This review summarizes the importance of...

journal_title：Protein and peptide letters

authors： Arakawa T,Tokunaga M

更新日期：2004-04-01 00:00:00

abstract：:Cyclophilin A (CyPA) is a cytosolic receptor of immunosuppressive drug cyclosporin A (CsA) which possesses peptidyl-prodyl cis/trans isomerase (PPIase) activity. The recombinant human CyPA (rhCyPA) gene has been expressed in E. coli M15. Purification was performed using salting-out, as well as Sephacryl S-100 and DEAE...

journal_title：Protein and peptide letters

authors： Zhang XC,Wang WD,Wang JS,Pan JC,Zou GL

更新日期：2011-12-01 00:00:00

abstract：:Sexual maturation and olfactory bulb defects found in prokineticin 2 (Pk2) and prokineticin receptor 2 (Pkr2) mutant mice resembling the phenotypic characteristics of Kallmann syndrome (KS), gave rise to the question of whether these genes would have a role in KS pathogenesis. Later, mutations in both genes were ident...

journal_title：Protein and peptide letters

authors： Murcia-Belmonte V,Astillero-López V,Esteban PF

更新日期：2016-01-01 00:00:00

abstract：:Identification on protein folding types is always based on the 27-class folds dataset, which was provided by Ding & Dubchak in 2001. But with the avalanche of protein sequences, fold data is also expanding, so it will be the inevitable trend to improve the existing dataset and expand more folding types. In this paper,...

journal_title：Protein and peptide letters

authors： Liu L,Hu XZ,Liu XX,Wang Y,Li SB

更新日期：2012-04-01 00:00:00

abstract：:We here describe a new strategy for the delivery of oligonucleotides to cells that is based on the use of a short peptide containing three functional units: a membrane-penetrating segment, a DNA-binding domain and a cell-localization sequence. The designed vector binds strongly to oligonucleotides and has membrane-per...

journal_title：Protein and peptide letters

authors： Smith J,Guidry J,Wittung-Stafshede P

更新日期：2003-02-01 00:00:00

abstract：:Bucain, a potent neurotoxin isolated from the venom of the Malayan krait (Bungarus candidus), induces paralysis and death. Its crystal structure has been determined at 2.10 A resolution and based on the molecular topology and hydrophobicity profile is structurally classified as a three-fingered alpha-neurotoxin posses...

journal_title：Protein and peptide letters

authors： Murakami MT,Kini RM,Arni RK

更新日期：2009-01-01 00:00:00

abstract：:We used a de novo designed, beta-hairpin forming T1 peptide as a model to investigate the kinetics of peptide fibrogenesis by a combination of light scattering (LS), circular dichroism (CD), fluorescence, and atomic force microscopy (AFM). The results demonstrate that the T1 fibrogenesis undergoes a consecutive stepwi...

journal_title：Protein and peptide letters

authors： Wang C,Sha Y

更新日期：2010-04-01 00:00:00

abstract：:Spermadhesins are a family of secretory proteins expressed in the male genital tract of pig, horse and bull. Their function and structure have been widely studied, especially those isolated from boar. However, there are no data concerning spermadhesins isolated from buck. Buck seminal plasma was collected and subjecte...

journal_title：Protein and peptide letters

authors： Teixeira DI,Cavada BS,Sampaio AH,Havt A,Bloch C Jr,Prates MV,Moreno FB,Santos EA,Gadelha CA,Gadelha TS,Crisóstomo FS,Freitas VJ

更新日期：2002-08-01 00:00:00

abstract：:Polymyositis is an inflammatory myopathy characterized by muscle invasion of T-cells penetrating the basal lamina and displacing the plasma membrane of normal muscle fibers. In order to understand the different adhesive mechanisms at the T-cell surface, Schubert randomly selected 19 proteins expressed at the T-cell su...

journal_title：Protein and peptide letters

authors： Li FZ,Gao F

更新日期：2012-08-01 00:00:00

abstract：:Prediction of protein structure from its amino acid sequence is still a challenging problem. The complete physicochemical understanding of protein folding is essential for the accurate structure prediction. Knowledge of residue solvent accessibility gives useful insights into protein structure prediction and function ...

journal_title：Protein and peptide letters

authors： Pugalenthi G,Kandaswamy KK,Chou KC,Vivekanandan S,Kolatkar P

更新日期：2012-01-01 00:00:00

abstract：:DNA fragmentation factor 45 (DFF45) regulates DNase DFF40 as its inhibitor and chaperone. It was reported that the N-terminal domain (NTD) of DFF45 alone is disordered and DFF40 is necessary as a mutual chaperone for the folding of NTD. However, here we reported the crystallization of DFF45 NTD. These crystals diffrac...

journal_title：Protein and peptide letters

authors： Li T,Li X,Yang W,Rao Z,Zhai Zh

更新日期：2003-04-01 00:00:00

abstract：:Alpha-gliadins isolated by carboxymethylcellulose chromatography contain noncovalently bound glucose probably due to contaminating proteoglycans and to material shed from the column. Traces of carbohydrate remain strongly bound to alpha-gliadins even after harsh denaturation, but our results indicate alpha-gliadins ar...

journal_title：Protein and peptide letters

authors： Turner JB,Garner GV,Gordon DB,Brookes SJ,Smith CA

更新日期：2002-02-01 00:00:00