Abstract:
OBJECTIVES:In an attempt to find a more sensitive and specific non-invasive diagnostic assay for the detection of bladder cancer cells, the authors assayed the exfoliated cells from patient's voided urine and bladder washing fluids for the presence of telomerase, an enzyme that maintains a cell's chromosomal length, metalloproteinase-9 (MMP-9), which has been associated with tumor cell invasion and metastasis. Their results were compared with both voided urine cytology (VUC) and bladder wash cytology (BWC) for the detection of bladder cancer cells. MATERIALS AND METHODS:The authors used preoperative voided morning urine samples from 110 subjects for telomerase, matrix metalloproteinase-9 (MMP-9) and cytology. Bladder wash samples were obtained for telomerase and cytology. Of 110 cases 73 were histologically diagnosed as bladder cancer, whereas the remaining 16 had benign urological disorders. A group of 21 healthy volunteers were also enrolled in this study. Cystoscopy was done for all patients as the reference standard for the identification of bladder cancer. Biopsy of any suspicious lesion was performed for histopathological examination. RESULTS:Receiver-operator characteristics (ROC) curves were used to determine the optimal threshold values for telomerase activity in urine, bladder wash and MMP-9 [0.05, 0.088 and 0.51 (ng/ml), respectively]. The levels and the positivity rates of telomerase activity and MMP-9 were significantly higher in the malignant group compared to either the benign group or normal controls. Bladder cancer patients with positive cytology revealed positive telomerase activity in urine, bladder wash, and MMP-9 in 92%, 87% and 61%, respectively. Also, these positive rates were significantly higher in bilharzial bladder cancer cases (88%, 89%, 69%, respectively) compared to non-bilharzial cases (50%, 62.5%, 50%). The overall sensitivity and specificity were 83% and 88.6%, 86.3% and 78.3% for telomerase activity in urine, and in bladder wash, respectively. 66.6% and 80% for MMP-9, 58.5% and 100% for voided urine cytology and 64.4% and 100% for bladder wash cytology. Combined sensitivity of VUC with the 2 biomarkers together was higher than either combined sensitivity of VUC with one of the biomarkers or than that of the biomarker alone. CONCLUSION:Urinary telomerase and MMP-9 had superior sensitivities over VUC; moreover, combined use of these markers increased the sensitivity of cytology from 58.46% to 95%. The higher sensitivities of markers in bilharzial bladder cancer than non-bilharzial type highlight their clinical utility in screening patients with urinary bilharziasis.
journal_name
Anticancer Resjournal_title
Anticancer researchauthors
Eissa S,Swellam M,el-Mosallamy H,Mourad MS,Hamdy N,Kamel K,Zaglol AS,Khafagy MM,el-Ahmady Osubject
Has Abstractpub_date
2003-09-01 00:00:00pages
4347-55issue
5beissn
0250-7005issn
1791-7530journal_volume
23pub_type
杂志文章abstract:BACKGROUND:The major side-effects of bevacizumab in glioma treatment are venous thromboembolic events (VTE). We retrospectively evaluated factors potentially predictive of thromboembolic events. PATIENTS AND METHODS:Bevacizumab, alone or in combination with chemotherapy was used as salvage therapy for recurrence in ma...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:2013-05-01 00:00:00
abstract:BACKGROUND:Dihydroceramide desaturase 1 (DES) is the enzyme responsible for converting dihydroceramide into ceramide in the de novo sphingolipid biosynthesis pathway. Dihydroceramide can inhibit ceramide channel formation to interfere with apoptosis. We have shown that following ceramide synthase knockdown, photodynami...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:2013-01-01 00:00:00
abstract::Germ line point mutations in the RET proto-oncogene have been implicated in four inherited disorders: multiple endocrine neoplasia 2A (MEN 2A) and 2B (MEN 2B); familial medullary thyroid carcinoma (FMTC); and Hirschprung's disease, a congenital lack of enteric plexus neurons. Oncogenically activated RET has also been ...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:1996-09-01 00:00:00
abstract:BACKGROUND:The BCL2 proto-oncogene in non-Hodgkin's lymphoma is a dominant inhibitor of apoptosis. The goal of this work was to develop a (177)Lu-labeled anti-BCL2-peptide nucleic acid (PNA) conjugate designed for dual modality NHL therapy, i.e., simultaneous down-regulation of BCL2-mediated resistance to apoptosis and...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:2011-10-01 00:00:00
abstract::The aim of this study was to investigate the relationship between Technetium-99m (Tc-99m) tetrofosmin accumulation in tumors which may represent the expression of multidrug resistance (MDR)--mediated P-glycoprotein (Pgp) and multidrug resistance-associated protein (MRP) as well as response to chemotherapy in patients ...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:1999-05-01 00:00:00
abstract:BACKGROUND:A nodular lymphocyte predominant Hodgkin's lymphoma (NLPHL) is a lymphoproliferative neoplasm with a fair prognosis, but the possibility of a malignant transformation into a diffuse large B-cell lymphoma (DLBCL) is high. DLBCL progresses aggressively. Introduction of rituximab into therapy had led to improve...
journal_title:Anticancer research
pub_type: 杂志文章
doi:10.21873/anticanres.12865
更新日期:2018-09-01 00:00:00
abstract::The purpose was to evaluate efficacy and safety of carbon ion radiotherapy (C-ion RT) in patients with locally advanced olfactory neuroblastomas (ONBs). This study was a sub-analysis of the Japan Carbon-Ion Radiation Oncology Study Group Study (1402 HN, UMIN000024473). Clinical data of T4 ONBs treated with C-ion RT at...
journal_title:Anticancer research
pub_type: 杂志文章,多中心研究
doi:10.21873/anticanres.12399
更新日期:2018-03-01 00:00:00
abstract::The major purpose of this study was to define if the immunosuppressive effect of a transforming growth factor-beta (TGF-beta)-producing autologous tumor vaccine can be abrogated and rendered immunogenic by suppressing its TGF-beta secretion with antisense strategy. In this study, using a TGF-beta antisense gene modifi...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:2000-05-01 00:00:00
abstract::The association of anaemia and other predictors of fatigue was studied in cancer patients on palliative treatment. A cohort of 128 consecutive patients (61 men and 67 women, mean age 63.6 years; range 36-85) was interviewed using the Edmonton Symptom Assessment System (ESAS) questionnaire, with 11 items describing can...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:2009-07-01 00:00:00
abstract::Bone-seeking radionuclides have been used to treat bone pain due to metastatic bone disease for over 40 years. More than 10 clinical studies using radiostrontium (Sr-89) have shown benefit in about 70-80% of patients having refractory bone pain from prostate, breast and other metastatic bone cancers, with minimal hema...
journal_title:Anticancer research
pub_type: 杂志文章,评审
doi:
更新日期:1988-07-01 00:00:00
abstract:BACKGROUND/AIM:Insulin-like growth factor II mRNA-binding protein 3 (IMP3) plays an important role in the adhesion, invasion, and metastasis of tumor cells. Although emerging evidence suggests that IMP3 promotes tumor progression in several malignancies, the expression of IMP3 and its prognostic implication in adenocar...
journal_title:Anticancer research
pub_type: 杂志文章
doi:10.21873/anticanres.13683
更新日期:2019-09-01 00:00:00
abstract:BACKGROUND:Parathyroid hormone-related protein (PTHrP) is a key regulator of osteolytic metastasis of breast cancer (BC) cells, but its targets and mechanisms of action are not fully understood. This study investigated whether/how PTHrP (1-34) signaling regulates expression of vascular endothelial growth factor (VEGF) ...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:2010-07-01 00:00:00
abstract:UNLABELLED:The aim of the present study was to investigate hypoxia-inducible factor 1α (HIF-1α) and glucose transporter-1 (GLUT-1) expressions as predictors of response and survival after chemoradiotherapy in pretreatment biopsy specimens from patients with rectal cancer. MATERIALS AND METHODS:The immunohistological e...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:2011-05-01 00:00:00
abstract:BACKGROUND/AIM:Rhabdoid tumors (RT) are aggressive pediatric tumors, which show poor prognosis despite use of multimodal intensive therapy. In these tumors, several different oncogenic pathways and epigenetic regulators (like CDK4/6-cyclinD-Rb-signaling, EZH2, histone deacetylases) are contemporaneously deregulated as ...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:2016-08-01 00:00:00
abstract::Over the last two decades, many studies carried out with the aid of lectins have firmly established that cell glycans usually change in the course of the normal processes of growth and development, as well as in pathological situations. We describe here the in vivo binding expression of wheat germ agglutinin (WGA) to ...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:1996-07-01 00:00:00
abstract::K18 is a conjugate of human immunoglobulin G and melphalan and has been reported to accumulate selectively in the tumor. In the present study, the in vitro antitumor effect of K18 was assessed in a total of 107 fresh human tumors by DNA synthesis (3H-thymidine incorporation) inhibition assay, and compared with that of...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:1989-05-01 00:00:00
abstract:BACKGROUND:18F-FDG positron emission tomography (PET) may alter therapeutic management of patients with cervical cancer because 18F-FDG-positive pelvic and para-aortic lymph nodes are associated with lower progression-free survival and vice versa. However, the accuracy of 18F-FDG PET might be impaired by a preceding ly...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:2003-05-01 00:00:00
abstract:AIM:To evaluate the factors predicting for recurrence and to analyze survival of patients with hepatocellular carcinoma (HCC). PATIENTS AND METHODS:A total of 743 patients who were consecutively diagnosed and treated with HCC were retrospectively analyzed from January 2004 to December 2012 at our institution. We analy...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:2013-09-01 00:00:00
abstract::A circadian rhythm of serum concentrations of two tumor markers (CEA and AFP) was demonstrated in individuals not suffering from cancer. These rhythms exhibited a diurnal peak with a nocturnal dip. They disappeared in cancer groups. However, in some cancer subjects, a circadian activity was still detectable, but with ...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:1986-09-01 00:00:00
abstract:BACKGROUND:Oxaliplatin (L-OHP), active in a wide range of human and animal tumours, also CDDP-resistant, possesses unique molecular characteristics of action. However, the mechanisms by which the damage induced by L-OHP triggers a death signal are not yet fully defined. MATERIALS AND METHODS:After L-OHP treatment of t...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:2004-01-01 00:00:00
abstract:BACKGROUND:Live animal studies using an inoculation model of breast cancer indicate that anaesthetic drugs and techniques differentially affect cancer metastasis, inversely related to Natural Killer (NK) cell and T lymphocyte levels. Clinical histological studies demonstrate that the distribution of these immune cells ...
journal_title:Anticancer research
pub_type: 杂志文章,随机对照试验
doi:
更新日期:2015-03-01 00:00:00
abstract:BACKGROUND:A vast majority of patients with metastatic colorectal cancer (mCRC) are not candidates for surgical resection. Radiofrequency ablation (RFA) is a safe and effective technique for treatment of isolated liver metastasis. After radiofrequency ablation, residual tumor can have aggressive growth, part of which i...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:2014-11-01 00:00:00
abstract::This report describes a precise molecular analysis of a rare case of Philadelphia chromosome (Ph) positive acute myeloid leukemia (AML) (FAB classification M2). Phenotypic markers were positive for cells of the myeloid lineage, but negative for B cell and T cell lineage. The leukemic cells carried a Philadelphia chrom...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:1997-01-01 00:00:00
abstract:BACKGROUND/AIM:Dynamics of circulating tumor cells (CTCs) after molecular targeting therapy remain unclear. MATERIALS AND METHODS:We examined changes in CTC numbers and morphology early after targeting therapy in EGFR-mutated PC-9 human lung cancer and HER2-gene amplified GLM-1 gastric cancer mouse CTC models using a ...
journal_title:Anticancer research
pub_type: 杂志文章
doi:10.21873/anticanres.13653
更新日期:2019-09-01 00:00:00
abstract::Combined treatment of interleukin-2 (IL-2), 5-fluorouracil (5-FU) and mitomycin C (MMC) is reported to be effective against hepatic metastasis of colon carcinoma. To clarify this modality, we investigated lymphokine activated killer (LAK) activity of human peripheral lymphocytes on colon cancer cell lines by 51Cr rele...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:1999-01-01 00:00:00
abstract:BACKGROUND/AIM:Hepatitis B virus-encoded X protein (HBx) plays a pivotal role in hepatocellular carcinoma (HCC) progression and treatment resistance. Interestingly, our previous study unexpectedly showed that full-length HBx sensitized HCC cells to lapatinib by up-regulating erb-b2 receptor tyrosine kinase 3 (ERBB3). W...
journal_title:Anticancer research
pub_type: 杂志文章
doi:10.21873/anticanres.13168
更新日期:2019-02-01 00:00:00
abstract::A paclitaxel derivative was used for the preparation of a drug immunoconjugate for the specific targeting to ovarian cancer cells by the BCM43/2E5 monoclonal antibody. To determine the efficiency of conjugation we developed two reproducible immunoassays. Paclitaxel was detected on the conjugate by a paclitaxel immunoa...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:2001-03-01 00:00:00
abstract::Yoshida tumors implanted subcutaneously in the rat are conspicuously heterogeneous. A definite zoning can be discerned. Cell nuclei range from uniform epitheloid (type I) via pleomorphic (type II) to compact (type III). Type III is seen to detach at the rim of the tumor and to infiltrate the dermis. Type I is characte...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:1985-05-01 00:00:00
abstract::A 70-year-old man with a growing liver tumor had undergone subtotal gastrectomy with pancreaticoduodenectomy for gastric cancer (T4b P0 H0 N1, Stage IIIB) 30 months before admission to our hospital. Enhanced computed tomography revealed two hypervascular nodules in segments 4 and 8. After histological diagnosis of sma...
journal_title:Anticancer research
pub_type: 杂志文章
doi:10.21873/anticanres.12389
更新日期:2018-03-01 00:00:00
abstract::We studied a consecutive series of 54 cases of lower lip squamous cell carcinoma (LLSCC) in order to identify any variables which might predict the development of lymph node metastases. The cases were divided into 38 tumors without metastases (group I) and 16 tumors with lymph node metastases (group II). The following...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:1998-03-01 00:00:00