Dynamics of Circulating Tumor Cells Early After Targeting Therapy to Human EGFR-mutated Lung Cancers and HER2 Gene-amplified Gastric Cancers in Mice.

Abstract:

BACKGROUND/AIM:Dynamics of circulating tumor cells (CTCs) after molecular targeting therapy remain unclear. MATERIALS AND METHODS:We examined changes in CTC numbers and morphology early after targeting therapy in EGFR-mutated PC-9 human lung cancer and HER2-gene amplified GLM-1 gastric cancer mouse CTC models using a cytology-based semi-automated CTC detection platform. RESULTS:Erlotinib and T-DM1 inhibited cell growth mainly by induction of apoptosis in vitro. The number of CTCs detected 5-10 days after targeting therapy in mice was significantly increased compared to CTC numbers before therapy. The increased CTCs after therapy consisted of apoptotic CTCs and viable CTCs. This heterogeneous population of CTCs reflects well the cell population of the primary tumor disrupted by therapy. CONCLUSION:CTCs can be mobilized from the primary tumor due to tissue disruption in acute response to targeting therapy, suggesting potential usefulness of CTC monitoring as a predictor of therapeutic response in the clinical settings.

journal_name

Anticancer Res

journal_title

Anticancer research

authors

Ito A,Nakanishi H,Yoshimura M,Ito S,Sakao Y,Kodera Y,Yatabe Y,Kaneda N

doi

10.21873/anticanres.13653

subject

Has Abstract

pub_date

2019-09-01 00:00:00

pages

4711-4720

issue

9

eissn

0250-7005

issn

1791-7530

pii

39/9/4711

journal_volume

39

pub_type

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