Abstract:
:Mouse hepatitis virus (MHV) nucleocapsid (N) protein binds to the large, single-stranded, positive-sense viral genomic RNA to form a helical nucleocapsid structure in mature virions. In addition N protein binds the intracellular form of the genomic RNA, all of the MHV subgenomic mRNAs, and expressed non-MHV RNA transcripts to form ribonucleoprotein (RNP) complexes in infected cells. Among the intracellular viral RNP complexes, only the genomic RNP complex is packaged into virus particles. The present study demonstrated that N protein in the MHV virion nucleocapsid and in the intracellular genome-length RNP complex that bound to viral envelope M protein was tightly self-associated such that its association was retained even after extensive RNase A-treatment of the RNP complexes. The RNase A-resistant tight N protein association in the virion nucleocapsid was not mediated by an intermolecular disulfide bridge between N proteins. In contrast, N protein association in the majority of the intracellular RNP complexes was susceptible to RNase A-treatment. Because the RNP complexes that specifically interact with the M protein are selectively packaged into MHV particles, the present data suggested that there was a distinct difference between N protein association in viral genomic RNP complexes that undergo packaging into virus particles and the subgenomic RNP complexes that are not packaged into MHV particles.
journal_name
Virus Resjournal_title
Virus researchauthors
Narayanan K,Kim KH,Makino Sdoi
10.1016/j.virusres.2003.08.021subject
Has Abstractpub_date
2003-12-01 00:00:00pages
131-40issue
2eissn
0168-1702issn
1872-7492pii
S0168170203002776journal_volume
98pub_type
杂志文章相关文献
VIRUS RESEARCH文献大全abstract::It was previously reported that during serial passage of a BHK-derived cell line persistently infected with foot-and-mouth disease virus, (termed C1-BHK-Rc1) the virus became increasingly more virulent for BHK-21 cells (de la Torre et al 1988). Virus strains isolated from different cell passage levels were tested for ...
journal_title:Virus research
pub_type: 杂志文章
doi:10.1016/0168-1702(90)90084-o
更新日期:1990-12-01 00:00:00
abstract::Cucurbit chlorotic yellows virus (CCYV) is a new member of the genus Crinivirus (family Closteroviridae) with a bi-partite genome. CCYV RNA 1-encoded p22 has recently been reported to be a weak local suppressor of RNA silencing for which an interaction with cucumber SKP1LB1 through an F-box-like motif was demonstrated...
journal_title:Virus research
pub_type: 杂志文章
doi:10.1016/j.virusres.2020.197887
更新日期:2020-04-02 00:00:00
abstract::Between 2006 and 2009, 10 field experiments were done at Kununurra, Carnarvon or Medina in Western Australia (WA) which have tropical, sub-tropical and Mediterranean climates, respectively. These experiments investigated the effectiveness of cultural control measures in limiting ZYMV spread in pumpkin, and single-gene...
journal_title:Virus research
pub_type: 杂志文章
doi:10.1016/j.virusres.2011.04.015
更新日期:2011-08-01 00:00:00
abstract::Herpes simplex virus type 1 (HSV-1) establishes a lifelong latency in the neurons of its host. Sporadically, the latent virus reactivates and spreads back to the original site of infection and causes recrudescent diseases. The only gene actively transcribed during neuronal latency is the latency associated transcript ...
journal_title:Virus research
pub_type: 杂志文章
doi:10.1016/j.virusres.2004.08.011
更新日期:2005-03-01 00:00:00
abstract:BACKGROUND AND AIM:The mechanisms of hepatocarcinogenesis induced by hepatitis C virus remain unclear. Our aim was to investigate the effect of the HCV core protein on the promoter methylation status of selected genes potentially involved in the hepatocellular carcinoma (HCC). MATERIALS AND METHODS:We evaluated the pr...
journal_title:Virus research
pub_type: 杂志文章
doi:10.1016/j.virusres.2011.05.014
更新日期:2011-09-01 00:00:00
abstract::The rabies viruses that circulate in Arctic countries and in much of northern and central Asia are phylogenetically closely related and collectively referred to as the Arctic/Arctic-like (AL) lineage. The emergence and spread of this lineage is of significant interest given that rabies remains a serious zoonotic disea...
journal_title:Virus research
pub_type: 杂志文章
doi:10.1016/j.virusres.2011.10.026
更新日期:2012-01-01 00:00:00
abstract::During 2017, a G4P[6] group A rotavirus strain was identified in the feces of an Italian child hospitalized for acute gastroenteritis in Southern Italy. Nucleotide sequencing of the 11 genomic segments, revealed the G4-P[6]-I1-R1-C1-M1-A1-N1-T1-E1-H1 genotype constellation. Phylogenetic analyses of the gene segments i...
journal_title:Virus research
pub_type: 杂志文章
doi:10.1016/j.virusres.2018.11.003
更新日期:2019-01-15 00:00:00
abstract::Human torovirus (HTV) and Breda virus (BRV), members of the genus torovirus in the family Coronaviridae, are established infectious agents of humans and cattle, respectively. The hemagglutinin-esterase (HE) gene of Breda virus serotype 2 (BRV-2) has been identified and the nucleotide sequence for BRV serotype 1 (BRV-1...
journal_title:Virus research
pub_type: 杂志文章,收录出版
doi:10.1016/s0168-1702(99)00088-x
更新日期:1999-11-01 00:00:00
abstract::Human respiratory syncytial virus (HRSV) P protein, 241 amino acid long, is a structural homotetrameric phosphoprotein. Viral transcription and replication processes are dependent on functional P protein interactions inside viral ribonucleoprotein complexes (RNPs). Binding capacity to RNPs proteins and transcription a...
journal_title:Virus research
pub_type: 杂志文章
doi:10.1016/j.virusres.2007.11.013
更新日期:2008-03-01 00:00:00
abstract::Adult T cell leukemia/lymphoma (ATL) is a highly aggressive CD4+/CD25+ T-cell malignancy caused by human T cell lymphotropic virus type 1 (HTLV-1). Previous studies in the MET-1 cell/NOD/SCID mouse model of ATL demonstrated that MET-1 cells are very susceptible to measles virus (MV) oncolytic therapy. To further evalu...
journal_title:Virus research
pub_type: 杂志文章
doi:10.1016/j.virusres.2014.05.025
更新日期:2014-08-30 00:00:00
abstract::Felid herpesvirus 1 (FHV-1) mutants were constructed using two-step Red-mediated recombination techniques based on a virulent full-length FHV-1 BAC clone. The individual mutant viruses generated were deficient in glycoprotein C (gC), glycoprotein E (gE), US3 serine/threonine protein kinase (PK), or both gE and thymidi...
journal_title:Virus research
pub_type: 杂志文章
doi:10.1016/j.virusres.2016.05.001
更新日期:2016-08-02 00:00:00
abstract::Ribosomal frameshifting is a mechanism of gene expression used by several RNA viruses to express replicase enzymes. This article focuses on frameshifting in two human pathogens, the retrovirus human immunodeficiency virus type 1 (HIV-1) and the coronavirus responsible for severe acute respiratory syndrome (SARS). The ...
journal_title:Virus research
pub_type: 杂志文章,评审
doi:10.1016/j.virusres.2005.10.008
更新日期:2006-07-01 00:00:00
abstract::Over the past three decades diseases caused by whitefly-transmitted geminiviruses (begomoviruses) have emerged to be important constraints to the production of solanaceous crops, particularly tomato (Solanum lycopersicum) and peppers (Capsicum spp.), in many tropical and subtropical regions of the world. The most stud...
journal_title:Virus research
pub_type: 杂志文章
doi:10.1016/j.virusres.2013.12.026
更新日期:2014-06-24 00:00:00
abstract::The purpose of this study was to generate complete genome sequences of Aravan (ARAV), Khujand (KHUV), Irkut (IRKV) and West Caucasian bat (WCBV) viruses, and to compare them with genomes of other lyssaviruses. We focused on RNA-dependent RNA-polymerase (L) and non-coding regions, because other genes of these viruses h...
journal_title:Virus research
pub_type: 杂志文章
doi:10.1016/j.virusres.2008.04.021
更新日期:2008-09-01 00:00:00
abstract::Reports of human infections with highly pathogenic H5N1 avian influenza viruses in many countries in Asia and Africa with varying case fatality rates highlight the pandemic potential of these viruses. In order to contain a rapidly spreading influenza virus in a pandemic scenario, a vaccine which can induce rapid and r...
journal_title:Virus research
pub_type: 杂志文章
doi:10.1016/j.virusres.2013.09.013
更新日期:2013-12-26 00:00:00
abstract::Tobacco mosaic virus (TMV) is a positive-sense plant RNA virus which has a wide host range and a worldwide distribution. Other than a troublesome pathogen, TMV is regarded as a model system pioneering biologic research for a century. The tomato strain of TMV has been recognized to be a distinct tobamovirus as the toma...
journal_title:Virus research
pub_type: 杂志文章
doi:10.1016/j.virusres.2011.09.006
更新日期:2012-01-01 00:00:00
abstract::A comprehensive phylogenetic study based on the hemagglutinin (H) protein of all known African measles virus (MV) isolates is presented. The study includes 64 new H gene sequences from Ghana. Nigeria and South Africa as well as viruses from Zambia and The Gambia for which only incomplete sequencing data were available...
journal_title:Virus research
pub_type: 杂志文章
doi:10.1016/s0168-1702(99)00072-6
更新日期:1999-07-01 00:00:00
abstract::In alfalfa samples analyzed by hightroughput sequencing, four de novo assembled contigs encoding gene products showing identities to alphapartitiviruses proteins were found based on BlastX analysis. The predicted amino acid (aa) sequences of two contigs presented 99-100% identity to the RNA-dependent RNA polymerase (R...
journal_title:Virus research
pub_type: 杂志文章
doi:10.1016/j.virusres.2019.03.021
更新日期:2019-05-01 00:00:00
abstract::The measles virus induces a life-long immune response associated with antibodies specific for the fusion protein. To map the linear immunodominant recognition sites of the fusion (F) protein of the measles virus, we have reacted a complete set of 108 overlapping pentadecapeptides with purified IgG obtained from donor ...
journal_title:Virus research
pub_type: 杂志文章
doi:10.1016/0168-1702(93)90095-5
更新日期:1993-12-01 00:00:00
abstract::Cytoplasmic RNA granules consist of microscopic agglomerates of mRNAs and proteins and occur when the translation is reversibly and temporally halted (stress granules, SGs) or mRNAs are targeted for decapping (processing bodies, PBs). The induction of RNA granules formation by virus infection is a common feature of ma...
journal_title:Virus research
pub_type: 杂志文章
doi:10.1016/j.virusres.2019.197823
更新日期:2020-01-15 00:00:00
abstract::Transplacental viral infections are dependent upon complex interactions between feto-placental and maternal immune responses and the stage of fetal development at which the infection occurs. Bovine viral diarrhea virus (BVDV) has the ability to cross the placenta and infect the fetus. Infection early in gestation with...
journal_title:Virus research
pub_type: 杂志文章
doi:10.1016/j.virusres.2012.05.018
更新日期:2012-08-01 00:00:00
abstract::Japanese encephalitis is a reproductive disorder caused by Japanese encephalitis virus (JEV) in swine. Previous studies have demonstrated that recombinant adenovirus serotype 5 (Ad5) may be a potential vaccine candidate because it can express JEV envelope epitopes and induce immune responses against JEV. Still, it wil...
journal_title:Virus research
pub_type: 杂志文章
doi:10.1016/j.virusres.2016.05.025
更新日期:2016-08-15 00:00:00
abstract::The coronavirus disease 2019 (COVID-19) pandemic has created an urgent need for therapeutics that inhibit the SARS-COV-2 virus and suppress the fulminant inflammation characteristic of advanced illness. Here, we describe the anti-COVID-19 potential of PTC299, an orally bioavailable compound that is a potent inhibitor ...
journal_title:Virus research
pub_type: 杂志文章
doi:10.1016/j.virusres.2020.198246
更新日期:2021-01-15 00:00:00
abstract::Plant viruses express RNA silencing suppressor (RSS) proteins to counteract plant defence mechanisms. Here, we describe a method to assess the RSS activity based on an alfalfa mosaic virus (AMV) RNA 3 expression vector and transgenic Nicotiana tabacum plants that express the P1 and P2 subunits of the AMV replicase (P1...
journal_title:Virus research
pub_type: 杂志文章
doi:10.1016/j.virusres.2019.197733
更新日期:2019-10-15 00:00:00
abstract::A cloned cDNA (1.65 kb) containing the complete glycoprotein gene of the Josiah strain of Lassa virus was inserted into the thymidine kinase (TK) gene of the New York Board of Health (WYETH) strain of vaccinia virus. The Lassa virus glycoprotein precursor, GPC, and the posttranslational cleavage products, G1 and G2, w...
journal_title:Virus research
pub_type: 杂志文章
doi:10.1016/0168-1702(88)90033-0
更新日期:1988-02-01 00:00:00
abstract::This review describes the characterization of porcine circovirus type 2 (PCV2), the field situation of porcine circovirus-associated disease (PCVAD) and the PCV2 vaccine in Korea. PCVAD has been considered the most devastating disease in Korean livestock history since its first outbreak in 1999. Postweaning multisyste...
journal_title:Virus research
pub_type: 杂志文章,评审
doi:10.1016/j.virusres.2011.10.013
更新日期:2012-03-01 00:00:00
abstract::The ultrastructure of the equine morbillivirus (EMV) which was implicated in the death of one human and fourteen horses in Queensland, Australia during September 1994 and a 36 year old man from Queensland in October 1995 is described. The ultrastructure of the virus and the intracellular virus-specific structures are ...
journal_title:Virus research
pub_type: 杂志文章
doi:10.1016/0168-1702(96)01307-x
更新日期:1996-07-01 00:00:00
abstract::A sero-epidemiologic survey of 74 IVDUs and 86 age-matched controls was performed to investigate the prevalence, clinical significance, and genetic distribution of GB virus C (GBV-C)/hepatitis G virus (HGV) infection among intravenous drug users (IVDUs) in Japan. GBV-C/HGV RNA was detected by reverse transcriptase-hem...
journal_title:Virus research
pub_type: 杂志文章
doi:10.1016/s0168-1702(97)01470-6
更新日期:1997-06-01 00:00:00
abstract::Post-translational modification of target proteins by the Small Ubiquitin-like Modifier (SUMO) plays a critical role in regulation of many cellular processes including transcription, RNA processing, protein trafficking, DNA repair, and chromosome segregation, and is also often hijacked by viral infections. Epstein-Bar...
journal_title:Virus research
pub_type: 杂志文章,评审
doi:10.1016/j.virusres.2020.197962
更新日期:2020-07-02 00:00:00
abstract::Crimean-Congo haemorrhagic fever virus (CCHFv) is a member of the genus Nairovirus in the family Bunyaviridae. It possesses a tripartite, single stranded RNA genome of negative polarity consisting of large (L), medium (M) and small (S) segments. CCHF virus is enzootic in life stock and wild animals in many parts of th...
journal_title:Virus research
pub_type: 杂志文章
doi:10.1016/j.virusres.2003.12.035
更新日期:2004-06-15 00:00:00