APOA1 related amyloidosis: a case report and literature review.

abstract：OBJECTIVES:To investigate the possible association between functional variant Ala-9Val in the MnSOD gene and asthma in the case-control study comprising 626 Caucasian subjects. METHODS:MnSOD genotypes were determined by PCR with subsequent restriction analysis by the BsaWI enzyme. RESULTS:Significant differences in a...

journal_title：Clinical biochemistry

authors： Holla LI,Kankova K,Vasku A

更新日期：2006-03-01 00:00:00

abstract：:We describe a fast and efficient method for the isolation of drugs from blood serum by use of the DU PONT PREP I Automated Sample Processor and octadecyl bonded phase columns. Drugs are selectively adsorbed onto C18 bonded phase and eluted with methanol. The recovered residue is essentially free of sample matrix chrom...

journal_title：Clinical biochemistry

authors： Repique EV,Sacks HJ,Farber SJ

更新日期：1981-08-01 00:00:00

abstract：:Improving long-term management, life-quality and outcome of transplant recipients continues to be a challenge. The impact of highly chemoattractant proteins as chemokines on transplant outcome is undeniable. These immunoactive molecules are critical in a wide variety of physiological and pathological processes, such a...

journal_title：Clinical biochemistry

authors： Crescioli C

更新日期：2016-03-01 00:00:00

abstract：:Increasing interest in the use of tumor markers in the clinical management of cancer patients has encouraged development of guidelines by local, national and international groups. Such guidelines generally include recommendations about which markers are likely to be most helpful in given circumstances. Particular requ...

journal_title：Clinical biochemistry

authors： Sturgeon CM

更新日期：2001-07-01 00:00:00

abstract：BACKGROUND:Exercise is a prone condition to enhanced oxidative stress and damage and the specific activity pattern of a soccer match may favour additional pro-oxidant redox alterations. To date, no studies have reported the impact of a soccer match on oxidative stress and muscle damage markers. AIM:To analyse the effe...

journal_title：Clinical biochemistry

authors： Ascensão A,Rebelo A,Oliveira E,Marques F,Pereira L,Magalhães J

更新日期：2008-07-01 00:00:00

abstract：OBJECTIVES:We aimed to investigate the association between methylenetetrahydrofolate reductase (MTHFR) C677T and endothelial nitric oxide synthase (eNOS) G894T polymorphisms with lipid peroxidation, total antioxidant capacity (TAC) and the risk of preeclampsia in preeclamptic women. DESIGN AND METHODS:We screened a sa...

journal_title：Clinical biochemistry

authors： Rahimi Z,Malek-Khosravi S,Rahimi Z,Jalilvand F,Parsian A

更新日期：2013-01-01 00:00:00

abstract：:The use of point-of-care (POC) immunoassays has increased significantly and the menu of analytes continues to expand. Most of the rapid immunoassays are currently based on simple manual assay devices such as the immunochromatographic, agglutination, and immunofiltration assays. Although automated readers have recently...

journal_title：Clinical biochemistry

authors： von Lode P

更新日期：2005-07-01 00:00:00

abstract：OBJECTIVES:An increase in oxidative stress may contribute to the development of oxidative protein damage in the aging rat skeletal muscle. Our aim was to reveal protein carbonyl (PCO), advanced oxidation protein products (AOPP), a novel marker of oxidative stress, and protein thiol (P-SH) levels as markers of protein o...

journal_title：Clinical biochemistry

authors： Cakatay U,Telci A,Kayali R,Tekeli F,Akçay T,Sivas A

更新日期：2003-02-01 00:00:00

abstract：OBJECTIVE:The relationship between the severity of atherosclerotic coronary artery disease (CAD) and circulating levels of salusin-α, salusin-β and heregulin-β1 has been investigated. In addition, the relationship with these peptides and high sensitive C-reactive protein (hsCRP) has been investigated. METHODS:The stud...

journal_title：Clinical biochemistry

authors： Arkan A,Atukeren P,Ikitimur B,Simsek G,Koksal S,Gelisgen R,Ongen Z,Uzun H

更新日期：2021-01-01 00:00:00

abstract：UNLABELLED:Antioxidant capacity testing is commonly used in clinical investigations to provide an estimate of in vitro antioxidant capacity of biosamples. Although beneficial to measure the synergistic contribution of all compounds with antioxidant functionality, assessing the capacity of non-protein fractions or small...

journal_title：Clinical biochemistry

authors： Crowe KM

更新日期：2014-10-01 00:00:00

abstract：:A commercially available assay (Sigma) for urinary oxalates based on the oxalate oxidase/peroxidase catalyzed reaction produced, in our hands, recoveries of added oxalate that varied from patient to patient. A modification of the assay is reported that involves the chromatographic separation of interfering substances,...

journal_title：Clinical biochemistry

authors： Mazzuchin A,Michelutti L,Falter H

更新日期：1990-04-01 00:00:00

abstract：:Retrospective analysis of DNA from paraffin-embedded fixed bone marrow biopsy specimens is possible if preceded by amplification of the DNA sequences of interest by the polymerase chain reaction (PCR). These fixed specimens yield degraded DNA that may not be suitable for direct analysis by conventional digestion and h...

journal_title：Clinical biochemistry

authors： Crisan D,Mattson JC

更新日期：1992-04-01 00:00:00

abstract：:Iodine deficiency (ID) is associated with increased prevalence of goiter, increased risk for neurodevelopmental disorders, and is the world's leading cause of intellectual deficits. Iodine nutritional status of a population is assessed by measurements of urinary iodine concentrations which are also used to define, ind...

journal_title：Clinical biochemistry

authors： Soldin OP

更新日期：2002-11-01 00:00:00

abstract：OBJECTIVES:The change in DNA methylation patterns can be used to distinguish between normal and cancer cells. The aim of the present study was to examine the 5' CpG island methylation patterns of the cancer-testis antigen (CT antigen) gene family, MAGE-As, in hepatocellular carcinoma (HCC), and to develop the DNA demet...

journal_title：Clinical biochemistry

authors： Qiu G,Fang J,He Y

更新日期：2006-03-01 00:00:00

abstract：OBJECTIVES:Studies from both experimental animals and humans suggest that administration of exogenous DHEA may have beneficial endocrine-metabolic, immunologic and neurologic effects. Several groups have administered DHEA to humans, but to the best of our knowledge, no one at this point has published a summary of the r...

journal_title：Clinical biochemistry

authors： Tummala S,Svec F

更新日期：1999-07-01 00:00:00

abstract：OBJECTIVES:This study aims to report a hitherto un-described α-globin gene triplicated allele with a novel hemoglobin (Hb) variant on α2-globin gene, Hb St.Luke's-Thailand [α95(G2)Pro-Arg]. DESIGNS AND METHODS:A study was done on a 1.5-year-old Thai boy who showed pallor and jaundice after birth. Hb analysis was perfo...

journal_title：Clinical biochemistry

authors： Singha K,Fucharoen G,Jetsrisuparb A,Fucharoen S

更新日期：2013-05-01 00:00:00

abstract：OBJECTIVES:Oxidative stress is considered to be a unifying link between diabetes mellitus (DM) and its complications including nephropathy. The aim of the present study was to evaluate oxidative stress status in Asian Indian patients of type 2 DM with nephropathy. DESIGN AND METHODS:Serum levels of malondialdehyde (MD...

journal_title：Clinical biochemistry

authors： Bhatia S,Shukla R,Venkata Madhu S,Kaur Gambhir J,Madhava Prabhu K

更新日期：2003-10-01 00:00:00

abstract：OBJECTIVES:To examine the diagnostic validity of bone alkaline phosphatase (BAP) in serum (a marker of bone formation) and of urinary excretion of pyridinium cross-links (PYR) (a marker of bone resorption) for detection of bone metastases. DESIGN AND METHODS:Two heterogeneous immunoassays were employed for determinati...

journal_title：Clinical biochemistry

authors： Withold W,Georgescu G,Khakzad H,Vosberg H,Mueller-Gaertner HW,Reinauer H

更新日期：1995-10-01 00:00:00

abstract：OBJECTIVES:This study investigates how some of human physiological parameters, in particular sex and daytime, can contribute to the normal variation in blood gene expression. DESIGN AND METHODS:The expressions of two genes: SOD1 (Cu/Zn) and SOD2 (Mn) in white blood cells of 19 healthy individuals (10 men and 9 women) ...

journal_title：Clinical biochemistry

authors： Khymenets O,Covas MI,Farré M,Langohr K,Fitó M,de la Torre R

更新日期：2008-11-01 00:00:00

abstract：:1. Six subjects were investigated during the luteal phase of the menstrual cycle to determine whether the ovarian steroids, estrogen and progesterone were released in an episodic manner. 2. Analysis of plasma obtained every 20 min. during a normal working day provided no evidence for pulsatile release of either steroi...

journal_title：Clinical biochemistry

authors： Younglai EV,Smith SL,Cleghorn JM,Streiner DL

更新日期：1975-08-01 00:00:00

abstract：OBJECTIVES:Current extraction methods for cobalamins from serum influence the molecular characteristics of the vitamin. Therefore, an extraction procedure that leaves the cobalamins unchanged is needed. DESIGN AND METHODS:Monoclonal antibodies towards transcobalamin (TC) and haptocorrin (HC) (in house) linked to magne...

journal_title：Clinical biochemistry

authors： Hardlei TF,Morkbak AL,Nexo E

更新日期：2007-12-01 00:00:00

abstract：OBJECTIVE:Previous studies suggested that decreased serum vaspin levels were associated with coronary artery disease (CAD). The present study aimed to investigate the association between plasma vaspin levels and different states of CAD. DESIGN AND METHODS:A total of 162 patients with coronary angiography (CAG) proved ...

journal_title：Clinical biochemistry

authors： Zhang B,Peng W,Li H,Lu Y,Zhuang J,Wang K,Su Y,Xu Y

更新日期：2013-10-01 00:00:00

abstract：:The ES 300 (Boehringer Mannheim Diagnostics, Indianapolis, IN) is a new automated immunoassay analyzer intended for the quantitative determination of a wide range of analytes. We compared its performance to enzyme immunoassay (EIA), fluorescence polarization immunoassay (FPIA), and radioimmunoassay (RIA) methods for c...

journal_title：Clinical biochemistry

authors： Camara PD,Velletri K,Krupski M,Rosner M,Griffiths WC

更新日期：1992-08-01 00:00:00

abstract：OBJECTIVES:The aim of the present study was to establish the range of chitotriosidase (CT) activity in normal individuals, patients with Gaucher disease (GD) and Niemann-Pick disease (NPD), types A or B. The kinetics of CT in these three groups was also investigated. DESIGN AND METHODS:CT activity, as well as Km, Vmax...

journal_title：Clinical biochemistry

authors： Wajner A,Michelin K,Burin MG,Pires RF,Pereira ML,Giugliani R,Coelho JC

更新日期：2004-10-01 00:00:00

abstract：OBJECTIVES:The aim of the study was to evaluate the concentration of interleukin 12 (IL-12), the activity of phospholipase A(2) (PLA(2)), and platelet-activating factor acetylhydrolase (PAF-AH) in type 1 diabetes (DM1) patients treated with intensive insulin therapy. DESIGN AND METHODS:Studied parameters were measured...

journal_title：Clinical biochemistry

authors： Wegner M,Araszkiewicz A,Pioruńska-Mikołajczak A,Zozulińska-Ziółkiewicz D,Wierusz-Wysocka B,Pioruńska-Stolzmann M

更新日期：2009-11-01 00:00:00

abstract：OBJECTIVE:This article will review the advancements and new developments being made in (1) advanced computers, (2) microtechnology, (3) advanced immunodiagnostics, (4) neural networks, and (5) molecular biology. The influence of these technologies and their products on clinical laboratories is also discussed. CONCLUSI...

journal_title：Clinical biochemistry

authors： Burtis CA

更新日期：1995-06-01 00:00:00

abstract：OBJECTIVES:To verify the role of IL-12 in the pathogenesis of psoriasis and determine its relation to IFNgamma. DESIGN AND METHODS:Skin biopsies from lesional and non-lesional skin of 30 patients and 10 healthy controls were obtained for quantitative PCR examination of IL-12 (P40) and IFNgamma mRNA as well as in situ ...

journal_title：Clinical biochemistry

authors： Shaker OG,Moustafa W,Essmat S,Abdel-Halim M,El-Komy M

更新日期：2006-02-01 00:00:00

abstract：OBJECTIVE:Develop a simple LC-MS-MS method for determination of 25-hydroxymetabolites of vitamin D2 and D3 in serum. DESIGN AND METHODS:0.1 mL serum was mixed with 0.3 mL deproteinizing mixture containing two deuterated internal standards. Two LC-MS-MS systems were used: Waters Premier with an ESI source and a Hypersi...

journal_title：Clinical biochemistry

authors： Bogusz MJ,Al Enazi E,Tahtamoni M,Jawaad JA,Al Tufail M

更新日期：2011-11-01 00:00:00

abstract：OBJECTIVES:Angiotensin converting enzyme (ACE), G-Protein couple receptor (G-Prot), endothelial nitric oxide synthase (ecNOS), Leptin -2548G/A and uncoupling protein (UCP2) are potent regulators of intra renal hemodynamics and may be the causative factors contributing to the deterioration of renal functions. In recent ...

journal_title：Clinical biochemistry

authors： Tripathi G,Sharma RK,Baburaj VP,Sankhwar SN,Jafar T,Agrawal S

更新日期：2008-05-01 00:00:00

abstract：OBJECTIVE:We used single-strand conformational polymorphism (SSCP). To screen for mutations/polymorphisms in exon 4 of the apolipoprotein C III in 45 patients with hypertriglyceridemia and 46 control individuals, single-strand conformational polymorphism was investigated using restriction endonuclease and amplification...

journal_title：Clinical biochemistry

authors： Beheshti I,Hanson NQ,Copeland KR,Garg U,Tsai MY

更新日期：1995-06-01 00:00:00