Abstract:
:Cells with endothelial phenotype generated from adult peripheral blood have emerging diagnostic and therapeutic potential. This study examined the lineage relationship between, and angiogenic function of, early endothelial progenitor cells (EPCs) and late outgrowth endothelial cells (OECs) in culture. Culture conditions were established to support the generation of both EPCs and OECs from the same starting population of peripheral blood mononuclear cells (PBMCs). Utilizing differences in expression of the surface endotoxin receptor CD14, it was determined that the vast majority of EPCs arose from a CD14+ subpopulation of PBMCs but OECs developed exclusively from the CD14- fraction. Human OECs, but not EPCs, expressed key regulatory proteins endothelial nitric oxide synthase (eNOS) and caveolin-1. Moreover, OECs exhibited a markedly greater capacity for capillary morphogenesis in in vitro and in vivo matrigel models, tube formation by OECs being in part dependent on eNOS function. Collectively, these data indicate lineage and functional heterogeneity in the population of circulating cells capable of assuming an endothelial phenotype and provide rationale for the investigation of new cell-therapeutic approaches to ischemic cardiovascular disease.
journal_name
Circ Resjournal_title
Circulation researchauthors
Gulati R,Jevremovic D,Peterson TE,Chatterjee S,Shah V,Vile RG,Simari RDdoi
10.1161/01.RES.0000105569.77539.21subject
Has Abstractpub_date
2003-11-28 00:00:00pages
1023-5issue
11eissn
0009-7330issn
1524-4571pii
01.RES.0000105569.77539.21journal_volume
93pub_type
杂志文章abstract:RATIONALE:We have recently shown that the bone morphogenetic protein (BMP) antagonist Gremlin 2 (Grem2) is required for early cardiac development and cardiomyocyte differentiation. Our initial studies discovered that Grem2 is strongly induced in the adult heart after experimental myocardial infarction (MI). However, th...
journal_title:Circulation research
pub_type: 杂志文章
doi:10.1161/CIRCRESAHA.116.308700
更新日期:2016-07-22 00:00:00
abstract::Myocardial stunning is a form of reversible myocardial ischemia/reperfusion injury associated with systolic and diastolic contractile dysfunction. In the isolated rat heart model, myocardial stunning is characterized by specific C-terminal proteolysis of the myofilament protein, troponin I (cTnI) that yields cTnI1-193...
journal_title:Circulation research
pub_type: 杂志文章
doi:10.1161/01.RES.0000099889.35340.6F
更新日期:2003-11-14 00:00:00
abstract:RATIONALE:We previously reported that vascular endothelial growth factor (VEGF)-induced binding of VEGF receptor 2 (VEGFR2) to epsins 1 and 2 triggers VEGFR2 degradation and attenuates VEGF signaling. The epsin ubiquitin interacting motif (UIM) was shown to be required for the interaction with VEGFR2. However, the mole...
journal_title:Circulation research
pub_type: 杂志文章
doi:10.1161/CIRCRESAHA.115.307679
更新日期:2016-03-18 00:00:00
abstract::Previously, it was shown that the D enantiomer of isoidide dinitrate was 10-fold more potent than the L enantiomer and 10-fold less potent than glyceryl trinitrate for stimulating cyclic GMP accumulation and relaxation of isolated rat aorta. In the present study, these organic nitrates were tested for their ability to...
journal_title:Circulation research
pub_type: 杂志文章
doi:10.1161/01.res.63.4.693
更新日期:1988-10-01 00:00:00
abstract::Disorders of L-type Ca2+ channels can cause severe cardiac arrhythmias. A subclass of small GTP-binding proteins, the RGK family, regulates L-type Ca2+ current (I(Ca,L)) in heterologous expression systems. Among these proteins, Rad (Ras associated with diabetes) is highly expressed in the heart, although its role in t...
journal_title:Circulation research
pub_type: 杂志文章
doi:10.1161/CIRCRESAHA.106.146399
更新日期:2007-07-06 00:00:00
abstract::This study was designed to determine if opioids were detectable in cerebrospinal fluid (CSF) and if these concentrations were altered by hemorrhagic hypotension. This study was further designed to determine the effects of topically administered opioids on pial arteriolar diameter during normotension and hypotension. C...
journal_title:Circulation research
pub_type: 杂志文章
doi:10.1161/01.res.68.4.922
更新日期:1991-04-01 00:00:00
abstract::Cardiac hemodynamics, diameter, blood flow velocity, and volumic flow of the brachial artery were studied before and after diltiazem administration in 11 patients with sustained essential hypertension. The study was compared with the hemodynamic effects of dihydralazine. The caliber of the brachial artery was evaluate...
journal_title:Circulation research
pub_type: 杂志文章
doi:
更新日期:1983-02-01 00:00:00
abstract::In the mammalian myocardium, potassium (K(+)) channels control resting potentials, action potential waveforms, automaticity, and refractory periods and, in most cardiac cells, multiple types of K(+) channels that subserve these functions are expressed. Molecular cloning has revealed the presence of a large number of K...
journal_title:Circulation research
pub_type: 杂志文章,评审
doi:10.1161/hh2301.100349
更新日期:2001-11-23 00:00:00
abstract:RATIONALE:Complementation of pluripotency genes may improve adult stem cell functions. OBJECTIVES:Here we show that clonally expandable, telomerase expressing progenitor cells can be isolated from peripheral blood of children. The surface marker profile of the clonally expanded cells is distinct from hematopoietic or ...
journal_title:Circulation research
pub_type: 杂志文章
doi:10.1161/CIRCRESAHA.109.206045
更新日期:2010-04-16 00:00:00
abstract:RATIONALE:The heart contains abundant interstitial and perivascular fibroblasts. Traditional views suggest that, under conditions of mechanical stress, cytokines, growth factors, and neurohumoral mediators stimulate fibroblast activation, inducing ECM (extracellular matrix) protein synthesis and promoting fibrosis and ...
journal_title:Circulation research
pub_type: 杂志文章
doi:10.1161/CIRCRESAHA.118.314438
更新日期:2019-04-12 00:00:00
abstract:RATIONALE:Diabetes mellitus increases cardiovascular disease risk in humans and remains elevated despite cholesterol-lowering therapy with statins. Consistent with this, in mouse models, diabetes mellitus impairs atherosclerosis plaque regression after aggressive cholesterol lowering. MicroRNA 33 (miR33) is a key negat...
journal_title:Circulation research
pub_type: 杂志文章
doi:10.1161/CIRCRESAHA.115.304164
更新日期:2014-10-10 00:00:00
abstract:RATIONALE:G protein-coupled receptor kinase 2 (GRK2) is abundantly expressed in the heart, and its expression and activity are increased in injured or stressed myocardium. This upregulation has been shown to be pathological. GRK2 can promote cell death in ischemic myocytes, and its inhibition by a peptide comprising th...
journal_title:Circulation research
pub_type: 杂志文章
doi:10.1161/CIRCRESAHA.112.300754
更新日期:2013-04-12 00:00:00
abstract::Angiotensin II exerts positive inotropic and chronotropic effects on the mammalian heart by binding to specific membrane receptors. Recently, two subtypes of angiotensin II receptors (AT1 and AT2) have been distinguished by using the nonpeptide antagonists losartan (previously known as DuP 753) and PD123177. To evalua...
journal_title:Circulation research
pub_type: 杂志文章
doi:10.1161/01.res.71.6.1482
更新日期:1992-12-01 00:00:00
abstract::Transforming growth factor (TGF)-beta plays a major role in the development of vascular diseases. Despite the pleiotropic effects of TGF-ss on vascular smooth muscle cells (VSMCs), only a few genes have been characterized as direct targets of TGF-beta in VSMCs. Cardiac ankyrin repeat protein (CARP) has been thought to...
journal_title:Circulation research
pub_type: 杂志文章
doi:10.1161/01.res.88.1.30
更新日期:2001-01-19 00:00:00
abstract::AR-L 115BS, a benzimidazole derivative, is a positive inotropic agent that has been shown to increase active tension development and unloaded shortening velocity of chemically skinned heart muscle preparations at submaximal activating levels of free Ca++. We measured the effect of AR-L on relations between free Ca++, ...
journal_title:Circulation research
pub_type: 杂志文章
doi:10.1161/01.res.51.3.290
更新日期:1982-09-01 00:00:00
abstract::Troponin is essential for the regulation of cardiac contraction. Troponin is a sarcomeric molecular switch, directly regulating the contractile event in concert with intracellular calcium signals. Troponin isoform switching, missense mutations, proteolytic cleavage, and posttranslational modifications are known to dir...
journal_title:Circulation research
pub_type: 杂志文章,评审
doi:10.1161/01.RES.0000110083.17024.60
更新日期:2004-02-06 00:00:00
abstract::Na(v)1.5, the cardiac isoform of the voltage-gated Na+ channel, is critical to heart excitability and conduction. However, the mechanisms regulating its expression at the cell membrane are poorly understood. The Na(v)1.5 C-terminus contains a PY-motif (xPPxY) that is known to act as binding site for Nedd4/Nedd4-like u...
journal_title:Circulation research
pub_type: 杂志文章
doi:10.1161/01.RES.0000136816.05109.89
更新日期:2004-08-06 00:00:00
abstract::A number of neurotransmitters modulate cardiac dihydropyridine-sensitive L-type Ca2+ channels through several homologous G protein-coupled receptors. Previous studies that have examined receptor-Ca2+ channel interactions have suffered because of the coexpression of various receptor subtypes in native cells. To study t...
journal_title:Circulation research
pub_type: 杂志文章
doi:10.1161/01.res.76.3.335
更新日期:1995-03-01 00:00:00
abstract::Nitroglycerin dilates large (greater than or equal to 100 microns) but not small coronary arterial microvessels, and a putative metabolite of nitroglycerin, S-nitroso-L-cysteine, has been shown in vitro to dilate both large and small coronary microvessels. Based on this evidence, we tested the hypothesis that the lack...
journal_title:Circulation research
pub_type: 杂志文章
doi:10.1161/01.res.68.3.847
更新日期:1991-03-01 00:00:00
abstract::Bradykinin relaxes arterioles on the brain's surface. This response is endothelium-dependent. The data presented here confirm the hypothesis that hydroxyl free radical mediates this response and may be the endothelium-dependent relaxing factor for bradykinin in this microvascular bed. The response to a locally applied...
journal_title:Circulation research
pub_type: 杂志文章
doi:10.1161/01.res.61.4.601
更新日期:1987-10-01 00:00:00
abstract::Intracellular potassium activity in guinea pig left atria was measured using potassium ion-selective microelectrodes and conventional microelectrodes. The effects of extracellular potassium concentration and acetylcholine on both intracellular potassium activity and the relationship between the resting membrane potent...
journal_title:Circulation research
pub_type: 杂志文章
doi:10.1161/01.res.54.1.65
更新日期:1984-01-01 00:00:00
abstract:RATIONALE:We previously reported that type VI collagen deposition increases in the infarcted myocardium in vivo. To date, a specific role for this nonfibrillar collagen has not been explored in the setting of myocardial infarction (MI). OBJECTIVE:To determine whether deletion of type VI collagen in an in vivo model of...
journal_title:Circulation research
pub_type: 杂志文章
doi:10.1161/CIRCRESAHA.111.252734
更新日期:2012-03-16 00:00:00
abstract::High-density lipoprotein (HDL) is a complex mixture of lipoproteins that is associated with many minor proteins and lipids that influence the function of HDL. Although HDL is a promising marker and potential therapeutic target based on its epidemiological data and the effects of healthy HDL in vitro in endothelial cel...
journal_title:Circulation research
pub_type: 杂志文章,评审
doi:10.1161/CIRCRESAHA.114.300935
更新日期:2014-01-03 00:00:00
abstract::The focal pattern of atherosclerotic lesions in arterial vessels suggests that local blood flow patterns are important factors in atherosclerosis. Although disturbed flows in the branches and curved regions are proatherogenic, laminar flows in the straight parts are atheroprotective. Results from in vitro studies on c...
journal_title:Circulation research
pub_type: 杂志文章,评审
doi:10.1161/01.res.0000038487.19924.18
更新日期:2002-11-01 00:00:00
abstract::In urethane-anesthetized rats, electrophysiological recordings of spontaneously active neurons in the vasopressor area of the rostral ventrolateral medulla were analyzed for participation in cardiovascular regulation. A total of 138 units were found which were inhibited by transient increases in mean arterial pressure...
journal_title:Circulation research
pub_type: 杂志文章
doi:10.1161/01.res.56.3.359
更新日期:1985-03-01 00:00:00
abstract::The cardiac high molecular weight proteins/ryanodine receptors were purified to homogeneity from junctional sarcoplasmic reticulum membranes and shown to exhibit large conductance calcium channel activity. High molecular weight proteins were solubilized from junctional sarcoplasmic reticulum in zwitterionic detergent ...
journal_title:Circulation research
pub_type: 杂志文章
doi:10.1161/01.res.64.4.779
更新日期:1989-04-01 00:00:00
abstract::Increasing evidence indicates that the control of cell proliferation and apoptosis are linked. The c-myc proto-oncogene is induced early after cell-cycle entry in vascular smooth muscle cells (VSMCs) in vitro and after arterial injury and regulates both cell proliferation and apoptosis. Although both proliferation and...
journal_title:Circulation research
pub_type: 杂志文章
doi:10.1161/01.res.84.7.820
更新日期:1999-04-16 00:00:00
abstract::Topical application of sodium arachidonate (50-200 micrograms/ml) or bradykinin (0.1-10 micrograms/ml) on the brain surface of anesthetized cats caused dose-dependent cerebral arteriolar dilation. This dilation was blocked by 67-100% in the presence of superoxide dismutase and catalase. These enzymes did not affect th...
journal_title:Circulation research
pub_type: 杂志文章
doi:10.1161/01.res.55.3.295
更新日期:1984-09-01 00:00:00
abstract::To determine the time course of efferent sympathetic denervation after transmural myocardial infarction (TMI) and of efferent vagal denervation after transmural or nontransmural myocardial infarction (NTMI), we measured effective refractory periods (ERP) basal and apical to TMI or NTMI in secobarbital-anesthetized, op...
journal_title:Circulation research
pub_type: 杂志文章
doi:10.1161/01.res.62.6.1111
更新日期:1988-06-01 00:00:00
abstract:RATIONALE:Clinical benefits of reperfusion after myocardial infarction are offset by maladaptive innate immune cell function, and therapeutic interventions are lacking. OBJECTIVE:We sought to test the significance of phagocytic clearance by resident and recruited phagocytes after myocardial ischemia reperfusion. METH...
journal_title:Circulation research
pub_type: 杂志文章
doi:10.1161/CIRCRESAHA.117.311327
更新日期:2017-09-29 00:00:00