Abstract:
:Angiotensin II exerts positive inotropic and chronotropic effects on the mammalian heart by binding to specific membrane receptors. Recently, two subtypes of angiotensin II receptors (AT1 and AT2) have been distinguished by using the nonpeptide antagonists losartan (previously known as DuP 753) and PD123177. To evaluate the tissue distribution and subtypes of angiotensin II receptors in rat heart, we performed a 125I-[Sar1,Ile8]angiotensin II in situ binding assay on tissue sections obtained from adult Sprague-Dawley rats (10 and 14 weeks old). Binding specificity was verified by competition with unlabeled [Sar1]angiotensin II. Distribution of AT1 and AT2 receptors was determined by competition with losartan and PD123177, respectively, and the density of the receptors was quantified by emulsion autoradiography. Angiotensin II receptors were widely distributed throughout the heart, with each receptor subtype accounting for approximately 50% of the specific binding. Binding density was comparable in the atria, right and left ventricles, intraventricular septum, and sinoatrial node, whereas it was significantly greater in the atrioventricular node. The AT1 receptor appears to interact with guanidine nucleotide regulatory proteins, because GTP-gamma-S causes dissociation of the radioligand from this receptor. In contrast, the AT2 receptor does not appear to directly interact with guanine nucleotide regulatory proteins, inasmuch as radioligand dissociation from this receptor subtype is not affected by GTP-gamma-S. Because angiotensin II has been reported to have growth-potentiating effects in several tissues, we examined angiotensin II receptors in fetal (embryonic days 16 and 19) and neonatal (1-, 2-, 3-, and 10-day-old) rats.(ABSTRACT TRUNCATED AT 250 WORDS)
journal_name
Circ Resjournal_title
Circulation researchauthors
Sechi LA,Griffin CA,Grady EF,Kalinyak JE,Schambelan Mdoi
10.1161/01.res.71.6.1482subject
Has Abstractpub_date
1992-12-01 00:00:00pages
1482-9issue
6eissn
0009-7330issn
1524-4571journal_volume
71pub_type
杂志文章abstract:RATIONALE:Protein kinase (PK)C-induced phosphorylation of cardiac troponin (cTn)I has been shown to regulate cardiac contraction. OBJECTIVE:Characterize functional effects of increased PKC-induced cTnI phosphorylation and identify underlying mechanisms using a transgenic mouse model (cTnI(PKC-P)) expressing mutant cTn...
journal_title:Circulation research
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更新日期:2009-12-04 00:00:00
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pub_type: 杂志文章,评审
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doi:10.1161/CIRCRESAHA.109.204891
更新日期:2010-03-19 00:00:00
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pub_type: 杂志文章
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更新日期:1976-07-01 00:00:00
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pub_type: 杂志文章
doi:10.1161/01.res.65.1.83
更新日期:1989-07-01 00:00:00
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更新日期:1978-05-01 00:00:00
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pub_type: 杂志文章
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更新日期:1975-06-01 00:00:00
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pub_type: 杂志文章
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更新日期:2014-02-28 00:00:00
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pub_type: 杂志文章
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更新日期:1999-11-26 00:00:00
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pub_type: 杂志文章
doi:10.1161/01.res.61.4.560
更新日期:1987-10-01 00:00:00
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pub_type: 杂志文章
doi:10.1161/CIRCRESAHA.118.312804
更新日期:2018-10-26 00:00:00
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pub_type: 杂志文章
doi:10.1161/01.RES.0000191538.76771.66
更新日期:2005-11-25 00:00:00
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pub_type: 杂志文章
doi:10.1161/01.RES.0000136816.05109.89
更新日期:2004-08-06 00:00:00
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journal_title:Circulation research
pub_type: 杂志文章
doi:10.1161/CIRCRESAHA.112.266403
更新日期:2012-06-22 00:00:00
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journal_title:Circulation research
pub_type: 杂志文章
doi:10.1161/01.res.37.6.787
更新日期:1975-12-01 00:00:00
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更新日期:1981-02-01 00:00:00
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更新日期:2001-04-13 00:00:00
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更新日期:2000-10-13 00:00:00
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更新日期:1995-08-01 00:00:00
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pub_type: 杂志文章
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更新日期:2020-12-04 00:00:00
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更新日期:1994-09-01 00:00:00
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pub_type: 杂志文章,评审
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更新日期:1989-08-01 00:00:00
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更新日期:2011-12-09 00:00:00
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更新日期:1980-07-01 00:00:00
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更新日期:2002-10-04 00:00:00