Opioids in cerebrospinal fluid in hypotensive newborn pigs.


:This study was designed to determine if opioids were detectable in cerebrospinal fluid (CSF) and if these concentrations were altered by hemorrhagic hypotension. This study was further designed to determine the effects of topically administered opioids on pial arteriolar diameter during normotension and hypotension. Closed cranial windows were used to determine pial arteriolar diameter. Periarachnoid cortical and cisterna magna CSF was collected from piglets during normotension and hypotension (systemic arterial pressure decreased from 63 +/- 1 to 33 +/- 1 mm Hg). Opioid profiles were assessed qualitatively by radioreceptor assay, and individual opioids were measured quantitatively by radioimmunoassay. Periarachnoid cortical and cisterna magna CSF methionine enkephalin-, leucine enkephalin-, dynorphin-, and beta-endorphin-like receptor active values all were increased by hypotension. When quantified by radioimmunoassay, periarachnoid cortical CSF values for methionine enkephalin-like immunoreactivity were 1,167 +/- 58 and 2,975 +/- 139 pg/ml for normotension and hypotension, respectively. Periarachnoid cortical CSF radioimmunoassay values for dynorphin-like immunoreactivity were 15 +/- 2 and 28 +/- 2 pg/ml for normotension and hypotension, respectively. When applied topically to the cortical surface, synthetic methionine enkephalin increased pial arteriolar diameter (134 +/- 4, 158 +/- 4, and 163 +/- 4 microns for control, 574 pg/ml [10(-10) M], and 5,740 pg/ml [10(-9) M], respectively). Similarly, topical synthetic leucine enkephalin and dynorphin elicited pial arteriolar dilation. However, beta-endorphin produced arteriolar constriction. Hypotension attenuated methionine and leucine enkephalin-induced dilation and reversed dynorphin-induced dilation to concentration-dependent constriction. beta-Endorphin-induced constriction was not changed by hypotension. Therefore, opioids could contribute to the control of the cerebral circulation during hypotension.


Circ Res


Circulation research


Armstead WM,Mirro R,Busija DW,Desiderio DM,Leffler CW




Has Abstract


1991-04-01 00:00:00












  • Molecular and particulate depositions for regional myocardial flows in sheep.

    abstract::The deposition of microspheres in small tissue regions is not strictly flow dependent. In comparison with the soluble flow marker 2-iododesmethylimipramine (IDMI), deposition of 16.5-microns microspheres was mildly but systematically biased into high flow regions of rabbit hearts (Bassingthwaighte JB, Malone MA, Moffe...

    journal_title:Circulation research

    pub_type: 杂志文章


    authors: Bassingthwaighte JB,Malone MA,Moffett TC,King RB,Chan IS,Link JM,Krohn KA

    更新日期:1990-05-01 00:00:00

  • Specific induction of tie1 promoter by disturbed flow in atherosclerosis-prone vascular niches and flow-obstructing pathologies.

    abstract::Nonlaminar flow is a major predisposing factor to atherosclerosis. Yet little is known regarding hemodynamic gene regulation in disease-prone areas of the vascular tree in vivo. We have determined spatial patterns of expression of endothelial cell receptors in the arterial tree and of reporter gene constructs in trans...

    journal_title:Circulation research

    pub_type: 杂志文章


    authors: Porat RM,Grunewald M,Globerman A,Itin A,Barshtein G,Alhonen L,Alitalo K,Keshet E

    更新日期:2004-02-20 00:00:00

  • Aging does not affect the activation of the myocyte insulin-like growth factor-1 autocrine system after infarction and ventricular failure in Fischer 344 rats.

    abstract::To determine whether the attenuation in the growth capacity of myocytes in the overloaded aging heart is associated with an impairment in the activation of insulin-like growth factor-1 (IGF-1) and its receptor (IGF-1R) in the stressed cells, large myocardial infarcts were produced in Fischer 344 rats at 4 and 16 month...

    journal_title:Circulation research

    pub_type: 杂志文章


    authors: Cheng W,Reiss K,Li P,Chun MJ,Kajstura J,Olivetti G,Anversa P

    更新日期:1996-04-01 00:00:00

  • Matrix metalloproteinase-3 genotype contributes to age-related aortic stiffening through modulation of gene and protein expression.

    abstract::Matrix metalloproteinases (MMPs) include most major constituents of the arterial wall as substrates. A common promotor polymorphism (5A/6A) is associated with differences in MMP-3 (stromelysin-1) activity, and associations with certain forms of vascular disease have been shown. This study investigated whether the MMP-...

    journal_title:Circulation research

    pub_type: 杂志文章


    authors: Medley TL,Kingwell BA,Gatzka CD,Pillay P,Cole TJ

    更新日期:2003-06-13 00:00:00

  • Cellular bioenergetics is an important determinant of the molecular imaging signal derived from luciferase and the sodium-iodide symporter.

    abstract:RATIONALE:Molecular imaging is useful for longitudinal assessment of engraftment. However, it is not known which factors, other than cell number, can influence the molecular imaging signal obtained from reporter genes. OBJECTIVE:The effects of cell dissociation/suspension on cellular bioenergetics and the signal obtai...

    journal_title:Circulation research

    pub_type: 杂志文章


    authors: Chang C,Chan A,Lin X,Higuchi T,Terrovitis J,Afzal JM,Rittenbach A,Sun D,Vakrou S,Woldemichael K,O'Rourke B,Wahl R,Pomper M,Tsui B,Abraham MR

    更新日期:2013-02-01 00:00:00

  • Changes in extracellular potassium activity in response to decreased pH in rabbit atrial muscle.

    abstract::The extracellular and intracellular potassium (K+) activities of isolated superfused rabbit atrial muscle were measured using K+-sensitive liquid ion exchanger microelectrodes. When the pH of the bathing medium was decreased from 7.5 to 6.8, intracellular K+ activity fell and extracellular K+ activity rose from a mean...

    journal_title:Circulation research

    pub_type: 杂志文章


    authors: Skinner RB Jr,Kunze DL

    更新日期:1976-11-01 00:00:00

  • Increased sarcoplasmic reticulum calcium leak but unaltered contractility by acute CaMKII overexpression in isolated rabbit cardiac myocytes.

    abstract::The predominant cardiac Ca2+/calmodulin-dependent protein kinase (CaMK) is CaMKIIdelta. Here we acutely overexpress CaMKIIdeltaC using adenovirus-mediated gene transfer in adult rabbit ventricular myocytes. This circumvents confounding adaptive effects in CaMKIIdeltaC transgenic mice. CaMKIIdeltaC protein expression a...

    journal_title:Circulation research

    pub_type: 杂志文章


    authors: Kohlhaas M,Zhang T,Seidler T,Zibrova D,Dybkova N,Steen A,Wagner S,Chen L,Brown JH,Bers DM,Maier LS

    更新日期:2006-02-03 00:00:00

  • Differential effects of digoxin at comparable concentrations in tissues of fetal and adult sheep.

    abstract::Electrocardiogram (ECG) electrodes and carotid arterial and superior vena caval (SVC) catheters were placed in eight nonpregnant ewes and 11 fetuses (109-129 days gestation) to measure heart rate, arterial presssure, P-R interval, left ventricular pre-ejection period (PEP), and left ventricular ejection time (LVET), b...

    journal_title:Circulation research

    pub_type: 杂志文章


    authors: Berman W Jr,Ravenscroft PJ,Sheiner LB,Heymann MA,Melmon KL,Rudolph AM

    更新日期:1977-11-01 00:00:00

  • Distinct behavior of cardiac myosin heavy chain gene constructs in vivo. Discordance with in vitro results.

    abstract::Transcriptional thyroid hormone responsiveness of the cardiac alpha-myosin heavy chain (alpha-MHC) gene has been demonstrated in transfections into fetal and neonatal cardiomyocytes and in transgenic mice. However, the correspondence between the regulation of MHC expression in dissociated cells with that in the intact...

    journal_title:Circulation research

    pub_type: 杂志文章


    authors: Buttrick PM,Kaplan ML,Kitsis RN,Leinwand LA

    更新日期:1993-06-01 00:00:00

  • Disease pathways and novel therapeutic targets in hypertrophic cardiomyopathy.

    abstract::As described in earlier reviews in this series on the molecular basis of hypertrophic cardiomyopathy (HCM), HCM is one of the archetypal monogenic cardiovascular disorders to be understood at the molecular level. Twenty years after the discovery of the first HCM disease gene, genetic studies still confirm that HCM is ...

    journal_title:Circulation research

    pub_type: 杂志文章,评审


    authors: Ashrafian H,McKenna WJ,Watkins H

    更新日期:2011-06-24 00:00:00

  • Heteromultimeric TRPC6-TRPC7 channels contribute to arginine vasopressin-induced cation current of A7r5 vascular smooth muscle cells.

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    journal_title:Circulation research

    pub_type: 杂志文章


    authors: Maruyama Y,Nakanishi Y,Walsh EJ,Wilson DP,Welsh DG,Cole WC

    更新日期:2006-06-23 00:00:00

  • The interferon stimulated gene 12 inactivates vasculoprotective functions of NR4A nuclear receptors.

    abstract:RATIONALE:Innate and adaptive immune responses alter numerous homeostatic processes that are controlled by nuclear hormone receptors. NR4A1 is a nuclear receptor that is induced in vascular pathologies, where it mediates protection. OBJECTIVE:The underlying mechanisms that regulate the activity of NR4A1 during vascula...

    journal_title:Circulation research

    pub_type: 杂志文章


    authors: Papac-Milicevic N,Breuss JM,Zaujec J,Ryban L,Plyushch T,Wagner GA,Fenzl S,Dremsek P,Cabaravdic M,Steiner M,Glass CK,Binder CJ,Uhrin P,Binder BR

    更新日期:2012-04-13 00:00:00

  • Nonuniformity of endothelial constitutive nitric oxide synthase distribution in cardiac endothelium.

    abstract::Endocardial endothelium and endothelium of coronary vessels produce NO. Histochemical methods have suggested that coronary arterial endothelial cells contain more endothelial constitutive NO synthase (ecNOS) than does coronary venous endothelium. We have further investigated the distribution of ecNOS in cardiac endoth...

    journal_title:Circulation research

    pub_type: 杂志文章


    authors: Andries LJ,Brutsaert DL,Sys SU

    更新日期:1998-02-09 00:00:00

  • Interaction of acidosis and increased extracellular potassium on action potential characteristics and conduction in guinea pig ventricular muscle.

    abstract::We studied the individual and combined effects of extracellular acidosis and increases in extracellular potassium on action potential characteristics and conduction in order to gain a better understanding of the effects of acute ischemia. At each level of potassium between 2.7 and 17 mm, acidosis induced by increasing...

    journal_title:Circulation research

    pub_type: 杂志文章


    authors: Kagiyama Y,Hill JL,Gettes LS

    更新日期:1982-11-01 00:00:00

  • Mechanical stimulation regulates voltage-gated potassium currents in cardiac microvascular endothelial cells.

    abstract::Vascular endothelial cells are constantly exposed to mechanical forces resulting from blood flow and transmural pressure. The goal of this study was to determine whether mechanical stimulation alters the properties of endothelial voltage-gated K+ channels. Cardiac microvascular endothelial cells (CMECs) were isolated ...

    journal_title:Circulation research

    pub_type: 杂志文章


    authors: Fan J,Walsh KB

    更新日期:1999-03-05 00:00:00

  • Angiotensin-converting enzyme activity and its modulation by oxygen tension in the guinea pig fetal-placental unit.

    abstract::Angiotensin-converting enzyme (ACE) activity in the guinea pig fetal-placental unit was assessed at the different oxygen tensions found in utero, during labor, and at birth. To determine fetal-placental ACE activity, we separately perfused in situ term guinea pig fetuses and their placentas via the umbilical vessels u...

    journal_title:Circulation research

    pub_type: 杂志文章,收录出版


    authors: Davidson D,Stalcup SA,Mellins RB

    更新日期:1981-02-01 00:00:00

  • Species-specific differences in positive and negative regulatory elements in the renin gene enhancer.

    abstract::A distal transcriptional enhancer has been previously reported upstream of the mouse renin gene. A homologous sequence is also present upstream of the human renin gene, but the mouse and human renin enhancers differ markedly in their ability to activate transcription of a renin promoter. Although the 2 enhancers share...

    journal_title:Circulation research

    pub_type: 杂志文章


    authors: Shi Q,Black TA,Gross KW,Sigmund CD

    更新日期:1999-09-17 00:00:00

  • An angiotensin II type 1 receptor mutant lacking epidermal growth factor receptor transactivation does not induce angiotensin II-mediated cardiac hypertrophy.

    abstract::We have shown previously that tyrosine 319 in a conserved YIPP motif in the C terminus of angiotensin II (Ang II) type 1 receptors (AT(1)Rs) is essential for transactivation of epidermal growth factor receptor (EGFR) in vitro. We hypothesized that the signaling mechanism mediated through the specific amino acid sequen...

    journal_title:Circulation research

    pub_type: 杂志文章


    authors: Zhai P,Galeotti J,Liu J,Holle E,Yu X,Wagner T,Sadoshima J

    更新日期:2006-09-01 00:00:00

  • Failure of atriopeptin II to cause arterial vasodilation in the conscious rat.

    abstract::The cardiovascular actions of the synthetic natriuretic peptide, atriopeptin II, were examined in conscious unrestrained spontaneously hypertensive rats and normotensive Wistar-Kyoto rats. The animals were chronically instrumented with miniaturized pulsed Doppler flow probes to allow measurement of regional blood flow...

    journal_title:Circulation research

    pub_type: 杂志文章


    authors: Lappe RW,Smits JF,Todt JA,Debets JJ,Wendt RL

    更新日期:1985-04-01 00:00:00

  • Activation of MAP kinase in vivo follows balloon overstretch injury of porcine coronary and carotid arteries.

    abstract::Vascular restenosis involves contraction, proliferation, and remodeling of the arterial wall in response to overstretch injury. Mitogen-activated protein kinases (MAPKs) are implicated in both contraction and proliferation of vascular smooth muscle (VSM), and studies of porcine carotid arterial muscle strips have show...

    journal_title:Circulation research

    pub_type: 杂志文章


    authors: Pyles JM,March KL,Franklin M,Mehdi K,Wilensky RL,Adam LP

    更新日期:1997-12-01 00:00:00

  • Regulation of the growth arrest and DNA damage-inducible gene 45 (GADD45) by peroxisome proliferator-activated receptor gamma in vascular smooth muscle cells.

    abstract::Peroxisome proliferator-activated receptor (PPAR) gamma is activated by thiazolidinediones (TZDs), widely used as insulin-sensitizing agents for the treatment of type 2 diabetes. TZDs have been shown to induce apoptosis in a variety of mammalian cells. In vascular smooth muscle cells (VSMCs), proliferation and apoptos...

    journal_title:Circulation research

    pub_type: 杂志文章


    authors: Bruemmer D,Yin F,Liu J,Berger JP,Sakai T,Blaschke F,Fleck E,Van Herle AJ,Forman BM,Law RE

    更新日期:2003-08-22 00:00:00

  • Reduced unloaded sarcomere shortening velocity and a shift to a slower myosin isoform in acute murine coxsackievirus myocarditis.

    abstract::We developed a mouse myocardial preparation to study cellular dysfunction in acute coxsackievirus myocarditis. Thin right ventricular papillary muscles from normal mice (n = 8) were compared with muscles from mice 7 days after coxsackievirus infection (n = 7). Sarcomere shortening was studied with laser diffraction (H...

    journal_title:Circulation research

    pub_type: 杂志文章


    authors: Hamrell BB,Huber SA,Leslie KO

    更新日期:1994-09-01 00:00:00

  • Small guanine nucleotide-binding proteins and myocardial hypertrophy.

    abstract::The small (21 kDa) guanine nucleotide-binding protein (small G protein) superfamily comprises 5 subfamilies (Ras, Rho, ADP ribosylation factors [ARFs], Rab, and Ran) that act as molecular switches to regulate numerous cellular responses. Cardiac myocyte hypertrophy is associated with cell growth and changes in the cyt...

    journal_title:Circulation research

    pub_type: 杂志文章,评审


    authors: Clerk A,Sugden PH

    更新日期:2000-05-26 00:00:00

  • Vascular endothelial growth factor-B promotes in vivo angiogenesis.

    abstract::Vascular endothelial growth factors (VEGFs) and their receptors have emerged as central regulators of the angiogenic process. However, involvement of VEGF-B, one of these factors, in angiogenesis remains obscure. Mice received subcutaneous injection of Matrigel alone or Matrigel with human recombinant protein rhVEGF-B...

    journal_title:Circulation research

    pub_type: 杂志文章


    authors: Silvestre JS,Tamarat R,Ebrahimian TG,Le-Roux A,Clergue M,Emmanuel F,Duriez M,Schwartz B,Branellec D,Lévy BI

    更新日期:2003-07-25 00:00:00

  • Chronic Kidney Disease Induces Inflammatory CD40+ Monocyte Differentiation via Homocysteine Elevation and DNA Hypomethylation.

    abstract:RATIONALE:Patients with chronic kidney disease (CKD) develop hyperhomocysteinemia and have a higher cardiovascular mortality than those without hyperhomocysteinemia by 10-fold. OBJECTIVE:We investigated monocyte differentiation in human CKD and cardiovascular disease (CVD). METHODS AND RESULTS:We identified CD40 as a...

    journal_title:Circulation research

    pub_type: 杂志文章


    authors: Yang J,Fang P,Yu D,Zhang L,Zhang D,Jiang X,Yang WY,Bottiglieri T,Kunapuli SP,Yu J,Choi ET,Ji Y,Yang X,Wang H

    更新日期:2016-11-11 00:00:00

  • Serum Beta Carotene and Overall and Cause-Specific Mortality.

    abstract:RATIONALE:Although the health effects of beta carotene have been studied extensively, a systematic examination of serum concentrations and long-term mortality, including cardiovascular disease mortality, has not been reported. OBJECTIVE:Explore whether serum beta carotene is associated with overall and cause-specific ...

    journal_title:Circulation research

    pub_type: 杂志文章


    authors: Huang J,Weinstein SJ,Yu K,Männistö S,Albanes D

    更新日期:2018-12-07 00:00:00

  • Characterization of [3H](+/-)carazolol binding to beta-adrenergic receptors. Application to study of beta-adrenergic receptor subtypes in canine ventricular myocardium and lung.

    abstract::[3H](+/-)Carazolol, a newly available beta-adrenergic receptor antagonist, can be used to characterize beta-adrenergic receptor subtypes present in membrane vesicles derived from canine ventricular myocardium and canine lung. [3H](+/-)Carazolol binding is saturable, of high affinity, and is displaceable by beta-adrene...

    journal_title:Circulation research

    pub_type: 杂志文章


    authors: Manalan AS,Besch HR Jr,Watanabe AM

    更新日期:1981-08-01 00:00:00

  • Anchoring proteins as regulators of signaling pathways.

    abstract::Spatial and temporal organization of signal transduction is coordinated through the segregation of signaling enzymes in selected cellular compartments. This highly evolved regulatory mechanism ensures the activation of selected enzymes only in the vicinity of their target proteins. In this context, cAMP-responsive tri...

    journal_title:Circulation research

    pub_type: 杂志文章,评审


    authors: Perino A,Ghigo A,Scott JD,Hirsch E

    更新日期:2012-08-03 00:00:00

  • Contribution of Na(+)-Ca2+ exchange to stimulation of transient inward current by isoproterenol in rabbit cardiac Purkinje fibers.

    abstract::Cellular mechanisms underlying beta-adrenergic stimulation of the arrhythmogenic transient inward current (TI) were investigated by using a two-microelectrode voltage-clamp technique in rabbit cardiac Purkinje fibers. TI induced by elevating [Ca2+]o to 30 mmol/L and substituting [Na+]o with N-methyl-D-glucamine (NMG) ...

    journal_title:Circulation research

    pub_type: 杂志文章


    authors: Han X,Ferrier GR

    更新日期:1995-04-01 00:00:00

  • MicroRNA regulation of cardiovascular development.

    abstract::The transcriptional regulation of cardiovascular development requires precise spatiotemporal control of gene expression, and heterozygous mutations of transcription factors have frequently been implicated in human cardiovascular malformations. A novel mechanism involving posttranscriptional regulation by small, noncod...

    journal_title:Circulation research

    pub_type: 杂志文章,评审


    authors: Cordes KR,Srivastava D

    更新日期:2009-03-27 00:00:00