Abstract:
:A gene encoding mature human insulin-like growth factor II (IGF-II) was constructed from the modified IGF-II cDNA sequence and two double-stranded synthetic oligodeoxynucleotide linkers. It was fused to a truncated lacZ gene such that IGF-II was expressed as part of C-terminus of beta-galactosidase. This fused lacZ'-IGF-II gene was under the control of tac promoter and we overproduced the beta-galactosidase-IGF-II fusion protein in the Escherichia coli. The fusion protein formed inclusion bodies inside the cells. The fusion protein was purified from the isolated inclusion bodies and IGF-II protein was obtained from their fusion protein by CNBr cleavage. The released IGF-II was confirmed by its molecular weight as determined by SDS-PAGE and by its ability to bind anti-IGF antibody.
journal_name
J Biotechnoljournal_title
Journal of biotechnologyauthors
Rhee HJ,Lee YI,Yang KHdoi
10.1016/0168-1656(90)90077-osubject
Has Abstractpub_date
1990-03-01 00:00:00pages
293-304issue
4eissn
0168-1656issn
1873-4863pii
0168-1656(90)90077-Ojournal_volume
13pub_type
杂志文章abstract::The Pichia anomala gene PPHY, which codes for a cell-bound phytase, was isolated from genomic DNA by PCR, using oligonucleotide sequences derived from the N-terminal region of the purified phytase protein (Pphyp) and a degenerate primer derived from conserved sequences of yeast and fungal phytases as primers. The gene...
journal_title:Journal of biotechnology
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journal_title:Journal of biotechnology
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journal_title:Journal of biotechnology
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journal_title:Journal of biotechnology
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更新日期:2014-12-20 00:00:00
abstract::A gene encoding for hirudin, a potent thrombin inhibitor, was expressed in Escherichia coli, which is the most widely used host. When the recombinant hirudin analog, CX-397, was overproduced by E. coli (600 mg l(-1)) in the absence of nutrient amino acids in the culture medium, the presence of two derivatives in the f...
journal_title:Journal of biotechnology
pub_type: 杂志文章
doi:10.1016/s0168-1656(01)00396-0
更新日期:2002-02-14 00:00:00
abstract::Actinoplanes sp. SE50/110 is the natural producer of acarbose, which is used in the treatment of diabetes mellitus type II. However, until now the transcriptional organization and regulation of the acarbose biosynthesis are only understood rudimentarily. The genome sequence of Actinoplanes sp. SE50/110 was known befor...
journal_title:Journal of biotechnology
pub_type: 杂志文章
doi:10.1016/j.jbiotec.2017.04.013
更新日期:2017-06-10 00:00:00
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journal_title:Journal of biotechnology
pub_type: 杂志文章
doi:10.1016/j.jbiotec.2014.09.020
更新日期:2014-12-20 00:00:00
abstract::Deracemization of D,L-phosphinothricin (D,L-PPT) is one of the most promising routes for preparation of optically pure L-PPT. In this work, an efficient multi-enzyme redox cascade was developed for deracemization ofPPT, which includes oxidative reaction and reductive reaction. The oxidative reaction catalyzing oxidati...
journal_title:Journal of biotechnology
pub_type: 杂志文章
doi:10.1016/j.jbiotec.2020.09.024
更新日期:2021-01-10 00:00:00
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journal_title:Journal of biotechnology
pub_type: 杂志文章
doi:10.1016/j.jbiotec.2006.04.021
更新日期:2006-11-01 00:00:00
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journal_title:Journal of biotechnology
pub_type: 杂志文章
doi:10.1016/j.jbiotec.2008.08.001
更新日期:2008-11-25 00:00:00
abstract::The combination of anaerobic and aerobic periods in the operation cycle of a Sequencing Batch Reactor (SBR) was chosen to study biological color removal from simulated textile effluents containing reactive, sulfonated, monoazo and diazo dyes, respectively, Remazol Brilliant Violet 5R and Remazol Black B. 90% color rem...
journal_title:Journal of biotechnology
pub_type: 杂志文章
doi:10.1016/s0168-1656(01)00313-3
更新日期:2001-08-23 00:00:00
abstract::Because of their ecological functions, natural products have been optimized in evolution for interaction with biological systems and receptors. However, they have not necessarily been optimized for other desirable drug properties and thus can often be improved by structural modification. Using examples from the litera...
journal_title:Journal of biotechnology
pub_type: 杂志文章,评审
doi:10.1016/j.jbiotec.2005.12.001
更新日期:2006-06-25 00:00:00
abstract::Roseateles depolymerans is a Gram-negative, rod-shaped, flagellated, obligately aerobic, photosynthetic bacterium that was isolated from the Hanamuro River, Ibaraki Prefecture, Japan. Here, we report the complete genome of R. depolymerans KCTC 42856(T) (=61A(T)=DSM 11813(T)=CCUG 48747(T)=NCIMB 13588(T)), which consist...
journal_title:Journal of biotechnology
pub_type: 杂志文章
doi:10.1016/j.jbiotec.2016.01.012
更新日期:2016-02-20 00:00:00
abstract::MutS as a mismatch binding protein is a promising tool for SNP detection. Green fluorescent protein (GFP) is known as an excellent reporter domain. We constructed chimeric proteins consisting of MutS from Thermus thermophilus and GFPuv from Aequorea victoria by cloning the GFPuv gene into the plasmid vectors carrying ...
journal_title:Journal of biotechnology
pub_type: 杂志文章
doi:10.1016/j.jbiotec.2005.08.009
更新日期:2006-01-24 00:00:00
abstract::Enzymatic hydrolysis constitutes an attractive strategy for biorefining of abundant, low-cost agricultural by-products such as wheat bran and straw. However, to adopt such an approach, efficient enzymes are required, in particular xylanases. To promote heat-induced disorganization of the complex cell wall network in w...
journal_title:Journal of biotechnology
pub_type: 杂志文章
doi:10.1016/j.jbiotec.2006.03.025
更新日期:2006-09-18 00:00:00
abstract::Epigallocatechin-3-O-gallate (EGCg) and related polyphenolic compounds found in tea are known to have antioxidative activities. However, they also have pro-oxidative activities such as generation of hydrogen peroxide. In this report, we investigated the effect on cells and showed the potential usage of EGCg in cell pr...
journal_title:Journal of biotechnology
pub_type: 杂志文章
doi:10.1016/j.jbiotec.2006.08.006
更新日期:2007-01-20 00:00:00
abstract::A new β-glucosidase gene (bglSp) was cloned from the ginsenoside converting Sphingomonas sp. strain 2F2 isolated from the ginseng cultivating filed. The bglSp consisted of 1344 bp (447 amino acid residues) with a predicted molecular mass of 49,399 Da. A BLAST search using the bglSp sequence revealed significant homolo...
journal_title:Journal of biotechnology
pub_type: 杂志文章
doi:10.1016/j.jbiotec.2011.07.024
更新日期:2011-11-10 00:00:00
abstract::A computer system solution for integration of a distributed bioreactor monitoring and control instrumentation on the laboratory scale is described. Bioreactors equipped with on-line analyzers for mass spectrometry, near-infrared spectroscopy, electrochemical probes and multi-array gas sensors and their respective soft...
journal_title:Journal of biotechnology
pub_type: 杂志文章
doi:10.1016/s0168-1656(03)00121-4
更新日期:2003-08-15 00:00:00
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journal_title:Journal of biotechnology
pub_type: 杂志文章
doi:10.1016/0168-1656(92)90007-v
更新日期:1992-11-01 00:00:00
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journal_title:Journal of biotechnology
pub_type: 杂志文章
doi:10.1016/j.jbiotec.2005.03.007
更新日期:2005-07-21 00:00:00
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journal_title:Journal of biotechnology
pub_type: 杂志文章
doi:10.1016/j.jbiotec.2020.01.004
更新日期:2020-02-10 00:00:00
abstract::The development and optimization of a purification process of monoclonal antibodies based on two continuous chromatography steps for capture and intermediate purification are presented. The two chromatography steps were individually optimized using either batch chromatography or sequential multicolumn chromatography (...
journal_title:Journal of biotechnology
pub_type: 杂志文章
doi:10.1016/j.jbiotec.2015.04.026
更新日期:2015-11-10 00:00:00
abstract::The use of the fungus Aspergillus niger for the bioleaching of heavy metals from spent catalyst was investigated, with fluid catalytic cracking (FCC) catalyst as a model. Bioleaching was examined in batch cultures with the spent catalysts at various pulp densities (1-12%). Chemical leaching was also performed using mi...
journal_title:Journal of biotechnology
pub_type: 杂志文章
doi:10.1016/j.jbiotec.2004.10.008
更新日期:2005-03-16 00:00:00
abstract::A highly active and stable cross-linked enzyme aggregates (CLEAs) of epoxide hydrolases (EHs) from Mung bean, which plays a crucial role in synthesis of valuable enantiopure diols, were successfully prepared and characterized. Under the optimum preparation conditions, the activity recovery of CLEAs recorded 92%. The C...
journal_title:Journal of biotechnology
pub_type: 杂志文章
doi:10.1016/j.jbiotec.2013.04.015
更新日期:2013-06-20 00:00:00
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journal_title:Journal of biotechnology
pub_type: 杂志文章,评审
doi:10.1016/0168-1656(92)90114-o
更新日期:1992-08-01 00:00:00
abstract::To study the metaproteome of a biogas-producing microbial community, fermentation samples were taken from an agricultural biogas plant for microbial cell and protein extraction and corresponding metagenome analyses. Based on metagenome sequence data, taxonomic community profiling was performed to elucidate the composi...
journal_title:Journal of biotechnology
pub_type: 杂志文章
doi:10.1016/j.jbiotec.2016.06.014
更新日期:2016-08-10 00:00:00
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journal_title:Journal of biotechnology
pub_type: 杂志文章
doi:10.1016/j.jbiotec.2005.07.023
更新日期:2006-02-10 00:00:00
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journal_title:Journal of biotechnology
pub_type: 杂志文章
doi:10.1016/j.jbiotec.2016.01.026
更新日期:2016-03-10 00:00:00
abstract::The use of a proper signal sequence is one of the major determinants for the efficient secretion of heterologous proteins from yeast. The signal sequence derived from inulinase (INU1A) of Kluyveromyces marxianus was evaluated in directing the secretion of a human glycoprotein, alpha 1-antitrypsin (alpha 1-AT), from Sa...
journal_title:Journal of biotechnology
pub_type: 杂志文章
doi:10.1016/0168-1656(96)01391-0
更新日期:1996-07-18 00:00:00
abstract::Skeletal muscle is becoming an attractive target tissue for gene therapy. Nevertheless, the low level of gene therapeutic expression in this tissue is the major limitation to it becoming an ideal target for gene transfer. The promoter is important element for gene transcription; however, the gene expression efficienci...
journal_title:Journal of biotechnology
pub_type: 杂志文章
doi:10.1016/j.jbiotec.2011.11.019
更新日期:2012-04-15 00:00:00