Abstract:
:Sarcoidosis is a multisystem disease of unknown etiology characterized by non-caseating granulomata, formed mainly from macrophages surrounded by lymphocytes and plasma cells. Using a novel method for the preparation of blood leucocytes for flow cytometry, we report increased expression of LeuCAMs (CD11/CD18) on peripheral blood leucocytes of 11 Caucasian and 10 Afro-Caribbean patients with sarcoidosis compared with age-, sex- and race-matched controls. Whilst the percentages of the cells expressing CD11/CD18 were no different, the density, expressed as mean fluorescence intensity (MFI), was greater for all leucocytes in sarcoids than in normal individuals. The expression of intercellular adhesion molecule-1 (ICAM-1), a ligand for LFA-1 which is expressed on all leucocytes, was not significantly different from normal, whereas HLA-DR was expressed more intensely on sarcoid monocytes (P less than 0.01) and blood lymphocytes (P less than 0.005) than control cells. Our findings are consistent with leucocyte activation although we were unable to confirm reports of elevated tumour necrosis factor-alpha (TNF-alpha) in the patients' plasma using an ELISA. Increased expression of adhesion molecules on peripheral blood leucocytes may play a role in the cellular extravasation, aggregation, and granuloma formation seen in sarcoidosis.
journal_name
Clin Exp Immunoljournal_title
Clinical and experimental immunologyauthors
Shakoor Z,Hamblin ASdoi
10.1111/j.1365-2249.1992.tb05839.xsubject
Has Abstractpub_date
1992-10-01 00:00:00pages
99-105issue
1eissn
0009-9104issn
1365-2249journal_volume
90pub_type
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更新日期:1986-02-01 00:00:00
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更新日期:1985-09-01 00:00:00
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journal_title:Clinical and experimental immunology
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更新日期:1983-01-01 00:00:00
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journal_title:Clinical and experimental immunology
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更新日期:1983-04-01 00:00:00
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更新日期:1980-11-01 00:00:00