Abstract:
:The IL-15 triggering effect of gliadin is not exclusive to coeliac disease (CD) patients, whereas the secondary response is CD specific. We have studied the expression of the IL-15 receptor, and the IL-15 response upon stimulation, in non-CD and CD patients, and the possible existence of a lower immunological threshold in the latter. Forty-two CD patients (20 on a gluten-containing diet, GCD, and 22 on gluten-free diet, GFD) and 24 non-CD healthy individuals were studied. IL15R alpha mRNA expression, and tissue characterization, were assayed in the duodenum. Biopsies from six CD patients on GFD and 10 non-CD individuals were studied in vitro using organ culture in basal conditions, as well as after IL-15 stimulation discarding basal IL-15 production. Secretion of immune mediators was measured in the culture supernatants. IL15R alpha mRNA expression was increased in CD patients, as compared with non-CD controls (on GFD P = 0.0334, on GCD P = 0.0062, respectively), and confirmed also by immunofluorescence. No differences were found between CD patients on GFD and on GCD. After in vitro IL-15 stimulation, IL15R alpha expression was only triggered in non-CD controls (P = 0.0313), though it remained increased in CD patients. Moreover, IL-15 induced a more intense immunological response in CD patients after triggering the production of both nitrites and IFN gamma (P = 0.0313, P = 0.0313, respectively). Gliadin-induced IL15 has a lower response threshold in CD patients, leading to the production of other immune mediators and the development of the intestinal lesion, and thus magnifying its effects within the CD intestine.
journal_name
Clin Exp Immunoljournal_title
Clinical and experimental immunologyauthors
Bernardo D,Garrote JA,Allegretti Y,León A,Gómez E,Bermejo-Martin JF,Calvo C,Riestra S,Fernández-Salazar L,Blanco-Quirós A,Chirdo F,Arranz Edoi
10.1111/j.1365-2249.2008.03743.xsubject
Has Abstractpub_date
2008-10-01 00:00:00pages
64-73issue
1eissn
0009-9104issn
1365-2249pii
CEI3743journal_volume
154pub_type
杂志文章abstract::Cell death and the release of chromatin have been demonstrated to activate the immune system producing autoantibodies against nuclear antigens in patients with systemic lupus erythematosus (SLE). Apoptosis, necrosis, necroptosis, secondary necrosis, autophagy and the clearance of dying cells by phagocytosis are proces...
journal_title:Clinical and experimental immunology
pub_type: 杂志文章,评审
doi:10.1111/cei.12417
更新日期:2015-01-01 00:00:00
abstract::Thirty-four out of fifty-two vasectomized men studied previously were studied again about 5 years after vasectomy for sperm-agglutinating and sperm-immoblizing antibodies, and for antibodies to human protamine. Only one man lost his already weak antibody activity, whilst one out of eight men, negative at 1 year, appea...
journal_title:Clinical and experimental immunology
pub_type: 杂志文章
doi:
更新日期:1979-10-01 00:00:00
abstract::Stress known to stimulate sympathetic activity, as well as the hypothalamic-pituitary-adrenal axis (HPA), produces a significant increase in adrenomedullin (AdM) levels, suggesting a regulatory or protective role for AdM in countering HPA activation that follows a variety of stressors. Stressors can modulate the secre...
journal_title:Clinical and experimental immunology
pub_type: 杂志文章
doi:10.1111/j.1365-2249.2010.04156.x
更新日期:2010-07-01 00:00:00
abstract::Autoantibodies to insulin are often the first autoantibodies detected in young children with type 1 diabetes and can be present before the onset of clinical diabetes. These autoantibodies and their epitopes are, however, not well characterized. We explored the use of monoclonal antibodies and their recombinant Fab as ...
journal_title:Clinical and experimental immunology
pub_type: 杂志文章
doi:10.1111/j.1365-2249.2005.02802.x
更新日期:2005-06-01 00:00:00
abstract::In this study the antigen-presenting capacity of human peritoneal cells and the influence of continuous ambulant peritoneal dialysis (CAPD) were studied. On average 6% of the peritoneal cells were dendritic cells (DC), with no difference between CAPD and control peritoneal cells. DC were enriched by selecting for non-...
journal_title:Clinical and experimental immunology
pub_type: 杂志文章
doi:10.1111/j.1365-2249.1993.tb03460.x
更新日期:1993-11-01 00:00:00
abstract::Myelin-reactive T cells are considered to play an essential role in the pathogenesis of multiple sclerosis (MS), an autoimmune disease of the central nervous system. We have previously studied the effects of T cell vaccination (TCV), a procedure by which MS patients are immunized with attenuated autologous myelin basi...
journal_title:Clinical and experimental immunology
pub_type: 杂志文章
doi:10.1046/j.1365-2249.2003.02019.x
更新日期:2003-01-01 00:00:00
abstract::Myotonic dystrophy (MyD), an autosomal dominant neuromuscular disease with multisystem abnormalities, is associated with hypercatabolism of IgG. The hypercatabolism is not related to structural abnormalities of the IgG molecule in MyD but appears to be due to a derangement of the serum IgG concentration-fractional cat...
journal_title:Clinical and experimental immunology
pub_type: 杂志文章
doi:
更新日期:1982-12-01 00:00:00
abstract::A simple and efficient method of extracting complement-fixing immune complexes (IC) from whole serum and recovering them has been developed. 125I-labelled, in vitro prepared IC in whole serum were incubated with Sepharose 4B covalently linked to monoclonal anti-C3g or anti-C1q and the binding and recovery of IC was mo...
journal_title:Clinical and experimental immunology
pub_type: 杂志文章
doi:
更新日期:1986-08-01 00:00:00
abstract::In recent years galectin-3 has gained attention as a signalling molecule, mainly in inflammatory diseases. Data on galectin-3 expression in neonates, however, are limited, and expression of this lectin in cord blood has not yet been reported. The aim of this study was to determine galectin-3 levels in cord blood of te...
journal_title:Clinical and experimental immunology
pub_type: 杂志文章
doi:10.1111/j.1365-2249.2011.04509.x
更新日期:2012-02-01 00:00:00
abstract::Fractalkine (FKN/CX3CL1) has been detected in synovial fluids from osteoarthritis (OA) patients. Additionally, low-level expression of the FKN receptor, CX3CR1, has been demonstrated in OA synovial lining. This study aimed to determine a biological function for this ligand/receptor pair in OA and to assess a potential...
journal_title:Clinical and experimental immunology
pub_type: 杂志文章
doi:10.1111/j.1365-2249.2009.03903.x
更新日期:2009-05-01 00:00:00
abstract::There are estimated to be approximately 1500 people in the United Kingdom with C1 inhibitor (C1INH) deficiency. At BartsHealth National Health Service (NHS) Trust we manage 133 patients with this condition and we believe that this represents one of the largest cohorts in the United Kingdom. C1INH deficiency may be her...
journal_title:Clinical and experimental immunology
pub_type: 杂志文章
doi:10.1111/cei.12083
更新日期:2013-07-01 00:00:00
abstract::Reliable risk assessment for biotherapeutics requires accurate evaluation of risk factors associated with immunogenicity. Immunogenicity risk assessment tools were developed and applied to investigate the immunogenicity of a fully human therapeutic monoclonal antibody, ATR-107 [anti-interleukin (IL)-21 receptor] that ...
journal_title:Clinical and experimental immunology
pub_type: 杂志文章
doi:10.1111/cei.12711
更新日期:2016-01-01 00:00:00
abstract::Thyroid peroxidase purified from porcine thyroid (pTPO) was found to induce an experimental murine thyroiditis with genetic restriction which was very different from that induced by mouse thyroglobulin (mTg). C57BL/6 and C57BL/10 (both H-2b) were good responders for thyroiditis, whereas A/J (H-2a), BALB/c (H-2d), DBA/...
journal_title:Clinical and experimental immunology
pub_type: 杂志文章
doi:10.1111/j.1365-2249.1990.tb06434.x
更新日期:1990-04-01 00:00:00
abstract::Previous studies show that endoplasmic reticulum-associated aminopeptidase (ERAP1/ERAP2) and runt-related transcription factor 3 (RUNX3) gene polymorphisms are associated with AS (ankylosing spondylitis) in European Caucasians. However, contradictory results were reported in different Asian populations. The purpose of...
journal_title:Clinical and experimental immunology
pub_type: 杂志文章
doi:10.1111/cei.13121
更新日期:2018-07-01 00:00:00
abstract::Anti-tubulin antibodies were studied in normal human serum either maintained at neutral pH to measure the free antibody activity (FAA) or treated at pH 2.8 to measure the total antibody activity (TAA): FAA + the antibody activity in the form of immune complexes (ICAA). Anti-tubulin antibody activities were assessed by...
journal_title:Clinical and experimental immunology
pub_type: 杂志文章
doi:
更新日期:1988-02-01 00:00:00
abstract::T and B cell function, in particular IgD production in vitro, were studied across the spectrum of HIV infection in homosexual men and compared with seronegative homosexual and heterosexual male controls. Proliferation to phytohaemagglutinin (PHA) was reduced most strikingly in symptomatic HIV infection; it was also im...
journal_title:Clinical and experimental immunology
pub_type: 杂志文章
doi:
更新日期:1989-01-01 00:00:00
abstract::An immunoglobulin phage display library constructed from a tumour-associated pericolic lymph node was panned against the extracellular domain of the oncoprotein c-erbB-2. Sixteen independent clones were confirmed as positive binders based on ELISA analysis of soluble Fabs. Nucleotide sequencing demonstrated that the V...
journal_title:Clinical and experimental immunology
pub_type: 杂志文章
doi:10.1046/j.1365-2249.1997.4161316.x
更新日期:1997-07-01 00:00:00
abstract::Vaccination against meningococcal serogroup B is recommended for patients with a complement deficiency; however, although immunogenicity in this patient group has been shown, efficacy has not yet been established. In this study, we collected serum from children with a complement deficiency in the alternative pathway o...
journal_title:Clinical and experimental immunology
pub_type: 杂志文章
doi:10.1111/cei.13368
更新日期:2019-12-01 00:00:00
abstract::An improved in vitro technique to assay for migration inhibitory factor is presented. The method employs chromium-51-radiolabelled guinea-pig macrophages and offers significant advantages including (1) elimination of observer to observer variation and tedious measurements resulting in an objective and technically simp...
journal_title:Clinical and experimental immunology
pub_type: 杂志文章
doi:
更新日期:1980-06-01 00:00:00
abstract::Cell mediated immunity (CMI) to hepatitis B viral antigens was studied in BALB/mice after immunization with purified hepatitis B surface antigen (HBsAg), or core antigen (HBcAg), with adjuvants. The two in vitro assays for cell-mediated immunity (CMI), utilizing lymph node cells, were release of interferon after expos...
journal_title:Clinical and experimental immunology
pub_type: 杂志文章
doi:
更新日期:1986-05-01 00:00:00
abstract::A series of polyclonal and monoclonal anti-human IgE sera reacted with a panel of 11 selected IgA and IgM producing B lymphoblastoid cell lines (BLCLs) in cytoplasmic fluorescence, ELISA and immunoprecipitation assays. The reactions were discordant between the BLCLs and the individual antisera. The panel of 11 BLCLs w...
journal_title:Clinical and experimental immunology
pub_type: 杂志文章
doi:
更新日期:1988-11-01 00:00:00
abstract::Human immunodeficiency virus (HIV)-1 protease is a known target of CD8+ T cell responses, but it is the only HIV-1 protein in which no fully characterized HIV-1 protease CD4 epitopes have been identified to date. We investigated the recognition of HIV-1 protease by CD4+ T cells from 75 HIV-1-infected, protease inhibit...
journal_title:Clinical and experimental immunology
pub_type: 杂志文章
doi:10.1111/j.1365-2249.2011.04319.x
更新日期:2011-04-01 00:00:00
abstract::The humoral immune response, especially IgG and IgA, is considered to be protective in the pathogenesis of periodontal disease, but the precise mechanisms are still unknown. Immunoglobulins arriving at the periodontal lesion are from both systemic and local tissue sources. In order to understand better the local immun...
journal_title:Clinical and experimental immunology
pub_type: 杂志文章
doi:10.1046/j.1365-2249.1999.00819.x
更新日期:1999-03-01 00:00:00
abstract::The carcinoembryonic antigen (CEA) distribution obtained after fractionation of thirty sera of patients with gastrointestinal tumours by gel filtration in Sephadex G200 was compared with that resulting from extraction of the sera with perchloric acid. In all cases gel filtration yielded a fraction of free CEA and in t...
journal_title:Clinical and experimental immunology
pub_type: 杂志文章
doi:
更新日期:1979-07-01 00:00:00
abstract::Delayed hypersensitivity skin reactions to tuberculin when injected alone or in mixture with antigens of M. leprae were examined in leprosy patients and in healthy controls. The tuberculin reaction was significantly inhibited in more than one half of both LL and BT patients by the soluble extract of M. leprae (leprosi...
journal_title:Clinical and experimental immunology
pub_type: 杂志文章
doi:
更新日期:1987-04-01 00:00:00
abstract::Antibody reactivity against the 'mitochondrial M2 antigen' was determined in sera from 10 patients with primary biliary cirrhosis (PBC), using Western blotting after SDS-PAGE separation of rat liver mitochondria (RLM) and plasma membrane proteins. The molecular weights of the major M2 antigens in rat liver mitochondri...
journal_title:Clinical and experimental immunology
pub_type: 杂志文章
doi:10.1111/j.1365-2249.1991.tb05652.x
更新日期:1991-03-01 00:00:00
abstract::Antibody responses in immunoglobulin G1, G2, G3, G4, A (IgA1) and M isotypes were studied in 10 patients with an acute influenza A and in another 10 patients with a parainfluenza type 1 virus infection using radioimmunoassay with standardized monoclonal anti-immunoglobulins. A four-fold or greater increase of antibody...
journal_title:Clinical and experimental immunology
pub_type: 杂志文章
doi:
更新日期:1985-04-01 00:00:00
abstract::A preparation of the putative antibody in HN (Heymann's nephritis) lacking preformed immune complexes, obtained from the acid eluates of kidneys with HN, purified through ion exchange and gel-filtration chromatography, characterized by immunoelectrophoresis, sucrose density-gradient ultracentrifugation, 125I-Clq assay...
journal_title:Clinical and experimental immunology
pub_type: 杂志文章
doi:
更新日期:1982-02-01 00:00:00
abstract::Idiopathic histaminergic acquired angioedema (IH-AAE) is a common cause of recurrent angioedema without wheals. It is a mast cell-mediated disease thought to belong to the same clinical entity as chronic urticaria (CU). The objective of this study was to describe the clinical and epidemiological characteristics of IH-...
journal_title:Clinical and experimental immunology
pub_type: 杂志文章
doi:10.1111/cei.12789
更新日期:2016-07-01 00:00:00
abstract::Commercial cellular tests are used to diagnose Lyme borreliosis (LB), but studies on their clinical validation are lacking. This study evaluated the utility of an in-house and a commercial enzyme-linked immunosorbent spot (ELISpot) assay for the diagnosis of Lyme neuroborreliosis (LNB). Prospectively, peripheral blood...
journal_title:Clinical and experimental immunology
pub_type: 杂志文章
doi:10.1111/cei.13393
更新日期:2020-03-01 00:00:00