Predictive value of human papillomavirus DNA detection by filter hybridization and polymerase chain reaction in women with negative results of colposcopic examination.

Abstract:

:This study focused on women who had an abnormal Papanicolaou smear and a subsequent colposcopic examination negative for a cervical squamous intraepithelial lesion. Cervical swabs obtained during the initial colposcopic examination were analyzed for human papillomavirus (HPV) DNA, and these results were correlated with the follow-up examinations. Human papillomavirus DNA was detected by dot-blot hybridization in 31 of 109 (28%) cases. The rate of subsequent biopsy-proved squamous intraepithelial lesion was 17 of 31 (55%) for the HPV-positive group compared with 9 of 78 (11%) for the HPV-negative group. The HPV-DNA detection rate by polymerase chain reaction was 46 of 109 (42%), with a rate of subsequent squamous intraepithelial lesion of 18 of 46 (39%) for the HPV-positive group, compared with 8 of 63 (13%) for the HPV-negative group. Most women had no evidence of squamous intraepithelial lesions on follow-up if the concurrent Papanicolaou smear was normal, even when HPV DNA was detected, suggesting that HPV detection per se does not necessarily correlate with subsequent squamous intraepithelial lesion.

journal_name

Am J Clin Pathol

authors

Nuovo GJ,Moritz J,Walsh LL,MacConnell P,Koulos J

doi

10.1093/ajcp/98.5.489

subject

Has Abstract

pub_date

1992-11-01 00:00:00

pages

489-92

issue

5

eissn

0002-9173

issn

1943-7722

journal_volume

98

pub_type

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