Abstract:
:IGF-I is an endocrine and paracrine regulator of skeletal homeostasis, principally by virtue of its anabolic effects on osteoblastic cells. In the current study, we examined the intracellular signaling pathways by which IGF-I promotes proliferation and survival in SaOS-2 human osteoblastic cells. Inhibition of each of the phosphatidylinositol-3 kinase (PI-3 kinase), p42/44 MAPK, and p70s6 kinase pathways partially inhibited the ability of IGF-I to stimulate osteoblast proliferation and survival. Because activation of p70s6 kinase is downstream of both PI-3 kinase and p42/44 MAPK activation in osteoblasts treated with IGF-I, this ribosomal kinase represents a convergence point for IGF-I-induced PI-3 kinase and p42/44 MAPK signaling in osteoblastic cells. In addition, abrogation of PI-3 kinase-dependent Akt signaling, which does not inhibit IGF-I-induced p70s6 kinase phosphorylation, also inhibited the antiapoptotic effects of IGF-I in osteoblasts. Finally, interruption of G beta gamma signaling partially abrogated the ability of IGF-I to promote osteoblast survival, without inhibiting signaling through PI-3 kinase/Akt, p42/44 MAPKs, or p70s6 kinase. These data suggest that IGF-I signals osteoblast mitogenesis and survival through parallel, partly overlapping intracellular pathways involving PI-3 kinase, p42/44 MAPKs, and G beta gamma subunits.
journal_name
Endocrinologyjournal_title
Endocrinologyauthors
Grey A,Chen Q,Xu X,Callon K,Cornish Jdoi
10.1210/en.2003-0350subject
Has Abstractpub_date
2003-11-01 00:00:00pages
4886-93issue
11eissn
0013-7227issn
1945-7170pii
en.2003-0350journal_volume
144pub_type
杂志文章相关文献
ENDOCRINOLOGY文献大全abstract::Bilateral destruction of the hypothalamic paraventricular nuclei (PVN) produced a profound depression of plasma TSH and the median eminence TRH concentration in hypothyroid rats. Anterior pituitary type II iodothyronine 5'-deiodinase (5'-D) activity was consistently lower but not significantly different in sham- and P...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-123-3-1676
更新日期:1988-09-01 00:00:00
abstract::The rapid, nongenomic effects of estrogen are increasingly recognized as playing an important role in several aspects of estrogen action. Rapid activation of the mitogen-activated protein kinase (MAPK) signaling pathway by estrogen is among the more recently identified of these effects. To explore the role of estrogen...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.142.6.8071
更新日期:2001-06-01 00:00:00
abstract::Recent studies have revealed that C-type natriuretic peptide (CNP) is a potent stimulator of endochondral bone growth. Nevertheless, the effect of CNP on bone turnover has not yet been well studied. To elucidate this issue, we investigated the bone phenotype of a mouse model with elevated plasma CNP concentrations (SA...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2014-1801
更新日期:2015-07-01 00:00:00
abstract::Adiposity is regulated in a sexually divergent manner. This is partly due to sex steroids, but the differential effects of androgens in males and females are unclear. We investigated effects of testosterone on energy balance in castrated male (n = 6) and female sheep (n = 4), which received 3 × 200 mg testosterone imp...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2011-1634
更新日期:2012-01-01 00:00:00
abstract::A variety of transcription factors including Wilms tumor gene (Wt-1), steroidogenic factor 1 (Sf-1), dosage-sensitive sex reversal, adrenal hypoplasia congenita on the X-chromosome, Gene 1 (Dax-1), and pre-B-cell transcription factor 1 (Pbx1) have been defined as necessary for regular adrenocortical development. Howev...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2006-0681
更新日期:2007-02-01 00:00:00
abstract::Proopiomelanocortin (POMC) neurons in the hypothalamus are direct targets of the adipostatic hormone leptin and contribute to energy homeostasis by integrating peripheral and central information. The melanocortin and beta-endorphin neuropeptides are processed from POMC and putatively coreleased at axon terminals. Mela...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2002-221096
更新日期:2003-05-01 00:00:00
abstract::Tumor progression largely depends on blood supply and neovessel formation, and angiogenesis is emerging as a promising target for cancer therapy. Vascular endothelial growth factor (VEGF), a major proangiogenic molecule, stimulates angiogenesis via promoting endothelial proliferation, survival and migration. VEGF has ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2003-1106
更新日期:2004-02-01 00:00:00
abstract::The antiestrogen ICI 164,384 (ICI) binds the estrogen receptor (ER) with approximately 20% the affinity of estradiol, but without the partial agonistic effects caused by tamoxifen. Investigations into the mechanism of ICI action have used ER molecules expressed in vitro to examine the binding of ER to ICI and the capa...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-129-4-2000
更新日期:1991-10-01 00:00:00
abstract::Experimental evidence indicates that the neonatal gonadal steroid environment is an important determinant of the sexual dimorphism of GH secretion and body growth. However, the influence of the sex steroids in GH control during adult life and their mechanism/site of action are largely unknown. In the present study we ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.130.1.1345780
更新日期:1992-01-01 00:00:00
abstract::A competitive protein-binding (CPB) assay, suitable for measuring corticosterone levels in 20 mul of mouse plasma or 100 mg of brain, is described. The postnatal development of adrenocortical function was determined in C57BL/10 and DBA/1 mice by CPB assay of basal and stressinduced levels of plasma corticosterone and ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-98-6-1434
更新日期:1976-06-01 00:00:00
abstract::We characterized four antipeptide polyclonal antibodies (abs) able to specifically recognize each thyroid hormone receptor (TR) isoform. The abs immunoprecipitated both the in vitro synthesized receptor and the receptor expressed in E. coli and their specificity was confirmed by competition studies and immunohistochem...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.131.5.1425440
更新日期:1992-11-01 00:00:00
abstract::The possibility that the responsiveness of plasma aldosterone concentration to angiotensin II alters with changes in sodium balance was investigated in male beagle dogs under conditions of controlled sodium and potassium intake. Angiotensin II was infused at four different rates (usually 3, 6, 12, and 24 ng/kg/min), e...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-102-2-485
更新日期:1978-02-01 00:00:00
abstract::At micromolar (pharmacological) concentrations, the action of tamoxifen on the proliferation of estrogen-dependent cells can be mediated not only by the estrogen receptor (ER), but also by other target molecules, such as protein kinase-C (PKC), which are easily inhibited by antiestrogens in cell-free experiments. By d...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.136.3.7867590
更新日期:1995-03-01 00:00:00
abstract::To examine the mechanism of estrogen's direct stimulation of steroidogenesis in the rabbit corpus luteum, we tested the hypothesis that the effect of estrogen on progestin production occurs at the site of processing of the precursor for pregnenolone (i.e. cholesterol) in the mitochondrion. For this purpose, we manipul...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-122-5-1948
更新日期:1988-05-01 00:00:00
abstract::The present study was undertaken to evaluate the efficacy of an estradiol-chemical delivery system (E2-CDS) for the brain vs. estradiol benzoate (E2-BNZ) in suppressing serum testosterone (T) and weights of the ventral prostate and seminal vesicle in male rats. Also, the role of serum T in the weight reduction of andr...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-129-2-726
更新日期:1991-08-01 00:00:00
abstract::A miniature thermoelectric probe was used to record continuously tissue blood flow in the anterior pituitary gland of anesthetized dogs. The output of the flow probe is linear in the range of tissue blood flow encountered in the pituitary. Probe placement required minimal tissue trauma, and zero blood flow was recorde...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-97-3-630
更新日期:1975-09-01 00:00:00
abstract::The estrogen-induced and -dependent Syrian hamster renal tumor is the most intensively studied model in estrogen carcinogenesis. Yet, it remains confounding that the kidney of this species behaves as an estrogen target tissue. As both reproductive and urinary systems arise from the same germinal ridge, we propose that...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.142.9.8355
更新日期:2001-09-01 00:00:00
abstract::A detailed understanding of the mechanism of action of estrogen on bones is still lacking. The present study was designed to examine possible modulation by 17 beta-estradiol (E2) on the effects of 1,25-dihydroxyvitamin D3 [1,25-(OH)2D3] and on vitamin D receptors (VDR) in the ROS 17/2.8 osteoblast-like cell line. Cell...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.130.5.1315250
更新日期:1992-05-01 00:00:00
abstract::The steroid sex hormone progesterone (P) induces the expression of a variety of genes through a signal transduction pathway mediated by the P receptor, a DNA-binding regulator of transcription. To identify genes and gene networks that are P dependent in the rhesus endometrium, we used a powerful technique employing su...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.134.3.8119170
更新日期:1994-03-01 00:00:00
abstract::3 beta-Hydroxy-5-ene-steroid dehydrogenase/delta 5-delta 4-isomerase (3 beta HSD) is a NAD(+)-dependent membrane-bound enzyme that catalyzes the oxidation of delta 5-3 beta-hydroxysteroids to delta 4-3-keto structures during adrenal, gonadal, and placental steroidogenesis. Enzyme activity is located in both microsomes...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.134.2.8299570
更新日期:1994-02-01 00:00:00
abstract::To examine the molecular genetic basis for the age-related increase in PRL secretion and decrease in LH production in the rat, we measured steady state levels of PRL and LH beta mRNA in pituitary homogenates and cell lysates from monolayer adenohypophyseal cultures. These mRNA levels were compared with the correspondi...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-126-2-773
更新日期:1990-02-01 00:00:00
abstract::In previous studies we demonstrated a much greater rate of glucose cycling (glucose-->glucose-6-P-->glucose) in islets from ob/ob mice than from lean litter mates. Cycling was further augmented by dexamethasone treatment. To determine whether these findings could be accounted for by increased islet glucose-6-phosphata...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.136.5.7720640
更新日期:1995-05-01 00:00:00
abstract::In mice, GH levels rise in response to short-term fasting or starvation (food restriction to 40% of ad libitum intake), similar to that which occurs in humans in response to fasting or anorexia. Recent studies using acyl-ghrelin knockout mice have suggested that the rise in GH during food restriction is essential to s...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2012-1849
更新日期:2013-01-01 00:00:00
abstract::Male and female Wistar-Furth rats bearing the pituitary tumor MtT/W15 had serum GH and PRL levels several hundred-fold higher, and immunoreactive somatomedin levels 3-fold higher, than those of controls. The presence of tumor appeared to have no effect on specific binding of radioiodinated bovine GH to liver microsoma...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-111-3-1020
更新日期:1982-09-01 00:00:00
abstract::The concentration of biologically active LH in rhesus monkey (Macaca mulatta) serum was measured by a highly sensitive bioassay based upon testosterone production by dispersed rat interstitial cells. The sensitivity of the in vitro bioassay was equal to or higher than that of radioimmunoassay, with detection limits of...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-100-6-1557
更新日期:1977-06-01 00:00:00
abstract::The adrenergic control of cAMP and 3',5'-cyclic GMP (cGMP) in dispersed adult rat pinealocytes was investigated. Norepinephrine treatment increased cAMP and cGMP content 60- and 400-fold, respectively; both alpha- and beta-adrenoceptors had to be activated for these responses to occur. Beta-Adrenergic stimulation alon...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-116-6-2167
更新日期:1985-06-01 00:00:00
abstract::Human proenkephalin generated by means of a recombinant vaccinia virus expression vector was used as the substrate for a putative processing enzyme obtained from bovine adrenal chromaffin granules. The adrenal enzyme successfully cleaved proenkephalin to generate low mol wt enkephalins as well as other enkephalin-cont...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-126-1-480
更新日期:1990-01-01 00:00:00
abstract::Estrogen-based hormone therapy (HT) in postmenopausal women may reduce the risk of Alzheimer's disease (AD), although HT remains controversial. One key concern with HT is the potential of adverse outcomes such as breast and uterine cancer. A promising strategy to maximize HT benefits and minimize HT risks is the use o...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2007-1346
更新日期:2008-05-01 00:00:00
abstract::Nonsteroidal anti-inflammatory drugs (NSAIDs) play potential roles in chemoprevention of colon cancer and others by inhibiting prostaglandin synthesis. In this report, we used LNCaP cells, an androgen-responsive human prostate carcinoma cell line, to study the effects of two NSAIDs, flufenamic acid (FA) and piroxicam ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.140.11.7246
更新日期:1999-11-01 00:00:00
abstract::Although acute leptin administration in the hypothalamus decreases food intake and increases peripheral energy metabolism, the peripheral actions of central chronic leptin administration are less understood. In this study, we investigated what effects chronic (7 d) intracerebroventricular (ICV) administration of lepti...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2011-1254
更新日期:2011-11-01 00:00:00