Abstract:
PURPOSE:Both HSV-1 and HSV-2 express LAT during viral latency. Previous studies indicated that deletion of either LAT impairs viral reactivation from latency, but that the HSV-1 LAT confers the ability to reactivate efficiently from trigeminal ganglia onto HSV-2. We sought to determine whether the HSV-2 LAT could function in the context of HSV-1. METHODS:A chimeric HSV-1 that expresses the HSV-2 LAT in place of both copies of its own LAT was constructed. A rescuant virus, in which the wild-type genotype was restored, was also constructed to demonstrate that any phenotypic differences between the mutant virus and wild-type virus were due to the introduced mutations rather than to inadvertent mutations elsewhere in the virus. All viruses were tested in vitro and in vivo (via inoculation of rabbit eyes and induction of reactivation via iontophoresis of epinephrine) to determine the ability of the HSV-2 LAT to substitute for the HSV-1 LAT during acute infection and reactivation from latency. RESULTS:Substitution of the HSV-2 LAT for the HSV-1 LAT had no effect on acute replication or on the ability of the virus to cause disease in the rabbit ocular model. The ability of the mutant virus to reactivate from latency was substantially impaired relative to wild-type HSV-1. CONCLUSIONS:The HSV-2 LAT cannot substitute for the HSV-1 LAT in promoting reactivation from HSV-1 latency from rabbit trigeminal ganglia. This is consistent with the LAT being the major determinant of site-specific HSV reactivation.
journal_name
Curr Eye Resjournal_title
Current eye researchauthors
Hill J,Patel A,Bhattacharjee P,Krause Pdoi
10.1076/ceyr.26.3.219.14896subject
Has Abstractpub_date
2003-03-01 00:00:00pages
219-24issue
3-4eissn
0271-3683issn
1460-2202journal_volume
26pub_type
杂志文章abstract:PURPOSE:Nuclear factor-kappa B (NF-κB) has been proposed as a therapeutic target for the treatment of cataracts. The authors investigated the relationship between nuclear factor of activated T cells 5 (NFAT5) and NF-κB in ultraviolet B (UVB)-irradiated human lens epithelial (HLE) cells. METHODS:Human lens epithelial B...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.1080/02713683.2016.1270327
更新日期:2017-07-01 00:00:00
abstract::The clinical impression that pre-existing diabetes exacerbates radiation injury to the retinal vasculature was studied in STZ diabetic rats. Half of 2 groups of streptozotocin (STZ)-induced diabetic rats and 1 group of normal animals had their right eyes irradiated with 1000 cGy of 90 KVP x-rays. The prevalence of ace...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713689309029227
更新日期:1993-11-01 00:00:00
abstract:PURPOSE:To investigate the effect of shaking on corneal endothelial preservation. METHODS:Thirty-six dog corneal buttons were obtained under standard eye bank procedure, after the animals had been sacrificed for cardiovascular experiments in the surgical department. They were equally divided into two groups. In Group ...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.1076/ceyr.16.11.1111.5111
更新日期:1997-11-01 00:00:00
abstract::A model system for retinal adhesion, consisting of interacting bovine pigment, epithelium (PE) cells and photoreceptor outer segments, was utilized to examine any adhesive role of the interphotoreceptor matrix. PE cells were dissociated by trypsin treatment of the eyecup, and were allowed to replenish their surface pr...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713688109001859
更新日期:1981-01-01 00:00:00
abstract:PURPOSE:This study was undertaken in order to investigate whether the production of vasodilatory prostaglandins is increased in the retinal vasculature of the diabetic rat. METHODS:Diabetes was induced in male Sprague-Dawley rats using streptozotocin. Diabetic rats were left uncontrolled for 3-4 weeks or were treated ...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.1076/ceyr.18.2.79.5386
更新日期:1999-02-01 00:00:00
abstract:PURPOSE:Choroidal neovascularization (CNV) is a major cause of vision loss in patients with age-related macular degeneration (AMD). Stromal cell-derived factor-1 (SDF-1)/CXC chemokine receptor 4 (CXCR4) plays a critical role in homing of bone marrow-derived cells (BMCs) to choroidal neovascularization (CNV). In this st...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713683.2011.593107
更新日期:2011-09-01 00:00:00
abstract::Purpose: To map the time course of changes in intraocular straylight of the human eye 1 year after initial lens wearing for orthokeratology treatment using the objective double-pass technique. Materials and Methods: A total of 35 subjects (19 males and 16 females) completed the study. The mean age was 11.46 ± 2.33 yea...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.1080/02713683.2018.1514056
更新日期:2019-01-01 00:00:00
abstract:PURPOSE:A retrospective study was developed to evaluate risk factors in adult-onset myopia. METHODS:Subjects included were 25 to 35 years old. There were 116 non-myopic subjects in the control group and 66 myopic subjects with first lens prescription at age 17 or later. Subjects received a questionnaire about academic...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.1076/ceyr.25.5.309.13494
更新日期:2002-11-01 00:00:00
abstract::Changes in the content of calcitonin gene-related polypeptide (CGRP) and tyrosine hydroxylase (TH) immunoreactivities in the anterior segment of the rat eye were assessed histochemically in animals subjected to sympathetic ganglionectomy or to chronic sensory denervation induced by neonatal capsaicin treatment. In the...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713688809033207
更新日期:1988-08-01 00:00:00
abstract::The normal aqueous humor contains a variety of soluble immunosuppressive factors, including transforming growth factor-beta, alpha-melanocyte stimulating hormone, and vasoactive intestinal peptide. These factors are largely the secretory products of parenchymal cells of the iris and ciliary body. TGF beta has recently...
journal_title:Current eye research
pub_type: 杂志文章,评审
doi:10.3109/02713689208999510
更新日期:1992-01-01 00:00:00
abstract:PURPOSE:Several procedures in cataract surgery carry the risk of high temperature increases in the capsular bag. The present study was undertaken to determine the shrinkage temperature of the human lens capsule and to investigate the effect of temperature on the mechanical behavior of the lens capsule. METHOD:Thermal-...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.1076/ceyr.17.5.470.5198
更新日期:1998-05-01 00:00:00
abstract:PURPOSE:Folic acid (FA) is an essential nutrient for normal embryonic development. FA deficiency (FAD) in maternal diet increases the risk of several defects among the progeny, especially, neural tube defects. The eye begins its development from the neural tube; however, the relationship between FAD and ocular developm...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.1080/02713683.2018.1545911
更新日期:2019-04-01 00:00:00
abstract::The mechanism of oxidative damage to the lens through intraocular photochemical generation of superoxide and its derivatization to other oxidants such as singlet oxygen, hydroxyl radical and hydrogen peroxide has been studied. Rat lenses when organ cultured aerobically in TC 199 containing additional amounts of ribofl...
journal_title:Current eye research
pub_type: 杂志文章,评审
doi:10.3109/02713688408997186
更新日期:1984-01-01 00:00:00
abstract::Cultured retinal glial cells from the rat are responsive to modulation by vasoactive intestinal peptide (VIP). VIP (1 X 10(-6) M) elevated the intracellular cyclic AMP concentration from the basal level of (4.4-11.1) p mole/mg protein to (354-440) p mole/mg protein in three minutes at 25 degrees C. The half-maximal co...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713688909000045
更新日期:1989-11-01 00:00:00
abstract::Activity of RNA polymerases I, II and III (distinguished using alpha-amanitin) and activity of DNA polymerases alpha and beta (distinguished using N-ethylmaleimide) were assayed for varying intervals and at varying substrate (UTP or dTTP) concentrations in the purified nuclear fraction from corneal epithelium of carba...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713688709025204
更新日期:1987-03-01 00:00:00
abstract:PURPOSE:Histidine is an indispensable amino acid with an imidazole ring and it is a precursor of histamine, carnosine, and anserine. Histidine has been proposed to act as a neurotransmitter or neuromodulator in mammalian central nervous system (CNS), including retina. In this study, we report histidine-like immunoreact...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.1076/ceyr.16.6.600.5084
更新日期:1997-06-01 00:00:00
abstract:PURPOSE:To compare serum levels of chemerin in type 2 diabetes mellitus (T2DM) with or without retinopathy, and to investigate the relationship between serum chemerin levels and diabetes retinopathy. MATERIALS AND METHODS:A total of 60 T2DM patients and 20 healthy subjects (control group) were enrolled in this study. ...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713683.2015.1004718
更新日期:2016-01-01 00:00:00
abstract::Purpose: To evaluate the effect of isolated oblique muscle weakening on horizontal deviation in patients with A- and V-pattern exotropia.Methods: The medical records of patients who underwent bilateral oblique muscle weakening for A- and V-pattern exotropia associated oblique muscle overaction between 2011 and 2018 we...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.1080/02713683.2019.1660795
更新日期:2020-02-01 00:00:00
abstract::The distribution of vasoactive intestinal polypeptide (VIP)- and neurotensin (NT)-like immunoreactivities in the chicken retina was investigated by immunohistochemistry. VIP- and NT-positive cells were found throughout the chicken retina. The majority of them were located in the proximal portion of the inner nuclear l...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713688109001736
更新日期:1981-01-01 00:00:00
abstract:PURPOSE:Increases in matrix proteins, such as type I collagen and fibronectin, are observed with aging in the retinal pigment epithelium (RPE) basement membrane. However, little is known about altered gene expression profiles of RPE associated with increases in matrix proteins. We investigated changes in gene expressio...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.1080/02713680701604749
更新日期:2007-10-01 00:00:00
abstract:PURPOSE:Compare the efficacy of treatment of pneumococcal keratitis with cholesterol, moxifloxacin, or a mixture of the two (moxifloxacin/cholesterol). MATERIALS AND METHODS:New Zealand white rabbits were injected intrastromally with 10(6) colony-forming units (CFU) of a clinical keratitis strain of Streptococcus pneu...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713683.2010.512114
更新日期:2010-12-01 00:00:00
abstract:PURPOSE:We have previously described a metabolic acidosis-induced retinopathy in the neonatal rat, similar to retinopathy of prematurity (ROP). We also have reported exacerbation of oxygen-induced retinopathy by postnatal growth retardation, produced by raising newborn rats in 'expanded' litters. In the present study, ...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.1076/ceyr.22.2.133.5531
更新日期:2001-02-01 00:00:00
abstract::Serum zinc and copper levels were analyzed in 26 retinitis pigmentosa patients and compared with serum zinc levels of a control group. Serum zinc levels were documented to be decreased with a mean concentration of 0.735 +/- 0.167 micrograms/ml (p less than 0.001). Serum copper levels, on the other hand, were found to ...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713688409011750
更新日期:1984-08-01 00:00:00
abstract:PURPOSE:To identify vitreous proteins that were differentially expressed in patients suffering from proliferative diabetic retinopathy with active neovascularization. METHODS:The vitreous samples of 15 active proliferative diabetic retinopathy patients were analyzed by two-dimensional gel electrophoresis and mass spec...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.1080/02713680600557030
更新日期:2006-03-01 00:00:00
abstract:PURPOSE:To evaluate choroidal thickness (CT) and retinal morphological changes in eyes with neovascular age-related macular degeneration (nAMD) following ranibizumab or aflibercept intravitreal treatment. MATERIALS AND METHODS:This was a prospective, observational, comparative study where 76 eyes of 76 consecutive pat...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.1080/02713683.2017.1405045
更新日期:2018-03-01 00:00:00
abstract:PURPOSE:To evaluate how patient satisfaction after cataract surgery is associated with postoperative visual acuity, visual functioning, dry eye signs and symptoms, health anxiety, and depressive symptoms. PATIENTS AND METHODS:Fifty-four patients (mean age: 68.02 years) were assessed 2 months after uneventful phacoemul...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.1080/02713683.2016.1262429
更新日期:2017-06-01 00:00:00
abstract:PURPOSE:The effects of several serotonergic agonists on aqueous humor formation (AHF), total outflow facility (OF) and intraocular pressure (IOP) were investigated in living cynomolgus monkeys. METHODS:We determined the effect of a single topical unilateral 300 microg or 3 mg dose of the 5-HT agonists serotonin, 5-car...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.1076/ceyr.23.2.120.5477
更新日期:2001-08-01 00:00:00
abstract::Recent reports from this laboratory indicate that dopamine (DA2) agonists can lower intraocular pressure (IOP) in rabbits, monkeys and rats. This communication suggests that the cat nictitating membrane (CNM) preparation is a useful model for localizing the site(s) and mechanism(s) of action of dopamine (DA2) agonists...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713688409007415
更新日期:1984-11-01 00:00:00
abstract:PURPOSE:Corneal autofluorescence is related to advanced glycation end products formed by glucose that reaches the cornea via the aqueous humour. The aim of the study was to examine the influence on autofluorescence of changes in permeability of the blood aqueous barrier. METHODS:Corneal autofluorescence was measured i...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.1076/ceyr.26.4.307.15433
更新日期:2003-05-01 00:00:00
abstract:UNLABELLED:Abstract Purpose of the Study: Protein tyrosine phosphatase σ (PTPσ) acts as a neuronal receptor for chondroitin sulfate proteoglycans (CSPGs). CSPGs have inhibitory effects on experience-dependent plasticity and usually form lattice-like cell coatings that surround the parvalbumin (PV) interneurons in the v...
journal_title:Current eye research
pub_type: 杂志文章
doi:10.3109/02713683.2013.803287
更新日期:2013-10-01 00:00:00