Abstract:
BACKGROUND:Ca2+ is known to be involved in a number of metastatic processes including motility and proliferation which can result in store-depletion of Ca2+. Up regulation of genes which contribute to store operated channel (SOC) activity may plausibly be necessary for these processes to take place efficiently. TRPC proteins constitute a family of conserved Ca2+-permeable channels that have been shown to contribute to SOC activity. RESULTS:In breast cancer biopsy tissues, TRPC3 and TRPC6 were the predominant TRPC genes expressed with TRPC3 and TRPC6 being significantly up regulated compared to normal breast tissue. In the lowly metastatic breast cancer cell line MCF-7, TRPC6 was the chief TRPC gene expressed while in the highly metastatic breast cancer cell line MDA-MB-231 both TRPC3 and TRPC6 were the predominant TRPC genes expressed. Western blotting, immunoconfocal analysis and immunoprecipitation experiments confirmed that the MDA-MB-231 cell line expressed both TRPC3 and TRPC6 protein with the majority of protein being intracellular. TRPC3 and TRPC6 were found to be in an immunoprecipitatble complex and co-localize within the cell. To demonstrate the potential of targeting TRP channels in breast cancer, hyperforin reportably a specific activator of TRPC6 significantly reduced the growth and viability of the breast cancer cell lines but had no effect on the non-cancerous breast cell line. Silencing of TRPC6 in MDA-MB-231 cells resulted in a significant reduction in cell growth but not viability. CONCLUSION:TRPC channels may be potential future targets for breast cancer diagnosis and therapy and deserve further investigation to evaluate their role in cancer cell physiology.
journal_name
Cancer Cell Intjournal_title
Cancer cell internationalauthors
Aydar E,Yeo S,Djamgoz M,Palmer Cdoi
10.1186/1475-2867-9-23subject
Has Abstractpub_date
2009-08-18 00:00:00pages
23issn
1475-2867pii
1475-2867-9-23journal_volume
9pub_type
杂志文章abstract:BACKGROUND:While some risk factors for breast cancer are well-known, the influence of other factors, particularly virus infection, remains unclear. Human cytomegalovirus (HCMV) is widespread in the general population, and both molecular and epidemiological evidence has indicated links between HCMV and breast cancer. Th...
journal_title:Cancer cell international
pub_type: 杂志文章
doi:10.1186/s12935-017-0399-5
更新日期:2017-02-13 00:00:00
abstract:Background:Although major driver gene have been identified, the complex molecular heterogeneity of renal cell cancer (RCC) remains unclear. Therefore, more relevant genes need to be identified to explain the pathogenesis of renal cancer. Methods:Microarray datasets GSE781, GSE6344, GSE53000 and GSE68417 were downloade...
journal_title:Cancer cell international
pub_type: 杂志文章
doi:10.1186/s12935-020-01405-6
更新日期:2020-07-21 00:00:00
abstract:Background:The crucial role of long non-coding RNAs (lncRNAs) has been certified in human cancers. The lncRNAs with abnormal expressions could act as tumor inhibitors or oncogenes in the advancement of tumors. LBX2-AS1 was once reported to accelerate esophageal squamous cell carcinoma. Nonetheless, its function in gast...
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pub_type: 杂志文章
doi:10.1186/s12935-020-01207-w
更新日期:2020-04-26 00:00:00
abstract:BACKGROUND:Endometrial cancer is an invasive gynecological cancer prevalent in the world. The pathogenesis of endometrial cancer is related to multiple levels of regulation, referring to oestrogen, tumor-suppressor gene (e.g. PTEN) or microRNAs (e.g. miR-23a and miR-29b). Metapristone is a hormone-related drug, which i...
journal_title:Cancer cell international
pub_type: 杂志文章
doi:10.1186/s12935-020-01682-1
更新日期:2021-01-07 00:00:00
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journal_title:Cancer cell international
pub_type: 杂志文章
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更新日期:2017-03-07 00:00:00
abstract:BACKGROUND:miR-222 has been reported to be overexpressed in colorectal cancer and it influences cancer cell proliferation, drug resistance and metastasis. However, the underlying molecular mechanism of miR-222 in colorectal cancer cell invasion and migration has not been thoroughly elucidated to date. METHODS:The cell...
journal_title:Cancer cell international
pub_type: 杂志文章
doi:10.1186/s12935-017-0447-1
更新日期:2017-08-29 00:00:00
abstract:BACKGROUND:Serum Deprivation Protein Response (SDPR) plays an important role in formation of pulmonary alveoli. However, the functions and values of SDPR in lung cancer remain unknown. We explored prognostic value, expression pattern, and biological function of SDPR in non-small cell lung cancer (NSCLC) and KRAS-mutant...
journal_title:Cancer cell international
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更新日期:2021-01-12 00:00:00
abstract::Osteosarcoma (OS) is one of the most prevalent malignant cancers with lower survival and poor overall prognosis mainly in children and adolescents. Identifying the molecular mechanisms and OS stem cells (OSCs) as new concepts involved in disease pathogenesis and progression may potentially lead to new therapeutic targ...
journal_title:Cancer cell international
pub_type: 杂志文章,评审
doi:10.1186/s12935-018-0654-4
更新日期:2018-10-16 00:00:00
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journal_title:Cancer cell international
pub_type: 杂志文章
doi:10.1186/1475-2867-8-15
更新日期:2008-11-03 00:00:00
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journal_title:Cancer cell international
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更新日期:2003-08-20 00:00:00
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journal_title:Cancer cell international
pub_type: 杂志文章
doi:10.1186/s12935-020-01332-6
更新日期:2020-06-26 00:00:00
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journal_title:Cancer cell international
pub_type: 杂志文章
doi:10.1186/1475-2867-14-20
更新日期:2014-03-03 00:00:00
abstract:Background:Programmed cell death 1 (PD-1) functions as an immune checkpoint in the process of anti-tumor immune response. The PD-1 blockade is now becoming a fundamental part in cancer immunotherapy. So it's essential to elicit the PD-1 related immune process in different types of cancer. Methods:The Cancer Genome Atl...
journal_title:Cancer cell international
pub_type: 杂志文章
doi:10.1186/s12935-018-0712-y
更新日期:2018-12-27 00:00:00
abstract::[This corrects the article DOI: 10.1186/s12935-020-01426-1.]. ...
journal_title:Cancer cell international
pub_type: 已发布勘误
doi:10.1186/s12935-020-01482-7
更新日期:2020-08-18 00:00:00
abstract:Background:Cholangiocarcinoma (CCA) is an invasive malignancy arising from biliary epithelial cells; it is the most common primary tumour of the bile tract and has a poor prognosis. The aim of this study was to screen prognostic biomarkers for CCA by integrated multiomics analysis. Methods:The GSE32225 dataset was der...
journal_title:Cancer cell international
pub_type: 杂志文章
doi:10.1186/s12935-020-01212-z
更新日期:2020-04-22 00:00:00
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pub_type: 杂志文章
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更新日期:2019-02-14 00:00:00
abstract:BACKGROUND:In past few years, long non-coding RNAs (lncRNAs) have been reported to play regulatory roles during cancer progression. LncRNA SNHG10 has been explored in several sorts of cancers. However, its detailed role and mechanism are still not well understood in glioma. METHODS:Expression levels of genes were eval...
journal_title:Cancer cell international
pub_type: 杂志文章
doi:10.1186/s12935-020-01649-2
更新日期:2020-12-09 00:00:00
abstract:Background:Colorectal cancer (CRC) is one of the most common malignant tumors in the world. Siah E3 ubiquitin protein ligase 1 (Siah1) has been identified as a tumor suppressor gene and plays an important role in the development of malignant tumors. However, the potential role and molecular mechanism of Siah1 in the de...
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pub_type: 杂志文章
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journal_title:Cancer cell international
pub_type: 杂志文章
doi:10.1186/s12935-020-1099-0
更新日期:2020-01-23 00:00:00
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pub_type: 杂志文章
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journal_title:Cancer cell international
pub_type: 杂志文章
doi:10.1186/s12935-019-0955-2
更新日期:2019-09-09 00:00:00
abstract::Amongst males, leukaemia is the most common cause of cancer-related death in individuals younger than 40 years of age whereas in female children and adolescents, leukaemia is the most common cause of cancer-related death. Chronic myeloid leukaemia (CML) is a chronic leukaemia of the haematopoietic stem cells affecting...
journal_title:Cancer cell international
pub_type: 杂志文章,评审
doi:10.1186/s12935-017-0460-4
更新日期:2017-10-30 00:00:00
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journal_title:Cancer cell international
pub_type: 杂志文章
doi:10.1186/s12935-015-0179-z
更新日期:2015-02-28 00:00:00
abstract:Background:Esophageal squamous cell carcinoma (ESCC) is one of the most prevalent malignancies and a major cause of cancer related death worldwide, especially in China. Cell lines are widely used disease models for basic medical research, however, well characterized ESCC cell models from China were seldom reported. Mis...
journal_title:Cancer cell international
pub_type: 杂志文章
doi:10.1186/s12935-020-01268-x
更新日期:2020-05-24 00:00:00
abstract::Cancer is a major public health problem as one of the leading causes of death worldwide. Deciphering the molecular regulation mechanisms of tumor progression can make way for tumor diagnosis and therapy. Paternally expressed gene 10 (PEG10), located on human chromosome 7q21.3, has turned out to be an oncogene implicat...
journal_title:Cancer cell international
pub_type: 杂志文章,评审
doi:10.1186/s12935-018-0610-3
更新日期:2018-08-13 00:00:00
abstract:Background:Vaccinia viruses have emerged as attractive therapeutic candidates for cancer treatment due to their inherent ability of tumor tropism and oncolytic property. Cytosine deaminase (CD), which is derived from bacteria or yeast, can convert a relatively nontoxic prodrug 5-fluorocytosine (5-FC) into the active an...
journal_title:Cancer cell international
pub_type: 杂志文章
doi:10.1186/s12935-020-01340-6
更新日期:2020-06-15 00:00:00
abstract:BACKGROUND:MicroRNAs are 22-24 nt non-coding RNAs that bind to the 3' UTR of target mRNAs, thereby inducing mRNA degradation or inhibiting mRNA translation. Due to their implication in the regulation of post-transcriptional processes, the role of miRNAs in hepatocellular carcinoma (HCC) has been extensively studied. Ho...
journal_title:Cancer cell international
pub_type: 杂志文章
doi:10.1186/s12935-017-0386-x
更新日期:2017-02-21 00:00:00
abstract:BACKGROUND:Mononuclear platinum anticancer agents hold a pivotal place in the treatment of many forms of cancers, however, there is a potential to improve response to evade resistance development and toxic side effects. BBR3464 is a promising trinuclear platinum anticancer agent, which is a polyamine mimic. The aim was...
journal_title:Cancer cell international
pub_type: 杂志文章
doi:10.1186/1475-2867-12-20
更新日期:2012-05-28 00:00:00
abstract:Background:Long noncoding RNAs (lncRNAs) play essential roles in tumor progression. However, the functions and targets of lncRNAs in neuroblastoma (NB) progression still remain to be determined. In this study, we aimed to investigate the effect of lncRNA DLX6 antisense RNA 1 (DLX6-AS1) on NB and the underlying mechanis...
journal_title:Cancer cell international
pub_type: 杂志文章
doi:10.1186/s12935-019-0968-x
更新日期:2019-11-27 00:00:00
abstract:Background:Pancreatic cancer (PC) remains one of the most aggressive cancers worldwide. However, genetic factors underlying PC susceptibility remain largely unclear. Long noncoding RNA (lncRNA) HOX transcript antisense RNA (HOTAIR) acts as an oncogene and its genetic variation has been linked to many cancers. However, ...
journal_title:Cancer cell international
pub_type: 杂志文章
doi:10.1186/s12935-019-0761-x
更新日期:2019-02-28 00:00:00