Abstract：
:Peptide nucleic acid (PNA) is a DNA mimic having a pseudopeptide backbone that makes it extremely stable in biological fluids. PNA binds complementary RNA and DNA with high affinity and specificity. These qualities make PNA a leading agent among "third generation" antisense and antigene agents. Unfortunately, fast progress in the exploration of PNA as an experimental and therapeutical regulator of gene expression has been hampered by the poor cellular uptake of PNA. However, a number of transfection protocols for PNA have now been established. These include microinjection, electroporation, co-transfection with DNA, conjugation to lipophilic moieties, conjugation to peptides, etc. Here we give a short introduction to the basic findings on PNA as an antisense and antigene agent in cell-free in vitro systems. This is followed by a comprehensive evaluation of the most interesting literature concerning cellular delivery and the intracellular effect of PNA. Also the current progress as regards using PNA as co-factor in DNA delivery is reviewed.
journal_name
Adv Drug Deliv Revjournal_title
Advanced drug delivery reviewsauthors
Koppelhus U,Nielsen PEdoi
10.1016/s0169-409x(02)00182-5subject
Has Abstractpub_date
2003-02-10 00:00:00pages
267-80issue
2eissn
0169-409Xissn
1872-8294pii
S0169409X02001825journal_volume
55pub_type
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