Abstract：
BACKGROUND:Aggrecan, the large aggregating proteoglycan, is a major component of the cartilage extracellular matrix. The C-terminal globular domain (G3) of the aggrecan molecule exhibits a pattern of alternative splicing that involves three exons. Each corresponds to a subdomain, epidermal growth factor 1(EGF1), EGF2, and a short complement repeat (SCR). MATERIALS AND METHODS:In the present study, we compared aggrecan mRNA splicing variants in osteochondrogenic tumor cells and normal cartilage using RT-PCR. RESULTS:Normal cartilage expressed two mRNA isoforms, both containing the SCR exon, but with or without the EGF1 exon. Benign cartilaginous tumors showed the same splicing pattern as normal cartilage. However, malignant chondrosarcomas expressed additional splicing isoforms, one of which lacked all three alternative exons and another with EGF2 and SCR exons. The human chondrosarcoma cell line (Ch-1 cells) expressed further isoforms, one containing either EGF1 or EGF2 exon only, and another with all the variant exons. CONCLUSION:These results imply that a change in the pattern of alternative aggrecan mRNA splicing is associated with malignant transformation of chondrocytes.
journal_name
Anticancer Resjournal_title
Anticancer researchauthors
Matsui Y,Araki N,Tsuboi H,Tsumaki N,Nakata K,Kawabata H,Eyre DR,Yoshikawa Hsubject
Has Abstractpub_date
2002-11-01 00:00:00pages
4169-72issue
6Ceissn
0250-7005issn
1791-7530journal_volume
22pub_type
杂志文章abstract：BACKGROUND:Cancer-related acute disseminated intravascular coagulation (DIC) is uncommon, but it is a severe complication resulting in a very dismal prognosis. Choosing the appropriate chemotherapy agents to treat the underlying cancer and stop the acute DIC process effectively, while avoiding chemotherapy-induced myel...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2010-07-01 00:00:00
abstract：:The antitumor effect of a polyamine biosynthetic pathway inhibitor methylglyoxal bis(cyclopentylamidinohydrazone) (MGBCP) on human osteosarcoma cell lines such as KHOS-240S, MG-63 and G-292 cells, and its effect in combination with anticancer drugs such as methotrexate (MTX), adriamycin (ADM) and 4-hydroperoxyfosfamid...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：1995-05-01 00:00:00
abstract：BACKGROUND:Multidrug resistance (MDR) is one of the major obstacles limiting the efficacy of cancer chemotherapy. Through screening a series of natural products, we have previously identified six naturally occurring bisbenzylisoquinoline alkaloids (BBIs) that possess potent activity to reverse P-glycoprotein (gp)-media...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2005-05-01 00:00:00
abstract：:To investigate the specific transduction of a suicide gene into human small cell lung carcinoma (SCLC) cells, we explored the promoter region of the neuron specific enolase (NSE) gene as a tumor-specific promoter. In Northern blot analysis, NSE mRNA was expressed more abundantly in the SBC3 human SCLC cell line than i...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2001-01-01 00:00:00
abstract：BACKGROUND:The safety and advantages of perioperative autologous blood transfusion (ABT) were evaluated on hepatectomy for hepatocellular carcinoma (HCC). MATERIALS AND METHODS:Blood samples were obtained and stored from 30 patients with HCC. HCC cells were investigated by the presence of AFPmRNA using RT-PCR after st...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2001-09-01 00:00:00
abstract：:The triterpenoid fraction (100 and 200 mg/kg) of the fruit bodies of Ganoderma lucidum inhibited primary solid-tumor growth in the spleen, liver metastasis and secondary metastatic tumor growth in the liver in intrasplenic Lewis lung carcinoma (LLC)-implanted mice. In addition, the triterpenoid fraction (800 microgram...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2002-11-01 00:00:00
abstract：BACKGROUND:The antitumor growth and antimetastatic actions of celecoxib [a selective cyclooxygenase-2 (COX-2) inhibitor] were investigated in a metastatic murine mammary cancer model. MATERIALS AND METHODS:Mice bearing mammary tumors, developed after inoculation of syngeneic BALBIc mice with a mammary carcinoma cell l...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2006-11-01 00:00:00
abstract：:P-glycoprotein has been identified in mast cells stabilized in culture as well as in rat peritoneal mast cells, and is primarily concentrated on the granular membrane. This study aimed to define the role of this protein in the transport and accumulation of doxorubicin in mast cell granules and in its histamine releasi...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：1999-03-01 00:00:00
abstract：:The histoculture drug sensitivity assay (HDRA) has been demonstrated to have high predictability for resistance, sensitivity, and survival for gastrointestinal cancer (Clin Cancer Res 1: 305-311, 1995; Clin Cancer Res 1: 1537-1543, 1995). In this report, we evaluated the clinical usefulness of the HDRA in ovarian canc...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2000-05-01 00:00:00
abstract：AIM:We examined whether the perioperative systemic inflammation score (SIS), which describes systemic inflammation and/or malnutrition, affected the tumor recurrence and survival in advanced gastric cancer patients. PATIENTS AND METHODS:The study retrospectively analyzed 160 patients with stage II/III gastric cancer w...
journal_title：Anticancer research
pub_type： 杂志文章
doi：10.21873/anticanres.14095
更新日期：2020-03-01 00:00:00
abstract：:CD5 antigen is present on all normal alpha beta T cells and some B cells. Human NK cells do not usually express CD5 antigen, but we found a novel subset of CD5LOW (low density of CD5) positive (CD5LOW+) natural killer cells (NK cells) in patients with multiple myeloma (MM) and plasmacytoma. To detect CD5LOW+NK cells, ...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：1994-03-01 00:00:00
abstract：:The role of combination chemotherapy regimens in the management of ovarian cancer patients with tumors resistant to platinum compounds has not yet been defined. This multicenter prospective phase II study evaluated the activity and toxicity of the docetaxel-plus-irinotecan combination in ovarian cancer patients whose ...
journal_title：Anticancer research
pub_type： 临床试验,杂志文章,多中心研究
doi：
更新日期：2005-09-01 00:00:00
abstract：BACKGROUND/AIM:As a result of activation of transcription factors engaged in epithelial-mesenchymal transition (EMT), such as Twist, inhibition of epithelial markers and an increased expression of mesenchymal markers are observed. One of the specific markers of cancer-associated fibroblasts is podoplanin (PDPN) - a muc...
journal_title：Anticancer research
pub_type： 杂志文章
doi：10.21873/anticanres.11978
更新日期：2017-10-01 00:00:00
abstract：:The expression of epidermal growth factor (EGF) and its receptor (EGF-R) was examined immunohistochemically in 60 primary and 26 metastatic lesions of pancreatic carcinoma. EGF was stained mainly in the cytoplasm of tumor cells, and EGF-R was stained mainly on the surface of cells. The expression rates of EGF and EGF-...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：1997-09-01 00:00:00
abstract：BACKGROUND/AIM:Fibroblast growth factor (FGF), vascular endothelial growth factor, and hepatocyte growth factor play a critical role in the pathogenesis of hepatocellular carcinoma (HCC). MATERIALS AND METHODS:We assessed nine single nucleotide polymorphisms (SNPs) in the FGF1, FGF2, FGF receptor (FGFR)-2, Flt-1, and ...
journal_title：Anticancer research
pub_type： 杂志文章
doi：10.21873/anticanres.13337
更新日期：2019-04-01 00:00:00
abstract：:MOv18, a non-internalizing monoclonal antibody (MAb) with restricted tumor specificity, was conjugated to ricin toxin (RT). According to their ability to bind to galactose residues of Sepharose 6B, the immunoconjugates were fractionated into Bound and Unbound MOv18-RT. The two conjugates could be distinguished by SDS-...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：1991-01-01 00:00:00
abstract：BACKGROUND:Although p21 is an important component that regulates cell-cycle progression, no consensus has been reached about its clinicopathological significance in esophageal squamous cell carcinoma (ESCC). In the present study, we investigated its prognostic significance and correlation with cyclin-D1 (CCND1) express...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2013-10-01 00:00:00
abstract：BACKGROUND:Shortened, constitutively active androgen receptor (AR) isoforms have been characterized and linked to tumor progression and chemoresistance in prostate cancer (PCa). We examined the regulation of shortened AR isoforms by a newly-identified AR regulatory signaling pathway involving heat-shock protein HSPB1 a...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2013-11-01 00:00:00
abstract：UNLABELLED:Reduced serum-zinc concentrations are well known as typical laboratory characteristics in advanced head and neck cancer. Our aim was to follow the development of this phenomenon during the disease. PATIENTS AND METHODS:A total of 21 patients were included in this pilot-study (1 female, 20 male). The median ...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2007-07-01 00:00:00
abstract：BACKGROUND/AIM:5-Aminolevulinic acid (5-ALA), a precursor of protoporphyrin IX (PpIX), is now used for photodynamic therapy (PDT) of pre-cancers of the skin and photodynamic diagnosis (PDD) of brain tumors. Sonodynamic therapy (SDT) of cancers with ultrasound has been studied using 5-ALA as a sonosensitizer. In this ar...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2016-07-01 00:00:00
abstract：:The development and maintenance of testicular interstitial cell (IC) tumors under the conditions of low LH levels were examined in aged Fischer 344 rats, and the enzyme activities for androgen synthesis in the tumors were estimated. Sixty-week old rats received injections of an LH-RH agonist (LH-RHa) for 28 weeks or w...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：1990-03-01 00:00:00
abstract：:Adjuvant radioimmunotherapy (RIT) for human colonic cancer was performed in a nude rat model of experimental liver metastases. Thirty-three rats were injected intraportally through a mesenteric vein with 5 x 10(6) cells from the human colonic cancer cell line LS174T. Within half an hour, 20 MBq (n = 2), 75 MBq (n = 5)...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：1998-03-01 00:00:00
abstract：:In the colon of F344 rats treated with 2 x 15 mg/kg body weight of azoxymethane (AOM), the density (number of lesions/cm2/rat) of flat aberrant crypt foci (ACF) was 13-fold higher (p < 0.05) in the surface area of mucosa immediately adjacent to lymphoid follicles compared with the density of these lesions in the rest ...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2006-05-01 00:00:00
abstract：:Thirty-four patients of an Italian population affected by neuroblastoma (NB) were evaluated at diagnosis for multidrug resistance gene (MDR1) and N-myc oncogene amplification. No patients showed MDR1 amplification, while extra copies of the N-myc gene were found in 9 out of 34 patients (26%). N-myc amplification was c...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：1990-07-01 00:00:00
abstract：BACKGROUND:Inconsistent reporting in published meta-analyses compared to registered protocol are poorly understood. The aim of the study was to assess inconsistencies between registered protocols and published reports among oncology drug meta-analyses. MATERIALS AND METHODS:A cross-sectional study was performed includ...
journal_title：Anticancer research
pub_type： 杂志文章
doi：10.21873/anticanres.11928
更新日期：2017-09-01 00:00:00
abstract：:Small cell carcinomas of the gastrointestinal tract are rare and clinically aggressive tumors. A case is presented of a 70 year-old woman who presented with small bowel obstruction and was found to have a cecal mass. She underwent right hemicolectomy, and histopathology showed a small cell carcinoma arising in the bac...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2013-04-01 00:00:00
abstract：:We examined the influence of a regularly moderate endurance training program on qualitative and quantitative alterations of monocytes from 24 breast cancer patients. At the beginning, after 5 weeks and 7 months of bicycle training we analyzed the composition of leucocytes and phagocytotic activity of monocytes using t...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：1995-01-01 00:00:00
abstract：BACKGROUND:5-Aminolevulinic acid (5-ALA) has already been applied clinically as a photosensitizer. In this study, sonodynamically induced selective antitumour effect of 5-ALA for deep-seated lesions was evaluated. MATERIALS AND METHODS:First, normal rat brains were sonicated via a transducer placed on the dural surfac...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2011-07-01 00:00:00
abstract：BACKGROUND:The potential of MTp53 knockout by oligodesoxyribonucleotide phosphothioates (ODNs) to affect proliferation, apoptosis and chemosensitivity in undifferentiated thyroid cancer (UTC) cells with a recessive MTp53 mutation was evaluated. MATERIALS AND METHODS:Transient transfections with ODNs complementary to p...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2006-03-01 00:00:00
abstract：:Four patients with colorectal cancer after pelvic irradiation for gynaecological malignancies are reported. The interval between irradiation and the diagnosis of colorectal cancer was 15-28 years. Histological findings of radiation proctocolitis adjacent to the cancer were observed in all specimens. Ki-67 immunohistol...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：1997-01-01 00:00:00
