Abstract:
:The release of cytochrome c from the intermembrane space of mitochondria into the cytosol is one of the critical events in apoptotic cell death. In the present study, it is shown that release of cytochrome c and apoptosis induced by tumor necrosis factor alpha (TNF) in HeLa cells can be inhibited by (i) overexpression of an oncoprotein Bcl-2, (ii) Cyclosporin A, an inhibitor of the mitochondrial permeability transition pore (PTP) or (iii) oligomycin, an inhibitor of H+- ATP-synthase. Staurosporine-induced apoptosis is sensitive to Bcl-2 but insensitive to Cyclosporin A and oligomycin. The effect of oligomycin is not due to changes in mitochondrial membrane potential or to inhibition of ATP synthesis/hydrolysis since (a) uncouplers (CCCP, DNP) which discharge the membrane potential fail to abolish the protective action of oligomycin and (b) aurovertin B (another inhibitor of H+-ATP-synthase, affecting its F1 component) do not affect apoptosis. A role of oligomycin-sensitive F0 component of H+-ATP-synthase in the TNF-induced PTP opening and apoptosis is suggested.
journal_name
Oncogenejournal_title
Oncogeneauthors
Shchepina LA,Pletjushkina OY,Avetisyan AV,Bakeeva LE,Fetisova EK,Izyumov DS,Saprunova VB,Vyssokikh MY,Chernyak BV,Skulachev VPdoi
10.1038/sj.onc.1206053subject
Has Abstractpub_date
2002-11-21 00:00:00pages
8149-57issue
53eissn
0950-9232issn
1476-5594journal_volume
21pub_type
杂志文章相关文献
ONCOGENE文献大全abstract::A flow cytometric assay was developed to examine the expression of the cellular myc oncogene in relation to cell cycle in individual cells. C-myc-oncoprotein was detected by indirect immunofluorescence using a purified sheep polyclonal antibody, anti-human-myc. Specific binding of anti-human-myc was measured by flow c...
journal_title:Oncogene
pub_type: 杂志文章
doi:
更新日期:1988-10-01 00:00:00
abstract::In contrast to other cell cycle inhibitors, the tumor suppressor p16Ink4a is not detectable or expressed at very low levels in embryonic and adult mouse tissues, and therefore it has often been considered as a specialized checkpoint protein that does not participate in the control of normal cell cycle progression. How...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/sj.onc.1209437
更新日期:2006-07-06 00:00:00
abstract::The carboxyl terminus of c-Myb contains a negative regulatory domain that is absent in the v-Myb oncoprotein, but conserved among all the known Myb proteins of animals. This domain inhibits transcriptional activation by c-Myb in animal cells, but not in budding yeast, suggesting that additional protein(s) present in a...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/sj.onc.1204048
更新日期:2001-01-04 00:00:00
abstract::Epstein-Barr virus (EBV)-encoded Latent Membrane Protein 2A (LMP2A) is an EBV latency-associated protein regularly expressed in nasopharyngeal carcinoma (NPC). In B cells, LMP2A activity resembles that of a constitutively activated antigen receptor, which recruits the Syk tyrosine kinase to activate a set of downstrea...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/onc.2014.380
更新日期:2015-08-20 00:00:00
abstract::Two hepatocarcinoma cell lines, the Hepa-1 wild-type (c1c7) and the beta-subunit mutated (c4) lacking hypoxia-inducible factor-1 (HIF-1) activity, were differentially susceptible to apoptosis by hepatocyte growth factor (HGF). The c4 cells were 40% apoptotic 48 h after HGF treatment. On the contrary, the wild-type c1c...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/sj.onc.1206519
更新日期:2003-06-26 00:00:00
abstract::Although apoptosis can be induced by the enforced expression of exogenously introduced c-myc genes, it is not clear whether overexpression resulting from the amplification of the resident c-myc gene in tumor cells is sufficient to induce apoptosis. We have investigated the relationship between c-myc gene amplification...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/sj.onc.1202309
更新日期:1999-01-14 00:00:00
abstract::Protein kinase CK2 is a ubiquitous and pleiotropic Ser/Thr protein kinase involved in cell growth and transformation. Here we report the identification by yeast interaction trap of a CK2 interacting protein, UBC3B, which is highly homologous to the E2 ubiquitin conjugating enzyme UBC3/CDC34. UBC3B complements the yeas...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/sj.onc.1205574
更新日期:2002-06-06 00:00:00
abstract::AP-1 transcription factors play a critical role in signal transduction pathways in many cells. We have investigated the role of AP-1 in controlling proliferative signals in breast cells, and have previously shown that AP-1 complexes are activated by peptide and steroid growth factors in both normal and malignant breas...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/sj.onc.1205883
更新日期:2002-10-31 00:00:00
abstract::Signature abnormalities in the cell cycle and apoptotic pathway have been identified in mantle cell lymphoma (MCL), affording the opportunity to develop targeted therapies. In this study, we tested a novel class of kinase inhibitors, styryl sulfones, which differ from prior cell cycle inhibitors in that they are not r...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/sj.onc.1210350
更新日期:2007-08-16 00:00:00
abstract::PTEN (phosphatase and tensin homolog deleted on chromosome 10) tumor suppressor regulates a variety of cellular processes including cell proliferation, growth, migration and death. This master regulator itself is also under deliberative regulation. Although the evidence for PTEN regulation and its significance in norm...
journal_title:Oncogene
pub_type: 杂志文章,评审
doi:10.1038/onc.2008.242
更新日期:2008-09-18 00:00:00
abstract::Common fragile sites are specific genomic loci that form constrictions and gaps on metaphase chromosomes under conditions that slow, but do not arrest, DNA replication. These sites have been shown to have a role in various chromosomal rearrangements in tumors. Different DNA damage response proteins were shown to regul...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/sj.onc.1210849
更新日期:2008-04-03 00:00:00
abstract::Eukaryotic translation can be initiated either by a cap-dependent mechanism or by internal ribosome entry, a process by which ribosomes are directly recruited to structured regions of mRNA upstream of the initiation codon. Here we report the finding of an internal ribosome entry site (IRES) in the untranslated region ...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/sj.onc.1206149
更新日期:2003-01-30 00:00:00
abstract::Epithelial-mesenchymal transition (EMT) has pivotal roles during embryonic development and carcinoma progression. Members of the Snai1 family of zinc finger transcription factors are central mediators of EMT and induce EMT in part by directly repressing epithelial markers such as E-cadherin, a gatekeeper of the epithe...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/onc.2010.234
更新日期:2010-09-02 00:00:00
abstract::Hox genes encode DNA-binding proteins that are deployed in overlapping domains along various body axes during embryonic development. This sequential activation of Hox genes in temporal and spatial mode, the Hox code, is critical for the proper positioning of segmented structures along those axes, which include the ver...
journal_title:Oncogene
pub_type: 杂志文章,评审
doi:10.1038/onc.2010.484
更新日期:2011-01-27 00:00:00
abstract::Poor-prognosis oestrogen receptor-positive breast cancer is characterised by the presence of high-level focal amplifications. We utilised a focused small interfering RNA screen in 14 breast cancer cell lines to define genes that were pathogenic in three genomic regions focally amplified in oestrogen receptor-positive ...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/onc.2013.202
更新日期:2014-05-08 00:00:00
abstract::p53 is involved in several DNA repair pathways. Some of these require the specific transactivation of p53-dependent genes and others involve direct interactions between the p53 protein and DNA repair associated proteins. Previously, we have shown that p53 acts directly in Base Excision Repair (BER) when assayed under ...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/sj.onc.1204120
更新日期:2001-02-01 00:00:00
abstract::Epstein-Barr virus (EBV) is associated with tumours of both lymphoid and epithelial origin. Whilst a role for EBV latent genes in the development of these malignancies is accepted, it is also possible that viral proteins involved in EBV replication may influence the oncogenic process. BHRF1 is an immediate early prote...
journal_title:Oncogene
pub_type: 杂志文章
doi:
更新日期:1995-01-05 00:00:00
abstract::The transcription factor Ets-1 regulates the expression of several angiogenic and extracellular matrix remodeling factors, and might be implicated in disease progression of breast cancer. In the present study, the prognostic value of Ets-1 expression was assessed by quantitative real-time fluorescence RT-PCR in 123 sp...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/sj.onc.1206040
更新日期:2002-12-05 00:00:00
abstract::Intraductal papillary mucinous neoplasm (IPMN), the most common pancreatic cystic neoplasm, is known to progress to invasive ductal adenocarcinoma. IPMNs commonly harbor activating somatic mutations in GNAS and KRAS, primarily GNAS(R201H) and KRAS(G12D). GNAS encodes the stimulatory G-protein α subunit (Gsα) that medi...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/onc.2015.294
更新日期:2016-05-05 00:00:00
abstract::Acute promyelocytic leukemia (APL) is characterized by a block in myeloid cell differentiation. As a result of a chromosomal translocation in these patients, the promyelocytic leukemia protein PML is disrupted as are the nuclear bodies it forms. Disruption of PML and PML nuclear bodies in APL is linked to a loss of gr...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/sj.onc.1203201
更新日期:1999-11-25 00:00:00
abstract::The tumor suppressor protein, p53, plays a critical role as a transcriptional activator of downstream target genes involved in the cellular response to DNA damaging agents. We examined the cell cycle checkpoint response of human mammary epithelial cells (HMEC) and their isogenic fibroblast counterparts to ionizing (IR...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/sj.onc.1202977
更新日期:1999-10-14 00:00:00
abstract::A candidate gene (WT1) has recently been described for the 11p13 tumour-suppressor gene involved in the development of Wilms' tumour. This gene encodes a zinc finger protein which can bind to a specific DNA sequence. We have found a 226 base deletion in the mRNA from a unilateral Wilms' tumour, which would cause a fra...
journal_title:Oncogene
pub_type: 杂志文章
doi:
更新日期:1992-04-01 00:00:00
abstract::The RAS/RAF mitogen-activated protein kinase pathway (MAPK) is highly active in many tumor types including the majority of high-grade gliomas and expression of activated RAS or RAF in neural progenitor cells combined with either AKT activation or Ink4a/Arf loss leads to the development of high-grade gliomas in vivo. T...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/onc.2010.513
更新日期:2011-03-17 00:00:00
abstract::Frequent allelic losses of chromosome 3p in lung cancer have been reported in a number of studies, and we previously demonstrated that 3p21.3 is one of the common regions of deletion in lung cancers and renal cell carcinomas. To further define a region containing the putative tumor suppressor gene, we performed Southe...
journal_title:Oncogene
pub_type: 杂志文章
doi:
更新日期:1993-02-01 00:00:00
abstract::The human trk oncogene (originally identified in a colon carcinoma) was activated by a genetic rearrangement which resulted in replacement of the extracellular ligand-binding domain of the proto-trk transmembrane receptor by non-muscle tropomyosin sequences. The product of the trk oncogene, a protein of 70 kDa (p70trk...
journal_title:Oncogene
pub_type: 杂志文章
doi:
更新日期:1991-12-01 00:00:00
abstract::DNA double-strand breaks (DSBs), the most hazardous DNA lesions, may result in genomic instability, a hallmark of cancer cells. The main DSB repair pathways are non-homologous end joining (NHEJ) and homologous recombination (HR). In mammalian cells, NHEJ, which can lead to inaccurate repair, predominates. HR repair (H...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/onc.2011.516
更新日期:2012-07-26 00:00:00
abstract::Juvenile myelomonocytic leukemia (JMML) is a malignant hematopoietic disorder whose proliferative component is a result of RAS pathway deregulation caused by somatic mutation in the RAS or PTPN11 oncogenes or in patients with underlying neurofibromatosis type 1 (NF-1), by loss of NF1 gene function. To search for poten...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/sj.onc.1210361
更新日期:2007-08-23 00:00:00
abstract::The p53 protein is known to play a central role in mediating G1 arrest or apoptosis in response to ionizing radiation in some cell types. It has been proposed that the link between p53 and induction of apoptosis is provided in part by p53-mediated upregulation of BAX. In this study, we used the human SW626 ovarian can...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/sj.onc.1201132
更新日期:1997-06-12 00:00:00
abstract::ETS is a family of transcription factors that contain a highly conserved ETS DNA binding domain. Various members of the ETS family are expressed in cells of hematopoietic lineage. ETS-1, ETS-2 and ERGB/FLI-1 are expressed at high levels in T-lymphocytes. HIV-1 infects T-cells and it has been shown that its LTR contain...
journal_title:Oncogene
pub_type: 杂志文章
doi:
更新日期:1996-01-04 00:00:00
abstract::Nicotinic acetylcholine receptors (nAChRs) are ligand-gated ion channels that modulate key physiological processes ranging from neurotransmission to cancer signaling. These receptors are activated by the neurotransmitter, acetylcholine, and the tobacco alkaloid, nicotine. Recently, the gene cluster encoding the alpha3...
journal_title:Oncogene
pub_type: 杂志文章,评审
doi:10.1038/onc.2010.256
更新日期:2010-09-02 00:00:00