Abstract:
:Protein kinase CK2 is a ubiquitous and pleiotropic Ser/Thr protein kinase involved in cell growth and transformation. Here we report the identification by yeast interaction trap of a CK2 interacting protein, UBC3B, which is highly homologous to the E2 ubiquitin conjugating enzyme UBC3/CDC34. UBC3B complements the yeast cdc34-2 cell cycle arrest mutant in S. cerevisiae and transfers ubiquitin to a target substrate in vitro. UBC3B is specifically phosphorylated by CK2 in vitro and in vivo. We mapped by deletions and site directed mutagenesis the phosphorylation site to a serine residue within the C-terminal domain in position 233 of UBC3B and in the corresponding serine residue of UBC3. Following CK2-dependent phosphorylation both UBC3B and UBC3 bind to the F-box protein beta-TrCP, the substrate recognition subunit of an SCF (Skp1, Cul1, F-box) ubiquitin ligase. Furthermore, we observed that co-transfection of CK2alpha' together with UBC3B, but not with UBC3DeltaC, enhances the degradation of beta-catenin. Taken together these data suggest that CK2-dependent phosphorylation of UBC3 and UBC3B functions by regulating beta-TrCP substrate recognition.
journal_name
Oncogenejournal_title
Oncogeneauthors
Semplici F,Meggio F,Pinna LA,Oliviero Sdoi
10.1038/sj.onc.1205574subject
Has Abstractpub_date
2002-06-06 00:00:00pages
3978-87issue
25eissn
0950-9232issn
1476-5594journal_volume
21pub_type
杂志文章相关文献
ONCOGENE文献大全abstract::Constitutive activation of the signal transducer and activator of transcription 3 (Stat3) and mutation of the p53 are both commonly detected in human prostate cancer cells. We sought to investigate whether there is functional regulation of Stat3 by wild-type (wt) p53. Our results demonstrate that expression of wt p53 ...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/sj.onc.1205426
更新日期:2002-05-02 00:00:00
abstract::Activation of peroxisome proliferator-activated receptor (PPAR)-gamma by the thiazolidinedione (TZD) class of antidiabetic drugs elicits growth inhibition in a variety of malignant tumors. We clarified the effects of TZDs on growth of human non-small cell lung carcinoma (NSCLC) cells that express endogenous PPAR-gamma...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/sj.onc.1205279
更新日期:2002-03-28 00:00:00
abstract::DAL-1 (differentially expressed in adenocarcinoma of the lung)/4.1B is a tumor suppressor gene on human chromosome 18p11.3 whose expression is lost in >50% of primary non-small-cell lung carcinomas. Based on sequence similarity, DAL-1/4.1B has been assigned to the Protein 4.1 superfamily whose members interact with pl...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/sj.onc.1208057
更新日期:2004-10-14 00:00:00
abstract::Human telomerase is a unique reverse transcriptase that is expressed in multiple cancers, but not in the vast majority of normal cells. The enzyme is responsible for telomere protection and maintenance, and supports the proliferative immortality of cancer cells. Thus, it has been proposed that the specific inhibition ...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/sj.onc.1205064
更新日期:2002-01-21 00:00:00
abstract::Transformed cells express high levels of non-telomeric reverse-transcriptase (RT) activity of retrotransposon and endogenous retrovirus origin. We previously reported that RT inhibition, either pharmacological or through transient silencing of RT-encoding LINE-1 (L1) elements by RNA interference (RNAi), reduced prolif...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/sj.onc.1210214
更新日期:2007-06-21 00:00:00
abstract::The Interleukin-1beta converting enzyme (ICE) family of proteins, homologs of the C elegans cell death gene product CED-3, play important roles in controlling vertebrate programmed cell death. Because inhibition of apoptosis may be an essential step in tumorigenesis, we investigated the interaction of the simian virus...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/sj.onc.1200943
更新日期:1997-03-13 00:00:00
abstract::Dysregulation of cellular signaling pathways can lead to colon cancer. However, research on the key signaling effectors or regulators in colon carcinogenesis is limited. Casein kinase-2 interacting protein-1 (CKIP-1; also known as PLEKHO1) is crucial during adult bone formation and is a promising drug target for osteo...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/onc.2013.340
更新日期:2014-07-10 00:00:00
abstract::The p14ARF tumor suppressor is a key regulator of cellular proliferation, frequently inactivated in human cancer. The mechanisms that regulate alternative reading frame (ARF) turnover have been obscure for long time, being ARF a relatively stable protein. Recently, it has been described that its degradation depends, a...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/sj.onc.1210313
更新日期:2007-08-02 00:00:00
abstract::Bloom syndrome and ataxia-telangiectasia are autosomal recessive human disorders characterized by immunodeficiency, genome instability and predisposition to develop cancer. Recent data reveal that the products of these two genes, BLM and ATM, interact and function together in recognizing abnormal DNA structures. To in...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/sj.onc.1207276
更新日期:2004-02-26 00:00:00
abstract::Replication origins are 'licensed' for a single initiation event by loading Mcm2-7 complexes during late mitosis and G1. Licensing is blocked at other cell cycle stages by the activity of cyclin-dependent kinases and a small protein called geminin. Here, we describe the effects of over-expressing a non-degradable form...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/sj.onc.1205910
更新日期:2002-09-26 00:00:00
abstract::Nerve growth factor (NGF)- and ras-induced neuronal differentiation of PC12 cells is accompanied by expression of transin, a secreted metalloproteinase. Retinoic acid (RA) is known to exert important effects on neural cell proliferation and differentiation. In this study we have analysed different PC12 sublines which ...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/sj.onc.1200997
更新日期:1997-04-10 00:00:00
abstract::Oncogenes in DNAs from human tumor cell lines have been detected by a new transformation assay. Cellular DNAs are transfected into NIH3T3 murine fibroblasts, and transformed cells are selected by maintaining cell cultures in a defined medium lacking platelet-derived or fibroblast growth factors. DNAs from eight of 17 ...
journal_title:Oncogene
pub_type: 杂志文章
doi:
更新日期:1987-01-01 00:00:00
abstract::Mice bred to carry germline Rb and p53 null alleles are associated with a tumor spectrum that overlaps with the inherited multiple endocrine neoplasia-1 (MEN1) and MEN2 syndromes in humans, including medullary thyroid cancer (MTC). To study the genetic basis for these tumors, we microdissected MTC specimens or obtaine...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/sj.onc.1202381
更新日期:1998-09-24 00:00:00
abstract::We have recently developed an allele titration assay (ATA) to assess the sensitivity and influence of normal cell admixture in loss of heterozygosity (LOH) studies based on CA-repeat. The assay showed that these studies are biased by the size-dependent differential sensitivity of allele detection. Based on these data,...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/sj.onc.1203449
更新日期:2000-03-09 00:00:00
abstract::The cellular response to ionizing radiation (IR) includes the induction of apoptosis. The p300/CBP proteins possess histone acetyltransferase activity and function as transcriptional coactivators of p53. We have prepared cells deficient in p300 or CBP to define the roles of these proteins in the cellular response to D...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/sj.onc.1202930
更新日期:1999-10-07 00:00:00
abstract::The onset of human lung cancer occurs through sequential mutations in oncogenes and tumor suppressor genes. Mutations in K-Ras play a prominent role in human non-small cell lung cancer. We have developed a mouse lung tumor model in which K-Ras can be sporadically activated through Cre-lox mediated somatic recombinatio...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/sj.onc.1204837
更新日期:2001-10-04 00:00:00
abstract::We report here the nucleic acid sequence and deduced amino acid sequence of a cDNA for TIS7, a gene induced by mitogens in 3T3 cells and by nerve growth factor in PC12 pheochromocytoma cells. A fragment of the TIS7 gene has previously been cloned from murine fibroblast cells infected with Newcastle Disease Virus. The ...
journal_title:Oncogene
pub_type: 杂志文章
doi:
更新日期:1989-10-01 00:00:00
abstract::A search for protein tyrosine kinases expressed during murine cardiogenesis resulted in the isolation of a novel tyrosine kinase, designated tek, which maps to mouse chromosome 4 between the brown and pmv-23 loci. The deduced amino acid sequence of tek predicts that it encodes a putative receptor tyrosine kinase that ...
journal_title:Oncogene
pub_type: 杂志文章
doi:
更新日期:1992-08-01 00:00:00
abstract::TCR-mediated activation of T cell hybridomas induces programmed cell death by a Fas-dependent pathway. We now show that costimulation of 2B4 cells, in the absence or presence of transgenic Bcl-2, with anti-CD3 epsilon and forskolin, an activator of cAMP signaling, resulted in antagonism of Fas-dependent activation-ind...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/sj.onc.1201088
更新日期:1997-05-22 00:00:00
abstract::Adhesion to the extracellular matrix (ECM) is critical for epithelial tissue homeostasis and function. ECM detachment induces metabolic stress and programmed cell death via anoikis. ECM-detached mammary epithelial cells are able to rapidly activate autophagy allowing for survival and an opportunity for re-attachment. ...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/onc.2012.512
更新日期:2013-10-10 00:00:00
abstract::Somatic mitochondrial DNA (mtDNA) mutations have been increasingly observed in primary human cancers. As each cell contains many mitochondria with multiple copies of mtDNA, it is possible that wild-type and mutant mtDNA can co-exist in a state called heteroplasmy. During cell division, mitochondria are randomly distri...
journal_title:Oncogene
pub_type: 杂志文章,评审
doi:10.1038/sj.onc.1209604
更新日期:2006-08-07 00:00:00
abstract::Telomerase expression is the hallmark of tumor cells in which this ribonucleoprotein complex preserves chromosome integrity by maintaining telomere length and thereby prevents cell death. However, recent data support a role of the combination of p53 and telomerase inactivation in initiating genetic instability that pr...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/sj.onc.1206468
更新日期:2003-06-12 00:00:00
abstract::ErbB-2 is an orphan receptor that belongs to a family of tyrosine kinase receptors for either epidermal growth factor (EGF) or Neu differentiation factor (NDF/neuregulin). Because overexpression of the erbB-2 proto-oncogene is frequently associated with an aggressive clinical course of certain human adenocarcinomas, t...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/sj.onc.1201029
更新日期:1997-05-01 00:00:00
abstract::The protein encoded by the MAX gene is a member of the class of basic region-helix-loop-helix-zipper proteins and has been demonstrated to associate with N-, L-, and c-Myc proteins both in vitro and in vivo. Heterodimers formed between c-Myc and Max proteins have been shown to possess sequence-specific DNA-binding act...
journal_title:Oncogene
pub_type: 杂志文章
doi:
更新日期:1992-06-01 00:00:00
abstract::Epidemiological studies have demonstrated a close association of type 2 diabetes and hepatocellular carcinoma (HCC). Exenatide (Ex-4), a potent diabetes drug targeting glucagon-like peptide-1 receptor (GLP-1R), is protective against non-alcoholic fatty liver disease (NAFLD). However, the Ex-4 function and GLP-1R statu...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/onc.2017.38
更新日期:2017-07-20 00:00:00
abstract::Mdm2 is the major negative regulator of p53 tumor-suppressor activity. This oncoprotein is overexpressed in many human tumors that retain the wild-type p53 allele. As such, targeted inhibition of Mdm2 is being considered as a therapeutic anticancer strategy. The N-terminal hydrophobic pocket of Mdm2 binds to p53 and t...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/onc.2011.625
更新日期:2012-11-08 00:00:00
abstract::PTEN, encoding a lipid phosphatase, is a tumor suppressor gene and is mutated in various types of cancers. It is reported to regulate G1 to S phase transition of the cell cycle by influencing the expression, protein stability and subcellular location of cyclin D1. Here, we provide evidence that PTEN modulates the tran...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/sj.onc.1210033
更新日期:2007-04-12 00:00:00
abstract::Latent membrane protein 1 (LMP1), an oncoprotein encoded by Epstein-Barr virus (EBV), is an integral membrane protein, which acts like a constitutively active receptor. LMP1 is critical for some facet of EBV's induction and maintenance of proliferation of infected B cells. It, in part, mimics signaling by the CD40 rec...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/sj.onc.1208367
更新日期:2005-03-03 00:00:00
abstract::Midkine is a heparin-binding growth factor, originally reported as the product of a retinoic acid-responsive gene during embryogenesis, but currently viewed as a multifaceted factor contributing to both normal tissue homeostasis and disease development. Midkine is abnormally expressed at high levels in various human m...
journal_title:Oncogene
pub_type: 杂志文章,评审
doi:10.1038/s41388-019-1124-8
更新日期:2020-03-01 00:00:00
abstract::The Myc-deregulating chromosomal T(12;15)(Igh-Myc) translocation, the hallmark mutation of inflammation- and interleukin 6-dependent mouse plasmacytoma (PCT), is the premier model of cancer-associated chromosomal translocations because it is the only translocation in mice that occurs spontaneously (B lymphocyte lineag...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/onc.2010.150
更新日期:2010-07-15 00:00:00