Abstract:
:We performed parallel array comparative genomic hybridization and array expression analysis of the 12p11-p12 amplicon in human testicular seminomas and an ovarian carcinoma cell line using an expressed sequence tags (ESTs) array spotted with 8254 ESTs. The data were normalized using a robust statistical modeling and the significance inferred from the local SD. We identified two ESTs within the chromosomal amplicon that were amplified and overexpressed in > or =75-100 percent of analyzed tumors with the 12p11-p12 amplicon. These sequences, belonging to coding regions of two novel genes designated here as GCT1 and GCT2, were broadly expressed in a panel of human tissues, including testis and ovary. GCT1 and GCT2 were overexpressed in 92 and 71 percent, respectively, of a panel of seminomas tested. Combined array comparative genomic hybridization and array expression analysis is a valid approach for gene discovery in large chromosomal amplicons.
journal_name
Cancer Resjournal_title
Cancer researchauthors
Bourdon V,Naef F,Rao PH,Reuter V,Mok SC,Bosl GJ,Koul S,Murty VV,Kucherlapati RS,Chaganti RSsubject
Has Abstractpub_date
2002-11-01 00:00:00pages
6218-23issue
21eissn
0008-5472issn
1538-7445journal_volume
62pub_type
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