Transcriptome analysis of all two-component regulatory system mutants of Escherichia coli K-12.

abstract：:The monocistronic transcript of rpsO undergoes an endonucleolytic cleavage downstream of the coding sequence, which removes the hairpin of the transcription terminator and initiates the rapid degradation of the message. We demonstrate here that the two rne-dependent cleavages, on both sides of the transcription termin...

journal_title：Molecular microbiology

authors： Braun F,Hajnsdorf E,Régnier P

更新日期：1996-03-01 00:00:00

abstract：:Among players in neurobiology, ion channels are the demigods that underlie all our senses, behaviour and intelligence. In animals, these 'gated pores' detect ligands, voltage, heat or stretch forces and emit electric or ionic signals. Patch clamp and genome sequencing now show that nearly all microbes also have these ...

journal_title：Molecular microbiology

authors： Kung C,Blount P

更新日期：2004-07-01 00:00:00

abstract：:Strains of Escherichia coli have been produced which express very high levels of the tRNA(1Leu) isoacceptor. This was accomplished by transforming cells with plasmids containing the leuV operon which encodes three copies of the tRNA(1Leu) gene. Most transformants grew very slowly and exhibited a 15-fold increase in ce...

journal_title：Molecular microbiology

authors： Wahab SZ,Rowley KO,Holmes WM

更新日期：1993-01-01 00:00:00

abstract：:The diffusible factor synthase XanB2, originally identified in Xanthomonas campestris pv. campestris (Xcc), is highly conserved across a wide range of bacterial species, but its substrate and catalytic mechanism have not yet been investigated. Here, we show that XanB2 is a unique bifunctional chorismatase that hydroly...

journal_title：Molecular microbiology

authors： Zhou L,Wang JY,Wu J,Wang J,Poplawsky A,Lin S,Zhu B,Chang C,Zhou T,Zhang LH,He YW

更新日期：2013-01-01 00:00:00

abstract：:Schizosaccharomyces pombe Rho1p regulates (1,3)beta-d-glucan synthesis and is required for cell integrity maintenance and actin cytoskeleton organization, but nothing is known about the regulation of this protein. At least nine different S. pombe genes code for proteins predicted to act as Rho GTPase-activating protei...

journal_title：Molecular microbiology

authors： Calonge TM,Arellano M,Coll PM,Perez P

更新日期：2003-01-01 00:00:00

abstract：:The analysis of completed archaeal genome sequences led to the identification of a set of approximately 10-20 genes whose protein products were inferred to be involved in chromosomal DNA replication. Until recently, however, little was known of the biochemical properties of these proteins. Here, I review recent progre...

journal_title：Molecular microbiology

authors： MacNeill SA

更新日期：2001-05-01 00:00:00

abstract：:The pathogenic fungus Candida albicans commonly causes mucosal surface infections. In immunocompromised patients, C. albicans may penetrate into deeper tissue, enter the bloodstream and disseminate within the host causing life-threatening systemic infections. In order to elucidate how C. albicans responds to the chall...

journal_title：Molecular microbiology

authors： Fradin C,Kretschmar M,Nichterlein T,Gaillardin C,d'Enfert C,Hube B

更新日期：2003-03-01 00:00:00

abstract：:When they infect Escherichia coli cells, the filamentous phages IF1 and fd first interact with a pilus and then target TolA as their common receptor. They use the domains N2 and N1 of their gene-3-proteins (G3P) for these interactions but differ in the mechanism of infection. In G3P of phage IF1, N1 and N2 are indepen...

journal_title：Molecular microbiology

authors： Lorenz SH,Schmid FX

更新日期：2011-05-01 00:00:00

abstract：:The nucleotide sequence of the ugp genes of Escherichia coli K-12, which encode a phosphate-limitation inducible uptake system for sn-glycerol-3-phosphate and glycerophosphoryl diesters, was determined. The genetic organization of the operon differed from previously published results. A single promoter, containing a p...

journal_title：Molecular microbiology

authors： Overduin P,Boos W,Tommassen J

更新日期：1988-11-01 00:00:00

abstract：:Previous work demonstrated that selection for Escherichia coli mutants with low antibiotic resistance frequently resulted in co-selection of lon mutations and that lon(-) mutants evolved higher-level resistance faster than a lon(+) strain. Here we show that lon mutation causes a very low multidrug resistance by induci...

journal_title：Molecular microbiology

authors： Nicoloff H,Andersson DI

更新日期：2013-12-01 00:00:00

abstract：:Listeriolysin O (LLO) binds to cholesterol-containing membranes in which it oligomerizes to form pores. Preincubation of the toxin with cholesterol is known to inhibit haemolysis, whereas the oxidized form of cholesterol has no inhibitory effect. Using immunoblot analyses and flow cytometry we demonstrate that preincu...

journal_title：Molecular microbiology

authors： Jacobs T,Darji A,Frahm N,Rohde M,Wehland J,Chakraborty T,Weiss S

更新日期：1998-06-01 00:00:00

abstract：:Two pathogenic species within the genus Neisseria cause the diseases gonorrhoea and meningitis. While vaccines are available to protect against four N. meningitidis serogroups, there is currently no commercial vaccine to protect against serogroup B or against N. gonorrhoeae. Moreover, the available vaccines have signi...

journal_title：Molecular microbiology

authors： Noinaj N,Buchanan SK,Cornelissen CN

更新日期：2012-10-01 00:00:00

abstract：:Multimer formation reduces plasmid copy number and is an established cause of segregational instability. Nevertheless, it is difficult to rationalize observations that low levels of dimers can cause severe instability, if we assume they are distributed evenly in cell populations. We report here that dimer distribution...

journal_title：Molecular microbiology

authors： Summers DK,Beton CW,Withers HL

更新日期：1993-06-01 00:00:00

abstract：:Borrelia burgdorferi, the Lyme disease agent, has double-stranded linear plasmids with covalently closed ends. DNA at the ends, or telomeres, of two linear plasmids of B. burgdorferi strain B31 was examined. Telomeric sequences from both ends of a 16 kb linear plasmid and from one end of a 49 kb linear plasmid were cl...

journal_title：Molecular microbiology

authors： Hinnebusch J,Bergström S,Barbour AG

更新日期：1990-05-01 00:00:00

abstract：:The response regulator/histidine kinase pair LiaRS of Bacillus subtilis, together with its membrane-bound inhibitor protein LiaF, constitutes an envelope stress-sensing module that is conserved in Firmicutes bacteria. LiaR positively autoregulates the expression of the liaIH-liaGFSR operon from a strictly LiaR-depende...

journal_title：Molecular microbiology

authors： Schrecke K,Jordan S,Mascher T

更新日期：2013-02-01 00:00:00

abstract：:The carboxyl-terminal region of diphtheria toxin (DT) has been analysed in order to determine regions of receptor recognition. Biochemical cleavage of the toxin with hydroxylamine (HA) was used to generate the peptides HA9DT (residues 454-535), HA6DT (residues 482-535), and HA3DT (residues 454-481). Characterization o...

journal_title：Molecular microbiology

authors： Rolf JM,Eidels L

更新日期：1993-02-01 00:00:00

abstract：:DNA double-strand break repair can be accomplished by homologous recombination when a sister chromatid or a homologous chromosome is available. However, the study of sister chromatid double-strand break repair in prokaryotes is complicated by the difficulty in targeting a break to only one copy of two essentially iden...

journal_title：Molecular microbiology

authors： Cromie GA,Leach DR

更新日期：2001-08-01 00:00:00

abstract：:The identification, isolation and characterization of a new Aspergillus nidulans positive-acting gene metR, which encodes a transcriptional activator of sulphur metabolism, is reported. metR mutants are tight auxotrophs requiring methionine or homocysteine for growth. Mutations in the metR gene are epistatic to mutati...

journal_title：Molecular microbiology

authors： Natorff R,Sieńko M,Brzywczy J,Paszewski A

更新日期：2003-08-01 00:00:00

abstract：:A gene bank of Azorhizobium caulinodans DNA constructed in the bacteriophage lambda GEM11 was screened with Rhizobium meliloti fixL and fixJ genes as probes. One positive recombinant phage, ORS lambda L, was isolated. The nucleotide sequence of a 3.7 kb fragment was established. Two open reading frames of 1512bp and 6...

journal_title：Molecular microbiology

authors： Kaminski PA,Elmerich C

更新日期：1991-03-01 00:00:00

abstract：:Escherichia coli cells use two distinct sensory circuits during chemotaxis towards carbohydrates. One circuit requires the phosphoenolpyruvate-dependent phosphotransferase system (PTS) and is independent of any specific chemoreceptor, whereas the other uses a chemoreceptor-dependent sensory mechanism analogous to that...

journal_title：Molecular microbiology

authors： Kristich CJ,Glekas GD,Ordal GW

更新日期：2003-03-01 00:00:00

abstract：:The cyanobacterium Synechocystis sp. PCC 6803 expresses four different FtsH protease subunits (FtsH1-4) that assemble into specific homo- and heterocomplexes. The FtsH2/FtsH3 complex is involved in photoprotection but the physiological roles of the other complexes, notably the essential FtsH1/FtsH3 complex, remain unc...

journal_title：Molecular microbiology

authors： Krynická V,Tichý M,Krafl J,Yu J,Kaňa R,Boehm M,Nixon PJ,Komenda J

更新日期：2014-11-01 00:00:00

abstract：:Biofilm formation of Staphylococcus epidermidis on smooth polymer surfaces has been shown to be mediated by the ica operon. Upon activation of this operon, a polysaccharide intercellular adhesin (PIA) is synthesized that supports bacterial cell-to-cell contacts and triggers the production of thick, multilayered biofil...

journal_title：Molecular microbiology

authors： Ziebuhr W,Krimmer V,Rachid S,Lössner I,Götz F,Hacker J

更新日期：1999-04-01 00:00:00

abstract：:In the yeast Saccharomyces cerevisiae starvation for nitrogen on a glucose-containing medium causes entrance into G0 and downregulation of all targets of the PKA pathway. Re-addition of a nitrogen source in the presence of glucose causes rapid activation of trehalase and other PKA targets. Trehalase activation upon am...

journal_title：Molecular microbiology

authors： Van Nuland A,Vandormael P,Donaton M,Alenquer M,Lourenço A,Quintino E,Versele M,Thevelein JM

更新日期：2006-03-01 00:00:00

abstract：:Transcription of the ansB gene, encoding L-asparaginase II, is positively regulated by cAMP receptor protein (CRP) and by the product of the fnr gene, the FNR protein. These global regulatory proteins mediate the expression of ansB in Escherichia coli in response to carbon source and to anaerobiosis, respectively, and...

journal_title：Molecular microbiology

authors： Jennings MP,Beacham IR

更新日期：1993-07-01 00:00:00

abstract：:Agrobacterium tumefaciens transforms plants by transferring DNA to the plant cell nucleus. The VirB membrane proteins are postulated to form a pore for the transport of the DNA across the bacterial membranes. Immunofluorescence and immunoelectron microscopy were used to study the transport pore complex. Three likely c...

journal_title：Molecular microbiology

authors： Kumar RB,Xie YH,Das A

更新日期：2000-05-01 00:00:00

abstract：:PspF bound to the psp enhancer activates E sigma54 holoenzyme-dependent transcription of the Escherichia coli phage-shock protein (psp) operon and autogenously represses its own sigma70-dependent transcription, thereby keeping its concentration at a low level. It has been demonstrated previously that integration host ...

journal_title：Molecular microbiology

authors： Jovanovic G,Model P

更新日期：1997-08-01 00:00:00

abstract：:Type VI secretion is critical for Vibrio cholerae to successfully combat phagocytic eukaryotes and to survive in the presence of competing bacterial species. V. cholerae type VI secretion system genes are encoded in one large and two small clusters. In V. cholerae, type VI secretion is controlled by quorum sensing, th...

journal_title：Molecular microbiology

authors： Shao Y,Bassler BL

更新日期：2014-06-01 00:00:00

abstract：:Helicobacter pylori is anomalous among non nitrogen-fixing bacteria in containing an incomplete NIF system for Fe-S cluster assembly comprising two essential proteins, NifS (cysteine desulfurase) and NifU (scaffold protein). Although nifU deletion strains cannot be obtained via the conventional gene replacement, a Nif...

journal_title：Molecular microbiology

authors： Benoit SL,Holland AA,Johnson MK,Maier RJ

更新日期：2018-05-01 00:00:00

abstract：:We have cloned the rpoD gene encoding the principal sigma (sigma) factor of Helicobacter pylori. The deduced amino acid sequence reveals a predicted polypeptide of 676 residues that has amino acid homology with the principal sigma factors of a number of divergent prokaryotes. We have designated this factor sigma80. Am...

journal_title：Molecular microbiology

authors： Beier D,Spohn G,Rappuoli R,Scarlato V

更新日期：1998-10-01 00:00:00

abstract：:In Caulobacter crescentus the partitioning proteins ParA and ParB operate a molecular switch that couples chromosome partitioning to cytokinesis. Homologues of these proteins have been shown to be important for the stable inheritance of F-plasmids and the prophage form of bacteriophage P1. In C. crescentus, ParB binds...

journal_title：Molecular microbiology

authors： Figge RM,Easter J,Gober JW

更新日期：2003-03-01 00:00:00