Abstract:
:Patch-clamp recordings were carried out in the inside-out configuration in human keratinocytes of the cell line HaCaT. Patch pipettes were filled with 150 mM KCl, 1 mM CaCl(2) and 10 mM HEPES. In symmetrical KCl solutions, single channel currents from a large conductance channel (about 170 pS) were measured. Replacement of 120 mM KCl by K-aspartate had only a minor influence on the single channel conductance and on the reversal potential. In intracellular solution in which K(+) has been replaced by Na(+) or NMDG(+), the reversal potential shifted to > + 40 mV indicating K(+) as the main charge carrier. The channels were neither dependent on intracellular Ca(2+) (between 0.8 nM and 10 micro M), ATP (at 0 and 1 mM) nor Mg(2+) (at 0 and 0.5 mM). The mean current showed an outward rectification that can be mainly attributed to the voltage dependence of the open probability. The channels displayed bursting kinetics with a mean open time of about 2 ms and closed times of about 0.2, 2 and 20 ms. The mean open probability was usually low (0.05) but increased occasionally (0.6) mainly due to a lower probability of long closings. We conclude that these K(+) channels contribute to the resting potential of human keratinocytes which may control the Ca(2+) influx and thereby their proliferation and differentiation.
journal_name
Exp Dermatoljournal_title
Experimental dermatologyauthors
Nguyen VH,Markwardt Fdoi
10.1034/j.1600-0625.2002.110405.xsubject
Has Abstractpub_date
2002-08-01 00:00:00pages
319-26issue
4eissn
0906-6705issn
1600-0625pii
exd110405journal_volume
11pub_type
杂志文章abstract::Plasmatic proteasome (p-proteasome) also called circulating proteasome has recently been described as a tumor marker. We investigated the diagnostic and prognostic accuracies of p-proteasome levels in a melanoma population classified according to the American Joint Committee on Cancer staging system. Using an ELISA te...
journal_title:Experimental dermatology
pub_type: 杂志文章
doi:10.1111/j.1600-0625.2010.01151.x
更新日期:2010-12-01 00:00:00
abstract::Placental extracts have been widely used as skin lightening agents in the Japanese cosmetic market. Here, we show that placental extracts contain factors that can decrease or increase melanin synthesis by normal human melanocytes in vitro in possible association with mitochondrial respiration. When normal human melano...
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journal_title:Experimental dermatology
pub_type: 杂志文章
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更新日期:2009-02-01 00:00:00
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journal_title:Experimental dermatology
pub_type: 杂志文章
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更新日期:1997-08-01 00:00:00
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journal_title:Experimental dermatology
pub_type: 杂志文章
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pub_type: 杂志文章
doi:10.1111/j.1600-0625.2009.00899.x
更新日期:2009-12-01 00:00:00
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pub_type: 杂志文章
doi:10.1111/exd.13797
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journal_title:Experimental dermatology
pub_type: 杂志文章
doi:10.1111/exd.12111
更新日期:2013-04-01 00:00:00
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pub_type: 杂志文章
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journal_title:Experimental dermatology
pub_type: 杂志文章
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journal_title:Experimental dermatology
pub_type: 杂志文章
doi:10.1111/exd.14111
更新日期:2020-05-09 00:00:00
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pub_type: 杂志文章
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journal_title:Experimental dermatology
pub_type: 临床试验,杂志文章,随机对照试验
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pub_type: 杂志文章,评审
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journal_title:Experimental dermatology
pub_type: 杂志文章
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pub_type: 杂志文章
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journal_title:Experimental dermatology
pub_type: 杂志文章
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pub_type: 杂志文章
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更新日期:2005-01-01 00:00:00
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pub_type: 杂志文章
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pub_type: 评论,杂志文章
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journal_title:Experimental dermatology
pub_type: 杂志文章
doi:10.1111/exd.12086
更新日期:2013-02-01 00:00:00
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pub_type: 杂志文章
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