Abstract:
:The correlation between infant leukemia and in utero exposure to topoisomerase II (topo-II) inhibitor has been clarified. We examined the in vitro effect of topo-II inhibitor (etoposide) on cleavage of the MLL gene in cord and peripheral blood mononuclear cells (MNCs). Southern blot analysis showed cleavage of the MLL gene in peripheral blood MNCs of infants when the MNCs were exposed to etoposide. MNCs were incubated with etoposide at various concentrations (1 to 50 microM), and a ligation-mediated polymerase chain reaction (LM-PCR) was used to detect double strand breaks (DSBs) of DNA in intron 8 of the MLL breakpoint cluster region. PCR products obtained with LM-PCR were subcloned and sequenced to identify the breakpoint in the MLL gene. The PCR products indicated DSBs of the MLL gene were obtained without any difference in the incidence between 3 different samples (cord and peripheral blood from infants and children). Sequencing analysis showed that the DSBs occurred on the telomeric side of intron 8 and near exon 9. There was no evidence that the cord blood was more susceptible to MLL DNA breakage by topo-II inhibitor than were other cells. Instability of the partner gene during the fetal period could be associated with the pathogenesis of infant leukemia.
journal_name
Int J Hematoljournal_title
International journal of hematologyauthors
Ishii E,Eguchi M,Eguchi-Ishimae M,Yoshida N,Oda M,Zaitsu M,Fujita I,Miyazaki S,Hamasaki Y,Mizutani Sdoi
10.1007/BF02982722subject
Has Abstractpub_date
2002-07-01 00:00:00pages
74-9issue
1eissn
0925-5710issn
1865-3774journal_volume
76pub_type
杂志文章abstract::Integrins, a family of noncovalently associated alpha beta heterodimeric adhesion receptors, are involved in a variety of pathological and physiological processes. The importance of integrins is evident in the severe pathogenic consequences of their congenital deficiencies: Glanzmann thrombasthenia (GT) and leukocyte ...
journal_title:International journal of hematology
pub_type: 杂志文章,评审
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journal_title:International journal of hematology
pub_type: 临床试验,杂志文章
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journal_title:International journal of hematology
pub_type: 杂志文章,评审
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journal_title:International journal of hematology
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journal_title:International journal of hematology
pub_type: 杂志文章,评审
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journal_title:International journal of hematology
pub_type: 杂志文章
doi:
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journal_title:International journal of hematology
pub_type: 杂志文章,多中心研究
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pub_type: 杂志文章,meta分析,评审
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journal_title:International journal of hematology
pub_type: 临床试验,杂志文章
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更新日期:1998-12-01 00:00:00
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journal_title:International journal of hematology
pub_type: 杂志文章
doi:10.1007/BF02983557
更新日期:2003-11-01 00:00:00
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journal_title:International journal of hematology
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journal_title:International journal of hematology
pub_type: 杂志文章
doi:
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pub_type: 杂志文章
doi:10.1007/s12185-009-0334-1
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journal_title:International journal of hematology
pub_type: 杂志文章
doi:10.1007/s12185-007-0017-8
更新日期:2008-01-01 00:00:00
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journal_title:International journal of hematology
pub_type: 杂志文章
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doi:
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journal_title:International journal of hematology
pub_type: 杂志文章
doi:10.1007/BF02982554
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journal_title:International journal of hematology
pub_type: 杂志文章
doi:
更新日期:1993-10-01 00:00:00
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pub_type: 杂志文章
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abstract::The clinical importance of the isolation of nontuberculous mycobacteria (NTM) from respiratory specimens of stem cell transplant (SCT) recipients is not clear. We investigated the characteristics and clinical impact of NTM isolation in this population. Medical records of adult patients who underwent SCT at the blood a...
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journal_title:International journal of hematology
pub_type: 杂志文章
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journal_title:International journal of hematology
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