Photoeradication of Helicobacter pylori using 5-aminolevulinic acid: preliminary human studies.

Abstract:

BACKGROUND AND OBJECTIVES:Helicobacter pylori (HP) is an endemic pathogenic bacterium causing gastritis and gastroduodenal ulceration in humans and is linked to the development of gastric malignancies. These first human in vivo studies investigated the photoeradication of HP using laser and white light. STUDY DESIGN/MATERIALS AND METHODS:In 13 HP-positive volunteers, a zone of gastric antrum was irradiated with laser (410 nm, 50 J/cm(2)) or endoscopic white light (10 J/cm(2)) 45 minutes after oral 5-aminolevulinic acid (5-ALA) 20 mg/kg. HP-eradication was assessed by biopsy urease test and HP-culture from irradiated and control zones 5 minutes, 4 and 48 hours post-irradiation. RESULTS:A maximum eradication effect was achieved at 4 hours post-irradiation when 85% of biopsies in the monochromatic and 66% in the white light exposed zones, and 58 and 33% in the respective control zones were HP-negative. CONCLUSIONS:HP numbers were greatly reduced following exposure to 5-ALA and either laser or white light in vivo. Photoeradication appears feasible, but further light dosimetry and the development of convenient application methods is required.

journal_name

Lasers Surg Med

authors

Wilder-Smith CH,Wilder-Smith P,Grosjean P,van den Bergh H,Woodtli A,Monnier P,Dorta G,Meister F,Wagnières G

doi

10.1002/lsm.10066

subject

Has Abstract

pub_date

2002-01-01 00:00:00

pages

18-22

issue

1

eissn

0196-8092

issn

1096-9101

journal_volume

31

pub_type

临床试验,杂志文章
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