Abstract:
BACKGROUND AND OBJECTIVE:High-power laser has recently become a physical stimulus for bone regeneration. Little is known about how high-power laser irradiation affects osteoblast differentiation. This study investigated osteoblast responses to high-power laser and combined irradiation with BMP-2 treatment. STUDY DESIGN/MATERIALS AND METHODS:MC3T3-E1 pre-osteoblasts were exposed to laser irradiation, 100 ng/ml BMP-2 or both. Cells were irradiated with a Q-switched, pulsed neodymium-doped yttrium aluminum garnet (Nd:YAG) laser, with a 1,064 nm wavelength and 0.75 W output power under 1.5, 3, or 5 J/cm(2) energy densities. Cell proliferation was evaluated using tetrazolium salt, WST-8. To determine the effect of these treatments on in vitro osteogenesis, we examined alkaline phosphatase (ALP) activity, mineral deposition, and expression of genes associated with osteogenesis. Quantitative real time PCR or ELISA was used to examine cytokine expression. In each experiment, either non-irradiated or BMP-2 (100 ng/ml)-treated cells were used as controls. RESULTS:High-power, low-level, Nd:YAG laser irradiation significantly increased ALP activity, when combined with BMP-2 or not. Cell proliferation declined in the irradiation and combined irradiation/BMP-2 groups. Interestingly, Nd:YAG laser stimulation resulted in significant induction of endogenous BMP-2 protein and gene expression. The increased expression of upstream regulators cbfa1 by Nd:YAG laser alone was comparable to exogenous BMP-2 treatment (100 ng/ml). Combined laser/BMP-2 treatment was synergistic in the expression of some genes (IGF-1, cbfa1) and ALP activity, compared to both BMP-2 treatment and laser irradiation alone. In vitro matrix mineralization was significantly accelerated by laser stimulation compared to that of the control, more so than with the combined laser/BMP-2 treatment. CONCLUSIONS:The present in vitro findings demonstrate that high-power, low-level Nd:YAG laser increased osteoblast activity, very efficiently accelerating mineral deposition. Osteoinductive effect of laser is likely mediated by activation of BMP-2-related signaling pathway.
journal_name
Lasers Surg Medjournal_title
Lasers in surgery and medicineauthors
Kim IS,Cho TH,Kim K,Weber FE,Hwang SJdoi
10.1002/lsm.20870subject
Has Abstractpub_date
2010-08-01 00:00:00pages
510-8issue
6eissn
0196-8092issn
1096-9101journal_volume
42pub_type
杂志文章abstract:BACKGROUND AND OBJECTIVE:Infrared neural stimulation (INS) has recently evoked interest as an alternative to electrical stimulation. The mechanism of activation is the heating of water, which induces changes in cell membrane potential but may also trigger heat sensitive receptors. To further elucidate the mechanism, wh...
journal_title:Lasers in surgery and medicine
pub_type: 杂志文章
doi:10.1002/lsm.22158
更新日期:2013-09-01 00:00:00
abstract::This study characterizes the special properties of hematoporphyrin derivative (HpD) (Photofrin I) and Photofrin II with respect to the absorption and fluorescence spectra of the subfractions of the compounds in both solution and cells. In addition, data are presented demonstrating variation in the spectral amplitudes ...
journal_title:Lasers in surgery and medicine
pub_type: 杂志文章
doi:10.1002/lsm.1900070207
更新日期:1987-01-01 00:00:00
abstract::The effects of picosecond laser-induced optical breakdown on tissue were investigated using high-intensity 40 ps Nd:YAG laser pulses at 1.06 microns. Tissue damage was evaluated using the corneal endothelium in vitro as a model system. Systematic studies were performed to determine the scaling of the tissue damage and...
journal_title:Lasers in surgery and medicine
pub_type: 杂志文章,评审
doi:10.1002/lsm.1900090302
更新日期:1989-01-01 00:00:00
abstract:OBJECTIVE:Inflammation is a well-known consequence of surgery. Although surgical debulking of tumor is beneficial to patients, the onset of inflammation in injured tissue may impede the success of adjuvant therapies. One marker for postoperative inflammation is IL-6, which is released as a consequence of surgical injur...
journal_title:Lasers in surgery and medicine
pub_type: 杂志文章,随机对照试验
doi:10.1002/lsm.22934
更新日期:2018-07-01 00:00:00
abstract:BACKGROUND AND OBJECTIVES:Efficacy of topical anesthetics can be enhanced by pretreatment of the skin with ablative fractional lasers. However, little is known about the role of parameters such as laser modality and laser density settings in this technique. Aims of this study were to compare the efficacy of pretreatmen...
journal_title:Lasers in surgery and medicine
pub_type: 杂志文章,随机对照试验
doi:10.1002/lsm.22936
更新日期:2018-10-01 00:00:00
abstract:BACKGROUND AND OBJECTIVES:Thermal lasers are routinely used to treat certain retinal disorders although they cause collateral damage to photoreceptors. The current study evaluated a confined, non-conductive thermal, 3-nanosecond pulse laser in order to determine how to produce the greatest therapeutic range without cau...
journal_title:Lasers in surgery and medicine
pub_type: 杂志文章
doi:10.1002/lsm.21087
更新日期:2011-08-01 00:00:00
abstract::A technique for partial nephrectomy using a combination of an Ultrasonic Surgical Aspirator and a neodymium:YAG laser provided better hemostasis and decreased total operative time compared to standard methods. Delayed hemorrhage was not seen, and thermal damage to the remaining renal parenchyma was minimal. ...
journal_title:Lasers in surgery and medicine
pub_type: 杂志文章
doi:10.1002/lsm.1900060210
更新日期:1986-01-01 00:00:00
abstract:BACKGROUND AND OBJECTIVES:Whether light emitting diode (LED) irradiation has effects on "beta-amyloid (A beta) induced apoptosis," a leading hypothesis of the cause of Alzheimer's disease (AD), or not? STUDY DESIGN/MATERIALS AND METHODS:Monolayer cell cultures of PC12 were subjected to A beta or/and LED irradiation at...
journal_title:Lasers in surgery and medicine
pub_type: 杂志文章
doi:10.1002/lsm.10216
更新日期:2003-01-01 00:00:00
abstract::The temperature developed by the laser thermal ("hot tip") probe during arterial recanalisation is primarily dependent on the rate of energy delivery and the rate of dissipation to the surrounding medium. While higher probe tip temperatures enhance the efficacy of atheroma ablation, so too is the incidence of adverse ...
journal_title:Lasers in surgery and medicine
pub_type: 杂志文章
doi:10.1002/lsm.1900100204
更新日期:1990-01-01 00:00:00
abstract:BACKGROUND AND OBJECTIVES:While cryolipolysis initially received FDA clearance for fat reduction in the abdomen and flanks, there was significant interest in non-surgical fat reduction for other sites, such as the inner and outer thighs. This article reports the results of an inner thigh study which contributed to FDA ...
journal_title:Lasers in surgery and medicine
pub_type: 临床试验,杂志文章,多中心研究
doi:10.1002/lsm.22320
更新日期:2015-02-01 00:00:00
abstract:BACKGROUND AND OBJECTIVE:To evaluate safety and efficacy of treatment with the picosecond Nd:YAG 532 nm for lentigines in Asian skin. STUDY DESIGN/MATERIALS AND METHODS:This was a prospective, open-label cohort study, using a novel picosecond 532-nm laser for the treatment of facial lentigines. Subjects received up to...
journal_title:Lasers in surgery and medicine
pub_type: 杂志文章
doi:10.1002/lsm.23103
更新日期:2019-11-01 00:00:00
abstract:BACKGROUND AND OBJECTIVES:The sanitation of the root canal system and the adjacent dentin has always been a key requirement for successful endodontics. In recent years, various laser systems have provided a major contribution to this aim, namely the Nd:YAG-, the 810 nm Diode-, the Er:YAG-, and the Er,Cr:YSGG laser. Num...
journal_title:Lasers in surgery and medicine
pub_type: 杂志文章
doi:10.1002/lsm.20331
更新日期:2006-07-01 00:00:00
abstract:BACKGROUND AND OBJECTIVE:The clinical use of lasers to cut and coagulate tissue necessitates a better understanding of how the residual thermal damage affects healing. This study was designed to evaluate the effects of varying degrees of thermal damage on the healing process. STUDY DESIGN, MATERIALS AND METHODS:Partia...
journal_title:Lasers in surgery and medicine
pub_type: 杂志文章
doi:10.1002/(SICI)1096-9101(1996)18:1<1::AID-LSM1>3.0.
更新日期:1996-01-01 00:00:00
abstract:BACKGROUND AND OBJECTIVE:The reactive oxygen mechanisms associated with cell damage after photodynamic therapy (PDT) may be exploited to enhance tumor destruction. Pharmacological reduction of glutathione (GSH), an inhibitor of reactive oxygen species, can be induced by administration of buthionine sulfoximine (BSO). ...
journal_title:Lasers in surgery and medicine
pub_type: 杂志文章
doi:10.1002/(sici)1096-9101(1998)23:3<161::aid-lsm5>3.
更新日期:1998-01-01 00:00:00
abstract:BACKGROUND AND OBJECTIVES:Non-invasive laser skin treatment modalities are generally designed to protect the epidermis by cooling and limiting the laser energy deposition in accordance with skin type. We explore a treatment modality that uses a 2,940 nm Er:YAG laser with high tissue absorption to ablate an array of cha...
journal_title:Lasers in surgery and medicine
pub_type: 杂志文章,评审
doi:10.1002/lsm.22533
更新日期:2017-01-01 00:00:00
abstract:BACKGROUND AND OBJECTIVE:Light dosimetry for endobronchial photodynamic therapy is not very advanced to date. This study investigates the dependency of the fluence rate distribution in the bronchial wall on several parameters. STUDY DESIGN/MATERIALS AND METHODS:A Monte Carlo model is employed for the illumination of a...
journal_title:Lasers in surgery and medicine
pub_type: 杂志文章
doi:10.1002/(sici)1096-9101(1998)22:4<193::aid-lsm3>3.
更新日期:1998-01-01 00:00:00
abstract::A synthetic contact sapphire endoprobe attached to the quartz fiber of a neodymium:yttrium aluminum garnet (Nd:YAG) laser had been used in the transurethral treatment of bladder tumors. A total of 68 operations have been carried out in 48 patients. The contact probe was placed directly into the tumor under direct visi...
journal_title:Lasers in surgery and medicine
pub_type: 杂志文章
doi:10.1002/lsm.1900070616
更新日期:1987-01-01 00:00:00
abstract:BACKGROUND:Acne scarring is a common disfiguring sequela of acne vulgaris which can lead to serious psychosocial problems and have a negative effect on patients' quality of life. Although a variety of approaches can be used to treat atrophic acne scars, disadvantages such as long-healing time, dyspigmentation, infectio...
journal_title:Lasers in surgery and medicine
pub_type: 临床试验,杂志文章
doi:10.1002/lsm.22825
更新日期:2018-10-01 00:00:00
abstract:BACKGROUND AND OBJECTIVES:To investigate the effect of photodynamic therapy (PDT) on cell death in malignant tumor tissue, the frequency of terminal deoxynucleotidyl transferase-mediated dUTP nick end-labeling (TUNEL)-positive cells and the possible involvement of Fas and Fas ligand system were evaluated. STUDY DESIGN...
journal_title:Lasers in surgery and medicine
pub_type: 杂志文章
doi:10.1002/1096-9101(2000)26:5<449::aid-lsm4>3.0.co;2
更新日期:2000-01-01 00:00:00
abstract:BACKGROUND AND OBJECTIVES:Optical microangiography (OMAG) is a noninvasive technique capable of imaging 3D microvasculature. OMAG-based optical lymphangiography has been developed for 3D visualization of lymphatic vessels without the need for exogenous contrast agents. In this study, we utilize the optical lymphangiogr...
journal_title:Lasers in surgery and medicine
pub_type: 杂志文章
doi:10.1002/lsm.22387
更新日期:2015-10-01 00:00:00
abstract:OBJECTIVE:Acute radiodermatitis (RD) is a distressing and painful skin reaction that occurs in 95% of the patients undergoing radiotherapy (RT). The aim of this study was to evaluate the effectiveness of photobiomodulation therapy (PBMT) in the prevention of acute RD in breast cancer (BC) patients undergoing RT. METHO...
journal_title:Lasers in surgery and medicine
pub_type: 杂志文章
doi:10.1002/lsm.22804
更新日期:2018-02-10 00:00:00
abstract:BACKGROUND AND OBJECTIVES:This study was conducted to clarify the morphologic characteristics and subsequent repair process of coagulation necrosis produced by pulsed CO(2) laser irradiation with relatively low fluence, and thereby to evaluate the clinical efficacy of this irradiation mode. STUDY DESIGN/MATERIALS AND ...
journal_title:Lasers in surgery and medicine
pub_type: 杂志文章
doi:10.1002/lsm.20712
更新日期:2008-12-01 00:00:00
abstract:BACKGROUND AND OBJECTIVE:Recent studies have sought to utilize diode laser "micropulsing" in order to preserve therapeutic efficacy of retinal photocoagulation while minimizing pain and subjacent tissue injury. A model for the transient thermal tissue response to continuous and micropulsed diode laser output is present...
journal_title:Lasers in surgery and medicine
pub_type: 杂志文章
doi:10.1002/(sici)1096-9101(1997)20:4<409::aid-lsm6>3.
更新日期:1997-01-01 00:00:00
abstract:BACKGROUND AND OBJECTIVE:Most women with early stage breast cancer have the option of breast conserving therapy, which involves a partial mastectomy for removal of the primary tumor, usually followed by radiotherapy. The presence of tumor at or near the margin is strongly correlated with the risk of local tumor recurre...
journal_title:Lasers in surgery and medicine
pub_type: 杂志文章
doi:10.1002/lsm.20865
更新日期:2010-01-01 00:00:00
abstract:BACKGROUND AND OBJECTIVES:Holmium laser lithotripsy is the gold standard for intracorporeal fragmentation of urinary calculi. Usually, a visible beam is superimposed on the IR treatment laser as an aiming beam to guide the surgeon. In vitro tests showed that this aiming beam (532 nm, power <1 mW) excites strong fluores...
journal_title:Lasers in surgery and medicine
pub_type: 杂志文章
doi:10.1002/lsm.22611
更新日期:2017-04-01 00:00:00
abstract:BACKGROUND AND OBJECTIVE:Cutaneous and mucocutaneous leishmaniasis are diseases characterized by skin or mucosal manifestations. In the new world, Leishmania braziliensis is the main etiological agent of cutaneous leishmaniasis, condition that may evolve to the mucocutaneous form. The therapeutic arsenal routinely empl...
journal_title:Lasers in surgery and medicine
pub_type: 杂志文章
doi:10.1002/lsm.22099
更新日期:2012-12-01 00:00:00
abstract:BACKGROUND AND OBJECTIVES:Terawatt (TW) lasers have become commonplace since the development of the chirped-pulse amplification method using Ti:sapphire and Nd:glass laser rods. We have measured the minimum visible lesion (MVL) thresholds for porcine1The animals involved in this study were procured, maintained, and use...
journal_title:Lasers in surgery and medicine
pub_type: 杂志文章
doi:10.1002/lsm.20195
更新日期:2005-07-01 00:00:00
abstract::Since most techniques of refractive surgery currently in use or being developed have the potential for significant side effects, there is a need for investigating alternative procedures. We herein report on the use of a pulsed CO2 laser beam delivered through a pair of complementary axicons to produce a ring of stroma...
journal_title:Lasers in surgery and medicine
pub_type: 杂志文章
doi:10.1002/lsm.1900120305
更新日期:1992-01-01 00:00:00
abstract::The author's experience in the use of the argon laser in the treatment of superficial vascular lesions in children is reviewed. Deteriorating portwine stains, strawberry hemangiomas, other hemangiomas, telangiectasias, and other lesions are discussed. Their natural histories are discussed. Details are given regarding ...
journal_title:Lasers in surgery and medicine
pub_type: 杂志文章
doi:10.1002/lsm.1900060106
更新日期:1986-01-01 00:00:00
abstract:OBJECTIVES:Laser lithotripsy, often used during ureteronephroscopy (URNS), requires the Ho:YAG optical fiber transmit energy via total internal reflection (TIR). In critical lower pole deflections, energy may refract into the cladding causing fiber failure and scope damage. New optical fiber technology aims to have inc...
journal_title:Lasers in surgery and medicine
pub_type: 杂志文章
doi:10.1002/lsm.22822
更新日期:2018-10-01 00:00:00