Abstract:
BACKGROUND AND OBJECTIVE:Cutaneous and mucocutaneous leishmaniasis are diseases characterized by skin or mucosal manifestations. In the new world, Leishmania braziliensis is the main etiological agent of cutaneous leishmaniasis, condition that may evolve to the mucocutaneous form. The therapeutic arsenal routinely employed to treat infected patients is unsatisfactory, especially for pentavalent antimonials, treatment recommended by the WHO, as they are often highly toxic, poorly tolerated and of variable effectiveness. This work aimed to evaluate in vitro the effectiveness of photodynamic antimicrobial chemotherapy as a new approach for the treatment of leishmaniasis. MATERIALS AND METHODS:A laser (λ = 660 nm, 40 mW, 4.2 J/cm2 , and 8.4 J/cm2 , CW) associated to phenothiazine's derivatives (5 and 10 µg/ml, toluidine blue O, methylene blue, or phenothiazine) on the promastigote forms of L. braziliensis in a single session. Samples were removed and analyzed in a hemocytometer 72 hours after PACT and viability of the parasites was assessed in quadruplicates. RESULTS:An important decrease in the number of viable parasites on all treated groups in comparison to their controls was observed as all tested compounds lead to significant parasite lethality being the highest lethality achieved with 10 µg/ml of TBO. No lethality was observed on groups treated with laser or with any of the compounds separately. CONCLUSIONS:TBO presented higher parasite lethality in comparison to MB with impressive reduction from 1 hour to 5 minutes of pre-incubation time.
journal_name
Lasers Surg Medjournal_title
Lasers in surgery and medicineauthors
Barbosa AF,Sangiorgi BB,Galdino SL,Barral-Netto M,Pitta IR,Pinheiro ALdoi
10.1002/lsm.22099subject
Has Abstractpub_date
2012-12-01 00:00:00pages
850-5issue
10eissn
0196-8092issn
1096-9101journal_volume
44pub_type
杂志文章abstract:BACKGROUND AND OBJECTIVE:Concerning current clinical practice, laser-assisted lipoplasty is still secondary to other procedures. In order to evaluate effects of thermal interaction with fatty-tissue, a near infrared diode laser was examined under reproducible conditions. METHODS:Based on optical spectroscopy of fatty-...
journal_title:Lasers in surgery and medicine
pub_type: 杂志文章
doi:10.1002/lsm.20602
更新日期:2008-02-01 00:00:00
abstract:BACKGROUND AND OBJECTIVE:A semi-solid albumin solder formulated with hydroxypropylmethylcellulose (HPMC) was designed to improve the characteristics of liquid and solid solders. STUDY DESIGN/MATERIALS AND METHODS:Acute tensile strengths were determined on canine small bowel in vitro by using liquid 50% bovine serum al...
journal_title:Lasers in surgery and medicine
pub_type: 杂志文章
doi:10.1002/1096-9101(2000)27:2<140::aid-lsm5>3.0.co;2
更新日期:2000-01-01 00:00:00
abstract::Hyperthermia induced by a microwave source (2,450 MHz) was used alone and in combination with photodynamic therapy (PDT) to treat the SMT-F, EMT-6, and RIF animal tumors in vivo. PDT was administered using either Photofrin I or II as the photosensitizer and an argon-pumped tunable dye laser (630 nm) as the light sourc...
journal_title:Lasers in surgery and medicine
pub_type: 杂志文章
doi:10.1002/lsm.1900070104
更新日期:1987-01-01 00:00:00
abstract:BACKGROUND AND OBJECTIVES:Hyperhidrosis is a condition that affects a large percentage of the population and has a significant impact on peoples' lives. This report presents a technical overview of a new noninvasive, microwave-based device for creating thermolysis of sweat glands. The fundamental principles of operatio...
journal_title:Lasers in surgery and medicine
pub_type: 杂志文章
doi:10.1002/lsm.21142
更新日期:2012-01-01 00:00:00
abstract::The temperature developed by the laser thermal ("hot tip") probe during arterial recanalisation is primarily dependent on the rate of energy delivery and the rate of dissipation to the surrounding medium. While higher probe tip temperatures enhance the efficacy of atheroma ablation, so too is the incidence of adverse ...
journal_title:Lasers in surgery and medicine
pub_type: 杂志文章
doi:10.1002/lsm.1900100204
更新日期:1990-01-01 00:00:00
abstract:BACKGROUND AND OBJECTIVE:The main factors in photodynamic therapy (PDT) are: photosensitizer retention, photon absorption, and oxygen supply. Each factor has its unique set of problems that poses limitation to the treatment. Both light delivery and oxygen supply are significant bottlenecks in PDT. Vascular closure duri...
journal_title:Lasers in surgery and medicine
pub_type: 杂志文章
doi:10.1002/lsm.20951
更新日期:2010-09-01 00:00:00
abstract:BACKGROUND:Lymphangioma circumscriptum (LC) is a rare, benign, vascular malformation of dilated superficial lymphatic channels. Treatment is typically undertaken for cosmetic reasons or complications such as fluid drainage, pain, and infection risk that can negatively impact quality of life. Management of LC remains ch...
journal_title:Lasers in surgery and medicine
pub_type: 杂志文章
doi:10.1002/lsm.22379
更新日期:2015-09-01 00:00:00
abstract:BACKGROUND AND OBJECTIVES:The risks of currently available invasive procedures in body contouring motivate a need for safer, non-invasive technologies for improving the appearance of body silhouette. A new device has been developed that uses focused therapeutic ultrasound to reduce adipose tissue non-invasively. The ai...
journal_title:Lasers in surgery and medicine
pub_type: 临床试验,杂志文章
doi:10.1002/lsm.20478
更新日期:2007-04-01 00:00:00
abstract:BACKGROUND AND OBJECTIVES:Terawatt (TW) lasers have become commonplace since the development of the chirped-pulse amplification method using Ti:sapphire and Nd:glass laser rods. We have measured the minimum visible lesion (MVL) thresholds for porcine1The animals involved in this study were procured, maintained, and use...
journal_title:Lasers in surgery and medicine
pub_type: 杂志文章
doi:10.1002/lsm.20195
更新日期:2005-07-01 00:00:00
abstract:BACKGROUND AND OBJECTIVE:Dynamics of cryogen spray deposition, water condensation and frost formation is studied in relationship to cooling rate and efficiency of cryogen spray cooling (CSC) in combination with laser dermatologic surgery. STUDY DESIGN/MATERIALS AND METHODS:A high-speed video camera was used to image t...
journal_title:Lasers in surgery and medicine
pub_type: 杂志文章
doi:10.1002/lsm.1076
更新日期:2001-01-01 00:00:00
abstract:BACKGROUND AND OBJECTIVE:Despite laser applications targetted toward tonsillar tissue, there has been no characterization of underlying optical and thermal events during laser irradiation of tonsillar tissue. STUDY DESIGN/MATERIALS AND METHODS:The optical properties of canine and human tonsils were determined at 805 n...
journal_title:Lasers in surgery and medicine
pub_type: 杂志文章
doi:10.1002/lsm.1055
更新日期:2001-01-01 00:00:00
abstract::We hypothesized that autologous clot deposited on the luminal surface of laser vascular welds immediately after creation would produce higher time zero bursting pressures that could be achieved in welds perfused with saline alone. To test this hypothesis, we compared bursting pressures of welds created in isolated rab...
journal_title:Lasers in surgery and medicine
pub_type: 杂志文章
doi:10.1002/lsm.1900100414
更新日期:1990-01-01 00:00:00
abstract:BACKGROUND AND OBJECTIVES:In Holmium laser lithotripsy, usually, the surgeon is guided by a visible beam superimposing the infrared (IR) treatment radiation. It has been shown that a green aiming beam excites stone autofluorescence. This fluorescence signal can be used for calculi detection to check the correct fiber p...
journal_title:Lasers in surgery and medicine
pub_type: 杂志文章
doi:10.1002/lsm.23154
更新日期:2020-06-01 00:00:00
abstract::The CO2 laser has provided the otolaryngologist/head and neck surgeon with a valuable addition in the diagnosis and treatment of malignant disease of the oral cavity. It is also helpful in the treatment of the premalignant clinical leukoplakias of the oral cavity. Carcinoma in situ has been diagnosed and treated succe...
journal_title:Lasers in surgery and medicine
pub_type: 杂志文章
doi:10.1002/lsm.1900060504
更新日期:1986-01-01 00:00:00
abstract:BACKGROUND AND OBJECTIVES:This study was conducted to clarify the morphologic characteristics and subsequent repair process of coagulation necrosis produced by pulsed CO(2) laser irradiation with relatively low fluence, and thereby to evaluate the clinical efficacy of this irradiation mode. STUDY DESIGN/MATERIALS AND ...
journal_title:Lasers in surgery and medicine
pub_type: 杂志文章
doi:10.1002/lsm.20712
更新日期:2008-12-01 00:00:00
abstract:OBJECTIVES:This review has the following objectives: Firstly, it provides an explanation of the evolution of laser/intense pulsed light (IPL) hair reduction modalities from high fluence professional devices to low fluence home-use appliances. Secondly, it summarises published literature reviews on home-use devices (HUD...
journal_title:Lasers in surgery and medicine
pub_type: 杂志文章,评审
doi:10.1002/lsm.23061
更新日期:2019-08-01 00:00:00
abstract::Methods used to revascularize ischemic myocardium have included arterial reconstruction, coronary artery bypass grafting, providing direct circulation from the intraventricular chamber, and techniques to promote collateral circulation. Ventricular channels from the epicardial surface through the endocardium are readil...
journal_title:Lasers in surgery and medicine
pub_type: 杂志文章
doi:10.1002/lsm.1900030307
更新日期:1983-01-01 00:00:00
abstract:BACKGROUND AND OBJECTIVE:Soft tissue sarcomas (STS) are a rare and heterogeneous group of malignant tumors that are often treated through surgical resection. Current intraoperative margin assessment methods are limited and highlight the need for an improved approach with respect to time and specificity. Here we investi...
journal_title:Lasers in surgery and medicine
pub_type: 临床试验,杂志文章
doi:10.1002/lsm.22564
更新日期:2016-10-01 00:00:00
abstract:BACKGROUND AND OBJECTIVES:Porcine skin is a widely used model in diffusion studies, but its usefulness for laser-assisted drug delivery (LADD) has not been evaluated in comparison with human skin. This study compared porcine and human skin in ex vivo LADD diffusion studies. STUDY DESIGN/MATERIALS AND METHODS:Ex vivo a...
journal_title:Lasers in surgery and medicine
pub_type:
doi:10.1002/lsm.23344
更新日期:2020-11-08 00:00:00
abstract:BACKGROUND AND OBJECTIVES:To evaluate the antiallergic effect of low-level laser irradiation (LLLI) at 650 nm in a mouse model of allergic rhinitis (AR), and to examine the underlying mechanisms. STUDY DESIGN/MATERIALS AND METHODS:BALB/c mice were sensitized with ovalbumin (OVA) and alum and challenged intranasally wi...
journal_title:Lasers in surgery and medicine
pub_type: 杂志文章
doi:10.1002/lsm.23141
更新日期:2020-04-01 00:00:00
abstract::Current laser dentistry since our initial studies in 1964 is reviewed. Three areas are considered: routine dental office practice, tooth restoration, and oral surgery. For tooth restoration and oral surgery, CO2 and neodymium:YAG lasers are used and lasers are used in photodynamic therapy for oral cancer. Dental offic...
journal_title:Lasers in surgery and medicine
pub_type: 杂志文章
doi:10.1002/lsm.1900060616
更新日期:1987-01-01 00:00:00
abstract::Assessments of non-surgical office-based aesthetic treatments' efficacy including lasers are mostly based on subjective evaluation of clinical photography. Accumulating evidence suggest that non-invasive skin imaging such as optical coherence tomography (OCT) can be effectively used to assess not only the skin surface...
journal_title:Lasers in surgery and medicine
pub_type: 杂志文章,评审
doi:10.1002/lsm.23219
更新日期:2020-10-01 00:00:00
abstract:BACKGROUND AND OBJECTIVES:Helium plasma skin regeneration (PSR) is a novel skin rejuvenation technology with significant differences compared with nitrogen PSR technology but that may exert similar skin tissue effects. Study objectives included a comparison of acute and chronic skin tissue changes among the two plasmas...
journal_title:Lasers in surgery and medicine
pub_type:
doi:10.1002/lsm.23167
更新日期:2020-01-01 00:00:00
abstract:BACKGROUND AND OBJECTIVE:Wound-healing delays caused by lateral thermal damage to tissue remain a drawback of CO(2) surgical lasers. This study compares the thermal damage and wound-healing properties of a 7.5-micros pulsed CO(2) laser with scalpel and continuous wave (CW) CO(2) laser incisions. STUDY DESIGN/MATERIALS...
journal_title:Lasers in surgery and medicine
pub_type: 杂志文章
doi:10.1002/(sici)1096-9101(2000)26:1<22::aid-lsm5>3.0
更新日期:2000-01-01 00:00:00
abstract:BACKGROUND AND OBJECTIVE:Interstitial photodynamic therapy (iPDT) of non-resectable recurrent glioblastoma using 5-aminolevulinic acid (ALA)-induced protoporphyrin IX (PpIX) has shown a promising outcome. It remained unclear, however, to what extent inter- and intra-tumoural differences of PpIX concentrations influence...
journal_title:Lasers in surgery and medicine
pub_type: 临床试验,杂志文章
doi:10.1002/lsm.22126
更新日期:2013-04-01 00:00:00
abstract::A low-energy Nd:YAG laser was used to irradiate extracted human teeth coated with a black energy-absorbent laser initiator in a study to determine the extent of the morphologic changes produced in the enamel surface. The laser initiator was applied to a cleaned enamel surface and irradiated at an energy output of 30 m...
journal_title:Lasers in surgery and medicine
pub_type: 杂志文章
doi:10.1002/lsm.1900100510
更新日期:1990-01-01 00:00:00
abstract:BACKGROUND:Platelet-rich plasma (PRP) which contains large amounts of growth factors has been tried to enhance therapeutic efficacy of laser treatment for acne scar with unknown underlying mechanism. OBJECTIVES:The present study was conducted to investigate the molecular mechanism of increased clinical efficacy of PRP...
journal_title:Lasers in surgery and medicine
pub_type: 杂志文章,随机对照试验
doi:10.1002/lsm.22776
更新日期:2018-04-01 00:00:00
abstract:OBJECTIVE:The objective of this study was to evaluate the effectiveness of the pulsed dye laser (585 nm, 450 ms) in the treatment of sun induced wrinkles. DESIGN:Patients had one pulsed dye laser (585 nm) treatment. The treated areas were assessed by the following methods: grading of skin wrinkles at 6 weeks, 12 weeks...
journal_title:Lasers in surgery and medicine
pub_type: 杂志文章
doi:10.1002/(sici)1096-9101(1999)25:3<229::aid-lsm7>3.
更新日期:1999-01-01 00:00:00
abstract:BACKGROUND AND OBJECTIVE:The clinical use of lasers to cut and coagulate tissue necessitates a better understanding of how the residual thermal damage affects healing. This study was designed to evaluate the effects of varying degrees of thermal damage on the healing process. STUDY DESIGN, MATERIALS AND METHODS:Partia...
journal_title:Lasers in surgery and medicine
pub_type: 杂志文章
doi:10.1002/(SICI)1096-9101(1996)18:1<1::AID-LSM1>3.0.
更新日期:1996-01-01 00:00:00
abstract:BACKGROUND AND OBJECTIVE:Low-intensity laser irradiation (LILI) has been used to modulate a variety of biological processes, including diabetic wound healing. The mechanism of action is thought to exist primarily with the mitochondria. This study aimed to determine the effect of irradiation on normal, diabetic, and isc...
journal_title:Lasers in surgery and medicine
pub_type: 杂志文章
doi:10.1002/lsm.22027
更新日期:2012-07-01 00:00:00