Wound healing and collagen thermal damage in 7.5-microsec pulsed CO(2) laser skin incisions.

Abstract:

BACKGROUND AND OBJECTIVE:Wound-healing delays caused by lateral thermal damage to tissue remain a drawback of CO(2) surgical lasers. This study compares the thermal damage and wound-healing properties of a 7.5-micros pulsed CO(2) laser with scalpel and continuous wave (CW) CO(2) laser incisions. STUDY DESIGN/MATERIALS AND METHODS:We created incisions on the dorsal pelts of rats with a 7.5-micros pulsed CO(2) laser at 5-, 10-, or 15-Hz repetition rate, a conventional CW laser, or scalpel. Animals were euthanized at postoperative days 3, 7, 14, 21, and 80. Tissue was harvested and analyzed histologically and for wound tensile strength. In addition, tissue was harvested acutely and analyzed for acute thermal injury lateral to the incisions. RESULTS:Incisions made with the pulsed laser had significantly higher tensile strength and histologic rankings than did CW laser incisions at days 3-21, producing 118 microm of thermal damage to tissue as compared with 333 microm for CW laser. Pulsed laser incisions were not statistically different than scalpel incisions at days 3-14 of healing. Mathematical modeling showed the pulsed laser to produce a wound healing delay of 1.0 day by tensiometry and 1.9 days by histology, compared with 3.2 days by tensiometry and 6.0 days by histology for CW laser. There were no significant differences in wound healing when the pulsed laser was used at repetition rates of 5-15 Hz. CONCLUSIONS:Using a 7.5-micros pulse duration, CO(2) laser incisions healed at a rate similar to scalpel incisions and reduced the wound-healing delay seen with typical surgical CO(2) lasers.

journal_name

Lasers Surg Med

authors

Sanders DL,Reinisch L

doi

10.1002/(sici)1096-9101(2000)26:1<22::aid-lsm5>3.0

subject

Has Abstract

pub_date

2000-01-01 00:00:00

pages

22-32

issue

1

eissn

0196-8092

issn

1096-9101

pii

10.1002/(SICI)1096-9101(2000)26:1<22::AID-LSM5>3.0

journal_volume

26

pub_type

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