Phototoxic effect of photodynamic therapy on lung cancer cells grown as a monolayer and three dimensional multicellular spheroids.

Abstract:

BACKGROUND AND OBJECTIVE:Photodynamic therapy (PDT) is a minimally invasive therapeutic modality for the treatment of neoplastic and non-neoplastic diseases. The photochemical interaction of light, photosensitizer (PS) and molecular oxygen produces singlet oxygen which induces cell death. The effectiveness of zinc sulfophthalocyanine (ZnPcSmix ) has been shown on A549 monolayers and not on MCTS. The objective of this study was to investigate whether the same pattern of cell death would be induced in lung cancer cells (A549) grown as monolayer cells compared to three dimensional multicellular tumor spheroids (MCTS) using the same laser parameters. MATERIALS AND METHODS:Commercially purchased A549 cells used in this study were cultured as monolayers and as MCTS. ZnPcSmix at different concentrations [0, 5, 10, 20, and 40 µM] was used and activated at a wavelength of 680 nm with 5 J/cm2. Lysosomal and mitochondrial damage after PDT and reactive oxygen species (ROS) production were determined by fluorescent microscopy. Changes in cellular responses were evaluated using cell morphology, viability, proliferation, cytotoxicity, and cell death analysis. RESULTS:Cells exposed to neither laser light nor PS, showed no changes in cell morphology, proliferation, cytotoxicity, and ROS production. However, cells treated with light activated ZnPcSmix resulted in a significant production of ROS and a dose dependant decrease in viability and proliferation as well as an increase in cell membrane damage in both monolayer and MCTSs. CONCLUSION:ZnPcSmix PS used in this study induced damage to vital organelles and was effective in inducing apoptosis in lung cancer cells grown as a monolayer and MTCS through ROS production.

journal_name

Lasers Surg Med

authors

Manoto SL,Houreld NN,Abrahamse H

doi

10.1002/lsm.22121

subject

Has Abstract

pub_date

2013-03-01 00:00:00

pages

186-94

issue

3

eissn

0196-8092

issn

1096-9101

journal_volume

45

pub_type

杂志文章
  • Laser soldering of rat skin, using fiberoptic temperature controlled system.

    abstract:BACKGROUND AND OBJECTIVE:Laser soldering of tissues is based on the application of a biological solder on the approximated edges of a cut. Our goal was to use laser soldering for sealing cuts in skin under temperature feedback control and compare the results with ones obtained using standard sutures. STUDY DESIGN/MATE...

    journal_title:Lasers in surgery and medicine

    pub_type: 杂志文章

    doi:10.1002/lsm.1118

    authors: Simhon D,Ravid A,Halpern M,Cilesiz I,Brosh T,Kariv N,Leviav A,Katzir A

    更新日期:2001-01-01 00:00:00

  • Massive air embolism complicating Nd-YAG laser endobronchial photoresection.

    abstract::A 63 year old male underwent 6,900 rads of external radiation for a squamous cell carcinoma of the left main bronchus. Recurrence of the tumor 8 months later was treated with 6,618 joules and patency of the left main bronchus was restored. Four months later, he developed complete atelectasis of the left lung requiring...

    journal_title:Lasers in surgery and medicine

    pub_type: 杂志文章

    doi:10.1002/lsm.1900120316

    authors: Golish JA,Pena CM,Mehta AC

    更新日期:1992-01-01 00:00:00

  • Current status of infrared fiber optics for medical laser power delivery.

    abstract::There are a host of fiber-optic delivery devices available for use with the Er:YAG, HF, CO, and CO2 lasers at 2.9, 2.8, 5.3, and 10.6 microns wavelengths, respectively. While many of these fibers are not as convenient to use as conventional silica fibers, they offer the ability to deliver infrared laser energy for a w...

    journal_title:Lasers in surgery and medicine

    pub_type: 杂志文章,评审

    doi:10.1002/lsm.1900130513

    authors: Merberg GN

    更新日期:1993-01-01 00:00:00

  • Control of fibroblast populated collagen lattice contraction by antibody targeted photolysis of fibroblasts.

    abstract:BACKGROUND AND OBJECTIVE:Hypertrophic scarring and rigid scar contracture are disorders of wound healing for which there is presently no effective therapy. The dermal fibroblast plays a major role in scar fibrillogenesis and contracture. The objective of this study was to establish a selective and effective method to d...

    journal_title:Lasers in surgery and medicine

    pub_type: 杂志文章

    doi:10.1002/(sici)1096-9101(1997)21:3<235::aid-lsm3>3.

    authors: Strong LH,Berthiaume F,Yarmush ML

    更新日期:1997-01-01 00:00:00

  • Optically mediated nerve stimulation: Identification of injury thresholds.

    abstract:BACKGROUND AND OBJECTIVE:Transient optical nerve stimulation is a promising new non-contact, spatially precise, artifact-free neural excitation technique useful in research and clinical settings. This study evaluates safety of this pulsed infrared laser technique by histopathologic examination of stimulated peripheral ...

    journal_title:Lasers in surgery and medicine

    pub_type: 杂志文章

    doi:10.1002/lsm.20522

    authors: Wells JD,Thomsen S,Whitaker P,Jansen ED,Kao CC,Konrad PE,Mahadevan-Jansen A

    更新日期:2007-07-01 00:00:00

  • Facial teleangectasias: our experience in treatment with IPL.

    abstract:BACKGROUND AND OBJECTIVES:Facial teleangectasias can be a relevant cosmetic problem, which the patient usually hides with a thick layer of makeup. This study will describe the response on these vascular lesions using the intense pulsed light (IPL) source. METHODS:Five hundred eighteen consecutive patients were treated...

    journal_title:Lasers in surgery and medicine

    pub_type: 杂志文章

    doi:10.1002/lsm.20187

    authors: Clementoni MT,Gilardino P,Muti GF,Signorini M,Pistorale A,Morselli PG,Cavina C

    更新日期:2005-07-01 00:00:00

  • Laser threshold and cell damage mechanism for intravascular photoacoustic imaging.

    abstract:OBJECTIVES:Intravascular photoacoustic (IVPA) imaging is being developed to image atherosclerotic plaques, a leading cause of morbidity and mortality in the United States. However, the safety of this imaging modality, which requires repeated irradiation with short laser pulses, has not yet been investigated. This study...

    journal_title:Lasers in surgery and medicine

    pub_type: 杂志文章

    doi:10.1002/lsm.23026

    authors: Sowers T,VanderLaan D,Karpiouk A,Donnelly EM,Smith E,Emelianov S

    更新日期:2018-10-10 00:00:00

  • Combinatorial treatment of photothermal therapy using gold nanoshells with conventional photodynamic therapy to improve treatment efficacy: an in vitro study.

    abstract:BACKGROUND AND OBJECTIVE:Both photodynamic (PDT) and photothermal (PTT) therapy have proven to be effective treatment strategies for cancer, but the approach of combining them into a single treatment modality may offer better treatment efficacy. We compare the treatment efficacy of such combined treatment with the indi...

    journal_title:Lasers in surgery and medicine

    pub_type: 杂志文章

    doi:10.1002/lsm.20674

    authors: Kah JC,Wan RC,Wong KY,Mhaisalkar S,Sheppard CJ,Olivo M

    更新日期:2008-10-01 00:00:00

  • An intra-individual quantitative assessment of acute laser injury patterns in facial versus abdominal skin.

    abstract:INTRODUCTION:Clinical laser settings have traditionally been calibrated on abdominal skin to predict and anticipate patterns of injuries in facial skin. This experimental approach has limitations as facial skin and abdominal skin have differences that may influence the depth of laser injury. OBJECTIVE:The primary obje...

    journal_title:Lasers in surgery and medicine

    pub_type: 杂志文章

    doi:10.1002/lsm.21015

    authors: Bailey SH,Brown SA,Kim Y,Oni G,Abtahi F,Richardson JA,Hoopman J,Barton F,Kenkel JM

    更新日期:2011-02-01 00:00:00

  • Low level laser therapy for the management of breast cancer-related lymphedema: A randomized controlled feasibility study.

    abstract:OBJECTIVES:This study aimed to determine the feasibility of conducting a full scale randomized controlled trial investigating the effectiveness of low level laser therapy (LLLT), also known as photobiomodulation (PBM) therapy, used in addition to conventional therapy, for managing breast cancer related lymphedema (BCRL...

    journal_title:Lasers in surgery and medicine

    pub_type: 杂志文章,随机对照试验

    doi:10.1002/lsm.22947

    authors: Baxter GD,Liu L,Tumilty S,Petrich S,Chapple C,Anders JJ,Laser Lymphedema Trial Team.

    更新日期:2018-09-01 00:00:00

  • Extended theory of selective photothermolysis.

    abstract:BACKGROUND AND OBJECTIVE:We present a new theory of selective thermal damage of non-uniformly pigmented structures in biological tissues. Spatial separation of the heavily pigmented areas and the target requires limitation of the pigment temperature and heat diffusion from the pigmented to the targeted areas. STUDY DE...

    journal_title:Lasers in surgery and medicine

    pub_type: 杂志文章

    doi:10.1002/lsm.1136

    authors: Altshuler GB,Anderson RR,Manstein D,Zenzie HH,Smirnov MZ

    更新日期:2001-01-01 00:00:00

  • Photobiomodulation with polychromatic light increases zone 4 survival of transverse rectus abdominis musculocutaneous flap.

    abstract:OBJECTIVE:The aim of this study was to evaluate the effect of relatively novel approach of application of polychromatic light waves on flap survival of experimental musculocutaneous flap model and to investigate efficacy of this modality as a delay procedure to increase vascularization of zone 4 of transverse rectus ab...

    journal_title:Lasers in surgery and medicine

    pub_type: 杂志文章

    doi:10.1002/lsm.23063

    authors: Calis M,Demirtas TT,Sert G,Irmak G,Gumusderelioglu M,Turkkani A,Çakar AN,Ozgur F

    更新日期:2019-08-01 00:00:00

  • A multispectral fluorescence imaging system: design and initial clinical tests in intra-operative Photofrin-photodynamic therapy of brain tumors.

    abstract:BACKGROUND AND OBJECTIVES:Intra-operative identification of tumor by fluorescence may improve surgical resection or photodynamic therapy (PDT). A novel instrument was designed, constructed, and tested for this purpose. STUDY DESIGN/MATERIALS AND METHODS:The instrument was designed to provide high-resolution, multi-spe...

    journal_title:Lasers in surgery and medicine

    pub_type: 临床试验,杂志文章,随机对照试验

    doi:10.1002/lsm.10131

    authors: Yang VX,Muller PJ,Herman P,Wilson BC

    更新日期:2003-01-01 00:00:00

  • Evaluation of dentin root canal permeability after instrumentation and Er:YAG laser application.

    abstract:BACKGROUND AND OBJECTIVES:Smear layer removal with EDTA from root canal walls allows greater cleaning and disinfection of root canals. However, because Er:YAG laser acts on the removal of the smear layer, the objective of investigation was to analyze in vitro the effect of Er:YAG laser on dentin root canal wall permeab...

    journal_title:Lasers in surgery and medicine

    pub_type: 杂志文章

    doi:10.1002/(sici)1096-9101(2000)26:3<277::aid-lsm5>3.

    authors: Pecora JD,Brugnera-Júnior A,Cussioli AL,Zanin F,Silva R

    更新日期:2000-01-01 00:00:00

  • Influence of cavity preparation with Er:YAG Laser on enamel adjacent to restorations submitted to cariogenic challenge in situ: a polarized light microscopic analysis.

    abstract:BACKGROUND AND OBJECTIVES:Er:YAG laser has been used for caries removal and cavity preparation, using ablative parameters. Its effect on the margins of restorations submitted to cariogenic challenge has not yet been sufficiently investigated. The aim of this study was to assess the enamel adjacent to restored Er:YAG la...

    journal_title:Lasers in surgery and medicine

    pub_type: 杂志文章,随机对照试验

    doi:10.1002/lsm.20684

    authors: Chimello DT,Serra MC,Rodrigues AL Jr,Pécora JD,Corona SA

    更新日期:2008-11-01 00:00:00

  • Clinical benefit of using a multifractional Er:YAG laser combined with a spatially modulated ablative (SMA) module for the treatment of striae distensae: A prospective pilot study in 20 patients.

    abstract:BACKGROUND AND OBJECTIVE:Striae distensae (SD) are cutaneous lesions that often occur on the breasts, abdomen, hips, and thighs. The aim of this study is to evaluate the effectiveness of a new technique using a non-invasive Er:YAG laser combined with Spatially Modulated Ablation (SMA) module for the treatment of SD. S...

    journal_title:Lasers in surgery and medicine

    pub_type: 临床试验,杂志文章

    doi:10.1002/lsm.23042

    authors: Meningaud JP,SidAhmed-Mezi M,Billon R,Rem K,La Padula S,Hersant B

    更新日期:2019-03-01 00:00:00

  • A preclinical model to investigate the role of surgically-induced inflammation in tumor responses to intraoperative photodynamic therapy.

    abstract:OBJECTIVE:Inflammation is a well-known consequence of surgery. Although surgical debulking of tumor is beneficial to patients, the onset of inflammation in injured tissue may impede the success of adjuvant therapies. One marker for postoperative inflammation is IL-6, which is released as a consequence of surgical injur...

    journal_title:Lasers in surgery and medicine

    pub_type: 杂志文章,随机对照试验

    doi:10.1002/lsm.22934

    authors: Davis RW 4th,Papasavvas E,Klampatsa A,Putt M,Montaner LJ,Culligan MJ,McNulty S,Friedberg JS,Simone CB 2nd,Singhal S,Albelda SM,Cengel KA,Busch TM

    更新日期:2018-07-01 00:00:00

  • Determination of the dynamics of restored teeth by 3D electronic speckle pattern interferometry.

    abstract:BACKGROUND AND OBJECTIVES:The difficulties typically encountered in studying the effects of restorations on tooth reinforcement are often due to the 3-dimensional (3D) nature of deformation. Therefore, electronic 3D-Speckle-Interferometry (3D-ESPI) was used to assess the impact of different restorative materials on the...

    journal_title:Lasers in surgery and medicine

    pub_type: 杂志文章

    doi:10.1002/lsm.20036

    authors: Lang H,Rampado M,Müllejans R,Raab WH

    更新日期:2004-01-01 00:00:00

  • ED50 study of femtosecond terawatt laser pulses on porcine skin.

    abstract:BACKGROUND AND OBJECTIVES:Terawatt (TW) lasers have become commonplace since the development of the chirped-pulse amplification method using Ti:sapphire and Nd:glass laser rods. We have measured the minimum visible lesion (MVL) thresholds for porcine1The animals involved in this study were procured, maintained, and use...

    journal_title:Lasers in surgery and medicine

    pub_type: 杂志文章

    doi:10.1002/lsm.20195

    authors: Kumru SS,Cain CP,Noojin GD,Cooper MF,Imholte ML,Stolarski DJ,Cox DD,Crane CC,Rockwell BA

    更新日期:2005-07-01 00:00:00

  • Interstitial laser thermotherapy of a rat liver tumour: effect of hepatic inflow occlusion.

    abstract:BACKGROUND AND OBJECTIVE:Interstitial laser thermotherapy was used to treat rat liver tumours. The aim was to investigate the influence of temperature and temporary hepatic inflow occlusion on tumour growth and blood perfusion. STUDY DESIGN/MATERIALS AND METHODS:Liver tumours were treated at 44°C at the tumour border ...

    journal_title:Lasers in surgery and medicine

    pub_type: 杂志文章

    doi:10.1002/lsm.21024

    authors: Sturesson C,Ivarsson K,Stenram U,Andersson-Engels S,Tranberg KG

    更新日期:2011-01-01 00:00:00

  • Effects of Er:YAG and Nd:YAG laser irradiation on radicular dentine permeability using different irrigating solutions.

    abstract:BACKGROUND AND OBJECTIVES:To evaluate the effect of Er:YAG and Nd:YAG laser on radicular dentine permeability when using distilled and deionized water and 1% NaClO as irrigating solutions. STUDY DESIGN/MATERIALS AND METHODS:Thirty human maxillary canines were divided randomly into six groups. The root canals were inst...

    journal_title:Lasers in surgery and medicine

    pub_type: 杂志文章

    doi:10.1002/lsm.10214

    authors: Brugnera A Jr,Zanin F,Barbin EL,Spanó JC,Santana R,Pécora JD

    更新日期:2003-01-01 00:00:00

  • Feasibility of a Video-Mosaicking Approach to Extend the Field-of-View For Reflectance Confocal Microscopy in the Oral Cavity In Vivo.

    abstract:BACKGROUND:Reflectance confocal microscopy (RCM) is a developing approach for noninvasive detection of oral lesions with label-free contrast and cellular-level resolution. For access into the oral cavity, confocal microscopes are being configured with small-diameter telescopic probes and small objective lenses. However...

    journal_title:Lasers in surgery and medicine

    pub_type: 杂志文章

    doi:10.1002/lsm.23090

    authors: Peterson G,Zanoni DK,Ardigo M,Migliacci JC,Patel SG,Rajadhyaksha M

    更新日期:2019-05-08 00:00:00

  • In vivo autofluorescence spectroscopy of oral premalignant and malignant lesions: distortion of fluorescence intensity by submucous fibrosis.

    abstract:BACKGROUND AND OBJECTIVES:To test whether autofluorescence spectroscopy can be used for the diagnosis of oral neoplasia in a high-risk population, we characterized the in vivo autofluorescence spectra from oral submucous fibrosis (OSF) lesions and oral premalignant and malignant lesions in both OSF and non-OSF patients...

    journal_title:Lasers in surgery and medicine

    pub_type: 杂志文章

    doi:10.1002/lsm.10180

    authors: Tsai T,Chen HM,Wang CY,Tsai JC,Chen CT,Chiang CP

    更新日期:2003-01-01 00:00:00

  • Prospective study of cutaneous phototoxicity after systemic hematoporphyrin derivative.

    abstract::Hematoporphyrin derivative (HpD) is a photoactive, oncophilic substance that produces cutaneous photosensitivity as its only significant side effect. Twenty-three patients who received systemic HpD and the usual light-avoidance precautions were studied prospectively to determine the incidence and severity of cutaneous...

    journal_title:Lasers in surgery and medicine

    pub_type: 杂志文章

    doi:10.1002/lsm.1900080312

    authors: Wooten RS,Smith KC,Ahlquist DA,Muller SA,Balm RK

    更新日期:1988-01-01 00:00:00

  • Hollow-waveguide-based nanosecond, near-infrared pulsed laser ablation of tissue.

    abstract:BACKGROUND AND OBJECTIVE:Short-pulse solid-state lasers have recently received much attention as new coherent light sources for medical applications, but steady transmission of their high-energy output pulses through a solid quartz fiber is difficult because of the onset of laser-induced breakdown. We previously demons...

    journal_title:Lasers in surgery and medicine

    pub_type: 杂志文章

    doi:10.1002/lsm.20210

    authors: Sato S,Shi YW,Matsuura Y,Miyagi M,Ashida H

    更新日期:2005-08-01 00:00:00

  • Experience treating earlobe keloids with laser diode 980 nm excision followed by triamcinolone infiltration.

    abstract:BACKGROUND AND OBJECTIVE:Lasers have a favorable synergistic effect when combined with other modalities of treatment against keloids. Different types of lasers have been used with triamcinolone infiltration, resulting in promising success rates. The purpose of this study is to describe our first experience treating ear...

    journal_title:Lasers in surgery and medicine

    pub_type: 杂志文章

    doi:10.1002/lsm.23310

    authors: Fuenmayor P,Quiñonez H,Salas R,Pujadas Z

    更新日期:2020-08-22 00:00:00

  • Electronic device generated light increases reactive oxygen species in human fibroblasts.

    abstract:OBJECTIVES:Our skin is constantly exposed to light from solar radiation and electronic devices, which impact skin physiology and aging. The biological altering properties of ultraviolet (UV) solar radiation on skin have been well established. There is significant scientific and public interest on the effects of electro...

    journal_title:Lasers in surgery and medicine

    pub_type: 杂志文章

    doi:10.1002/lsm.22794

    authors: Austin E,Huang A,Adar T,Wang E,Jagdeo J

    更新日期:2018-02-05 00:00:00

  • Intradermally focused infrared laser pulses: thermal effects at defined tissue depths.

    abstract:BACKGROUND AND OBJECTIVES:To produce controlled, spatially confined thermal effects in dermis. STUDY DESIGNS/MATERIALS AND METHODS:A 1 W, 1,500 nm fiber-coupled diode laser was focused with a high numerical aperture (NA) objective to achieve a tight optical focus within the upper dermis of skin held in contact with a ...

    journal_title:Lasers in surgery and medicine

    pub_type: 杂志文章

    doi:10.1002/lsm.20142

    authors: Khan MH,Sink RK,Manstein D,Eimerl D,Anderson RR

    更新日期:2005-04-01 00:00:00

  • Pulsed dye laser therapy for sun damaged skin.

    abstract:OBJECTIVE:The objective of this study was to evaluate the effectiveness of the pulsed dye laser (585 nm, 450 ms) in the treatment of sun induced wrinkles. DESIGN:Patients had one pulsed dye laser (585 nm) treatment. The treated areas were assessed by the following methods: grading of skin wrinkles at 6 weeks, 12 weeks...

    journal_title:Lasers in surgery and medicine

    pub_type: 杂志文章

    doi:10.1002/(sici)1096-9101(1999)25:3<229::aid-lsm7>3.

    authors: Zelickson BD,Kilmer SL,Bernstein E,Chotzen VA,Dock J,Mehregan D,Coles C

    更新日期:1999-01-01 00:00:00

  • Healing process of laser-welded intestinal anastomosis.

    abstract::Intestinal welding by means of low-power laser has been reported as an efficient method for intestinal anastomosis. We designed an experimental model in rats to investigate collagen and DNA concentrations in CO2 laser-welded anastomoses as compared with those in sutured anastomoses on the 4th, 7th, and 10th postoperat...

    journal_title:Lasers in surgery and medicine

    pub_type: 杂志文章

    doi:10.1002/lsm.1900140106

    authors: Rabau MY,Wasserman I,Shoshan S

    更新日期:1994-01-01 00:00:00