Abstract:
BACKGROUND AND OBJECTIVE:Low-intensity laser irradiation (LILI) has been used to modulate a variety of biological processes, including diabetic wound healing. The mechanism of action is thought to exist primarily with the mitochondria. This study aimed to determine the effect of irradiation on normal, diabetic, and ischemic mitochondrial electron transport chain (ETC) complexes. MATERIALS AND METHODS:Normal, diabetic and ischemic human skin fibroblast mitochondria were irradiated in vitro at a wavelength of 660 nm and a fluence of either 5 or 15 J/cm(2). Non-irradiated mitochondria served as controls. Enzyme activities of mitochondrial complexes I, II, III, and IV were determined immediately post-irradiation. Normal, diabetic, and ischemic cells were irradiated and adenosine triphosphate (ATP) and active mitochondria were determined by luminescence and fluorescent microscopy, respectively. RESULTS:Irradiated diabetic mitochondria at a fluence of 15 J/cm(2) showed a significant decrease in complex III activity (P < 0.05). Normal (P < 0.01) and diabetic (P < 0.05) mitochondria irradiated at either 5 or 15 J/cm(2) showed a significant increase in complex IV activity. ATP results showed a significant increase in irradiated normal cells (5 J/cm(2); P < 0.05) and diabetic cells (15 J/cm(2); P < 0.01). There was a higher accumulation of active mitochondria in irradiated cells than non-irradiated cells. CONCLUSION:Irradiation at 660 nm has the ability to influence mitochondrial enzyme activity, in particular cytochrome c oxidase. This leads to increased mitochondrial activity and ATP synthesis.
journal_name
Lasers Surg Medjournal_title
Lasers in surgery and medicineauthors
Houreld NN,Masha RT,Abrahamse Hdoi
10.1002/lsm.22027subject
Has Abstractpub_date
2012-07-01 00:00:00pages
429-34issue
5eissn
0196-8092issn
1096-9101journal_volume
44pub_type
杂志文章abstract:OBJECTIVES:Cryolipolysis of the arms has been demonstrated to be an effective treatment for non-invasive reduction of subcutaneous fat. This study evaluated the safety and efficacy of the concurrent use of a new commercially-available small applicator in conjunction with an existing medium sized applicator for the cust...
journal_title:Lasers in surgery and medicine
pub_type: 杂志文章
doi:10.1002/lsm.22811
更新日期:2018-03-22 00:00:00
abstract::This work presents measurements of time-resolved fluorescence microscopy of hematoporphyrin-derivative (HpD) in single cells of mice tissue (both tumor and normal cells), in HeLa Cells, and in solution. The measurements were performed using a pulsed-laser microfluorometer with high spatial and temporal resolution. In ...
journal_title:Lasers in surgery and medicine
pub_type: 杂志文章
doi:10.1002/lsm.1900020103
更新日期:1982-01-01 00:00:00
abstract:BACKGROUND AND OBJECTIVES:The conventional pulsed-dye laser (wavelength 585 nm, pulse duration 0.5 milliseconds) is seen as the standard treatment for port wine stains (PWS). Using the pulsed-dye laser at wavelengths of 590, 595, and 600 nm and at varying pulse durations of 1.5-40 milliseconds is one of the newest deve...
journal_title:Lasers in surgery and medicine
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1002/lsm.20003
更新日期:2004-01-01 00:00:00
abstract:BACKGROUND AND OBJECTIVE:A novel, noninvasive technology, utilizing suction-coupled radiofrequency (RF) heating and ultra-short pulse duration, high-voltage electrical pulses was studied for its efficacy and safety on adipose tissue reduction. METHODS:Twenty-one subjects underwent treatment of their abdominal fat once...
journal_title:Lasers in surgery and medicine
pub_type: 临床试验,杂志文章
doi:10.1002/lsm.22223
更新日期:2014-02-01 00:00:00
abstract:BACKGROUND AND OBJECTIVES:While cryolipolysis initially received FDA clearance for fat reduction in the abdomen and flanks, there was significant interest in non-surgical fat reduction for other sites, such as the inner and outer thighs. This article reports the results of an inner thigh study which contributed to FDA ...
journal_title:Lasers in surgery and medicine
pub_type: 临床试验,杂志文章,多中心研究
doi:10.1002/lsm.22320
更新日期:2015-02-01 00:00:00
abstract:BACKGROUND AND OBJECTIVE:The aim was to investigate the bactericidal effect of the 1,064 nm Nd:YAG laser on Staphylococcus epidermidis. MATERIAL AND METHODS:S. epidermidis was inoculated on agar plates and then exposed to pulsed laser light in three different modes: with an uninterrupted train of pulses, or with two d...
journal_title:Lasers in surgery and medicine
pub_type: 杂志文章
doi:10.1002/1096-9101(2000)27:4<336::aid-lsm6>3.0.co;2
更新日期:2000-01-01 00:00:00
abstract:BACKGROUND AND OBJECTIVES:Facial teleangectasias can be a relevant cosmetic problem, which the patient usually hides with a thick layer of makeup. This study will describe the response on these vascular lesions using the intense pulsed light (IPL) source. METHODS:Five hundred eighteen consecutive patients were treated...
journal_title:Lasers in surgery and medicine
pub_type: 杂志文章
doi:10.1002/lsm.20187
更新日期:2005-07-01 00:00:00
abstract::This study was designed to determine the optimal laser power (watts) for maximal strength of argon laser vascular fusions by measuring bursting pressures of repairs. Longitudinal incisions (n = 105) measuring either 2.5 or 5 mm in length were performed in canine femoral, carotid, and jugular veins and arteries and wer...
journal_title:Lasers in surgery and medicine
pub_type: 杂志文章
doi:10.1002/lsm.1900090509
更新日期:1989-01-01 00:00:00
abstract:BACKGROUND AND OBJECTIVE:Phototherapy plus dynamic exercise can enhance physical performance and improve health. The aim of our study was to evaluate the effect of low-level laser therapy (LLLT) associated with high intensity resistance training (HIT) on cardiac autonomic and muscle metabolic responses in rats. STUDY ...
journal_title:Lasers in surgery and medicine
pub_type: 杂志文章
doi:10.1002/lsm.22298
更新日期:2014-12-01 00:00:00
abstract::We examined the biostimulating effects of helium-neon laser radiation (HeNe; 632.8 nm), pulsed infrared laser radiation (IR; 904 nm), and the two combined on skin wound healing in New Zealand white rabbits. Seventy-two rabbits received either 1) no exposure, 2) 1.65 J/cm2 HeNe, 3) 8.25 J/cm2 pulsed IR, or 4) both HeNe...
journal_title:Lasers in surgery and medicine
pub_type: 杂志文章
doi:10.1002/lsm.1900090111
更新日期:1989-01-01 00:00:00
abstract:BACKGROUND AND OBJECTIVE:During endoscopic sinus surgery (ESS), intra-operative bleeding can significantly compromise visualization of the surgical field. The diode laser that provides good hemostatic and vaporization effects and excellent photocoagulation has been successfully applied in endoscopic surgery with severa...
journal_title:Lasers in surgery and medicine
pub_type: 杂志文章
doi:10.1002/lsm.22340
更新日期:2015-03-01 00:00:00
abstract:BACKGROUND AND OBJECTIVE:Progressive ascending telangiectasia (PAT) is a distinct entity with telangiectatic superficial vessels on lower extremities as its main clinical feature. A relationship with occult infections and response to antibiotic and antifungal drugs have been described, although not all cases can be suc...
journal_title:Lasers in surgery and medicine
pub_type: 杂志文章
doi:10.1002/(sici)1096-9101(1997)21:5<413::aid-lsm1>3.
更新日期:1997-01-01 00:00:00
abstract:INTRODUCTION:Patient demand for non-surgical, non-invasive, and no-downtime wrinkle reduction treatment procedures has grown dramatically over the past decade as new treatments and technologies have been introduced. This study was performed in order to evaluate the safety and efficacy of the TriPollar radiofrequency (R...
journal_title:Lasers in surgery and medicine
pub_type: 杂志文章
doi:10.1002/lsm.22044
更新日期:2012-08-01 00:00:00
abstract:BACKGROUND AND OBJECTIVE:Interstitial photodynamic therapy (iPDT) of non-resectable recurrent glioblastoma using 5-aminolevulinic acid (ALA)-induced protoporphyrin IX (PpIX) has shown a promising outcome. It remained unclear, however, to what extent inter- and intra-tumoural differences of PpIX concentrations influence...
journal_title:Lasers in surgery and medicine
pub_type: 临床试验,杂志文章
doi:10.1002/lsm.22126
更新日期:2013-04-01 00:00:00
abstract::Our experiments on transmitting high-power excimer laser pulses through optical fibers and our investigations on excimer laser ablation of hard tissue show the feasibility of using the excimer laser as an additional instrument in general and accident surgery involving minimal invasive surgery. By combining XeCl-excime...
journal_title:Lasers in surgery and medicine
pub_type: 杂志文章
doi:10.1002/lsm.1900110612
更新日期:1991-01-01 00:00:00
abstract:BACKGROUND AND OBJECTIVE:Recent studies have sought to utilize diode laser "micropulsing" in order to preserve therapeutic efficacy of retinal photocoagulation while minimizing pain and subjacent tissue injury. A model for the transient thermal tissue response to continuous and micropulsed diode laser output is present...
journal_title:Lasers in surgery and medicine
pub_type: 杂志文章
doi:10.1002/(sici)1096-9101(1997)20:4<409::aid-lsm6>3.
更新日期:1997-01-01 00:00:00
abstract:BACKGROUND:Reflectance confocal microscopy (RCM) is a developing approach for noninvasive detection of oral lesions with label-free contrast and cellular-level resolution. For access into the oral cavity, confocal microscopes are being configured with small-diameter telescopic probes and small objective lenses. However...
journal_title:Lasers in surgery and medicine
pub_type: 杂志文章
doi:10.1002/lsm.23090
更新日期:2019-05-08 00:00:00
abstract:OBJECTIVE:Acute radiodermatitis (RD) is a distressing and painful skin reaction that occurs in 95% of the patients undergoing radiotherapy (RT). The aim of this study was to evaluate the effectiveness of photobiomodulation therapy (PBMT) in the prevention of acute RD in breast cancer (BC) patients undergoing RT. METHO...
journal_title:Lasers in surgery and medicine
pub_type: 杂志文章
doi:10.1002/lsm.22804
更新日期:2018-02-10 00:00:00
abstract::The healing of standardized fracture of rabbit radius was expedited as a result of treatment with low-power CO2 laser irradiation. Observation with transmission electron microscope revealed the following favorable effects of CO2 laser irradiation: The red blood cells were induced to disintegrate, thus promoting the ab...
journal_title:Lasers in surgery and medicine
pub_type: 杂志文章
doi:10.1002/lsm.1900060309
更新日期:1986-01-01 00:00:00
abstract::This study examined both the effect of variations in optical fiber tip and in light wavelength on laser-induced hyperthermia in rat brain. Normal rat brains were exposed to argon laser light (454-514.5 nm) delivered through an intracerebral end-emitting (bare-tipped) or a diffusion-emitting (sapphire-tipped) optical f...
journal_title:Lasers in surgery and medicine
pub_type: 杂志文章
doi:10.1002/lsm.1900070413
更新日期:1987-01-01 00:00:00
abstract::The effects of laser energy on articular cartilage were studied utilizing the neodymium YAG laser. Partial-thickness cartilage defects were surgically attempted in the femoral condyles of knee joints in guinea pigs. The defects were exposed to laser energy of varying intensities [group I, 25 J (5 W X 5 sec); group II,...
journal_title:Lasers in surgery and medicine
pub_type: 杂志文章
doi:10.1002/lsm.1900050606
更新日期:1985-01-01 00:00:00
abstract:BACKGROUND AND OBJECTIVES:Many studies have investigated the application of micro-insulated needles with radio frequency (RF) to treat acne in humans; however, the use of a micro-insulated needle RF applicator has not yet been studied in an animal model. The purpose of this study was to evaluate the effectiveness of a ...
journal_title:Lasers in surgery and medicine
pub_type: 杂志文章
doi:10.1002/lsm.22599
更新日期:2017-04-01 00:00:00
abstract:BACKGROUND AND OBJECTIVES:This preliminary report describes a new laser-assisted treatment option for the emerging complication of bisphosphonate related osteonecrosis (BON) of the jaw. MATERIALS AND METHODS:In eight tumour patients (three women, five men) ten bony lesions in the maxilla and mandible in the course of ...
journal_title:Lasers in surgery and medicine
pub_type: 临床试验,杂志文章
doi:10.1002/lsm.20730
更新日期:2009-01-01 00:00:00
abstract:BACKGROUND AND OBJECTIVE:Lasers are used for caries removal in enamel and dentin, but are currently limited in their ability to remove sound tooth structure. In this study plasma interaction regimes are investigated as the principal factor determining the ablation characteristics. STUDY DESIGN/MATERIALS AND METHODS:Th...
journal_title:Lasers in surgery and medicine
pub_type: 杂志文章
doi:10.1002/(SICI)1096-9101(1996)18:2<121::AID-LSM1>3.
更新日期:1996-01-01 00:00:00
abstract:BACKGROUND AND OBJECTIVES:Smear layer removal with EDTA from root canal walls allows greater cleaning and disinfection of root canals. However, because Er:YAG laser acts on the removal of the smear layer, the objective of investigation was to analyze in vitro the effect of Er:YAG laser on dentin root canal wall permeab...
journal_title:Lasers in surgery and medicine
pub_type: 杂志文章
doi:10.1002/(sici)1096-9101(2000)26:3<277::aid-lsm5>3.
更新日期:2000-01-01 00:00:00
abstract:BACKGROUND AND OBJECTIVE:Fifty percent human albumin solder significantly improves weld strength when compared to lower concentrations [Wright et al., ASLMS meeting, April, 1995]. We developed a method for preparing 50% human albumin that may be considered compatible for clinical applications. STUDY DESIGN/MATERIALS A...
journal_title:Lasers in surgery and medicine
pub_type: 杂志文章
doi:10.1002/(SICI)1096-9101(1996)19:1<2::AID-LSM1>3.0.
更新日期:1996-01-01 00:00:00
abstract::Percutaneous endoscopic approaches to cholelithiasis would be facilitated by methods for welding gallbladder tissues. We evaluated the bursting pressure and histologic appearance of canine gallbladder tissue welded with a thulium-holmium-chromium:YAG (THC:YAG) laser producing a 2.15 microns pulsed output. The tissue a...
journal_title:Lasers in surgery and medicine
pub_type: 杂志文章
doi:10.1002/lsm.1900090211
更新日期:1989-01-01 00:00:00
abstract:BACKGROUND AND OBJECTIVES:Nonmelanoma skin cancers, including basal cell carcinoma (BCC) and squamous cell carcinoma (SCC), are the most common skin cancers, presenting nearly as many cases as all other cancers combined. The current gold-standard for clinical diagnosis of these lesions is histopathologic examination, a...
journal_title:Lasers in surgery and medicine
pub_type: 杂志文章
doi:10.1002/lsm.20653
更新日期:2008-09-01 00:00:00
abstract:BACKGROUND AND OBJECTIVE:Photodynamic therapy (PDT) has been used as a therapeutic alternative to treat periodontitis, especially in challenging sites that require additional periodontal therapy such as residual pockets. The aim of this split-mouth randomized trial was to evaluate the microbiological and clinical effec...
journal_title:Lasers in surgery and medicine
pub_type: 杂志文章,随机对照试验
doi:10.1002/lsm.22449
更新日期:2016-12-01 00:00:00
abstract:OBJECTIVES:Photodynamic therapy (PDT) is an effective cancer treatment that uses photosensitizers, light, and oxygen to destroy malignant cells. Porphyrins, and in particular the cationic derivatives, are the most investigated photosensitizers for PDT. In this context, it is important to study new methodologies to deve...
journal_title:Lasers in surgery and medicine
pub_type: 杂志文章
doi:10.1002/lsm.22824
更新日期:2018-07-01 00:00:00