Abstract:
:This work presents measurements of time-resolved fluorescence microscopy of hematoporphyrin-derivative (HpD) in single cells of mice tissue (both tumor and normal cells), in HeLa Cells, and in solution. The measurements were performed using a pulsed-laser microfluorometer with high spatial and temporal resolution. In agreement with the results obtained with other techniques, it has been found that the tumor cells examined present an HpD uptake about five times higher than that of the normal cells of the corresponding tissue and that, within a cell, HpD become localized mainly in the cytoplasm. It has also been found that the fluorescence decay time is different in cells as compared with solution, and that the presence of HpD stabilizes cell auto-fluorescence. These results are discussed.
journal_name
Lasers Surg Medjournal_title
Lasers in surgery and medicineauthors
Docchio F,Ramponi R,Sacchi CA,Bottiroli G,Freitas Idoi
10.1002/lsm.1900020103subject
Has Abstractpub_date
1982-01-01 00:00:00pages
21-8issue
1eissn
0196-8092issn
1096-9101journal_volume
2pub_type
杂志文章abstract::Thirty-six deep hepatic lesions of localized photocoagulation were induced in 11 pigs by means of a neodymium-YAG laser. Laser applications of 80 W/10 sec (10.190 W/cm2) were transmitted through a handpiece coupled to a water-cooling circulation system to protect the quartz fiber and positioned through an echo-guided ...
journal_title:Lasers in surgery and medicine
pub_type: 杂志文章
doi:10.1002/lsm.1900080509
更新日期:1988-01-01 00:00:00
abstract::Photochemical internalization (PCI) is a method for intracellular delivery of hydrophilic macromolecular drugs with intracellular targets as well as other drugs with limited ability to penetrate cellular membranes. Such drugs enter cells by means of endocytosis and are to a large extent degraded by hydrolytic enzymes ...
journal_title:Lasers in surgery and medicine
pub_type: 杂志文章,评审
doi:10.1002/lsm.21084
更新日期:2011-09-01 00:00:00
abstract:BACKGROUND AND OBJECTIVES:Complete neurosurgical resection of intracranial meningiomas is essential to avoid residual tumor tissue and thus minimize the risk of tumor recurrence. However, local recurrence of meningiomas is not uncommon mainly due to insufficient intraoperative detection of residual tumor tissue within ...
journal_title:Lasers in surgery and medicine
pub_type: 杂志文章
doi:10.1002/lsm.23294
更新日期:2020-07-01 00:00:00
abstract:BACKGROUND AND OBJECTIVE:Erbium lasers are potential tools to remove caries and dental hard tissue but bond strengths of composites to those preparations are reported to be lower than conventional methods. The purpose of this study was to evaluate the effect of mechanical excavation and/or chemical alteration on bond s...
journal_title:Lasers in surgery and medicine
pub_type: 杂志文章
doi:10.1002/lsm.20790
更新日期:2009-08-01 00:00:00
abstract:BACKGROUND AND OBJECTIVE:The relationship between tissue ablation volume and the formation of insoluble gas of the currently available excimer laser systems is unknown. This aspect was evaluated in two excimer laser systems. STUDY DESIGN/MATERIALS AND METHODS:We measured tissue ablation volume and gas production of tw...
journal_title:Lasers in surgery and medicine
pub_type: 杂志文章
doi:10.1002/(SICI)1096-9101(1996)18:2<197::AID-LSM11>3
更新日期:1996-01-01 00:00:00
abstract::One carotid from each dog underwent a laser anastomosis and the other carotid an interrupted suture repair. One or eight weeks later (n greater than or equal to 4 dogs/time period); four rings (1 mm in length) containing the laser or suture anastomosis or the normal artery (two/dog) were removed. Using a photoelectric...
journal_title:Lasers in surgery and medicine
pub_type: 杂志文章
doi:10.1002/lsm.1900120213
更新日期:1992-01-01 00:00:00
abstract:BACKGROUND AND OBJECTIVES:We evaluated the potential of combining laser excision with laser interstitial hyperthermia in order to improve the quality of life of patients with 3rd or 4th stage malignant head and neck tumours. STUDY DESIGN/MATERIALS AND METHODS:To evaluate the quality of life, we used the 4th version of...
journal_title:Lasers in surgery and medicine
pub_type: 杂志文章
doi:10.1002/lsm.10253
更新日期:2004-01-01 00:00:00
abstract:BACKGROUND AND OBJECTIVES:The risks of currently available invasive procedures in body contouring motivate a need for safer, non-invasive technologies for improving the appearance of body silhouette. A new device has been developed that uses focused therapeutic ultrasound to reduce adipose tissue non-invasively. The ai...
journal_title:Lasers in surgery and medicine
pub_type: 临床试验,杂志文章
doi:10.1002/lsm.20478
更新日期:2007-04-01 00:00:00
abstract::Seven cases of cerebral tumors (four deep-seated sovratentorial gliomas located in motor area, occipital region, parietal region and frontal region; one deep-seated cystic cerebellar spongioblastoma; one sphenoidal wing meningioma, and one spinal cord intradural tumor) were simultaneously irradiated in the same area w...
journal_title:Lasers in surgery and medicine
pub_type: 杂志文章
doi:10.1002/lsm.1900020205
更新日期:1982-01-01 00:00:00
abstract:BACKGROUND:We sought to investigate risk factors for recurrence of vaginal intraepithelial neoplasia (VAIN) in the vaginal vault after laser vaporization. MATERIALS AND METHODS:Among 162 patients treated with laser vaporization in the vaginal vault after hysterectomy between March 1998 and August 2007, the clinical da...
journal_title:Lasers in surgery and medicine
pub_type: 杂志文章
doi:10.1002/lsm.20741
更新日期:2009-03-01 00:00:00
abstract:BACKGROUND:Port wine stains (PWS) affect 0.3-0.5% of both sexes of newborns, usually occurring on the face. OBJECTIVE:To document safety and effectiveness of cryogen spray cooled, pulsed-dye laser at higher fluences than previously used to lighten facial PWS in infants < or =6 months, and establish that frequent treat...
journal_title:Lasers in surgery and medicine
pub_type: 杂志文章
doi:10.1002/lsm.20529
更新日期:2007-08-01 00:00:00
abstract:BACKGROUND AND OBJECTIVE:In previous investigations, small variations in the energy densities of low level light therapy (LLLT) were found to produce significant differences in the proliferation of resting T-lymphocytes in vitro. Pulsing these cells with mitogen in addition to laser therapy produced inhibitory effects ...
journal_title:Lasers in surgery and medicine
pub_type: 杂志文章
doi:10.1002/(sici)1096-9101(2000)26:4<357::aid-lsm3>3.
更新日期:2000-01-01 00:00:00
abstract:UNLABELLED:BACKGROUND AND OBJECTIVE To examine the biophysical effects of photothermal heating on herniated intervertebral discs during laser decompression surgery. STUDY DESIGN/MATERIALS AND METHODS:Ex vivo bovine nucleus pulposus specimens were irradiated with a Nd: YAG laser (lambda = 1.32 microm, 100 seconds expos...
journal_title:Lasers in surgery and medicine
pub_type: 杂志文章
doi:10.1002/lsm.1046
更新日期:2001-01-01 00:00:00
abstract:OBJECTIVE:The aim was to evaluate the influence of Er,Cr:YSGG laser irradiation associated with different final irrigation protocols on the bond strength of epoxy resin-based root canal sealer to root dentin, on the dentin/filling material interface and in the temperature variation during irradiation. METHODOLOGY:Nine...
journal_title:Lasers in surgery and medicine
pub_type: 杂志文章
doi:10.1002/lsm.22496
更新日期:2016-12-01 00:00:00
abstract:BACKGROUND AND OBJECTIVES:Nitrogen laser-induced fluorescence (LIF) spectra of sound tooth consists of two broad bands centered at 440 and 490 nm, with two apparent side bands on either side. In order to locate the exact peak position of these bands and to effectively utilize the LIF spectral signatures for detection o...
journal_title:Lasers in surgery and medicine
pub_type: 杂志文章
doi:10.1002/lsm.20229
更新日期:2005-10-01 00:00:00
abstract:BACKGROUND AND OBJECTIVE:The Holmium: YAG (Ho: YAG) laser has been used for the ablation of prolapsed discs but alternative techniques are available, and this application remains controversial. It also has potential for the decompression of nerve roots within narrowed foraminae with the technique of endoscopic laser fo...
journal_title:Lasers in surgery and medicine
pub_type: 杂志文章
doi:10.1002/lsm.1083
更新日期:2001-01-01 00:00:00
abstract:BACKGROUND AND OBJECTIVE:The pulsed holmium laser is a promising tool for tissue ablation but possesses some limitations. For example, it is capable of producing significant mechanical damage in certain tissues in the form of fissures and fractures. Because longer pulse durations should reduce mechanical damage, this s...
journal_title:Lasers in surgery and medicine
pub_type: 杂志文章
doi:10.1002/(SICI)1096-9101(1996)19:1<97::AID-LSM11>3.
更新日期:1996-01-01 00:00:00
abstract:BACKGROUND AND OBJECTIVES:One of the methods to detect and localize tumors in tissue is to use fluorophore conjugated specific antibodies as tumor surface markers. The goals of this study are to understand and quantify the pharmacokinetics of fluorophore conjugated antibodies in the vicinity of a tumor. This study conc...
journal_title:Lasers in surgery and medicine
pub_type: 杂志文章
doi:10.1002/lsm.20200
更新日期:2005-08-01 00:00:00
abstract::Low power laser irradiation has been reported to cause biological effects due to the photochemical and/or photophysical action of the radiation. This study determined quantitatively if transcutaneous low power laser irradiation can affect the regeneration of the rat facial nerve. The facial nerve was crushed unilatera...
journal_title:Lasers in surgery and medicine
pub_type: 杂志文章
doi:10.1002/lsm.1900130113
更新日期:1993-01-01 00:00:00
abstract:BACKGROUND AND OBJECTIVES:To determine the true efficacy of the 308-nm excimer laser for the treatment of vitiligo, while taking into account confounding factors such as anatomic site of treatment, age, sex, skin type, MED, and duration of evolution of the vitiligo. STUDY DESIGN/MATERIALS AND METHODS:Thirty-five patie...
journal_title:Lasers in surgery and medicine
pub_type: 临床试验,杂志文章
doi:10.1002/lsm.20057
更新日期:2004-01-01 00:00:00
abstract:BACKGROUND AND OBJECTIVES:Porcine skin is a widely used model in diffusion studies, but its usefulness for laser-assisted drug delivery (LADD) has not been evaluated in comparison with human skin. This study compared porcine and human skin in ex vivo LADD diffusion studies. STUDY DESIGN/MATERIALS AND METHODS:Ex vivo a...
journal_title:Lasers in surgery and medicine
pub_type:
doi:10.1002/lsm.23344
更新日期:2020-11-08 00:00:00
abstract:BACKGROUND AND OBJECTIVES:Attempts at using the pulsed dye laser (PDL) operating at 585 nm wavelength and 0.45 milliseconds pulse duration to treat leg veins have been notably unsuccessful. The aim of this study is to evaluate the efficacy and complications of the 595 nm long PDL in the treatment of leg veins. STUDY D...
journal_title:Lasers in surgery and medicine
pub_type: 杂志文章
doi:10.1002/lsm.20035
更新日期:2004-01-01 00:00:00
abstract::Interstitial thermotherapy using Nd:YAG-laser induced hyperthermia is a new stereotactic method for the treatment of brain tumors in poorly accessible regions. To provide a basis for the underlying tissue alterations, we have analyzed the spatial and temporal pattern of interstitial laser hyperthermia lesions in the n...
journal_title:Lasers in surgery and medicine
pub_type: 杂志文章
doi:10.1002/lsm.1900130212
更新日期:1993-01-01 00:00:00
abstract:BACKGROUND AND OBJECTIVES:The conventional pulsed-dye laser (wavelength 585 nm, pulse duration 0.5 milliseconds) is seen as the standard treatment for port wine stains (PWS). Using the pulsed-dye laser at wavelengths of 590, 595, and 600 nm and at varying pulse durations of 1.5-40 milliseconds is one of the newest deve...
journal_title:Lasers in surgery and medicine
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1002/lsm.20003
更新日期:2004-01-01 00:00:00
abstract:BACKGROUND:Patients suffering from malignant gliomas have a poor prognosis. For the surgical treatment of these tumors, 5-aminolevulinic acid (5-ALA) has become a new standard. AIMS:This review intends to provide an overview over current status, significance, limitations, and future perspectives of 5-ALA based fluores...
journal_title:Lasers in surgery and medicine
pub_type: 杂志文章,评审
doi:10.1002/lsm.22933
更新日期:2018-07-01 00:00:00
abstract::We examined the biostimulating effects of helium-neon laser radiation (HeNe; 632.8 nm), pulsed infrared laser radiation (IR; 904 nm), and the two combined on skin wound healing in New Zealand white rabbits. Seventy-two rabbits received either 1) no exposure, 2) 1.65 J/cm2 HeNe, 3) 8.25 J/cm2 pulsed IR, or 4) both HeNe...
journal_title:Lasers in surgery and medicine
pub_type: 杂志文章
doi:10.1002/lsm.1900090111
更新日期:1989-01-01 00:00:00
abstract:BACKGROUND AND OBJECTIVE:Basal cell carcinoma (BCC) is an indolent form of skin cancer that is rarely life threatening, but can cause significant cosmetic and functional morbidity. Surgical treatments often result in disfiguring scars, while topical therapies frequently result in recurrence. The need for a more effecti...
journal_title:Lasers in surgery and medicine
pub_type: 杂志文章
doi:10.1002/lsm.22803
更新日期:2018-02-13 00:00:00
abstract:BACKGROUND AND OBJECTIVE:Short pulsed and scanned CO(2) lasers that target water molecules are currently used for cutaneous resurfacing. These CO(2) resurfacing lasers produce acute cutaneous contraction, which can be quantitated as a measure of the laser's effect. We postulated that targeting the vibrational and rotat...
journal_title:Lasers in surgery and medicine
pub_type: 杂志文章
doi:10.1002/(sici)1096-9101(1999)25:1<1::aid-lsm1>3.0.
更新日期:1999-01-01 00:00:00
abstract:BACKGROUND AND OBJECTIVE:The extent of thermal injury during laser prostatectomy is dependent on the light distribution in laser-irradiated tissue. As tissue is irradiated, the optical properties change as a function of temperature due to an alteration of molecular and cellular structure. The purpose of the present stu...
journal_title:Lasers in surgery and medicine
pub_type: 杂志文章
doi:10.1002/(sici)1096-9101(1999)24:1<38::aid-lsm7>3.0
更新日期:1999-01-01 00:00:00
abstract::Histological investigation of the dog's myocardium performed 7 and 25 days after irradiation with different doses of Nd-YAG laser show that the damage zone has the shape of a hollow sphere with minor changes in cardiomyocytes in the central part of the sphere. The small blood vessels in the irradiated zone remained un...
journal_title:Lasers in surgery and medicine
pub_type: 杂志文章
doi:10.1002/lsm.1900050506
更新日期:1985-01-01 00:00:00