Time-resolved fluorescence microscopy of hematoporphyrin-derivative in cells.

Abstract:

:This work presents measurements of time-resolved fluorescence microscopy of hematoporphyrin-derivative (HpD) in single cells of mice tissue (both tumor and normal cells), in HeLa Cells, and in solution. The measurements were performed using a pulsed-laser microfluorometer with high spatial and temporal resolution. In agreement with the results obtained with other techniques, it has been found that the tumor cells examined present an HpD uptake about five times higher than that of the normal cells of the corresponding tissue and that, within a cell, HpD become localized mainly in the cytoplasm. It has also been found that the fluorescence decay time is different in cells as compared with solution, and that the presence of HpD stabilizes cell auto-fluorescence. These results are discussed.

journal_name

Lasers Surg Med

authors

Docchio F,Ramponi R,Sacchi CA,Bottiroli G,Freitas I

doi

10.1002/lsm.1900020103

subject

Has Abstract

pub_date

1982-01-01 00:00:00

pages

21-8

issue

1

eissn

0196-8092

issn

1096-9101

journal_volume

2

pub_type

杂志文章
  • Deep localized neodymium (Nd)-YAG laser photocoagulation in liver using a new water cooled and echoguided handpiece.

    abstract::Thirty-six deep hepatic lesions of localized photocoagulation were induced in 11 pigs by means of a neodymium-YAG laser. Laser applications of 80 W/10 sec (10.190 W/cm2) were transmitted through a handpiece coupled to a water-cooling circulation system to protect the quartz fiber and positioned through an echo-guided ...

    journal_title:Lasers in surgery and medicine

    pub_type: 杂志文章

    doi:10.1002/lsm.1900080509

    authors: Godlewski G,Rouy S,Pignodel C,Ould-Said H,Eledjam JJ,Bourgeois JM,Sambuc P

    更新日期:1988-01-01 00:00:00

  • Photochemical internalization of tumor-targeted protein toxins.

    abstract::Photochemical internalization (PCI) is a method for intracellular delivery of hydrophilic macromolecular drugs with intracellular targets as well as other drugs with limited ability to penetrate cellular membranes. Such drugs enter cells by means of endocytosis and are to a large extent degraded by hydrolytic enzymes ...

    journal_title:Lasers in surgery and medicine

    pub_type: 杂志文章,评审

    doi:10.1002/lsm.21084

    authors: Weyergang A,Selbo PK,Berstad ME,Bostad M,Berg K

    更新日期:2011-09-01 00:00:00

  • High Diagnostic Accuracy of Visible 5-ALA Fluorescence in Meningioma Surgery According to Histopathological Analysis of Tumor Bulk and Peritumoral Tissue.

    abstract:BACKGROUND AND OBJECTIVES:Complete neurosurgical resection of intracranial meningiomas is essential to avoid residual tumor tissue and thus minimize the risk of tumor recurrence. However, local recurrence of meningiomas is not uncommon mainly due to insufficient intraoperative detection of residual tumor tissue within ...

    journal_title:Lasers in surgery and medicine

    pub_type: 杂志文章

    doi:10.1002/lsm.23294

    authors: Wadiura LI,Millesi M,Makolli J,Wais J,Kiesel B,Mischkulnig M,Mercea PA,Roetzer T,Knosp E,Rössler K,Widhalm G

    更新日期:2020-07-01 00:00:00

  • Enhancement of bonding to enamel and dentin prepared by Er,Cr:YSGG laser.

    abstract:BACKGROUND AND OBJECTIVE:Erbium lasers are potential tools to remove caries and dental hard tissue but bond strengths of composites to those preparations are reported to be lower than conventional methods. The purpose of this study was to evaluate the effect of mechanical excavation and/or chemical alteration on bond s...

    journal_title:Lasers in surgery and medicine

    pub_type: 杂志文章

    doi:10.1002/lsm.20790

    authors: Obeidi A,McCracken MS,Liu PR,Litaker MS,Beck P,Rahemtulla F

    更新日期:2009-08-01 00:00:00

  • Tissue ablation and gas formation of two excimer laser systems: an in vitro evaluation on porcine aorta.

    abstract:BACKGROUND AND OBJECTIVE:The relationship between tissue ablation volume and the formation of insoluble gas of the currently available excimer laser systems is unknown. This aspect was evaluated in two excimer laser systems. STUDY DESIGN/MATERIALS AND METHODS:We measured tissue ablation volume and gas production of tw...

    journal_title:Lasers in surgery and medicine

    pub_type: 杂志文章

    doi:10.1002/(SICI)1096-9101(1996)18:2<197::AID-LSM11>3

    authors: Appelman YE,Piek JJ,Verhoofstad GG,Gijsbers GH,Van Gemert MJ

    更新日期:1996-01-01 00:00:00

  • Laser and suture anastomosis: passive compliance and active force production.

    abstract::One carotid from each dog underwent a laser anastomosis and the other carotid an interrupted suture repair. One or eight weeks later (n greater than or equal to 4 dogs/time period); four rings (1 mm in length) containing the laser or suture anastomosis or the normal artery (two/dog) were removed. Using a photoelectric...

    journal_title:Lasers in surgery and medicine

    pub_type: 杂志文章

    doi:10.1002/lsm.1900120213

    authors: Dalsing MC,Packer CS,Kueppers P,Griffith SL,Davis TE

    更新日期:1992-01-01 00:00:00

  • Effect of the combination of laser excision and interstitial hyperthermia in palliative therapy of head and neck tumours in the advanced stage of the disease.

    abstract:BACKGROUND AND OBJECTIVES:We evaluated the potential of combining laser excision with laser interstitial hyperthermia in order to improve the quality of life of patients with 3rd or 4th stage malignant head and neck tumours. STUDY DESIGN/MATERIALS AND METHODS:To evaluate the quality of life, we used the 4th version of...

    journal_title:Lasers in surgery and medicine

    pub_type: 杂志文章

    doi:10.1002/lsm.10253

    authors: Smucler R,Mazánek J

    更新日期:2004-01-01 00:00:00

  • Body contouring by non-invasive transdermal focused ultrasound.

    abstract:BACKGROUND AND OBJECTIVES:The risks of currently available invasive procedures in body contouring motivate a need for safer, non-invasive technologies for improving the appearance of body silhouette. A new device has been developed that uses focused therapeutic ultrasound to reduce adipose tissue non-invasively. The ai...

    journal_title:Lasers in surgery and medicine

    pub_type: 临床试验,杂志文章

    doi:10.1002/lsm.20478

    authors: Moreno-Moraga J,Valero-Altés T,Riquelme AM,Isarria-Marcosy MI,de la Torre JR

    更新日期:2007-04-01 00:00:00

  • Observations on the simultaneous use of CO2 and Nd:YAG lasers in neurosurgery.

    abstract::Seven cases of cerebral tumors (four deep-seated sovratentorial gliomas located in motor area, occipital region, parietal region and frontal region; one deep-seated cystic cerebellar spongioblastoma; one sphenoidal wing meningioma, and one spinal cord intradural tumor) were simultaneously irradiated in the same area w...

    journal_title:Lasers in surgery and medicine

    pub_type: 杂志文章

    doi:10.1002/lsm.1900020205

    authors: Fasano VA,Benech F,Ponzio RM

    更新日期:1982-01-01 00:00:00

  • Risk factors for recurrence of vaginal intraepithelial neoplasia in the vaginal vault after laser vaporization.

    abstract:BACKGROUND:We sought to investigate risk factors for recurrence of vaginal intraepithelial neoplasia (VAIN) in the vaginal vault after laser vaporization. MATERIALS AND METHODS:Among 162 patients treated with laser vaporization in the vaginal vault after hysterectomy between March 1998 and August 2007, the clinical da...

    journal_title:Lasers in surgery and medicine

    pub_type: 杂志文章

    doi:10.1002/lsm.20741

    authors: Kim HS,Park NH,Park IA,Park JH,Chung HH,Kim JW,Song YS,Kang SB

    更新日期:2009-03-01 00:00:00

  • Efficacy of early treatment of facial port wine stains in newborns: a review of 49 cases.

    abstract:BACKGROUND:Port wine stains (PWS) affect 0.3-0.5% of both sexes of newborns, usually occurring on the face. OBJECTIVE:To document safety and effectiveness of cryogen spray cooled, pulsed-dye laser at higher fluences than previously used to lighten facial PWS in infants < or =6 months, and establish that frequent treat...

    journal_title:Lasers in surgery and medicine

    pub_type: 杂志文章

    doi:10.1002/lsm.20529

    authors: Chapas AM,Eickhorst K,Geronemus RG

    更新日期:2007-08-01 00:00:00

  • Laser modulation of angiogenic factor production by T-lymphocytes.

    abstract:BACKGROUND AND OBJECTIVE:In previous investigations, small variations in the energy densities of low level light therapy (LLLT) were found to produce significant differences in the proliferation of resting T-lymphocytes in vitro. Pulsing these cells with mitogen in addition to laser therapy produced inhibitory effects ...

    journal_title:Lasers in surgery and medicine

    pub_type: 杂志文章

    doi:10.1002/(sici)1096-9101(2000)26:4<357::aid-lsm3>3.

    authors: Agaiby AD,Ghali LR,Wilson R,Dyson M

    更新日期:2000-01-01 00:00:00

  • Theramal, mechanical, optical, and morphologic changes in bovine nucleus pulposus induced by Nd:YAG (lambda = 1.32 microm) laser irradiation.

    abstract:UNLABELLED:BACKGROUND AND OBJECTIVE To examine the biophysical effects of photothermal heating on herniated intervertebral discs during laser decompression surgery. STUDY DESIGN/MATERIALS AND METHODS:Ex vivo bovine nucleus pulposus specimens were irradiated with a Nd: YAG laser (lambda = 1.32 microm, 100 seconds expos...

    journal_title:Lasers in surgery and medicine

    pub_type: 杂志文章

    doi:10.1002/lsm.1046

    authors: Choi JY,Tanenbaum BS,Milner TE,Dao XV,Nelson JS,Sobol EN,Wong BJ

    更新日期:2001-01-01 00:00:00

  • Bond strength of epoxy resin-based root canal sealer to human root dentin irradiated with Er,Cr:YSGG laser.

    abstract:OBJECTIVE:The aim was to evaluate the influence of Er,Cr:YSGG laser irradiation associated with different final irrigation protocols on the bond strength of epoxy resin-based root canal sealer to root dentin, on the dentin/filling material interface and in the temperature variation during irradiation. METHODOLOGY:Nine...

    journal_title:Lasers in surgery and medicine

    pub_type: 杂志文章

    doi:10.1002/lsm.22496

    authors: Franceschini KA,Silva-Sousa YT,Lopes FC,Pereira RD,Palma-Dibb RG,de Sousa-Neto MD

    更新日期:2016-12-01 00:00:00

  • Tooth caries detection by curve fitting of laser-induced fluorescence emission: a comparative evaluation with reflectance spectroscopy.

    abstract:BACKGROUND AND OBJECTIVES:Nitrogen laser-induced fluorescence (LIF) spectra of sound tooth consists of two broad bands centered at 440 and 490 nm, with two apparent side bands on either side. In order to locate the exact peak position of these bands and to effectively utilize the LIF spectral signatures for detection o...

    journal_title:Lasers in surgery and medicine

    pub_type: 杂志文章

    doi:10.1002/lsm.20229

    authors: Subhash N,Thomas SS,Mallia RJ,Jose M

    更新日期:2005-10-01 00:00:00

  • The effect of irrigation on peak temperatures in nerve root, dura, and intervertebral disc during laser-assisted foraminoplasty.

    abstract:BACKGROUND AND OBJECTIVE:The Holmium: YAG (Ho: YAG) laser has been used for the ablation of prolapsed discs but alternative techniques are available, and this application remains controversial. It also has potential for the decompression of nerve roots within narrowed foraminae with the technique of endoscopic laser fo...

    journal_title:Lasers in surgery and medicine

    pub_type: 杂志文章

    doi:10.1002/lsm.1083

    authors: Hafez MI,Zhou S,Coombs RR,McCarthy ID

    更新日期:2001-01-01 00:00:00

  • Characterization of tissue ablation with a continuous wave holmium laser.

    abstract:BACKGROUND AND OBJECTIVE:The pulsed holmium laser is a promising tool for tissue ablation but possesses some limitations. For example, it is capable of producing significant mechanical damage in certain tissues in the form of fissures and fractures. Because longer pulse durations should reduce mechanical damage, this s...

    journal_title:Lasers in surgery and medicine

    pub_type: 杂志文章

    doi:10.1002/(SICI)1096-9101(1996)19:1<97::AID-LSM11>3.

    authors: Domankevitz Y,McMillan K,Nishioka NS

    更新日期:1996-01-01 00:00:00

  • Modeling and simulations of the pharmacokinetics of fluorophore conjugated antibodies in tumor vicinity for the optimization of fluorescence-based optical imaging.

    abstract:BACKGROUND AND OBJECTIVES:One of the methods to detect and localize tumors in tissue is to use fluorophore conjugated specific antibodies as tumor surface markers. The goals of this study are to understand and quantify the pharmacokinetics of fluorophore conjugated antibodies in the vicinity of a tumor. This study conc...

    journal_title:Lasers in surgery and medicine

    pub_type: 杂志文章

    doi:10.1002/lsm.20200

    authors: Fibich G,Hammer A,Gannot G,Gandjbakhche A,Gannot I

    更新日期:2005-08-01 00:00:00

  • Low power laser irradiation alters the rate of regeneration of the rat facial nerve.

    abstract::Low power laser irradiation has been reported to cause biological effects due to the photochemical and/or photophysical action of the radiation. This study determined quantitatively if transcutaneous low power laser irradiation can affect the regeneration of the rat facial nerve. The facial nerve was crushed unilatera...

    journal_title:Lasers in surgery and medicine

    pub_type: 杂志文章

    doi:10.1002/lsm.1900130113

    authors: Anders JJ,Borke RC,Woolery SK,Van de Merwe WP

    更新日期:1993-01-01 00:00:00

  • Treatment of vitiligo by 308-nm excimer laser: an evaluation of variables affecting treatment response.

    abstract:BACKGROUND AND OBJECTIVES:To determine the true efficacy of the 308-nm excimer laser for the treatment of vitiligo, while taking into account confounding factors such as anatomic site of treatment, age, sex, skin type, MED, and duration of evolution of the vitiligo. STUDY DESIGN/MATERIALS AND METHODS:Thirty-five patie...

    journal_title:Lasers in surgery and medicine

    pub_type: 临床试验,杂志文章

    doi:10.1002/lsm.20057

    authors: Ostovari N,Passeron T,Zakaria W,Fontas E,Larouy JC,Blot JF,Lacour JP,Ortonne JP

    更新日期:2004-01-01 00:00:00

  • A Comparison of Human and Porcine Skin in Laser-Assisted Drug Delivery of Chemotherapeutics.

    abstract:BACKGROUND AND OBJECTIVES:Porcine skin is a widely used model in diffusion studies, but its usefulness for laser-assisted drug delivery (LADD) has not been evaluated in comparison with human skin. This study compared porcine and human skin in ex vivo LADD diffusion studies. STUDY DESIGN/MATERIALS AND METHODS:Ex vivo a...

    journal_title:Lasers in surgery and medicine

    pub_type:

    doi:10.1002/lsm.23344

    authors: Rosenberg LK,Bagger C,Janfelt C,Haedersdal M,Olesen UH,Lerche CM

    更新日期:2020-11-08 00:00:00

  • Treatment of leg veins with the long pulse dye laser using variable pulse durations and energy fluences.

    abstract:BACKGROUND AND OBJECTIVES:Attempts at using the pulsed dye laser (PDL) operating at 585 nm wavelength and 0.45 milliseconds pulse duration to treat leg veins have been notably unsuccessful. The aim of this study is to evaluate the efficacy and complications of the 595 nm long PDL in the treatment of leg veins. STUDY D...

    journal_title:Lasers in surgery and medicine

    pub_type: 杂志文章

    doi:10.1002/lsm.20035

    authors: Kono T,Yamaki T,Erçöçen AR,Fujiwara O,Nozaki M

    更新日期:2004-01-01 00:00:00

  • Fine structure of zonal changes in experimental Nd:YAG laser-induced interstitial hyperthermia.

    abstract::Interstitial thermotherapy using Nd:YAG-laser induced hyperthermia is a new stereotactic method for the treatment of brain tumors in poorly accessible regions. To provide a basis for the underlying tissue alterations, we have analyzed the spatial and temporal pattern of interstitial laser hyperthermia lesions in the n...

    journal_title:Lasers in surgery and medicine

    pub_type: 杂志文章

    doi:10.1002/lsm.1900130212

    authors: Schober R,Bettag M,Sabel M,Ulrich F,Hessel S

    更新日期:1993-01-01 00:00:00

  • Prospective study of port wine stain treatment with dye laser: comparison of two wavelengths (585 nm vs. 595 nm) and two pulse durations (0.5 milliseconds vs. 20 milliseconds).

    abstract:BACKGROUND AND OBJECTIVES:The conventional pulsed-dye laser (wavelength 585 nm, pulse duration 0.5 milliseconds) is seen as the standard treatment for port wine stains (PWS). Using the pulsed-dye laser at wavelengths of 590, 595, and 600 nm and at varying pulse durations of 1.5-40 milliseconds is one of the newest deve...

    journal_title:Lasers in surgery and medicine

    pub_type: 临床试验,杂志文章,随机对照试验

    doi:10.1002/lsm.20003

    authors: Greve B,Raulin C

    更新日期:2004-01-01 00:00:00

  • 5-ALA in the management of malignant glioma.

    abstract:BACKGROUND:Patients suffering from malignant gliomas have a poor prognosis. For the surgical treatment of these tumors, 5-aminolevulinic acid (5-ALA) has become a new standard. AIMS:This review intends to provide an overview over current status, significance, limitations, and future perspectives of 5-ALA based fluores...

    journal_title:Lasers in surgery and medicine

    pub_type: 杂志文章,评审

    doi:10.1002/lsm.22933

    authors: Stepp H,Stummer W

    更新日期:2018-07-01 00:00:00

  • Effect of helium-neon and infrared laser irradiation on wound healing in rabbits.

    abstract::We examined the biostimulating effects of helium-neon laser radiation (HeNe; 632.8 nm), pulsed infrared laser radiation (IR; 904 nm), and the two combined on skin wound healing in New Zealand white rabbits. Seventy-two rabbits received either 1) no exposure, 2) 1.65 J/cm2 HeNe, 3) 8.25 J/cm2 pulsed IR, or 4) both HeNe...

    journal_title:Lasers in surgery and medicine

    pub_type: 杂志文章

    doi:10.1002/lsm.1900090111

    authors: Braverman B,McCarthy RJ,Ivankovich AD,Forde DE,Overfield M,Bapna MS

    更新日期:1989-01-01 00:00:00

  • An expanded study of long-pulsed 1064 nm Nd:YAG laser treatment of basal cell carcinoma.

    abstract:BACKGROUND AND OBJECTIVE:Basal cell carcinoma (BCC) is an indolent form of skin cancer that is rarely life threatening, but can cause significant cosmetic and functional morbidity. Surgical treatments often result in disfiguring scars, while topical therapies frequently result in recurrence. The need for a more effecti...

    journal_title:Lasers in surgery and medicine

    pub_type: 杂志文章

    doi:10.1002/lsm.22803

    authors: Ortiz AE,Anderson RR,DiGiorgio C,Jiang SIB,Shafiq F,Avram MM

    更新日期:2018-02-13 00:00:00

  • Free electron laser infrared wavelength specificity for cutaneous contraction.

    abstract:BACKGROUND AND OBJECTIVE:Short pulsed and scanned CO(2) lasers that target water molecules are currently used for cutaneous resurfacing. These CO(2) resurfacing lasers produce acute cutaneous contraction, which can be quantitated as a measure of the laser's effect. We postulated that targeting the vibrational and rotat...

    journal_title:Lasers in surgery and medicine

    pub_type: 杂志文章

    doi:10.1002/(sici)1096-9101(1999)25:1<1::aid-lsm1>3.0.

    authors: Ellis DL,Weisberg NK,Chen JS,Stricklin GP,Reinisch L

    更新日期:1999-01-01 00:00:00

  • Measurement of thermal effects on the optical properties of prostate tissue at wavelengths of 1,064 and 633 nm.

    abstract:BACKGROUND AND OBJECTIVE:The extent of thermal injury during laser prostatectomy is dependent on the light distribution in laser-irradiated tissue. As tissue is irradiated, the optical properties change as a function of temperature due to an alteration of molecular and cellular structure. The purpose of the present stu...

    journal_title:Lasers in surgery and medicine

    pub_type: 杂志文章

    doi:10.1002/(sici)1096-9101(1999)24:1<38::aid-lsm7>3.0

    authors: Nau WH,Roselli RJ,Milam DF

    更新日期:1999-01-01 00:00:00

  • Histological studies of myocardium zones irradiated with Nd-YAG laser.

    abstract::Histological investigation of the dog's myocardium performed 7 and 25 days after irradiation with different doses of Nd-YAG laser show that the damage zone has the shape of a hollow sphere with minor changes in cardiomyocytes in the central part of the sphere. The small blood vessels in the irradiated zone remained un...

    journal_title:Lasers in surgery and medicine

    pub_type: 杂志文章

    doi:10.1002/lsm.1900050506

    authors: Obelienius V,Knepa A,Lubite Y,Ambartzumian R,Isakov V,Koshelev E,Markin E

    更新日期:1985-01-01 00:00:00