Abstract:
BACKGROUND AND OBJECTIVES:To investigate the effect of photodynamic therapy (PDT) on cell death in malignant tumor tissue, the frequency of terminal deoxynucleotidyl transferase-mediated dUTP nick end-labeling (TUNEL)-positive cells and the possible involvement of Fas and Fas ligand system were evaluated. STUDY DESIGN/MATERIALS AND METHODS:NR-S1 tumor-bearing C3H/HeNCrj mice were treated by PDT with Photofrin(R) (12 mg/kg body weight) and Nd:YAG dye laser (630 nm, 10 Hz, 150 J/cm(2)). Paraffin-embedded tissue sections from the excised tumor tissues at 6, 12, 24, 48 hours after PDT were analyzed by TUNEL for the occurrence of apoptosis and by immunohistochemistry for Fas and Fas ligand (FasL) expression. TUNEL-positive cells as well as Fas- or FasL-positive cells were counted and expressed as a percentage of positive cells per total cells. RESULTS:Based on the percent area of tumor necrotic foci, the most effective conditions for PDT were first determined. Under these conditions, PDT increased the number of TUNEL-positive tumor cells at 12 hours after irradiation. In parallel with the increase in TUNEL-positive cells, Fas-positive tumor cells were also found in the same area where many TUNEL-positive tumor cells were found. The expression of Fas ligand was found in the tumor cells surrounding TUNEL-positive cells on serial sections. A significant increase in FasL-positive lymphocytes was observed at 12 hours, whereas the infiltration of such lymphocytes into the area where TUNEL-positive tumor cells were observed was rare. CONCLUSION:The possible role of Fas/FasL system in the cell death induced by PDT with Photofrin(R) and Nd:YAG dye laser was suggested. Moreover, the role of infiltrated lymphocytes seemed not to be so much in this model.
journal_name
Lasers Surg Medjournal_title
Lasers in surgery and medicineauthors
Yokota T,Ikeda H,Inokuchi T,Sano K,Koji Tdoi
10.1002/1096-9101(2000)26:5<449::aid-lsm4>3.0.co;2subject
Has Abstractpub_date
2000-01-01 00:00:00pages
449-60issue
5eissn
0196-8092issn
1096-9101pii
10.1002/1096-9101(2000)26:5<449::AID-LSM4>3.0.CO;2journal_volume
26pub_type
杂志文章abstract:BACKGROUND AND OBJECTIVE:To investigate silicone naphthalocyanine (SINc; 0.5 mg/kg) for photodynamic therapy (PDT) of the ciliary body in pigmented rabbits. STUDY DESIGN/MATERIALS AND METHODS:SINc was dissolved in canola oil by heating, emulsified with Tween-80, and given by ear vein. Pharmacokinetics were studied in ...
journal_title:Lasers in surgery and medicine
pub_type: 杂志文章
doi:10.1002/(SICI)1096-9101(1996)18:1<86::AID-LSM11>3.
更新日期:1996-01-01 00:00:00
abstract:BACKGROUND AND OBJECTIVES:The aim of the in vitro study was to examine the clinical efficacy of semiconductor laser periodontal pocket irradiation as an adjunct to conventional scaling and root planing. MATERIALS AND METHODS:Twenty-two healthy patients with a need of periodontal treatment (15 women, 7 men, mean age 45...
journal_title:Lasers in surgery and medicine
pub_type: 杂志文章
doi:10.1002/lsm.20252
更新日期:2005-12-01 00:00:00
abstract:OBJECTIVES:Intravascular photoacoustic (IVPA) imaging is being developed to image atherosclerotic plaques, a leading cause of morbidity and mortality in the United States. However, the safety of this imaging modality, which requires repeated irradiation with short laser pulses, has not yet been investigated. This study...
journal_title:Lasers in surgery and medicine
pub_type: 杂志文章
doi:10.1002/lsm.23026
更新日期:2018-10-10 00:00:00
abstract:BACKGROUND AND OBJECTIVE:The metabolic flexibility is often impaired in diseases associated with obesity, and many studies are based on the hypothesis that dysfunction in peripheral tissues such as skeletal muscle, liver and adipose tissue represent the etiology of development of metabolic inflexibility. Experimental e...
journal_title:Lasers in surgery and medicine
pub_type: 杂志文章,随机对照试验
doi:10.1002/lsm.22395
更新日期:2015-10-01 00:00:00
abstract:BACKGROUND AND OBJECTIVES:This study was conducted to clarify the morphologic characteristics and subsequent repair process of coagulation necrosis produced by pulsed CO(2) laser irradiation with relatively low fluence, and thereby to evaluate the clinical efficacy of this irradiation mode. STUDY DESIGN/MATERIALS AND ...
journal_title:Lasers in surgery and medicine
pub_type: 杂志文章
doi:10.1002/lsm.20712
更新日期:2008-12-01 00:00:00
abstract:BACKGROUND:Pulsed dye laser (PDL) is the first choice for treatment of port wine stains (PWS). However, outcome is highly variable and only a few patients achieve complete clearance. The objective of the study was to compare efficacy and safety of single pass PDL with double pass PDL at a 6 minute interval. METHODS:We...
journal_title:Lasers in surgery and medicine
pub_type: 杂志文章,随机对照试验
doi:10.1002/lsm.22011
更新日期:2012-03-01 00:00:00
abstract::The feasibility of using a pulsed dye laser in angioplasty for detection and disintegration of calcified plaques was studied in vitro. A flashlamp-pumped dye laser (495 nm, 2 microseconds) was used as the exciting source for laser-induced-fluorescence (LIF) signals. Spectral data in the 520 nm to 800 nm region of norm...
journal_title:Lasers in surgery and medicine
pub_type: 杂志文章
doi:10.1002/lsm.1900110207
更新日期:1991-01-01 00:00:00
abstract::To date, there are two fairly well-established alternatives for laser-induced shock-wave lithotripsy in clinical practice. The Q-switched Nd:YAG laser is distinguished by the high-stone selectivity of its coupler systems. The necessity of a coupler system and its fairly small conversion rate of light energy into mecha...
journal_title:Lasers in surgery and medicine
pub_type: 杂志文章
doi:10.1002/lsm.1900080405
更新日期:1988-01-01 00:00:00
abstract:BACKGROUND AND OBJECTIVES:Attempts at using the pulsed dye laser (PDL) operating at 585 nm wavelength and 0.45 milliseconds pulse duration to treat leg veins have been notably unsuccessful. The aim of this study is to evaluate the efficacy and complications of the 595 nm long PDL in the treatment of leg veins. STUDY D...
journal_title:Lasers in surgery and medicine
pub_type: 杂志文章
doi:10.1002/lsm.20035
更新日期:2004-01-01 00:00:00
abstract:BACKGROUND AND OBJECTIVES:During transmyocardial laser revascularization (TMLR), multiple microembolic signals (MES) can be detected in cerebral arteries. We sought to characterize composition and clinical relevance of these MES and to evaluate strategies to reduce cerebral microembolization during TMLR. STUDY DESIGN/...
journal_title:Lasers in surgery and medicine
pub_type: 杂志文章
doi:10.1002/lsm.20030
更新日期:2004-01-01 00:00:00
abstract:BACKGROUND AND OBJECTIVE:Basal cell carcinoma (BCC) is an indolent form of skin cancer that is rarely life threatening, but can cause significant cosmetic and functional morbidity. Surgical treatments often result in disfiguring scars, while topical therapies frequently result in recurrence. The need for a more effecti...
journal_title:Lasers in surgery and medicine
pub_type: 杂志文章
doi:10.1002/lsm.22803
更新日期:2018-02-13 00:00:00
abstract:BACKGROUND AND OBJECTIVES:Whether light emitting diode (LED) irradiation has effects on "beta-amyloid (A beta) induced apoptosis," a leading hypothesis of the cause of Alzheimer's disease (AD), or not? STUDY DESIGN/MATERIALS AND METHODS:Monolayer cell cultures of PC12 were subjected to A beta or/and LED irradiation at...
journal_title:Lasers in surgery and medicine
pub_type: 杂志文章
doi:10.1002/lsm.10216
更新日期:2003-01-01 00:00:00
abstract:BACKGROUND AND OBJECTIVE:Progressive ascending telangiectasia (PAT) is a distinct entity with telangiectatic superficial vessels on lower extremities as its main clinical feature. A relationship with occult infections and response to antibiotic and antifungal drugs have been described, although not all cases can be suc...
journal_title:Lasers in surgery and medicine
pub_type: 杂志文章
doi:10.1002/(sici)1096-9101(1997)21:5<413::aid-lsm1>3.
更新日期:1997-01-01 00:00:00
abstract:BACKGROUND AND OBJECTIVES:Kenalog application onto surgical wounds has been used to control dynamic pain following Laser-Assisted-Uvulo-Palatoplasty (LAUP) with good results in our department. When the effect was sub-optimal, insufficient ointment over the superior-lateral corner of wounds were always noted. Fulfilling...
journal_title:Lasers in surgery and medicine
pub_type: 杂志文章
doi:10.1002/lsm.21122
更新日期:2011-11-01 00:00:00
abstract:BACKGROUND AND OBJECTIVE:The reactive oxygen mechanisms associated with cell damage after photodynamic therapy (PDT) may be exploited to enhance tumor destruction. Pharmacological reduction of glutathione (GSH), an inhibitor of reactive oxygen species, can be induced by administration of buthionine sulfoximine (BSO). ...
journal_title:Lasers in surgery and medicine
pub_type: 杂志文章
doi:10.1002/(sici)1096-9101(1998)23:3<161::aid-lsm5>3.
更新日期:1998-01-01 00:00:00
abstract:BACKGROUND AND OBJECTIVE:Varied physical and chemical characteristic of root canal sealers and different irrigant agitation systems can influence the depth of penetration. The aim of this in vitro study was to use a laser scanning confocal microscope in order to assess the dentinal tubules penetration of various sealer...
journal_title:Lasers in surgery and medicine
pub_type: 杂志文章
doi:10.1002/lsm.22446
更新日期:2016-01-01 00:00:00
abstract:BACKGROUND AND OBJECTIVES:The risks of currently available invasive procedures in body contouring motivate a need for safer, non-invasive technologies for improving the appearance of body silhouette. A new device has been developed that uses focused therapeutic ultrasound to reduce adipose tissue non-invasively. The ai...
journal_title:Lasers in surgery and medicine
pub_type: 临床试验,杂志文章
doi:10.1002/lsm.20478
更新日期:2007-04-01 00:00:00
abstract:BACKGROUND AND OBJECTIVES:Inhibition of cyclooxygenase (COX) and prostaglandin E(2) (PGE(2)) protects cells against cell injury in specific pathophysiological situations: inflammation and oxidative stress. Although the anti-inflammatory effects have been reported in clinical fields for specific wavelength irradiation d...
journal_title:Lasers in surgery and medicine
pub_type: 杂志文章
doi:10.1002/lsm.20533
更新日期:2007-08-01 00:00:00
abstract::Using an optically shielded fiber optic laser catheter, the amount of gas produced when firing an argon ion laser into 0.9% saline solution or blood alone and into atheromatous aorta in either a blood or 0.9% saline medium was quantitated. Energies from 0.25 to 4 joules (J) were used at powers of 2, 5, and 8 W. We fou...
journal_title:Lasers in surgery and medicine
pub_type: 杂志文章
doi:10.1002/lsm.1900080113
更新日期:1988-01-01 00:00:00
abstract:BACKGROUND:Port wine stains (PWS) affect 0.3-0.5% of both sexes of newborns, usually occurring on the face. OBJECTIVE:To document safety and effectiveness of cryogen spray cooled, pulsed-dye laser at higher fluences than previously used to lighten facial PWS in infants < or =6 months, and establish that frequent treat...
journal_title:Lasers in surgery and medicine
pub_type: 杂志文章
doi:10.1002/lsm.20529
更新日期:2007-08-01 00:00:00
abstract:OBJECTIVES:Cryolipolysis of the arms has been demonstrated to be an effective treatment for non-invasive reduction of subcutaneous fat. This study evaluated the safety and efficacy of the concurrent use of a new commercially-available small applicator in conjunction with an existing medium sized applicator for the cust...
journal_title:Lasers in surgery and medicine
pub_type: 杂志文章
doi:10.1002/lsm.22811
更新日期:2018-03-22 00:00:00
abstract::Photodynamic therapy (PDT) is a promising approach to the treatment of cancer. Preferential retention of the photosensitizer by malignant tissue has been considered a hallmark of this treatment modality. However, photosensitivity can be observed in normal, non-neoplastic tissues, and the present study investigated the...
journal_title:Lasers in surgery and medicine
pub_type: 杂志文章
doi:10.1002/lsm.1900110110
更新日期:1991-01-01 00:00:00
abstract::Previous reports of effects of CO2 laser energy on human fibrocartilage suggest thermal injury extends to a depth of approximately 70 microns from the target surface with power settings of 35 W and exposure times of 0.5 seconds. The present study was undertaken to look for more subtle evidence of thermal alteration of...
journal_title:Lasers in surgery and medicine
pub_type: 杂志文章
doi:10.1002/lsm.1900070209
更新日期:1987-01-01 00:00:00
abstract:BACKGROUND AND OBJECTIVE:Moraxella catarrhalis is a significant cause of pediatric otitis media (OM), which is the most prevalent bacterial infection in children and primary reason for antibiotic administration in this population. Moreover, biofilm formation has been implicated as a primary mechanism of chronic or recu...
journal_title:Lasers in surgery and medicine
pub_type: 杂志文章
doi:10.1002/lsm.22287
更新日期:2014-11-01 00:00:00
abstract:BACKGROUND AND OBJECTIVES:Sutures are currently the gold standard for wound closure but they are still unable to seal tissue and may induce scarring or inflammation. Biocompatible glues, based on polysaccharides such as chitosan, are a possible alternative to conventional wound closure. In this study, the adhesion of l...
journal_title:Lasers in surgery and medicine
pub_type: 杂志文章
doi:10.1002/lsm.20418
更新日期:2007-01-01 00:00:00
abstract:OBJECTIVE:Dental composite restorative materials are color matched to the tooth and are difficult to remove by mechanical means without excessive removal or damage to peripheral enamel and dentin. Lasers are ideally suited for selective ablation to minimize healthy tissue loss when replacing existing restorations, seal...
journal_title:Lasers in surgery and medicine
pub_type: 杂志文章
doi:10.1002/lsm.21111
更新日期:2011-09-01 00:00:00
abstract:BACKGROUND AND OBJECTIVE:The purpose of this study was to compare the effect of Low-Level Laser Therapy (LLLT) with sham and whirlpool treatment on the contracted knee joint in rat. STUDY DESIGN/MATERIALS AND METHODS:Forty-eight Wistar rats were operated on to immobilize knee joint, and 1 week after operation they wer...
journal_title:Lasers in surgery and medicine
pub_type: 杂志文章
doi:10.1002/(sici)1096-9101(1998)22:2<81::aid-lsm3>3.0
更新日期:1998-01-01 00:00:00
abstract::For laser angioplasty probes, the thermal properties of the probes will primarily determine their mechanism of action. We examined the absorption, temperature increase, and probe degradation of transparent contact probes (hemispherical contact probe and ball-shaped fibers) and metal laser probes coupled to a continuou...
journal_title:Lasers in surgery and medicine
pub_type: 杂志文章
doi:10.1002/lsm.1900110304
更新日期:1991-01-01 00:00:00
abstract:OBJECTIVE:The aim was to evaluate the influence of Er,Cr:YSGG laser irradiation associated with different final irrigation protocols on the bond strength of epoxy resin-based root canal sealer to root dentin, on the dentin/filling material interface and in the temperature variation during irradiation. METHODOLOGY:Nine...
journal_title:Lasers in surgery and medicine
pub_type: 杂志文章
doi:10.1002/lsm.22496
更新日期:2016-12-01 00:00:00
abstract:OBJECTIVES:Photodynamic therapy (PDT) is an effective cancer treatment that uses photosensitizers, light, and oxygen to destroy malignant cells. Porphyrins, and in particular the cationic derivatives, are the most investigated photosensitizers for PDT. In this context, it is important to study new methodologies to deve...
journal_title:Lasers in surgery and medicine
pub_type: 杂志文章
doi:10.1002/lsm.22824
更新日期:2018-07-01 00:00:00