Abstract:
OBJECTIVES:Intravascular photoacoustic (IVPA) imaging is being developed to image atherosclerotic plaques, a leading cause of morbidity and mortality in the United States. However, the safety of this imaging modality, which requires repeated irradiation with short laser pulses, has not yet been investigated. This study has two objectives. First, determine in vitro the limit of cumulative fluence that can be applied to cells before death at IVPA relevant wavelengths. Second, evaluate if high single pulse fluences are a potential cause of cell death during IVPA imaging. MATERIALS AND METHODS:Experiments were conducted using endothelial cells, macrophages, and smooth muscle cells. The cumulative fluence experiments were conducted at 1064 and 1197 nm, using a high pulse repetition frequency laser. Cells were irradiated with a wide range of cumulative fluences and evaluated for cell death. The thresholds for death were compared to the maximum expected clinical cumulative fluence. To evaluate the effect of single pulse fluences, cells were irradiated at 1064, 1210, and 1720 nm. Light was delivered at a range of pulse energies to emulate the fluences that cells would be exposed to during clinical IVPA imaging. RESULTS:At 1064 nm, all three cell types remained viable at cumulative fluences above the maximum expected clinical cumulative fluence, which is calculated based on common IVPA imaging protocols. At 1197 nm, cells were viable near or just below the maximum expected clinical cumulative fluence, with some cell type to cell type variation. All three cell types remained viable after irradiation with high single pulse fluences at all three wavelengths. CONCLUSION:The cumulative fluence experiments indicate that safety considerations are likely to put constraints on the amount of irradiation that can be used in IVPA imaging protocols. However, this study also indicates that it will be possible to use IVPA imaging safely, since cumulative fluences could be reduced by as much as two orders of magnitude below the maximum expected clinical cumulative fluence by varying the imaging protocol, albeit at the expense of image quality. The single pulse fluence experiments indicate that cell death from single pulse fluence is not likely during IVPA imaging. Thus, future studies should focus on heat accumulation as the likely mechanism of tissue damage. Lasers Surg. Med. © 2018 Wiley Periodicals, Inc.
journal_name
Lasers Surg Medjournal_title
Lasers in surgery and medicineauthors
Sowers T,VanderLaan D,Karpiouk A,Donnelly EM,Smith E,Emelianov Sdoi
10.1002/lsm.23026subject
Has Abstractpub_date
2018-10-10 00:00:00eissn
0196-8092issn
1096-9101pub_type
杂志文章abstract:BACKGROUND AND OBJECTIVES:Facial teleangectasias can be a relevant cosmetic problem, which the patient usually hides with a thick layer of makeup. This study will describe the response on these vascular lesions using the intense pulsed light (IPL) source. METHODS:Five hundred eighteen consecutive patients were treated...
journal_title:Lasers in surgery and medicine
pub_type: 杂志文章
doi:10.1002/lsm.20187
更新日期:2005-07-01 00:00:00
abstract:OBJECTIVES:Our skin is constantly exposed to light from solar radiation and electronic devices, which impact skin physiology and aging. The biological altering properties of ultraviolet (UV) solar radiation on skin have been well established. There is significant scientific and public interest on the effects of electro...
journal_title:Lasers in surgery and medicine
pub_type: 杂志文章
doi:10.1002/lsm.22794
更新日期:2018-02-05 00:00:00
abstract:BACKGROUND AND OBJECTIVES:For Asian skin, recent "non-ablative" skin rejuvenation techniques have become the focus of attention for darker complected patients. In our earlier research, we have shown that intense pulsed light (IPL) technology can be applied to Asian skin with a high degree of safety and efficacy. In thi...
journal_title:Lasers in surgery and medicine
pub_type: 杂志文章
doi:10.1002/lsm.10036
更新日期:2002-01-01 00:00:00
abstract:BACKGROUND AND OBJECTIVES:Variable square pulse (VSP) Er:YAG laser should be quicker than older Er:YAG lasers. The objectives were: (1) comparison of VSP laser and mechanical handpiece efficacy for apicectomy and (2) determination of optimal pulse width/energy/frequency combination. STUDY DESIGN/MATERIALS AND METHODS:...
journal_title:Lasers in surgery and medicine
pub_type: 杂志文章
doi:10.1002/lsm.20168
更新日期:2005-06-01 00:00:00
abstract:BACKGROUND AND OBJECTIVES:CO2 laser cordectomy has been the workhorse of laser surgery for early glottic squamous cell carcinoma (GSCC) since the early 1970s. During the last decades, potassium titanyl phosphate (KTP) laser surgery for early GSCC gained popularity, introducing the tumor ablation technique. Yet, there a...
journal_title:Lasers in surgery and medicine
pub_type:
doi:10.1002/lsm.23202
更新日期:2020-09-01 00:00:00
abstract:BACKGROUND AND OBJECTIVES:The sanitation of the root canal system and the adjacent dentin has always been a key requirement for successful endodontics. In recent years, various laser systems have provided a major contribution to this aim, namely the Nd:YAG-, the 810 nm Diode-, the Er:YAG-, and the Er,Cr:YSGG laser. Num...
journal_title:Lasers in surgery and medicine
pub_type: 杂志文章
doi:10.1002/lsm.20331
更新日期:2006-07-01 00:00:00
abstract:BACKGROUND AND OBJECTIVES:Chronic sun-exposure results in five main changes to exposed skin: telangiectasia, hyperpigmentation, rhytides, enlarged pores, and sagging skin. Various laser and non-laser modalities have been used to improve sun-damaged skin. The pulsed-dye laser (PDL) has an unparalleled safety record and ...
journal_title:Lasers in surgery and medicine
pub_type: 杂志文章
doi:10.1002/lsm.20463
更新日期:2007-02-01 00:00:00
abstract:BACKGROUND AND OBJECTIVE:We reported the use of Holmium-YAG laser in the management of a foreign body impacted in the esophagus. STUDY DESIGN/PATIENTS AND METHODS:Esophagoscopy was performed on a man with a denture impacted in the esophagus. The denture was disimpacted into the stomach and fractured with Holmium-YAG l...
journal_title:Lasers in surgery and medicine
pub_type: 杂志文章
doi:10.1002/(sici)1096-9101(1997)20:4<480::aid-lsm16>3
更新日期:1997-01-01 00:00:00
abstract::Since most techniques of refractive surgery currently in use or being developed have the potential for significant side effects, there is a need for investigating alternative procedures. We herein report on the use of a pulsed CO2 laser beam delivered through a pair of complementary axicons to produce a ring of stroma...
journal_title:Lasers in surgery and medicine
pub_type: 杂志文章
doi:10.1002/lsm.1900120305
更新日期:1992-01-01 00:00:00
abstract::Progress in the young field of laser recanalization of occluded arteries and applications in cardiovascular disease to which many investigators have contributed is briefly summarized. Specific problems remaining to be solved are set forth. In conclusion, the author makes a cautious prediction of success for the percut...
journal_title:Lasers in surgery and medicine
pub_type: 杂志文章
doi:10.1002/lsm.1900060412
更新日期:1986-01-01 00:00:00
abstract:OBJECTIVE:Inflammation is a well-known consequence of surgery. Although surgical debulking of tumor is beneficial to patients, the onset of inflammation in injured tissue may impede the success of adjuvant therapies. One marker for postoperative inflammation is IL-6, which is released as a consequence of surgical injur...
journal_title:Lasers in surgery and medicine
pub_type: 杂志文章,随机对照试验
doi:10.1002/lsm.22934
更新日期:2018-07-01 00:00:00
abstract:BACKGROUND AND OBJECTIVE:The aim of this study was to evaluate the efficacy and safety of the intense pulsed light source (IPL) and the Nd:YAG laser in the treatment of facial rhytids. Both systems can be used in a non-ablative manner and cause a dermal wound. This is thought to stimulate the production of new collagen...
journal_title:Lasers in surgery and medicine
pub_type: 临床试验,杂志文章
doi:10.1002/lsm.1029
更新日期:2001-01-01 00:00:00
abstract:BACKGROUND AND OBJECTIVES:There are some indications that low-level laser therapy (LLLT) may delay the development of skeletal muscle fatigue during high-intensity exercise. There have also been claims that LED cluster probes may be effective for this application however there are differences between LED and laser sour...
journal_title:Lasers in surgery and medicine
pub_type: 杂志文章,随机对照试验
doi:10.1002/lsm.20810
更新日期:2009-10-01 00:00:00
abstract:BACKGROUND AND OBJECTIVE:Laryngeal lesions are usually investigated by microlaryngoscopy, biopsy, and histopathology. This study aimed to evaluate the combined use of Narrow Band Imaging (NBI) and High-Speed Imaging (HSI) in the differentiation of glottic lesions in awake patients. STUDY DESIGN:Prospective diagnostic ...
journal_title:Lasers in surgery and medicine
pub_type: 临床试验,杂志文章
doi:10.1002/lsm.22652
更新日期:2017-08-01 00:00:00
abstract::Laser-induced fluorescence may be used to guide laser ablation of atherosclerotic lesions. This study was performed to evaluate arterial autofluorescence spectroscopy in vitro using a single XeCl excimer laser (308 nm) for simultaneous tissue ablation and fluorescence excitation. The laser beam was coupled to a 600-mi...
journal_title:Lasers in surgery and medicine
pub_type: 杂志文章
doi:10.1002/lsm.1900140306
更新日期:1994-01-01 00:00:00
abstract::Fluorescence spectroscopy is a promising new technique for discrimination of normal and atherosclerotic arterial tissues. It has been suggested that this technique be used as a guidance system for laser angiosurgery catheters; however, irradiation by 476-nm light can change the spectroscopic properties of arterial tis...
journal_title:Lasers in surgery and medicine
pub_type: 杂志文章
doi:10.1002/lsm.1900090606
更新日期:1989-01-01 00:00:00
abstract:BACKGROUND AND OBJECTIVES:Port-wine stain (PWS) is a congenital, progressive vascular malformation of the dermis. The use of optical coherence tomography (OCT) for the characterization of blood vessels in PWS skin has been demonstrated by several groups. In the past few years, advances in OCT technology have greatly in...
journal_title:Lasers in surgery and medicine
pub_type: 杂志文章
doi:10.1002/lsm.22194
更新日期:2013-12-01 00:00:00
abstract:BACKGROUND AND OBJECTIVES:Er:YAG laser has been used for caries removal and cavity preparation, using ablative parameters. Its effect on the margins of restorations submitted to cariogenic challenge has not yet been sufficiently investigated. The aim of this study was to assess the enamel adjacent to restored Er:YAG la...
journal_title:Lasers in surgery and medicine
pub_type: 杂志文章,随机对照试验
doi:10.1002/lsm.20684
更新日期:2008-11-01 00:00:00
abstract:BACKGROUND AND OBJECTIVE:Linear birefringence is an anisotropic property of rat tail tendon, which is largely composed of collagen. Our goal is to show that the dynamic range and sensitivity of the linear birefringence loss of collagen during heating are sufficient for kinetic modeling of the reaction. STUDY DESIGN, M...
journal_title:Lasers in surgery and medicine
pub_type: 杂志文章
doi:10.1002/(sici)1096-9101(1997)20:3<310::aid-lsm10>3
更新日期:1997-01-01 00:00:00
abstract:BACKGROUND AND OBJECTIVES:Intense pulsed light (IPL) is regarded as the gold standard of nonablative photo-rejuvenation. Yet there is still a need to observe its efficacy and safety on dark skin using a split-face module. STUDY DESIGN/MATERIALS AND METHODS:Twenty-four Chinese women with photoaging were enrolled in thi...
journal_title:Lasers in surgery and medicine
pub_type: 杂志文章,随机对照试验
doi:10.1002/lsm.20889
更新日期:2010-02-01 00:00:00
abstract:OBJECTIVE:The aim of this study was to evaluate the effect of relatively novel approach of application of polychromatic light waves on flap survival of experimental musculocutaneous flap model and to investigate efficacy of this modality as a delay procedure to increase vascularization of zone 4 of transverse rectus ab...
journal_title:Lasers in surgery and medicine
pub_type: 杂志文章
doi:10.1002/lsm.23063
更新日期:2019-08-01 00:00:00
abstract::We have determined exposure conditions for minimal damage to the corneal epithelium in rabbit using a continuous wave Tm: YAG laser operating in the TEM00 mode at incident powers between 80 and 450 mW. The 1/e beam diameter was 0.94 mm and exposure durations for threshold damage ranged from 4.3 to 0.08 sec. Calculated...
journal_title:Lasers in surgery and medicine
pub_type: 杂志文章
doi:10.1002/lsm.1900120605
更新日期:1992-01-01 00:00:00
abstract:BACKGROUND AND OBJECTIVE:Very few treatments for striae are based on prospective randomized trials. The objective of this study was to assess the efficacy of bipolar fractional radiofrequency and bipolar radiofrequency potentiated with infrared light, alone or combined, for treating abdominal stretch marks. STUDY DESI...
journal_title:Lasers in surgery and medicine
pub_type: 杂志文章,多中心研究,随机对照试验
doi:10.1002/lsm.22458
更新日期:2016-03-01 00:00:00
abstract:BACKGROUND AND OBJECTIVE:Low-intensity laser irradiation is claimed to enhance wound healing. Healing after myocardial infarction results in ventricular enlargement and wall thinning. If laser treatment accelerated cardiac healing, we speculated that ventricular remodeling would be attenuated. STUDY DESIGN/MATERIALS A...
journal_title:Lasers in surgery and medicine
pub_type: 杂志文章
doi:10.1002/1096-9101(2000)27:1<29::aid-lsm4>3.0.co;2-
更新日期:2000-01-01 00:00:00
abstract:BACKGROUND AND OBJECTIVES:Pores in the sclera are a candidate pathway for aqueous transport and therefore can be utilized to decrease the intraocular pressure (IOP) in glaucomatous eyes. Since pore formation is a well-known mechanism for stress relaxation in solids, laser-induced creation of pores in cartilage increase...
journal_title:Lasers in surgery and medicine
pub_type: 杂志文章
doi:10.1002/lsm.22202
更新日期:2014-01-01 00:00:00
abstract:BACKGROUND AND OBJECTIVE:A novel, noninvasive technology, utilizing suction-coupled radiofrequency (RF) heating and ultra-short pulse duration, high-voltage electrical pulses was studied for its efficacy and safety on adipose tissue reduction. METHODS:Twenty-one subjects underwent treatment of their abdominal fat once...
journal_title:Lasers in surgery and medicine
pub_type: 临床试验,杂志文章
doi:10.1002/lsm.22223
更新日期:2014-02-01 00:00:00
abstract:BACKGROUND AND OBJECTIVES:Efficacy of topical anesthetics can be enhanced by pretreatment of the skin with ablative fractional lasers. However, little is known about the role of parameters such as laser modality and laser density settings in this technique. Aims of this study were to compare the efficacy of pretreatmen...
journal_title:Lasers in surgery and medicine
pub_type: 杂志文章,随机对照试验
doi:10.1002/lsm.22936
更新日期:2018-10-01 00:00:00
abstract:BACKGROUND:Femtosecond (fs) Laser Ionisation Mass Spectrometry (fs-LIMS) on colon tissues are described and investigated using ionization/fragmentation processes in details to present a new application in this study. Linear Time of Flight (L-TOF) mass analyzer was utilized to investigate paraffin-embedded human tissue ...
journal_title:Lasers in surgery and medicine
pub_type: 杂志文章
doi:10.1002/lsm.23033
更新日期:2019-04-01 00:00:00
abstract::We hypothesized that autologous clot deposited on the luminal surface of laser vascular welds immediately after creation would produce higher time zero bursting pressures that could be achieved in welds perfused with saline alone. To test this hypothesis, we compared bursting pressures of welds created in isolated rab...
journal_title:Lasers in surgery and medicine
pub_type: 杂志文章
doi:10.1002/lsm.1900100414
更新日期:1990-01-01 00:00:00
abstract:BACKGROUND AND OBJECTIVE:Interstitial photodynamic therapy (iPDT) of non-resectable recurrent glioblastoma using 5-aminolevulinic acid (ALA)-induced protoporphyrin IX (PpIX) has shown a promising outcome. It remained unclear, however, to what extent inter- and intra-tumoural differences of PpIX concentrations influence...
journal_title:Lasers in surgery and medicine
pub_type: 临床试验,杂志文章
doi:10.1002/lsm.22126
更新日期:2013-04-01 00:00:00