Alteration of spectral characteristics of human artery wall caused by 476-nm laser irradiation.

Abstract:

:Fluorescence spectroscopy is a promising new technique for discrimination of normal and atherosclerotic arterial tissues. It has been suggested that this technique be used as a guidance system for laser angiosurgery catheters; however, irradiation by 476-nm light can change the spectroscopic properties of arterial tissue. We present studies that establish intensity levels and exposure times at which alterations in tissue spectral properties are minimal. We also investigate the nature of spectral alterations following exposure of normal human aorta to high intensities of 476-nm laser light. Changes in laser-induced fluorescence (LIF) are characterized by two prominent features: the peak fluorescence intensity decreases permanently, and the fluorescence lineshape changes in a largely reversible way. We relate these changes to alterations in individual tissue chromophores: permanent changes in absolute fluorescence intensity are due to irreversible changes in tissue fluorophores, reversible changes in fluorescence lineshape are due alterations in tissue absorbers. A simple kinetic model is used to describe the decrease in absolute fluorescence intensity.

journal_name

Lasers Surg Med

authors

Chaudhry HW,Richards-Kortum R,Kolubayev T,Kittrell C,Partovi F,Kramer JR,Feld MS

doi

10.1002/lsm.1900090606

subject

Has Abstract

pub_date

1989-01-01 00:00:00

pages

572-80

issue

6

eissn

0196-8092

issn

1096-9101

journal_volume

9

pub_type

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