Lymphatic response to depilation-induced inflammation in mouse ear assessed with label-free optical lymphangiography.

Abstract:

BACKGROUND AND OBJECTIVES:Optical microangiography (OMAG) is a noninvasive technique capable of imaging 3D microvasculature. OMAG-based optical lymphangiography has been developed for 3D visualization of lymphatic vessels without the need for exogenous contrast agents. In this study, we utilize the optical lymphangiography to investigate dynamic changes in lymphatic response within skin tissue to depilation-induced inflammation by using mouse ear as a simple tissue model. MATERIALS AND METHODS:A spectral-domain optical coherence tomography (OCT) system is used in this study to acquire volumetric images of mouse ear. The system operates under the ultrahigh-sensitive OMAG scanning protocol with five repetitions for each B frame. An improved adaptive-threshold-based method is proposed to segment lymphatic vessels from OCT microstructure images. Depilation is achieved by placing hair removal lotion on mouse ear pinna for 5 minutes. Three acquisitions are made before depilation, 3-minute and 30-minute post-depilation, respectively. RESULTS:Right after the application of depilation lotion on the skin, we observe that the blind-ended sacs of initial lymphatics are mainly visible in a specific area of the normal tissue. At 5 minutes, more collecting lymphatic vessels start to form, evidenced by their valve structure that only exists in collecting lymphatic vessels. The lymphangiogenesis is almost completed within 8 minutes in the inflammatory tissue. CONCLUSIONS:Our experimental results demonstrate that the OMAG-based optical lymphangiography has great potential to improve the understanding of lymphatic system in response to various physiological conditions, thus would benefit the development of effective therapeutics.

journal_name

Lasers Surg Med

authors

Qin W,Baran U,Wang R

doi

10.1002/lsm.22387

subject

Has Abstract

pub_date

2015-10-01 00:00:00

pages

669-76

issue

8

eissn

0196-8092

issn

1096-9101

journal_volume

47

pub_type

杂志文章
  • Low intensity monochromatic infrared therapy: a preliminary study of the effects of a novel treatment unit upon experimental muscle soreness.

    abstract:BACKGROUND AND OBJECTIVE:The current study (for which ethical approval was obtained) sought to assess the effect of low intensity monochromatic infrared therapy (LIMIRT) on experimentally induced delayed onset muscle soreness (DOMS). STUDY DESIGN/MATERIALS AND METHODS:Healthy volunteers were recruited (n = 24, 12M:12F...

    journal_title:Lasers in surgery and medicine

    pub_type: 临床试验,杂志文章,随机对照试验

    doi:10.1002/1096-9101(2001)28:1<33::AID-LSM1012>3.0.CO

    authors: Glasgow PD,Hill ID,McKevitt AM,Lowe AS,Baxter D

    更新日期:2001-01-01 00:00:00

  • Practical models for light distribution in laser-irradiated tissue.

    abstract::The distribution of light in laser-irradiated biological medium is computed using four different procedures. Values computed with Beer's law of attenuation, Kubelka-Munk theory, the diffusion approximation, and a seven-flux model are compared. The proper conditions for using each model are discussed. ...

    journal_title:Lasers in surgery and medicine

    pub_type: 杂志文章

    doi:10.1002/lsm.1900060603

    authors: Welch AJ,Yoon G,van Gemert MJ

    更新日期:1987-01-01 00:00:00

  • Effect of CO2 laser on the luminal surface of blood vessels in vivo.

    abstract::The use of lasers as surgical tools may open up new possibilities for the treatment of atherosclerotic occlusive vascular disease. However, basic information regarding the effect of laser radiation to the lumen of normal blood vessels is needed prior to clinical application. This work investigates the nature of the CO...

    journal_title:Lasers in surgery and medicine

    pub_type: 杂志文章

    doi:10.1002/lsm.1900030308

    authors: Treat MR,Weld FM,White JV,Forde KA,Fenoglio JJ,L'Esperance F,Voorhees AB

    更新日期:1983-01-01 00:00:00

  • Delivery of light to the skin through ablated conduits.

    abstract:BACKGROUND AND OBJECTIVES:Non-invasive laser skin treatment modalities are generally designed to protect the epidermis by cooling and limiting the laser energy deposition in accordance with skin type. We explore a treatment modality that uses a 2,940 nm Er:YAG laser with high tissue absorption to ablate an array of cha...

    journal_title:Lasers in surgery and medicine

    pub_type: 杂志文章,评审

    doi:10.1002/lsm.22533

    authors: Tanghetti E,Mirkov M,Sierra RA

    更新日期:2017-01-01 00:00:00

  • Application of low-level laser therapy for noninvasive body contouring.

    abstract:BACKGROUND:Low-level laser therapy (LLLT) is a noninvasive treatment for a wide-assortment of medical ailments. A recent application is for noninvasive body slimming. A Level 1 clinical study was completed and recorded a significant reduction in circumferential measurements across waist, hips, and thighs compared to pl...

    journal_title:Lasers in surgery and medicine

    pub_type: 临床试验,杂志文章

    doi:10.1002/lsm.22007

    authors: Jackson RF,Stern FA,Neira R,Ortiz-Neira CL,Maloney J

    更新日期:2012-03-01 00:00:00

  • Dynamic behavior of cryogen spray cooling: effects of spurt duration and spray distance.

    abstract:BACKGROUND AND OBJECTIVES:Cryogen spray cooling (CSC) is used to minimize the risk of epidermal damage during laser dermatologic surgery. Since optimization of CSC permits the safe use of higher light doses, which improves therapeutic outcome in many patients with superficial skin lesions, studies have focused on under...

    journal_title:Lasers in surgery and medicine

    pub_type: 杂志文章

    doi:10.1002/lsm.10133

    authors: Aguilar G,Wang GX,Nelson JS

    更新日期:2003-01-01 00:00:00

  • Photodynamic effects of toluidine blue on human oral keratinocytes and fibroblasts and Streptococcus sanguis evaluated in vitro.

    abstract:BACKGROUND AND OBJECTIVE:Some oral bacteria are susceptible to killing by red light after their sensitization with toluidine blue O (TBO). The photochemotherapy of periodontal disease in vivo would require a therapeutic window where bacteria could be killed without adjacent normal tissue damage. STUDY DESIGN/MATERIALS...

    journal_title:Lasers in surgery and medicine

    pub_type: 杂志文章

    doi:10.1002/(SICI)1096-9101(1996)18:3<253::AID-LSM6>3.

    authors: Soukos NS,Wilson M,Burns T,Speight PM

    更新日期:1996-01-01 00:00:00

  • Human liver autofluorescence: an intrinsic tissue parameter discriminating normal and diseased conditions.

    abstract:BACKGROUND AND OBJECTIVE:Autofluorescence (AF) emission is an intrinsic parameter that can provide real-time information on morpho-functional properties of biological tissue, being strictly related with their biochemical composition and structural organization. The diagnostic potentials of AF-based techniques have been...

    journal_title:Lasers in surgery and medicine

    pub_type: 杂志文章

    doi:10.1002/lsm.20923

    authors: Croce AC,De Simone U,Freitas I,Boncompagni E,Neri D,Cillo U,Bottiroli G

    更新日期:2010-07-01 00:00:00

  • Laser recannalization of atheromatous vessels using fiber optics.

    abstract::A laser endoscope was devised using optic fibers in order to provide a means of visualization and removal of atheromatous plaques from arteries in cadavers and experimental animals. Atheromatous plaques were satisfactorily lased off in a few seconds, although contraction of lased vessels was observed. A self-focusing ...

    journal_title:Lasers in surgery and medicine

    pub_type: 杂志文章

    doi:10.1002/lsm.1900040408

    authors: Ward H

    更新日期:1984-01-01 00:00:00

  • In vitro resistance of articular chondrocytes to 5-Aminolevulinic acid based photodynamic therapy.

    abstract:OBJECTIVE:5-Aminolevulinic acid based photodynamic therapy (5-ALA-PDT) has revealed promising results in the treatment of inflammatory joint diseases due to the sensitivity of inflamed synovial tissue. For 5-ALA-PDT to be safe and beneficial for intra-articular applications, resistance of chondrocytes is essential to p...

    journal_title:Lasers in surgery and medicine

    pub_type: 杂志文章

    doi:10.1002/lsm.20625

    authors: Egli RJ,Di Criscio A,Hempfing A,Schoeniger R,Ganz R,Hofstetter W,Leunig M

    更新日期:2008-04-01 00:00:00

  • A first trial in the clinical application of photodynamic therapy for the prevention of restenosis after coronary-stent placement.

    abstract:BACKGROUND AND OBJECTIVES:The aim of this clinical study was to evaluate the safety of local delivery of a photosensitizer followed by photodynamic therapy (PDT), to determine its effectiveness in reducing in-stent restenosis. STUDY DESIGN/PATIENTS AND METHODS:Porfimer sodium was administered via a local delivery cath...

    journal_title:Lasers in surgery and medicine

    pub_type: 杂志文章

    doi:10.1002/lsm.20018

    authors: Usui M,Miyagi M,Fukasawa S,Hara T,Ueyama N,Nakajima H,Takata R,Sasame A,Tamura K,Naitou Y,Yamashina A

    更新日期:2004-01-01 00:00:00

  • Fractional carbon dioxide laser ablation for the treatment of microcystic lymphatic malformations (lymphangioma circumscriptum) in an adult patient with Klippel-Trenaunay syndrome.

    abstract:BACKGROUND:Lymphangioma circumscriptum (LC) is a rare, benign, vascular malformation of dilated superficial lymphatic channels. Treatment is typically undertaken for cosmetic reasons or complications such as fluid drainage, pain, and infection risk that can negatively impact quality of life. Management of LC remains ch...

    journal_title:Lasers in surgery and medicine

    pub_type: 杂志文章

    doi:10.1002/lsm.22379

    authors: Saluja S,Petersen M,Summers E

    更新日期:2015-09-01 00:00:00

  • A clinical and biological evaluation of a novel, noninvasive radiofrequency device for the long-term reduction of adipose tissue.

    abstract:BACKGROUND AND OBJECTIVE:A novel, noninvasive technology, utilizing suction-coupled radiofrequency (RF) heating and ultra-short pulse duration, high-voltage electrical pulses was studied for its efficacy and safety on adipose tissue reduction. METHODS:Twenty-one subjects underwent treatment of their abdominal fat once...

    journal_title:Lasers in surgery and medicine

    pub_type: 临床试验,杂志文章

    doi:10.1002/lsm.22223

    authors: Boisnic S,Divaris M,Nelson AA,Gharavi NM,Lask GP

    更新日期:2014-02-01 00:00:00

  • Fiber-based laser speckle imaging for the detection of pulsatile flow.

    abstract:BACKGROUND AND OBJECTIVE:In endodontics, a major diagnostic challenge is the accurate assessment of pulp status. In this study, we designed and characterized a fiber-based laser speckle imaging system to study pulsatile blood flow in the tooth. STUDY DESIGN/MATERIALS AND METHODS:To take transilluminated laser speckle ...

    journal_title:Lasers in surgery and medicine

    pub_type: 杂志文章

    doi:10.1002/lsm.22370

    authors: Regan C,Yang BY,Mayzel KC,Ramirez-San-Juan JC,Wilder-Smith P,Choi B

    更新日期:2015-08-01 00:00:00

  • Laser phototherapy as topical prophylaxis against head and neck cancer radiotherapy-induced oral mucositis: comparison between low and high/low power lasers.

    abstract:BACKGROUND AND OBJECTIVE:Oral mucositis is a dose-limiting and painful side effect of radiotherapy (RT) and/or chemotherapy in cancer patients. The purpose of the present study was to analyze the effect of different protocols of laser phototherapy (LPT) on the grade of mucositis and degree of pain in patients under RT....

    journal_title:Lasers in surgery and medicine

    pub_type: 杂志文章,随机对照试验

    doi:10.1002/lsm.20758

    authors: Simões A,Eduardo FP,Luiz AC,Campos L,Sá PH,Cristófaro M,Marques MM,Eduardo CP

    更新日期:2009-04-01 00:00:00

  • Low power laser irradiation alters the rate of regeneration of the rat facial nerve.

    abstract::Low power laser irradiation has been reported to cause biological effects due to the photochemical and/or photophysical action of the radiation. This study determined quantitatively if transcutaneous low power laser irradiation can affect the regeneration of the rat facial nerve. The facial nerve was crushed unilatera...

    journal_title:Lasers in surgery and medicine

    pub_type: 杂志文章

    doi:10.1002/lsm.1900130113

    authors: Anders JJ,Borke RC,Woolery SK,Van de Merwe WP

    更新日期:1993-01-01 00:00:00

  • Enhancement of bonding to enamel and dentin prepared by Er,Cr:YSGG laser.

    abstract:BACKGROUND AND OBJECTIVE:Erbium lasers are potential tools to remove caries and dental hard tissue but bond strengths of composites to those preparations are reported to be lower than conventional methods. The purpose of this study was to evaluate the effect of mechanical excavation and/or chemical alteration on bond s...

    journal_title:Lasers in surgery and medicine

    pub_type: 杂志文章

    doi:10.1002/lsm.20790

    authors: Obeidi A,McCracken MS,Liu PR,Litaker MS,Beck P,Rahemtulla F

    更新日期:2009-08-01 00:00:00

  • In vitro study of the variable square pulse Er:YAG laser cutting efficacy for apicectomy.

    abstract:BACKGROUND AND OBJECTIVES:Variable square pulse (VSP) Er:YAG laser should be quicker than older Er:YAG lasers. The objectives were: (1) comparison of VSP laser and mechanical handpiece efficacy for apicectomy and (2) determination of optimal pulse width/energy/frequency combination. STUDY DESIGN/MATERIALS AND METHODS:...

    journal_title:Lasers in surgery and medicine

    pub_type: 杂志文章

    doi:10.1002/lsm.20168

    authors: Grgurević J,Grgurević L,Miletić I,Karlović Z,Krmek SJ,Anić I

    更新日期:2005-06-01 00:00:00

  • Dynamic pain after laser-assisted-Uvulo-palatoplasty (LAUP) can instantaneously be mitigated by topical regimens.

    abstract:BACKGROUND AND OBJECTIVES:Kenalog application onto surgical wounds has been used to control dynamic pain following Laser-Assisted-Uvulo-Palatoplasty (LAUP) with good results in our department. When the effect was sub-optimal, insufficient ointment over the superior-lateral corner of wounds were always noted. Fulfilling...

    journal_title:Lasers in surgery and medicine

    pub_type: 杂志文章

    doi:10.1002/lsm.21122

    authors: Li LP,Lee SS,Chuang AY,Niddam DM,Tung TH,Chen JK

    更新日期:2011-11-01 00:00:00

  • Comparing and combining light dose fractionation and iron chelation to enhance experimental photodynamic therapy with aminolevulinic acid.

    abstract:BACKGROUND AND OBJECTIVES:Enhancement of photodynamic therapy (PDT) with 5-aminolevulinic acid (ALA) has been demonstrated experimentally using light dose fractionation or CP94 iron chelation. This study extends this research. STUDY DESIGN/MATERIALS AND METHODS:In normal rat colon, CP94 administration and light dose f...

    journal_title:Lasers in surgery and medicine

    pub_type: 杂志文章

    doi:10.1002/lsm.20328

    authors: Curnow A,MacRobert AJ,Bown SG

    更新日期:2006-04-01 00:00:00

  • Nature: a rich source for developing multifunctional agents. Tumor-imaging and photodynamic therapy.

    abstract::The purpose of this review is to call attention in the use of chlorophyll-a and bacteriochlorophyll-a to develop more than 600 photosensitizers (lambda (max) 660 nm-800 nm) during the last 15 years (1990-2005) at the Photodynamic Therapy Center, Roswell Park Cancer Institute, Buffalo. This article mainly includes the ...

    journal_title:Lasers in surgery and medicine

    pub_type: 杂志文章,评审

    doi:10.1002/lsm.20352

    authors: Pandey RK,Goswami LN,Chen Y,Gryshuk A,Missert JR,Oseroff A,Dougherty TJ

    更新日期:2006-06-01 00:00:00

  • Monte Carlo simulations for EndoBronchial Photodynamic Therapy: the influence of variations in optical and geometrical properties and of realistic and eccentric light sources.

    abstract:BACKGROUND AND OBJECTIVE:Light dosimetry for endobronchial photodynamic therapy is not very advanced to date. This study investigates the dependency of the fluence rate distribution in the bronchial wall on several parameters. STUDY DESIGN/MATERIALS AND METHODS:A Monte Carlo model is employed for the illumination of a...

    journal_title:Lasers in surgery and medicine

    pub_type: 杂志文章

    doi:10.1002/(sici)1096-9101(1998)22:4<193::aid-lsm3>3.

    authors: Murrer LH,Marijnissen HP,Star WM

    更新日期:1998-01-01 00:00:00

  • Endoscopic lithotripsy with the holmium:YAG laser.

    abstract:BACKGROUND AND OBJECTIVE:The holmium:YAG (Ho:YAG) laser can be used not only for soft tissue but also for hard tissue such as urinary calculi. The objective of this study was to assess the usefulness of the Ho:YAG laser for endoscopic lithotripsy in patients with urinary tract stone. STUDY DESIGN/MATERIASL AND METHODS:...

    journal_title:Lasers in surgery and medicine

    pub_type: 杂志文章

    doi:10.1002/(sici)1096-9101(1999)25:5<389::aid-lsm4>3.

    authors: Matsuoka K,Iida S,Inoue M,Yoshii S,Arai K,Tomiyasu K,Noda S

    更新日期:1999-01-01 00:00:00

  • Thermal modeling and experimental validation of human hair and skin heated by broadband light.

    abstract:BACKGROUND AND OBJECTIVE:In recent years, selective photothermolysis (PT) based on broadband light and assisted by skin vacuum (Photopneumatic Technology or PPx, by Aesthera) was introduced into the aesthetic market for epilation (hair removal) procedures. The present study aims to: (a) determine the overall effect of ...

    journal_title:Lasers in surgery and medicine

    pub_type: 杂志文章

    doi:10.1002/lsm.20743

    authors: Sun F,Chaney A,Anderson R,Aguilar G

    更新日期:2009-02-01 00:00:00

  • A split-face study of intense pulsed light on photoaging skin in Chinese population.

    abstract:BACKGROUND AND OBJECTIVES:Intense pulsed light (IPL) is regarded as the gold standard of nonablative photo-rejuvenation. Yet there is still a need to observe its efficacy and safety on dark skin using a split-face module. STUDY DESIGN/MATERIALS AND METHODS:Twenty-four Chinese women with photoaging were enrolled in thi...

    journal_title:Lasers in surgery and medicine

    pub_type: 杂志文章,随机对照试验

    doi:10.1002/lsm.20889

    authors: Li YH,Wu Y,Chen JZ,Zhu X,Xu YY,Chen J,Dong GH,Gao XH,Chen HD

    更新日期:2010-02-01 00:00:00

  • The potential of phototherapy to reduce body fat, insulin resistance and "metabolic inflexibility" related to obesity in women undergoing weight loss treatment.

    abstract:BACKGROUND AND OBJECTIVE:The metabolic flexibility is often impaired in diseases associated with obesity, and many studies are based on the hypothesis that dysfunction in peripheral tissues such as skeletal muscle, liver and adipose tissue represent the etiology of development of metabolic inflexibility. Experimental e...

    journal_title:Lasers in surgery and medicine

    pub_type: 杂志文章,随机对照试验

    doi:10.1002/lsm.22395

    authors: Sene-Fiorese M,Duarte FO,de Aquino Junior AE,Campos RM,Masquio DC,Tock L,de Oliveira Duarte AC,Dâmaso AR,Parizotto NA,Bagnato VS

    更新日期:2015-10-01 00:00:00

  • Intraoperative Raman spectroscopy of soft tissue sarcomas.

    abstract:BACKGROUND AND OBJECTIVE:Soft tissue sarcomas (STS) are a rare and heterogeneous group of malignant tumors that are often treated through surgical resection. Current intraoperative margin assessment methods are limited and highlight the need for an improved approach with respect to time and specificity. Here we investi...

    journal_title:Lasers in surgery and medicine

    pub_type: 临床试验,杂志文章

    doi:10.1002/lsm.22564

    authors: Nguyen JQ,Gowani ZS,O'Connor M,Pence IJ,Nguyen TQ,Holt GE,Schwartz HS,Halpern JL,Mahadevan-Jansen A

    更新日期:2016-10-01 00:00:00

  • Prospective study of port wine stain treatment with dye laser: comparison of two wavelengths (585 nm vs. 595 nm) and two pulse durations (0.5 milliseconds vs. 20 milliseconds).

    abstract:BACKGROUND AND OBJECTIVES:The conventional pulsed-dye laser (wavelength 585 nm, pulse duration 0.5 milliseconds) is seen as the standard treatment for port wine stains (PWS). Using the pulsed-dye laser at wavelengths of 590, 595, and 600 nm and at varying pulse durations of 1.5-40 milliseconds is one of the newest deve...

    journal_title:Lasers in surgery and medicine

    pub_type: 临床试验,杂志文章,随机对照试验

    doi:10.1002/lsm.20003

    authors: Greve B,Raulin C

    更新日期:2004-01-01 00:00:00

  • Enhanced tumor control following sequential treatments of photodynamic therapy (PDT) and localized microwave hyperthermia in vivo.

    abstract::Photodynamic therapy (PDT), or photoradiation therapy (PRT), utilizing hematoporphyrin derivative (HPD) as photosensitizer and an argon-dye laser system as the light source, was used alone and in combination with localized microwave hyperthermia (2450 MHz) to treat axillary tumors of the SMT-F mammary carcinoma in mic...

    journal_title:Lasers in surgery and medicine

    pub_type: 杂志文章

    doi:10.1002/lsm.1900040111

    authors: Waldow SM,Henderson BW,Dougherty TJ

    更新日期:1984-01-01 00:00:00

  • Cryogen spray cooling in laser dermatology: effects of ambient humidity and frost formation.

    abstract:BACKGROUND AND OBJECTIVE:Dynamics of cryogen spray deposition, water condensation and frost formation is studied in relationship to cooling rate and efficiency of cryogen spray cooling (CSC) in combination with laser dermatologic surgery. STUDY DESIGN/MATERIALS AND METHODS:A high-speed video camera was used to image t...

    journal_title:Lasers in surgery and medicine

    pub_type: 杂志文章

    doi:10.1002/lsm.1076

    authors: Majaron B,Kimel S,Verkruysse W,Aguilar G,Pope K,Svaasand LO,Lavernia EJ,Nelson JS

    更新日期:2001-01-01 00:00:00