Laser treatments on skin enhancing and controlling transdermal delivery of 5-fluorouracil.

Abstract:

BACKGROUND AND OBJECTIVE:Laser ablation of stratum corneum (SC) enhances transdermal delivery of hydrophilic drugs. The influence of the infrared (IR) (lambda = 1,064 nm), visible (lambda = 532 nm), and ultraviolet (UV) (lambda = 355 nm) radiations of a Nd:YAG laser on transdermal delivery of 5-Fluorouracil (5-Fu) across skin was studied in vitro. MATERIALS AND METHODS:Pinna skin of the inner side of rabbit ear, was used for the skin permeation. The light source for laser treatment was a Q-switched Nd:YAG laser (Lotis TII SL-2132). Ablation thresholds were estimated by using a photoacoustic technique. In addition, permeation study, and morphological and structural skin examination by histology and differential scanning calorimetry (DSC) were carried out. RESULTS:A significant increase in the permeation of 5-Fu across skin pre-treatment with the three different wavelengths studied was obtained. Since irradiation at 1,064 nm allows deep penetration of the radiation, collagen fibers were affected [7.7 J/cm(2) (15 Hz)]. Visible radiation of Nd:YAG laser showed the wider range of fluences (3-8.4 J/cm(2) at 15 Hz) to enhance skin delivery of 5-Fu, without risk of skin lesion. UV radiation required minor energy contribution to produce the same effects within a narrower range of fluences [0.3 J/cm(2) (5 Hz)-1.5 J/cm(2) (15 Hz)] so the process is less controlled and this radiation shows greater impact on the lipidic structure than visible and IR radiations. CONCLUSIONS:Use of the visible radiation of a Nd:YAG laser is a good method for improving the efficacy of topical chemotherapy of 5-Fu.

journal_name

Lasers Surg Med

authors

Gómez C,Costela A,García-Moreno I,Llanes F,Teijón JM,Blanco D

doi

10.1002/lsm.20590

subject

Has Abstract

pub_date

2008-01-01 00:00:00

pages

6-12

issue

1

eissn

0196-8092

issn

1096-9101

journal_volume

40

pub_type

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