Abstract:
:To clarify the molecular nature of the pathogenesis in X-linked Emery-Dreifuss muscular dystrophy (EDMD), we monitored the expression of 2400 genes in control and EDMD fibroblasts by using complementary DNA (cDNA) microarray techniques. A total of 60 genes whose expression was altered in EDMD fibroblasts when compared with control fibroblasts were identified. Twenty-eight genes whose expression was altered with the emerin deficiency were rescued by infection with a recombinant adenovirus expressing emerin. The altered expression in five genes, including the lamin A/C gene, was confirmed by reverse transcription-polymerase chain reaction. Our preliminary results suggest a correlation between disease similarity and gene expression. We conclude that the cDNA microarray is a very efficient tool to clarify genetic and pathological features of diseases.
journal_name
Muscle Nervejournal_title
Muscle & nerveauthors
Tsukahara T,Tsujino S,Arahata Kdoi
10.1002/mus.10085subject
Has Abstractpub_date
2002-06-01 00:00:00pages
898-901issue
6eissn
0148-639Xissn
1097-4598journal_volume
25pub_type
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