Cryopreservation of peripheral nerve grafts.

Abstract:

:The utilization of viable biological nerve graft substitutes and nerve allografts raises the problem of nerve storage. To clarify this, rat sciatic nerve segments were harvested and stored in Dulbecco's modified eagle medium. The segments were divided into three groups. In the first group, no cryoprotectant was added, whereas the second had 10% dimethyl sulfoxide (DMSO) added as cryoprotectant. These two groups of nerve segments were subjected to controlled freezing. In a third group, segments were frozen uncontrolled in liquid nitrogen (-196 degrees C). All nerves were replanted orthotopically. Fresh conventional autografts (fourth group) served as control group. Histologically, freezing did not affect the structural elements such as basal lamina tubes and perineurial tissue. Morphometrically, all cryopreserved grafts had significantly reduced axon counts and less myelinization than did controls. Cryoprotected nerves (group 2) showed no different morphometric parameters compared with the group without DMSO (group 1). Controlled freezing was superior to uncontrolled freezing (group 3). Impaired regeneration was attributed mainly to delayed Wallerian degeneration and slower revascularization. Moreover, decreased survival of resident Schwann cells in the graft may impair regeneration due to the lack of neurotrophic, neurotropic, and attachment factors in early regeneration. Grafts subjected to controlled freezing support axonal regeneration to a certain extent, but further studies are required to assess various cooling patterns, cryoprotectants, and graft revascularization.

journal_name

Muscle Nerve

journal_title

Muscle & nerve

authors

Fansa H,Lassner F,Kook PH,Keilhoff G,Schneider W

doi

10.1002/1097-4598(200008)23:8<1227::aid-mus11>3.0.

subject

Has Abstract

pub_date

2000-08-01 00:00:00

pages

1227-33

issue

8

eissn

0148-639X

issn

1097-4598

pii

10.1002/1097-4598(200008)23:8<1227::AID-MUS11>3.0.

journal_volume

23

pub_type

杂志文章
  • Continuous motor unit activity confined to the upper extremities.

    abstract::We report a patient with clinical vermiform motor activity, muscle cramps, delayed relaxation of grip, and continuous motor unit discharges who developed mild symmetric symptoms in the upper extremities following a viral infection. Treatment with carbamazepine produced considerable symptomatic improvement. Needle elec...

    journal_title:Muscle & nerve

    pub_type: 杂志文章

    doi:10.1002/mus.880110310

    authors: Tandan R,Fries TJ

    更新日期:1988-03-01 00:00:00

  • Paradoxical effect of isoproterenol on hamster hereditary polymyopathy.

    abstract::Isoproterenol (ISO), a potent beta-adrenoreceptor agonist, was found to interfere with the development and progression of hamster hereditary polymyopathy. Cytoprotection involved both skeletal and heart muscles with reduced myofibrillar degeneration, phagocytosis, and an unusual scarring process rarely seen at this st...

    journal_title:Muscle & nerve

    pub_type: 杂志文章

    doi:10.1002/mus.880060603

    authors: Jasmin G,Proschek L

    更新日期:1983-07-01 00:00:00

  • Origin of surface motor unit potentials in hypothenar motor unit number estimation.

    abstract:INTRODUCTION:Far-field potentials (FFPs) from muscles other than the abductor digiti minimi (ADM) may interfere with motor unit number estimation (MUNE) from that muscle. METHODS:We identified the origin of each surface motor unit potential (SMUP) during hypothenar MUNE using the multiple point stimulation method in 2...

    journal_title:Muscle & nerve

    pub_type: 杂志文章

    doi:10.1002/mus.23746

    authors: Kawamura Y,Sonoo M,Higashihara M,Chiba T,Hatanaka Y

    更新日期:2013-08-01 00:00:00

  • A multicenter, retrospective medical record review of X-linked myotubular myopathy: The recensus study.

    abstract:INTRODUCTION:X-linked myotubular myopathy (XLMTM), characterized by severe hypotonia, weakness, respiratory distress, and early mortality, is rare and natural history studies are few. METHODS:RECENSUS is a multicenter chart review of male XLMTM patients characterizing disease burden and unmet medical needs. Data were ...

    journal_title:Muscle & nerve

    pub_type: 杂志文章,多中心研究

    doi:10.1002/mus.26018

    authors: Beggs AH,Byrne BJ,De Chastonay S,Haselkorn T,Hughes I,James ES,Kuntz NL,Simon J,Swanson LC,Yang ML,Yu ZF,Yum SW,Prasad S

    更新日期:2018-04-01 00:00:00

  • Association between pulmonary function and left ventricular volume and function in duchenne muscular dystrophy.

    abstract:INTRODUCTION:Duchenne muscular dystrophy (DMD) is characterized by absence of the subsarcolemmal protein dystrophin, present in skeletal muscles and cardiomyocytes. We hypothesized that progressive respiratory and left ventricular (LV) insufficiencies in DMD could be parallel and interrelated phenomena. METHODS:We con...

    journal_title:Muscle & nerve

    pub_type: 杂志文章

    doi:10.1002/mus.26623

    authors: Khokhar A,Nair A,Midya V,Kumar A,Sinharoy A,Ahmad TA,Abu-Hasan M,Mondal P

    更新日期:2019-09-01 00:00:00

  • Peptides and neuromas: calcitonin gene-related peptide, substance P, and mast cells in a mechanosensitive human sural neuroma.

    abstract::We examined and compared a mechanosensitive human sural neuroma and a contralateral sural nerve collected simultaneously from a patient involved in a diabetic neuropathy research protocol. Using indirect immunofluorescence staining. we identified a striking difference in the content within axons of two neuropeptides, ...

    journal_title:Muscle & nerve

    pub_type: 杂志文章

    doi:10.1002/(sici)1097-4598(199707)20:7<875::aid-mus12

    authors: Zochodne DW,Theriault M,Sharkey KA,Cheng C,Sutherland G

    更新日期:1997-07-01 00:00:00

  • Nerve ultrasound in electrophysiologically verified tarsal tunnel syndrome.

    abstract:INTRODUCTION:Tarsal tunnel syndrome (TTS) arises from tibial nerve damage under the flexor retinaculum of the fibro-osseus tunnel at the medial malleolus. It is notoriously difficult to diagnose, as many other foot pathologies result in a similar clinical picture. We examined the additional value of nerve ultrasound in...

    journal_title:Muscle & nerve

    pub_type: 杂志文章

    doi:10.1002/mus.24963

    authors: Samarawickrama D,Therimadasamy AK,Chan YC,Vijayan J,Wilder-Smith EP

    更新日期:2016-06-01 00:00:00

  • Peripheral nerve atrophy together with higher cerebrospinal fluid progranulin indicate axonal damage in amyotrophic lateral sclerosis.

    abstract:INTRODUCTION:We aimed to investigate whether sonographic peripheral cross-sectional nerve area (CSA) and progranulin (PGRN), a neuritic growth factor, are related to each other and whether they interact to predict clinical and paraclinical measures in amyotrophic lateral sclerosis (ALS). METHODS:We included 55 ALS pat...

    journal_title:Muscle & nerve

    pub_type: 杂志文章

    doi:10.1002/mus.25682

    authors: Schreiber S,Debska-Vielhaber G,Abdulla S,Machts J,Schreiber F,Kropf S,KÖrtvelyessy P,KÖrner S,Kollewe K,Petri S,Dengler R,Kunz WS,Nestor PJ,Vielhaber S

    更新日期:2018-02-01 00:00:00

  • Continuous intrathecal baclofen infusions induced a marked increase of the transcranially evoked silent period in a patient with generalized dystonia.

    abstract::We observed a marked prolongation of the transcranially evoked silent period during continuous intrathecal administration of high doses of the gamma-aminobutyric acid (GABA)B receptor agonist baclofen in a patient with generalized dystonia. Size of motor evoked potentials and central conduction time remained unchanged...

    journal_title:Muscle & nerve

    pub_type: 杂志文章

    doi:10.1002/(sici)1097-4598(199809)21:9<1209::aid-mus1

    authors: Siebner HR,Dressnandt J,Auer C,Conrad B

    更新日期:1998-09-01 00:00:00

  • Pattern of muscle strength improvement after IVIg therapy in Multifocal Motor Neuropathy.

    abstract:INTRODUCTION:In multifocal motor neuropathy (MMN), knowledge about the pattern of treatment response in a wide spectrum of muscle groups, distal as well as proximal, after intravenous immunoglobulin (IVIg) initiation is lacking. METHODS:Hand-held dynamometry data of 11 upper and lower limb muscles, from 47 patients wi...

    journal_title:Muscle & nerve

    pub_type: 杂志文章

    doi:10.1002/mus.27185

    authors: Bakers JNE,van Eijk RPA,van den Berg LH,Visser-Meily JMA,Beelen A

    更新日期:2021-01-26 00:00:00

  • Use of a catchlike property of human skeletal muscle to reduce fatigue.

    abstract::This study compared the force output produced by variable frequency, short-duration trains (VFTs) of electrical pulses with the forces produced by constant frequency, short-duration trains (CFTs). Human quadriceps femoris muscle was stimulated with a 300-msec train of pulses once every second for 180 seconds. Each sub...

    journal_title:Muscle & nerve

    pub_type: 临床试验,杂志文章,随机对照试验

    doi:10.1002/mus.880140909

    authors: Binder-Macleod SA,Barker CB 3rd

    更新日期:1991-09-01 00:00:00

  • Electrical stimulation impairs early functional recovery and accentuates skeletal muscle atrophy after sciatic nerve crush injury in rats.

    abstract::Neuromuscular recovery after peripheral nerve lesion depends on the regeneration of severed axons that re-establish their functional connection with the denervated muscle. The aim of this study was to determine the effects of electrical stimulation (ES) on the neuromuscular recovery after nerve crush injury in rats. E...

    journal_title:Muscle & nerve

    pub_type: 杂志文章

    doi:10.1002/mus.21549

    authors: Gigo-Benato D,Russo TL,Geuna S,Domingues NR,Salvini TF,Parizotto NA

    更新日期:2010-05-01 00:00:00

  • Modeling functional decline over time in sporadic inclusion body myositis.

    abstract:INTRODUCTION:The ability to individualize recommendations or expectations of disease progression based on a patient's unique characteristics has merit for use in sporadic inclusion body myositis (sIBM). METHODS:Fifty-five subjects with sIBM completed a battery of strength and functional outcomes at 2 study visits. The...

    journal_title:Muscle & nerve

    pub_type: 杂志文章

    doi:10.1002/mus.25373

    authors: Alfano LN,Yin H,Dvorchik I,Maus EG,Flanigan KM,Mendell JR,Lowes LP

    更新日期:2017-04-01 00:00:00

  • Systematic test of neurotoxin dose and volume on muscle function in a rat model.

    abstract:INTRODUCTION:Onabotulinum toxin serotype A (BT-A) is used for a variety of motor and sensory disorders related to abnormal muscle activity. METHODS:We developed a high-resolution rodent model to allow precise determination of the effect of BT-A dose (measured in units) and injectate volume (measured in μl) on the effi...

    journal_title:Muscle & nerve

    pub_type: 杂志文章

    doi:10.1002/mus.23983

    authors: Hulst JB,Minamoto VB,Lim MB,Bremner SN,Ward SR,Lieber RL

    更新日期:2014-05-01 00:00:00

  • Serum CK, calcium, magnesium, and oxidative phosphorylation in mdx mouse muscular dystrophy.

    abstract::Serum creatine kinase (CK) activity, calcium (Ca) and magnesium (Mg) contents of skeletal muscle and isolated mitochondria, as well as oxidative phosphorylation of X-linked muscular dystrophic (mdx) mice were compared with normal control animals at ages 5, 10, and 23 weeks. Serum CK is elevated in mdx mice at all ages...

    journal_title:Muscle & nerve

    pub_type: 杂志文章

    doi:10.1002/mus.880110809

    authors: Glesby MJ,Rosenmann E,Nylen EG,Wrogemann K

    更新日期:1988-08-01 00:00:00

  • Facing the diagnostic challenge: Nerve ultrasound in diabetic patients with neuropathic symptoms.

    abstract:INTRODUCTION:We describe the nerve ultrasound findings in patients with type II diabetes mellitus who have neuropathic symptoms and signs. METHODS:Fifty-five healthy controls and 44 diabetic patients underwent clinical, sonographic, and electrophysiological evaluation. Patients were studied at a mean of 14.3 years aft...

    journal_title:Muscle & nerve

    pub_type: 杂志文章

    doi:10.1002/mus.24981

    authors: Pitarokoili K,Kerasnoudis A,Behrendt V,Labedi A,Ayzenberg I,Gold R,Yoon MS

    更新日期:2016-06-01 00:00:00

  • Ethical review of research involving human subjects: when and why is IRB review necessary?

    abstract::Requirements for ethical review of research involving human subjects are based on widely accepted international standards that are implemented by various national regulations and institutional policies. In the United States, most human research is reviewed by institutional review boards (IRBs) applying federal standar...

    journal_title:Muscle & nerve

    pub_type: 杂志文章,评审

    doi:10.1002/mus.10398

    authors: Wagner RM

    更新日期:2003-07-01 00:00:00

  • A novel points system to predict the prognosis of ulnar neuropathy at the elbow.

    abstract:INTRODUCTION:In this study we aimed to identify prognostic factors of ulnar neuropathy at the elbow (UNE) and developed a scoring system to establish the prognosis. METHODS:We collected baseline clinical, electrophysiologic, and ultrasonographic data from 2 cohorts. The outcomes for all patients were determined on fol...

    journal_title:Muscle & nerve

    pub_type: 杂志文章

    doi:10.1002/mus.25406

    authors: Beekman R,Zijlstra W,Visser LH

    更新日期:2017-05-01 00:00:00

  • Spontaneous electromyographic activity of the tongue in amyotrophic lateral sclerosis.

    abstract:INTRODUCTION:Detection of denervation in muscles in the craniobulbar area is important to assure widespread lower motor neuron involvement in the diagnosis of amyotrophic lateral sclerosis (ALS). The value of spontaneous activity analysis in needle electromyography (EMG) of the tongue has been questioned in the recent ...

    journal_title:Muscle & nerve

    pub_type: 杂志文章

    doi:10.1002/mus.23781

    authors: Tankisi H,Otto M,Pugdahl K,Fuglsang-Frederiksen A

    更新日期:2013-08-01 00:00:00

  • Effects of insulin on protein synthesis in muscles from normal and dystrophic mice.

    abstract::Protein synthesis in soleus and extensor digitorum longus (EDL) muscles was measured in vitro to test the hypothesis that the lack of muscle protein accumulation in dystrophic conditions could be caused by a reduced sensitivity to insulin. We demonstrate that physiological insulin concentrations stimulate protein synt...

    journal_title:Muscle & nerve

    pub_type: 杂志文章

    doi:10.1002/mus.880060709

    authors: Ballard FJ,Nield MK,Tomas FM

    更新日期:1983-09-01 00:00:00

  • Ultrasonography of sciatic nerve endometriosis.

    abstract:INTRODUCTION:We describe the ultrasonographic findings of sciatic nerve endometriosis. METHODS:Two premenopausal women with catamenial sciatica symptoms were examined, the first without a history of endometriosis, the second with previously confirmed endometriosis of the ovary. Ultrasonography, extending from the scia...

    journal_title:Muscle & nerve

    pub_type: 杂志文章

    doi:10.1002/mus.25152

    authors: Arányi Z,Polyák I,Tóth N,Vermes G,Göcsei Z

    更新日期:2016-09-01 00:00:00

  • Surgery for peripheral nerve and brachial plexus injuries or other nerve lesions.

    abstract::The selection of patients who will benefit from peripheral nerve surgery continues to evolve. The evaluation of patients with peripheral nerve problems, and the indications and timing of surgery are reviewed. This includes patients with transections, lesions in continuity, entrapments, tumors, injection injuries, and ...

    journal_title:Muscle & nerve

    pub_type: 杂志文章,评审

    doi:10.1002/(sici)1097-4598(200005)23:5<680::aid-mus4>

    authors: Spinner RJ,Kline DG

    更新日期:2000-05-01 00:00:00

  • Enrichment of the R77C alpha-sarcoglycan gene mutation in Finnish LGMD2D patients.

    abstract::Limb-girdle muscular dystrophy 2D (LGMD2D) is caused by mutations in the alpha-sarcoglycan gene (SGCA). The most frequently reported mutation, 229CGC>TGC (R77C) in exon 3 of SGCA, results in the substitution of arginine by cysteine. We present here the clinical, immunohistochemical, and genetic data of 11 Finnish pati...

    journal_title:Muscle & nerve

    pub_type: 杂志文章

    doi:10.1002/mus.20267

    authors: Hackman P,Juvonen V,Sarparanta J,Penttinen M,Aärimaa T,Uusitalo M,Auranen M,Pihko H,Alén R,Junes M,Lönnqvist T,Kalimo H,Udd B

    更新日期:2005-02-01 00:00:00

  • An anatomic study of the Martin-Gruber anastomosis: electrodiagnostic implications.

    abstract::This study aimed to clarify the morphologic variations of the Martin-Gruber anastomosis (MGA) by tracing the anastomotic fascicles. We used 102 upper limbs, and MGA was found in 39.2%. Among 12 instances of MGA between the branches innervating the flexor digitorum profundus muscle, eight anastomotic branches solely in...

    journal_title:Muscle & nerve

    pub_type: 杂志文章

    doi:10.1002/mus.20141

    authors: Lee KS,Oh CS,Chung IH,Sunwoo IN

    更新日期:2005-01-01 00:00:00

  • Direct dystrophin and reporter gene transfer into dog muscle in vivo.

    abstract::Bacterial beta-galactosidase cDNA was injected without lipofectin into 41 sites in dog muscle and expression was seen in 22 of them. The cDNA and lipofectin was injected into 35 similar sites and expression was seen in 21. Expression was seen in a maximum of 2.5% of muscle fibers and 23.21% of nonmuscle cells. A total...

    journal_title:Muscle & nerve

    pub_type: 杂志文章

    doi:10.1002/(sici)1097-4598(199802)21:2<159::aid-mus2>

    authors: Howell JM,Fletcher S,O'Hara A,Johnsen RD,Lloyd F,Kakulas BA

    更新日期:1998-02-01 00:00:00

  • Randomized controlled trial of strength training in post-polio patients.

    abstract::Many post-polio patients develop new muscle weakness decades after the initial illness. However, its mechanism and treatment are controversial. The purpose of this study was to test the hypotheses that: (1) after strength training, post-polio patients show strength improvement comparable to that seen in the healthy el...

    journal_title:Muscle & nerve

    pub_type: 临床试验,杂志文章,随机对照试验

    doi:10.1002/mus.10327

    authors: Chan KM,Amirjani N,Sumrain M,Clarke A,Strohschein FJ

    更新日期:2003-03-01 00:00:00

  • Plasticity of dynamic muscle performance with strength training in elderly humans.

    abstract::Data are scarce relating to the plasticity with strength training of dynamic muscle performance in older humans. Hence, we investigated alterations in the torque-velocity relation with strength training in old age, and their origin. Knee extension and leg-press exercises were performed three times per week for 14 week...

    journal_title:Muscle & nerve

    pub_type: 杂志文章

    doi:10.1002/mus.20275

    authors: Reeves ND,Maganaris CN,Narici MV

    更新日期:2005-03-01 00:00:00

  • Fibrillation and accelerated AChR degradation in long-term muscle organ culture.

    abstract::Evaluation of the precise molecular dynamics of endplate maintenance and reorganization has been limited by the lack of available in vitro preparations. We describe an organ culture preparation of mouse diaphragm muscle which permits long-term maintenance of muscle viability. Spontaneous fibrillations, increased level...

    journal_title:Muscle & nerve

    pub_type: 杂志文章

    doi:10.1002/mus.880141012

    authors: Wetzel DM,Salpeter MM

    更新日期:1991-10-01 00:00:00

  • Familial hypertrophic cardiomyopathy and muscle carnitine deficiency.

    abstract::Five members of the same family, along three generations, presented with hypertrophic cardiomyopathy. Neurological examination, muscle strength, electromyography, and serum creatine kinase were normal. Skeletal muscle biopsy showed abnormal lipid accumulation and carnitine deficiency. In three patients the cardiac sym...

    journal_title:Muscle & nerve

    pub_type: 杂志文章

    doi:10.1002/mus.880130303

    authors: Bautista J,Rafel E,Martinez A,Sainz I,Herrera J,Segura L,Chinchon I

    更新日期:1990-03-01 00:00:00

  • Differences in excitability properties of FDI and ADM motor axons.

    abstract::The first dorsal interosseous (FDI) and abductor digiti minimi (ADM) muscles are innervated by the same ulnar nerve, but studies have shown that the former is much more severely affected in amyotrophic lateral sclerosis. In this study, threshold tracking was used to investigate whether membrane properties differ betwe...

    journal_title:Muscle & nerve

    pub_type: 杂志文章

    doi:10.1002/mus.21107

    authors: Bae JS,Sawai S,Misawa S,Kanai K,Isose S,Kuwabara S

    更新日期:2009-03-01 00:00:00