Abstract：
:With the system described specific features in a histological section such as neuronal degeneration, specific cell types, or the silver grains of autoradiography can be accurately localized. It is simple to operate, and allows for data storage and retrieval. The system consists of a Zeiss Universal microscope equipped with stepping motor drives on the X and Y axes and interfaced to a PDP-12 computer equipped with a printer/plotter. The user controls the system through a joystick control box and teletype. In spite of magnification changes all points in the field remain constant relative to a point of origin selected at the beginning of the mapping sequence. Therefore, the outline and major features of a section are entered at low magnification while the detail of neuronal degeneration, for example, is added in its precise location at a higher magnification. The plotting of points can be done in either random fashion or in a systemized series of horizontal or vertical scans. A graphic display is available throughout the procedure and all maps are both printed on a Versatec printer/plotter and stored on a disk for future recall and analysis. Use of the system in plotting neuronal degeneration is described.
journal_name
J Neurosci Methodsjournal_title
Journal of neuroscience methodsauthors
Forbes DJ,Petry RWdoi
10.1016/0165-0270(79)90007-4subject
Has Abstractpub_date
1979-03-01 00:00:00pages
77-94issue
1eissn
0165-0270issn
1872-678Xpii
0165-0270(79)90007-4journal_volume
1pub_type
杂志文章abstract：BACKGROUND:Brain parcellation is important for exploiting neuroimaging data. Variability in physiology between individuals has led to the need for data-driven approaches to parcellation, with recent research focusing on simultaneously estimating and partitioning the network structure of the brain. NEW METHOD:We view d...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2020.108628
更新日期：2020-04-01 00:00:00
abstract：:The most interesting property of neurons is their long-distance propagation of signals as spiking action potentials. Since 1993, Neurobasal/B27 has been used as a serum-free medium optimized for hippocampal neuron survival. Neurons on microelectrode arrays (MEA) were used as an assay system to increase spontaneous spi...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2008.01.009
更新日期：2008-05-30 00:00:00
abstract：:Selective and simultaneous voltammetric analysis of catechols and indoles in vivo and in vitro has until now been feasible only by means of 'slow' scanning methods (scan speed in tens of seconds) such as differential pulse (DPV) and differential normal pulse voltammetry in conjunction with electrically and/or chemical...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/0165-0270(94)00043-g
更新日期：1995-09-01 00:00:00
abstract：:We have developed a new method for culturing cells that maintains their health and sterility for many months. Using conventional techniques, primary neuron cultures seldom survive more than 2 months. Increases in the osmotic strength of media due to evaporation are a large and underappreciated contributor to the gradu...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/s0165-0270(01)00412-5
更新日期：2001-09-30 00:00:00
abstract：:Frequency analyses of EEG data yield large data sets, which are high-dimensional and have to be evaluated statistically without a large number of false positive statements. There exist several methods to deal with this problem in multiple comparisons. Knowing the number of true hypotheses increases the power of some m...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2007.12.013
更新日期：2008-05-15 00:00:00
abstract：:The relationship between pressure, ejection duration and volume ejected was experimentally determined in vitro for micropipettes with different external tip diameters. The relationship between ejection duration and ejected volume is linear in the steady state (i.e. with ejection durations of 1 s or longer) and at suff...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/0165-0270(84)90021-9
更新日期：1984-04-01 00:00:00
abstract：:The properties of 4 types of carbon electrodes designed for use as in vivo sensors of easily oxidized species in the mammalian brain have been evaluated in aqueous solutions at physiological pH. The electrodes are formed from a graphite-epoxy mixture, carbon paste, or carbon fibers, and have the geometries of a disk o...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/0165-0270(84)90058-x
更新日期：1984-03-01 00:00:00
abstract：:An intra-arterially perfused working heart-brainstem preparation (WH-BP) was developed to allow studies into functionally identified cardiovascular and respiratory neurones in an in vitro milieu. This report provides that first description of this preparation. Evidence is presented indicating that the WH-BP: (1) spont...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/0165-0270(95)00147-6
更新日期：1996-03-01 00:00:00
abstract：:An opto-electronic system has been developed to measure movements of insect appendages. It is made from a mirror-lens and a linear position-sensitive photodiode. The design of the mirror-lens has been exploited to axially mount a high intensity halogen light source in front of the mirror-lens. The system monitors a re...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/s0165-0270(00)00255-7
更新日期：2000-07-31 00:00:00
abstract：:A new technique is described for accurate, reliable measurement of eyeball retraction in the rabbit. A narrow film strip, on which a linear light intensity grating has been exposed, is attached to a contact lens which is placed on the animal's cornea. The other end of the intensity grating slides between a photodiode ...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/0165-0270(84)90077-3
更新日期：1984-01-01 00:00:00
abstract：:The physical basis for electrical stimulation of excitable tissue, as used by electrophysiological researchers and clinicians in functional electrical stimulation, is presented with emphasis on the fundamental mechanisms of charge injection at the electrode/tissue interface. Faradaic and non-Faradaic charge transfer m...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章,评审
doi：10.1016/j.jneumeth.2004.10.020
更新日期：2005-02-15 00:00:00
abstract：:Functional magnetic resonance imaging (fMRI) based on BOLD signal has been used to indirectly measure the local neural activity induced by cognitive tasks or stimulation. Most fMRI data analysis is carried out using the general linear model (GLM), a statistical approach which predicts the changes in the observed BOLD ...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2010.09.005
更新日期：2011-01-15 00:00:00
abstract：BACKGROUND:Independent component analysis (ICA) has been widely used for blind source separation in the field of medical imaging. However, despite of previous substantial efforts, the stability of ICA components remains a critical issue which has not been adequately addressed, despite numerous previous efforts. Most cr...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2020.109013
更新日期：2020-12-11 00:00:00
abstract：:The image-combining computer microscope is a new configuration of light microscope and computer graphics instrumentation which provides remarkable morphometric capabilities over a wide range of applications. The key component is the well known camera lucida, an image-combining device that is used to superimpose the co...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/0165-0270(83)90048-1
更新日期：1983-05-01 00:00:00
abstract：:Neuroendocrine cells like chromaffin cells and PC-12 cells are established models for transport, docking and secretion of secretory vesicles. In micrographs, these vesicles are recognized by their electron dense core. The analysis of secretory vesicle distribution is usually performed manually, which is labour-intensi...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2008.05.022
更新日期：2008-08-15 00:00:00
abstract：BACKGROUND:Putative treatments derived from in vivo stem cell transplant-derived dopamine (DA) in hemiparkinsonian rats have been assessed via DA-agonist-induced rotations involving imbalanced intra-hemispheric striatal DA receptor stimulation. However, such tests obscure the natural responses of grafts to sensory stim...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2018.06.005
更新日期：2018-09-01 00:00:00
abstract：:MicroRNAs (miRNAs) finely tune messenger RNA (mRNA) expression. As the brain is a highly heterogeneous tissue, physiologically relevant miRNA expression profiling greatly benefits from sampling brain regions or nuclei. MiRNA expression profiling from individual samples is also important for investigating potential dif...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2012.05.033
更新日期：2012-07-30 00:00:00
abstract：BACKGROUND:Microelectrode arrays have been used successfully for neuronal stimulation both in vivo and in vitro. However, in most instances currents required to activate the neurons have been in un-physiological ranges resulting in neuronal damage and cell death. There is a need to develop electrodes which require less...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2015.03.024
更新日期：2015-06-15 00:00:00
abstract：INTRODUCTION:Transcranial magnetic stimulation (TMS) is used for assessing the excitability of cortical neurons and corticospinal pathways by determining the subject-specific motor threshold (MT). However, the MT is dependent on the TMS instrumentation and exhibits large variation. We hypothesized that between-subject ...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2011.10.004
更新日期：2012-01-30 00:00:00
abstract：:Although the classical Golgi method is a powerful means for structural analysis of the brain, it is generally considered to be an unpredictable technique making anatomists wary of using it. Often, even when successful staining has occurred, deposits of silver chromate crystals on the surface of the tissue obscure exam...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/0165-0270(95)00160-3
更新日期：1996-05-01 00:00:00
abstract：:This report describes a digitally controlled system for use in auditory physiology in which signal generation and data acquisition are under the control of a single microcomputer. The system is designed to generate the complex waveforms required in neuroethological studies, and is able to generate these signals at fre...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/0165-0270(91)90136-n
更新日期：1991-01-01 00:00:00
abstract：:Scanning ion conductance microscopy (SICM) takes advantage of the increase in the resistance which occurs if a glass microelectrode is closely approached to a poorly conducting membrane (Science 243 (1989) 641) and has been shown to be a promising technique to study membranes of living cells (Biophys J 73 (1997a) 653;...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/s0165-0270(02)00023-7
更新日期：2002-05-15 00:00:00
abstract：:A current source for neural stimulation is presented which converts arbitrary voltage signals to current-controlled signals while regulating the offset-voltage across the stimulation electrodes in order to keep the electrodes in an electrochemical state that allows for injecting a maximum charge. The offset-voltage ca...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2008.03.016
更新日期：2008-06-30 00:00:00
abstract：:According to the amyloid hypothesis, Abeta peptides are neurotoxic and underlie development and progression of Alzheimer's disease (AD). Multiple Abeta clearance mechanisms, including destruction of the peptides by proteolytic enzymes, are hypothesized to regulate physiological Abeta peptide levels. The insulin-degrad...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2008.11.010
更新日期：2009-03-30 00:00:00
abstract：:We investigated the usefulness of the Overall Rotarod Performance (ORP) test for evaluating overall locomotory ability in the 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP)-injected-mouse model of Parkinson's disease (PD). For this procedure, the mice are pretrained on the rotarod and then tested at a series of i...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/s0165-0270(98)00078-8
更新日期：1998-09-01 00:00:00
abstract：BACKGROUND:Polarized light imaging (PLI) has evolved into a powerful neuroimaging tool to analyze fiber tracts with submillimeter resolution in microtome sections of postmortem human brain tissue. In PLI polarized light changes its polarization state while passing through birefringent tissue, i.e., myelinated axons, wh...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2013.08.022
更新日期：2013-10-30 00:00:00
abstract：:Antibody microprobes are devices which have been used to determine the release sites of several neuropeptides. The production of microprobes using the previous methodology is a time-consuming procedure requiring a high level of skill. The aim of the study was to investigate alternative methods for the production of mi...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/0165-0270(94)90038-8
更新日期：1994-11-01 00:00:00
abstract：:Threshold depolarization is defined as the amplitude of the depolarization that will just bring a neurone to its firing threshold. Here, a novel technique is described for the measurement of threshold depolarization in resting human motoneurones. The tibial nerve was stimulated at an intensity that was just sufficient...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/0165-0270(91)90097-j
更新日期：1991-08-01 00:00:00
abstract：BACKGROUND:MRI-histology correlation studies of the ex vivo brain mostly employ fresh, extracted (ex situ) specimens, aldehyde fixed by immersion, which has several disadvantages for MRI scanning (e.g. deformation of the organ). A minority of studies are done ex vivo-in situ (unfixed brain), requiring an MRI scanner re...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2020.108903
更新日期：2020-11-01 00:00:00
abstract：:Technical challenges to record cortical unit spikes in conscious behavior monkeys have been presenting constraints in the investigation of cortical control of lower limb. Here we report a novel experimental system for investigation of correlation between cortical neuronal signals and standing and squatting movement. T...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2010.06.027
更新日期：2010-09-30 00:00:00
