Abstract:
:The authors describe the relation between clamping time and blood volume collected, and two enrichment systems of CD34+ stem cells from umbilical cord blood, to determine an excellent recovery with high proliferate ability and bone marrow reconstitution. After an obstetrician-based cord blood collection, the purification of stem cells was performed either with a combination of monoclonal antibodies (negative selections) using the Stem Sep method, or with a positive cells selection thanks to their surface CD34 antigens, using the Mini Macs system. An excellent recovery of hematopoietic progenitors, burst-forming unit erythroid, colony-forming unit granulocyte and macrophage, and colony-forming unit granulocyte, erythroid, monocyte, and macrophage, inversely related to the rising of clamping time, was performed with the Mini Macs system (54% of colonies, with a 90% purity), while with Stem Sep method, hematopoietic progenitor recovery was 35% (with an 80% purity). By applying early clamping of the umbilical cord blood a greater number of CD34+ cells was obtained and their clonogenic activity increased with enrichment. This is particularly useful, considering that the number of CD34+ stem cells contained in a unit of placental blood is enough for transplanting to a child, but not for an adult engraftment. Thus, using this method, a larger number of CD34+ stem cells can be obtained, which increases the possibility to reduce graft versus host disease also in adult patients, producing survival rates similar to the ones obtained with transplantation of bone marrow from unrelated donors.
journal_name
Pediatr Hematol Oncoljournal_title
Pediatric hematology and oncologyauthors
Pafumi C,Bosco P,Cavallaro A,Farina M,Leonardi I,Pernicone G,Bandiera S,Russo A,Giardina P,Chiarenza M,Calogero AEdoi
10.1080/08880010252899406subject
Has Abstractpub_date
2002-06-01 00:00:00pages
239-45issue
4eissn
0888-0018issn
1521-0669journal_volume
19pub_type
杂志文章abstract::Beta-thalassemia major is a severe, transfusion-dependent anemia that also causes infertility due to iron deposition to endocrine organs after overtransfusion. Very few pregnancies have been reported among such patients after modern therapies. In this study, 32 women with thalassemia who were admitted to Ali Asghar Ch...
journal_title:Pediatric hematology and oncology
pub_type: 杂志文章,多中心研究
doi:10.1080/08880010500313306
更新日期:2006-01-01 00:00:00
abstract::A high (18)F-fluorodeoxyglucose (FDG) uptake by positron emission tomography/computed tomography (PET/CT) imaging in sarcomas of adults has been reported. The current study aimed at defining the degree of (18)F-FDG uptake of pediatric sarcomas. This retrospective study included 29 patients (23 males, 6 females; mean a...
journal_title:Pediatric hematology and oncology
pub_type: 杂志文章
doi:10.3109/08880018.2011.602180
更新日期:2011-10-01 00:00:00
abstract::Adolescence is characterized by inter-related physiologic and emotional development. It is a particularly vulnerable period of life culminating into emergence as an adult. The majority of malignant bone tumors in pediatrics affect the adolescent patient. As a part of the treatment, amputation may be required for cure....
journal_title:Pediatric hematology and oncology
pub_type: 杂志文章
doi:10.1080/08880010490477374
更新日期:2004-09-01 00:00:00
abstract::The aim of the study is to evaluate the etiologic and clinical characteristics, treatment regimens, and outcome of the patients with intracranial tumors presenting with central diabetes insipidus (DI). Sixty-nine patients with intracranial tumors presenting with central DI between 1972 and 2012 were retrospectively ev...
journal_title:Pediatric hematology and oncology
pub_type: 杂志文章
doi:10.3109/08880018.2013.816984
更新日期:2013-10-01 00:00:00
abstract::Outlining the treatment for an unclassifiable lymphoid malignancy is often difficult. A highly undifferentiated lymphomatous mass that relapsed in spite of intense chemotherapy and autologous transplant is reported. At relapse, there was differentiation into myeloid lineage. Though remission was achieved with AML-type...
journal_title:Pediatric hematology and oncology
pub_type: 杂志文章
doi:10.1080/08880010902754883
更新日期:2009-03-01 00:00:00
abstract::To determine the effect of recombinant human granulocyte-colony stimulation factor (rhG-CSF) on the immune system, serum immunoglobulins, lymphocyte subsets, and serum cytokines were analyzed in eight pediatric patients with aplastic anemia (AA) during 8-week rhG-CSF therapy. The rhG-CSF was administered either subcut...
journal_title:Pediatric hematology and oncology
pub_type: 杂志文章
doi:10.3109/08880019409141675
更新日期:1994-05-01 00:00:00
abstract::A retrospective analysis was performed on febrile neutropenic episodes in patients with acute lymphoblastic leukemia (ALL) from 1992 to 2002. There were 222 febrile neutropenic episodes in 266 ALL patients with documented ANC < 500/mm(3). Of the 222 episodes, 98 (44%) had documented focus of infection; the rest were f...
journal_title:Pediatric hematology and oncology
pub_type: 杂志文章
doi:10.1080/08880010802106564
更新日期:2008-06-01 00:00:00
abstract::Ondansetron, a highly selective 5-HT3 receptor antagonist, is available in an intravenous (IV) formulation and tables, but syrup would be particularly useful in children. As chemotherapy can affect taste perceptions, this study was undertaken to determine the preference between two flavors of ondansetron syrup in chil...
journal_title:Pediatric hematology and oncology
pub_type: 临床试验,杂志文章,多中心研究,随机对照试验
doi:10.3109/08880019609030816
更新日期:1996-03-01 00:00:00
abstract::Li-Fraumeni syndrome (LFS) is one of the familial cancers characterized by different tumors and hereditary TP53 mutations. The adrenocortical carcinoma (ACC) association with acute leukemia is unusual in childhood, even in LFS. The authors here present a family with pR337P mutation in TP53 gene who had a child with ac...
journal_title:Pediatric hematology and oncology
pub_type: 杂志文章
doi:10.3109/08880011003663374
更新日期:2010-05-01 00:00:00
abstract::To investigate the possible clinical application of the hypothesis that insufficient induction of 3,4-dihydroxyphenylalanine decarboxylase (DDC) causes accumulation and secretion of 3,4-dihydroxyphenylalanine (DOPA) in unfavorable neuroblastomas, we measured plasma DOPA in 28 neuroblastoma patients. Abnormally high le...
journal_title:Pediatric hematology and oncology
pub_type: 杂志文章
doi:10.3109/08880019609033369
更新日期:1996-01-01 00:00:00
abstract::This study evaluated the efficacy of hydroxyurea treatment in the prevention of vaso-occlusive crises among children and teenagers with severe sickle cell anemia and sickle cell beta-thalassemia. Nineteen children and young adults with severe sickle cell disease were enrolled to the hydroxyurea treatment trial. The in...
journal_title:Pediatric hematology and oncology
pub_type: 临床试验,杂志文章
doi:10.1080/088800199277272
更新日期:1999-05-01 00:00:00
abstract::Modern treatment protocols lead to complete remission in a high proportion of patients with childhood acute lymphoblastic leukemia (ALL). However, a large number of them show a relapse of the disease. Treatment failure in these patients is mainly attributable to de novo or acquired resistance to a wide variety of cyto...
journal_title:Pediatric hematology and oncology
pub_type: 杂志文章
doi:10.1080/08880010600719303
更新日期:2006-09-01 00:00:00
abstract::The objective of this study was to ascertain the reactions and experiences of parents whose children were defined as false-positive cases in a research program of screening 6-month-old babies for neuroblastoma. Parents of seven of the eight infants falling into this category participated in the study. Parents of five ...
journal_title:Pediatric hematology and oncology
pub_type: 杂志文章
doi:10.3109/08880019409141651
更新日期:1994-03-01 00:00:00
abstract::A 12-year-old female is reported who presented with right hip pain for 6 months. With massive splenohepatomegaly and leukocytosis, CML was suspected and confirmed on bone-marrow examination and cytogenetics. Further investigations confirmed avascular necrosis (AVN) of the right femoral head. CML was treated by hydroxy...
journal_title:Pediatric hematology and oncology
pub_type: 杂志文章
doi:10.3109/08880018.2013.831961
更新日期:2014-09-01 00:00:00
abstract::The third International Conference on Immunotherapy in Pediatric Oncology was held in Frankfurt/Main, Germany, October 1-2, 2012. Major topics of the conference included (i) cellular therapies using antigen-specific and gene-modified T cells for targeting leukemia and pediatric solid tumors; (ii) overcoming hurdles an...
journal_title:Pediatric hematology and oncology
pub_type: 杂志文章
doi:10.3109/08880018.2013.802106
更新日期:2013-08-01 00:00:00
abstract::Immune thrombocytopenia (ITP) is a frequently encountered disease in childhood. Recent reports pointed to the benefit of high-dose steroid in ITP treatment since it resulted in a better outcome in a shorter time than IV immunoglobulin therapy. In the authors' clinic, mainly after 1984, megadose methyl prednisolone (MD...
journal_title:Pediatric hematology and oncology
pub_type: 临床试验,杂志文章
doi:10.1080/08880010590964291
更新日期:2005-07-01 00:00:00
abstract::To evaluate the quantitative aspects of the shift in production from fetal hemoglobin (HbF) to adult hemoglobin (HbA), the HbF and HbA mass were estimated in a preterm infant (gestational age 29 weeks) for 22 weeks after an exchange transfusion the second day of life, leading to an initial HbA% of 100. Up until the es...
journal_title:Pediatric hematology and oncology
pub_type: 杂志文章
doi:10.3109/08880019809016572
更新日期:1998-09-01 00:00:00
abstract::It is well known in the literature that cancer creates educationally related barriers for all children, which may or may not contribute to clinically relevant school problems. The goal of this study was to obtain a base rate for the characteristic pattern of school functioning for children with leukemia, so that the c...
journal_title:Pediatric hematology and oncology
pub_type: 杂志文章
doi:10.3109/08880019709030898
更新日期:1997-03-01 00:00:00
abstract::The FLT3 gene encodes a tyrosine kinase receptor that regulates proliferation and differentiation of hematopoietic cells. Recently, the internal duplication of FLT3 has been observed in hematological malignancies, suggesting the involvement of these mutations in leukemogenesis. The authors analyzed the expression of F...
journal_title:Pediatric hematology and oncology
pub_type: 杂志文章
doi:10.1080/088800199276994
更新日期:1999-09-01 00:00:00
abstract::Infections remain a serious complication in pediatric oncology patients. To determine if daily bathing with Chlorhexidine gluconate can decrease the rate of nosocomial infection in pediatric oncology patients, we reviewed rates of infections in pediatric oncology patients over a 14-month span. Intervention group recei...
journal_title:Pediatric hematology and oncology
pub_type: 杂志文章
doi:10.3109/08880018.2015.1013588
更新日期:2015-01-01 00:00:00
abstract::A surveillance study was performed on the pattern and antimicrobial susceptibilities of microorganisms isolated from blood cultures in the main pediatric oncology unit in the United Arab Emirates. The only previous data were published in 1995. During 1998-2000, the pattern of bloodstream infection changed. Enterobacte...
journal_title:Pediatric hematology and oncology
pub_type: 杂志文章
doi:
更新日期:2003-06-01 00:00:00
abstract::Acute basophilic leukemia is a distinct entity of Acute Myeloid Leukemia (AML) with primary differentiation to basophils. Increased basophil count has been described in AML cases with translocation t(6;9)(p23;q34) or other chromosomal abnormalities. We describe a 15-year old female teenager with AML and excess periphe...
journal_title:Pediatric hematology and oncology
pub_type: 杂志文章
doi:10.3109/08880018.2014.883655
更新日期:2014-03-01 00:00:00
abstract::Neuroblastoma presenting with obstructive jaundice is a rare event. Management of this condition includes surgery, chemotherapy, radiotherapy, temporary cholecystostomy tube, endoscopic retrograde cholangiopancreatography (ERCP), and internal biliary drainage (IBD). We herein describe our experience with one infant af...
journal_title:Pediatric hematology and oncology
pub_type: 杂志文章
doi:10.3109/08880018.2014.981901
更新日期:2015-04-01 00:00:00
abstract::An 11-year-old girl with hereditary factor-VII deficiency and her family have been studied for factor VII coagulation activity (VII:C) and factor VII antigen level (VII:Ag). The proband had 11% VII:C, whereas her VII:Ag was at a level that corresponds to about 50% coagulation activity. Forty-two members of the proband...
journal_title:Pediatric hematology and oncology
pub_type: 杂志文章
doi:10.3109/08880018709141261
更新日期:1987-01-01 00:00:00
abstract::Primary CNS germ cell tumors (GCT) arise in the suprasellar and pineal regions. Suprasellar GCT may remain radiographically occult during the early symptomatic period. Although theoretically possible, it is more difficult to identify presymptomatic disease in the pineal region. Given the sensitivity of GCT to cytotoxi...
journal_title:Pediatric hematology and oncology
pub_type: 杂志文章
doi:10.1080/08880010701665777
更新日期:2008-01-01 00:00:00
abstract::Disaccharidases activity in the intestinal mucosa samples of rats was examined after intragastric and intramuscular methotrexate therapy. Methotrexate was given on a twice a week schedule (1 mg/kg body weight). The animals were sacrificed after 2 weeks, 1 month, and 2 months of this therapy. A statistically significan...
journal_title:Pediatric hematology and oncology
pub_type: 杂志文章
doi:10.3109/08880019009033397
更新日期:1990-01-01 00:00:00
abstract::This study evaluated the effects of urokinase in the prevention of central venous catheter (CVC)-related complications in children with malignancy. Fifteen patients with 16 CVCs (study group A) received an intraluminal application of urokinase (10,000 IU in each catheter lumen for 4 h) once a week. They were monitored...
journal_title:Pediatric hematology and oncology
pub_type: 临床试验,杂志文章
doi:10.1080/088800102753541323
更新日期:2002-04-01 00:00:00
abstract::In 33 children, 23 with acute lymphoblastic leukemia (ALL) and 10 with solid tumors, the phenotype of the enzyme adenosine deaminase (ADA) was detected in the erythrocytes by electrophoresis in cellulose acetate. In all children the ADA enzyme activity was also determined in the plasma by spectrophotometry at the onse...
journal_title:Pediatric hematology and oncology
pub_type: 杂志文章
doi:10.3109/08880019609030840
更新日期:1996-07-01 00:00:00
abstract::Thrombo-embolism in childhood is a multifactorial disorder. The present study is a case-control study that investigated the role of genetic and acquired risk factors in 60 children with thrombosis and compared the results with the controls. Acquired and inherited risk factors precipitating thrombosis were present in 7...
journal_title:Pediatric hematology and oncology
pub_type: 杂志文章
doi:10.1080/08880010600646324
更新日期:2006-07-01 00:00:00
abstract::This study examined both the endogenous and exogenous (therapy-related) pharmacokinetics of the cytokine granulocyte-macrophage colony-stimulating factor (GM-CSF) in neutropenic children with solid and systemic malignancies. The daily endogenous GM-CSF serum concentration before application was 29 pg/mL. During the 10...
journal_title:Pediatric hematology and oncology
pub_type: 杂志文章
doi:10.1080/088800199276796
更新日期:1999-11-01 00:00:00