Abstract：
BACKGROUND:Viscum album agglutinin-1 (VAA-1) is assumed to be the biologically most active ingredient of misteltoe extracts that are often used as adjuvant cancer therapy. To develop new approaches for lung cancer treatment, we evaluated the antineoplastic activity of VAA-1 alone and in combination with other chemotherapeutic drugs, including doxorubicin, cisplatin and taxol in the human lung carcinoma cell line A549. MATERIALS AND METHODS:Cytotoxicity was determined by 5-bromo-2'-deoxyuridine (BrdU) ELISA-assays and drug interaction assessed by the isobologram method. Analysis of cell cycle distribution was obtained using flow cytometry. RESULTS:For all drug combinations tested the outcome was additive with the combination of VAA-1 and cycloheximide showing strong synergistic effects. Moreover, VAA-1 induced G1-phase accumulation mechanisms without causing apoptosis. CONCLUSION:Our findings suggest that the simultaneous administration of VAA-1 with all anticancer agents tested is advantageous since cytotoxic effects are enhanced. These data may provide new clinicalperspectives in future mistletoe therapy.
journal_name
Anticancer Resjournal_title
Anticancer researchauthors
Siegle I,Fritz P,McClellan M,Gutzeit S,Mürdter TEsubject
Has Abstractpub_date
2001-07-01 00:00:00pages
2687-91issue
4Aeissn
0250-7005issn
1791-7530journal_volume
21pub_type
杂志文章abstract：BACKGROUND:Clofarabine is a second-generation nucleoside analogue. The aim of the study was the analysis of ex vivo activity of clofarabine and 14 other anticancer drugs in pediatric and adult acute lymphoblastic (ALL) and myeloid (AML) leukemia. PATIENTS AND METHODS:The ex vivo drug resistance profile was analyzed in...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2009-05-01 00:00:00
abstract：BACKGROUND:Doxorubicin remains one of the few drugs with consistent single agent activity in advanced Soft Tissue Sarcomas (STS), with a demonstrated dose-response relationship. Liposomal-encapsulated Doxorubicin (LED) has been shown to be at least as active as free doxorubicin in experimental models, and phase I and I...
journal_title：Anticancer research
pub_type： 临床试验,杂志文章
doi：
更新日期：2000-01-01 00:00:00
abstract：BACKGROUND:Medullary thyroid carcinoma (MTC) is a calcitonin-producing tumor of the parafollicular C-cells, accounting for 5-10% of all thyroid tumors. To date, the only effective treatment is the early and total surgical removal of all neoplastic tissue. As the prognosis of patients with advanced MTC, unresectable or ...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2005-11-01 00:00:00
abstract：BACKGROUND:HER-2/neu onco-protein is increasingly being recognized as a target for therapy in solid tumors, but data on its role in malignant melanoma is currently limited. Our study objective was to determine a relationship, if any, between HER-2/neu overexpression and malignant melanoma. PATIENTS AND METHODS:Overexp...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2003-09-01 00:00:00
abstract：BACKGROUND/AIM:Anaplastic thyroid cancer (ATC) is one of the most aggressive human malignancies, remaining generally incurable. Histone deacetylase (HDAC) seems to play a role in regulating transcription of genes involved in ATC, making HDAC inhibitors (HDACI) promising anticancer drugs for ATC. The purpose of this rev...
journal_title：Anticancer research
pub_type： 杂志文章,评审
doi：10.21873/anticanres.13220
更新日期：2019-03-01 00:00:00
abstract：BACKGROUND:The differential diagnosis of well-differentiated tumors of follicular cell origin remains a most problematic task in thyroid pathology. Therefore, various diagnostic/prognostic thyroid markers are currently being studied. The aim of our study was to evaluate the proliferative MIB-1 and topoisomerase II-alph...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2005-05-01 00:00:00
abstract：AIM:This study assessed the symptoms and health-related quality of life (HRQOL) of patients with advanced non-small cell lung cancer (NSCLC) and examined the symptom-associated characteristics. PATIENTS AND METHODS:The symptoms of 122 patients with NSCLC scheduled for chemotherapy before starting treatment were survey...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2016-08-01 00:00:00
abstract：:Ten patients with neuroblastoma were treated with a standard pulsed administration schedule of cis-platinum and etoposide (VP16-213). Two patients are experiencing good partial responses lasting over 8 months. One other patient had a partial response. Toxicity was mild and limited to high frequency hearing loss, tempo...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：1986-11-01 00:00:00
abstract：AIM:To investigate the possibility of enhancing an anti-metastatic effect of 5-fluorouracil (5-FU) on colorectal cancer (CRC) cells by combining it with continuous calcium supplementation. MATERIALS AND METHODS:Optimal doses of 5-FU with/without lactate salt (CaLa) were determined via clonogenicity and 3-(4,5-dimethyl...
journal_title：Anticancer research
pub_type： 杂志文章
doi：10.21873/anticanres.11295
更新日期：2017-01-01 00:00:00
abstract：BACKGROUND:The upper outer quadrant (UOQ) of the breast is the most frequent site for incidence of breast cancer, but the reported disproportionate incidence in this quadrant appears to rise with year of publication. MATERIALS AND METHODS:In order to determine whether this increasing incidence in the UOQ is an artifac...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2005-05-01 00:00:00
abstract：BACKGROUND/AIM:The clinical significance of surgery for secondary small intestinal non-Hodgkin's lymphomas (NHL) remains unknown. This study aimed to investigate the efficacy of resection for both primary and secondary small intestinal NHL. PATIENTS AND METHODS:Twenty patients with small intestinal lymphoma who underw...
journal_title：Anticancer research
pub_type： 杂志文章
doi：10.21873/anticanres.14206
更新日期：2020-04-01 00:00:00
abstract：:In order to improve the knowledge of prolactin receptors (PRL-R) in human breast tumors, we studied PRL-R modulation by lactogenic and steroid hormones in the PRL-R rich human breast cancer cell-line, T-47 D. The PRL-R were assayed on a preparation of cell total membranes. We demonstrated an abnormal homologous in vit...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：1989-05-01 00:00:00
abstract：BACKGROUND:Thymidine phosphorylase (dThdPase) is identical to platelet-derived endothelial cell growth factor (PD-ECCF) and has a function of angiogenesis in vitro and in several types of human carcinoma tissues. We have reported that expression of dThdPase was an independent prognostic factor in 116 gastric carcinomas...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2003-11-01 00:00:00
abstract：BACKGROUND/AIM:Primary brain tumors are unique tumors due to their different pathobiological behavior, while they rarely metastasize outside the central nervous system. Regarding the oncogenesis of primary brain tumors, it was shown that changes in functions of p16 and mouse double minute 2 homolog (MDM2) are related t...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2015-07-01 00:00:00
abstract：BACKGROUND/AIM:The aim of the study was to evaluate the necessity of gastrectomy including endoscopic submucosal dissection (ESD) scar with margin-negative early gastric cancer (EGC). PATIENTS AND METHODS:We analyzed 83 patients with EGC who received additional gastrectomy after non-curative ESD and evaluated the risk...
journal_title：Anticancer research
pub_type： 杂志文章
doi：10.21873/anticanres.12855
更新日期：2018-09-01 00:00:00
abstract：:An immunohistochemical study of 155 squamous cell carcinomas of the oesophagus, squamous epithelium adjacent to the tumour (n = 80), dysplastic epithelium (n = 16) and controls (n = 23) indicates that Ki67 and p53 expression is frequently present in premalignant oesophageal lesions and their related squamous cell carc...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：1994-01-01 00:00:00
abstract：:Along with advancements in treatment, early detection of primary tumor, and relapse seems to remain a key factor for improving the survival rate of patients with head and neck squamous cell carcinoma (HNSCC), in which a high proportion of patients are diagnosed at an advanced stage. Recent advancements in basic resear...
journal_title：Anticancer research
pub_type： 杂志文章,评审
doi：
更新日期：2011-04-01 00:00:00
abstract：:The bcl-2 proto-oncogene suppresses apoptosis in a variety of cell types and is essential for normal renal development. PAX8 is a member of the paired box class of transcription factors and is developmentally regulated. bcl-2 and PAX8 are both expressed in the kidney during development and throughout life, demonstrati...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：1997-09-01 00:00:00
abstract：UNLABELLED:Magnetic drug targeting (MDT) is a new locoregional chemotherapy method that increases the drug dose in the tumour region, while simultaneously reducing the overall dose through the application of chemotherapeutic-bound superparamagnetic nanoparticles, which are focused by an external magnetic field to the d...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2010-05-01 00:00:00
abstract：BACKGROUND:This was a validation study of a regional register of oral cancer in Örebro, Sweden. The purpose was to assess the rate of errors in baseline, and treatment, and the completeness and accuracy of data on recurrences. MATERIALS AND METHODS:A total of 653 cases with squamous cell cancer in the oral cavity were...
journal_title：Anticancer research
pub_type： 杂志文章
doi：10.21873/anticanres.13109
更新日期：2019-01-01 00:00:00
abstract：:A new cell line, CC3/CUHK3, was established from a squamous carcinoma of the uterine cervix from a Chinese patient. Light microscopy of the cell line revealed keratin and electron microscopy demonstrated tonofilaments and desmosomes, thereby confirming its epithelial nature. The population doubling time of CC3/CUHK3, ...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：1990-01-01 00:00:00
abstract：:The proliferative rate as well as the activity of 3-hydroxy-3-methylglutaryl CoA (HMG CoA) reductase, which regulates de novo synthesis of mevalonate, was comparable in the two human breast cancer cell lines Hs578T and MDA-231 when cultured in the presence of serum. Upon treatment with mevinolin (an HMG CoA reductase ...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：1992-03-01 00:00:00
abstract：BACKGROUND:The proportion of geriatric gastric cancer (GC) patients with comorbidities has been increasing in Japan. This study was designed to evaluate the prognostic influence of the extent of lymph node dissection (END) and perioperative morbidities in patients with GC. PATIENTS AND METHODS:Between 2008 and 2013, t...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2016-04-01 00:00:00
abstract：:The high glucose consumption of tumour cells has been well known since Warburg's investigations. Nevertheless, the reason for this phenomenon is still a matter of speculation. Glucose uptake of tumour cells is mainly mediated by GLUT1. In this study, the question of whether glucose uptake as well as expression of GLUT...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2005-09-01 00:00:00
abstract：:Three histamine H2-receptor antagonists, Cimetidine, Metiamide and Ranitidine, were tested for their inhibitory effect on two experimental bowel cancer models. In the first model mitotic rates were measured in dimethylhydrazine-induced tumors of rat colon and in the second model volumetric changes in human large bowel...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：1983-01-01 00:00:00
abstract：BACKGROUND:The size of colorectal polyps is important in the clinical management of these lesions. When using a conventional ruler (the tool of pathologists worldwide), we have previously found unacceptably high intra- and inter-observer variations in assessing the size of phantom polyps. The aim of this study was to a...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2008-05-01 00:00:00
abstract：BACKGROUND:The aim of this study was to assess the metallothionein (MT), maintenance protein 2 (Mcm-2) and Ki-67 expressions in adrenocortical adenomas and carcinomas in comparison to normal tissue and evaluate the correlations between these markers of proliferation and between these markers and tumor diameter. MATERI...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2008-09-01 00:00:00
abstract：BACKGROUND:Locally recurrent rate of advanced head and neck squamous cell carcinoma (HNSCC) still remains high and the treatment is controversial. PATIENTS AND METHODS:We retrospectively analyzed ninety-three patients with recurrent advanced oral cancer from 2009 to 2013. Sixty-four patients are in the docetaxel with ...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2014-07-01 00:00:00
abstract：:Small cell carcinomas of the gastrointestinal tract are rare and clinically aggressive tumors. A case is presented of a 70 year-old woman who presented with small bowel obstruction and was found to have a cecal mass. She underwent right hemicolectomy, and histopathology showed a small cell carcinoma arising in the bac...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2013-04-01 00:00:00
abstract：BACKGROUND:The association of TN expression, tumour grading and expression of commonly used histopathologic prognostic factors (p53 and estrogen receptor) was examined in 62 cases of invasive ductal carcinoma of the breast. MATERIAL AND METHODS:Following histological grading immunohistochemical reactions were undertak...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：1999-01-01 00:00:00
