Abstract:
:Immunological mechanisms are thought to play an important role in the pathogenesis of some cochleo-vestibular diseases. This study attempts to present further evidence of autoantibodies reactive against guinea pig inner ear proteins found in patients with autoimmune inner ear diseases (AIED) and specifically identifies the main target antigens of these antibodies. Sera from 110 patients with a clinical diagnosis of either rapidly progressive sensorineural hearing loss (n = 32). Ménière's disease (n = 41), sudden deafness (n = 6) or other aetiologies of hearing loss (n = 11) were screened by the Western blot technique. Forty-four percent of the patients' sera had antibodies to several inner ear proteins, of which the 30, 42 and 68 kDa proteins were found to be the most reactive. These highly reactive proteins were identified by gas-phase micro sequencing after digestion with trypsin and separation of peptide fragments by high-performance liquid chromatography. A partial sequence of each protein was determined. These data, together with those obtained from 2-dimensional gel electrophoresis followed by Western blotting, demonstrated that the 30 and 42 kDa inner ear proteins are the major peripheral myelin protein P0 and the beta-actin protein, respectively, while sequence analysis indicated that the 68 kDa protein is novel. These findings further support the hypothesis that several populations of antibodies may contribute to the enhanced immunological activity of AIED patients. They also add a new dimension to our knowledge of AIED and may open new avenues in the development of simple serological assays, which are easier to perform and more rapid than Western blotting.
journal_name
Acta Otolaryngoljournal_title
Acta oto-laryngologicaauthors
Boulassel MR,Deggouj N,Tomasi JP,Gersdorff Mdoi
10.1080/000164801300006236subject
Has Abstractpub_date
2001-01-01 00:00:00pages
28-34issue
1eissn
0001-6489issn
1651-2251journal_volume
121pub_type
杂志文章abstract::Nitric oxide synthase (NOS) isoforms I and III were localized in the guinea pig cochlea by indirect immunohistochemistry using frozen sections and paraffin sections. NOS I staining was observed in the cytoplasm of outer hair cells, in nerve cell somata and fibers of the spiral ganglion, and in axonal profiles of the s...
journal_title:Acta oto-laryngologica
pub_type: 杂志文章
doi:10.3109/00016489609137914
更新日期:1996-09-01 00:00:00
abstract::In a previous study (1) it was found that in healthy subjects Ocular (Counter) Rotation is mainly due to otolith stimulation and only to a minor extent induced by slanted visual structures. Stimulation of the neck by tilting the trunk laterally upwards did not result in a systematic rotation of the eyes. In the presen...
journal_title:Acta oto-laryngologica
pub_type: 杂志文章
doi:10.3109/00016489109138369
更新日期:1991-01-01 00:00:00
abstract::Endoscopy is needed for reliable evaluation of the treatment of nasal polyposis. In this study, we compared the reproducibility of various score systems for staging nasal polyposis and the inter-individual variations between investigators. The mass of the polyps was assessed by five methods, three new techniques (numb...
journal_title:Acta oto-laryngologica
pub_type: 杂志文章
doi:10.1080/000164800760370873
更新日期:2000-01-01 00:00:00
abstract:CONCLUSIONS:The expression of phosphorylcholine (ChoP) in the surface of nontypeable Haemophilus influenzae (NTHi) is an important factor in the pathogenesis of persistent otitis media with effusion (OME) in humans. OBJECTIVES:Lipooligosaccharide (LOS) in NTHi undergoes phase variation in expression of the ChoP epitop...
journal_title:Acta oto-laryngologica
pub_type: 杂志文章
doi:10.1080/00016480802468195
更新日期:2009-08-01 00:00:00
abstract:CONCLUSIONS:There was a moderate chance of cure after surgical salvage of recurrent hypopharyngeal squamous cell carcinoma (SCC). However, surgical salvage was only feasible for early recurrent tumor. Close follow-up surveillance to detect early recurrence is essential after primary treatment of patients. OBJECTIVES:D...
journal_title:Acta oto-laryngologica
pub_type: 杂志文章
doi:10.3109/00016489.2013.815365
更新日期:2013-10-01 00:00:00
abstract:CONCLUSION:The use of both gadolinium chloride (GdCl(3)) and osmium tetroxide (OsO(4)) allowed for the visualization of the membranous labyrinth and other intralabyrinthine structures, at different intensities, as compared with the control sample. This initial comparison shows the advantages of GdCl(3) in radiological ...
journal_title:Acta oto-laryngologica
pub_type: 杂志文章
doi:10.3109/00016489.2012.715751
更新日期:2013-01-01 00:00:00
abstract:OBJECTIVE:To investigate pathological gait in patients with unilateral acoustic neuroma using tactile sensors placed under both feet. MATERIAL AND METHODS:Forty-three patients were enrolled in the study. They were categorized into two groups: the small tumor group had tumors < 2 cm from the porus acousticus without an...
journal_title:Acta oto-laryngologica
pub_type: 杂志文章
doi:10.1080/00016480310000566
更新日期:2004-05-01 00:00:00
abstract::Background: Chronic exposure to whole body vibration can affect the gastrointestinal system, the lumbar spine, the peripheral veins and the vestibulocochlear system. Aims/Objectives: The aim of this study was to evaluate the effects of vibration on the vestibular system in noisy and noise-free environments using the v...
journal_title:Acta oto-laryngologica
pub_type: 杂志文章
doi:10.1080/00016489.2019.1666213
更新日期:2019-11-01 00:00:00
abstract::Background: The various pathological types of non-squamous cell carcinomas (nSCCs) of the larynx accounted for about 5% laryngeal malignancies, but the knowledge regarding these pathological behaviors, therapeutic models and prognostic factors was limited.Objectives: To investigate the clinical characteristics and the...
journal_title:Acta oto-laryngologica
pub_type: 杂志文章
doi:10.1080/00016489.2019.1700305
更新日期:2020-02-01 00:00:00
abstract::In the guinea pig, the organ of Corti and the spiral ganglion cells were destroyed by administering gentamicin into the inner ear. The antero-ventral cochlear nucleus (AVCN) was studied with electron microscopy 15 and 30 days after treatment. There does not seem to be a specific type of degeneration induced by the dru...
journal_title:Acta oto-laryngologica
pub_type: 杂志文章
doi:10.3109/00016488609108643
更新日期:1986-07-01 00:00:00
abstract::A prospective study was carried out to assess the possible biochemical background of the AAm production by HVF in accordance with selected histological types and voice disorders. The mass of new tissue forming HVF in different clinical cases led us to suspect, according to our previous results, that PGs, LTs and other...
journal_title:Acta oto-laryngologica
pub_type: 杂志文章
doi:10.3109/00016489309135840
更新日期:1993-05-01 00:00:00
abstract:CONCLUSION:The reduced caliber of the anterior inferior cerebellar artery (AICA) on the affected side in patients with sudden unilateral profound hearing loss (SUPHL) may reflect a perfusion deficit which, combined to a lesser degree with anatomic abnormalities in the vertebrobasilar system, would be implicated in the ...
journal_title:Acta oto-laryngologica
pub_type: 杂志文章
doi:10.1080/00016480510029275
更新日期:2005-08-01 00:00:00
abstract::Glyconjugate expression in formalin-fixed, paraffin-embedded Sjögren's syndrome labial glands (nine cases) was compared with that in normal glands (12 samples) using a wide panel of lectins. Mucous cells expressed mainly mucous-type glycoproteins including sialyl, fucosyl, galactosyl and galactosaminosyl residues, and...
journal_title:Acta oto-laryngologica
pub_type: 杂志文章
doi:10.3109/00016489709114217
更新日期:1997-11-01 00:00:00
abstract:PURPOSE:To analyze the outcomes following re-irradiation for local recurrence of rare head and neck tumors. MATERIAL AND METHODS:We retrospectively analyzed 11 patients who had received intensity-modulated radiation therapy (IMRT) for recurrent tumors in the head and neck except for laryngopharynx. RESULTS:Primary tu...
journal_title:Acta oto-laryngologica
pub_type: 杂志文章
doi:10.1080/00016489.2018.1438662
更新日期:2018-08-01 00:00:00
abstract:BACKGROUND:Li quick repositioning maneuver for treatment of geotropic horizontal canal benign paroxysmal positional vertigo (HC-BPPV) was reported in the literature. OBJECTIVE:The aim of this study is to observe the clinical efficacy of the Li quick repositioning maneuver for the treatment of geotropic HC-BPPV. METHO...
journal_title:Acta oto-laryngologica
pub_type: 杂志文章,随机对照试验
doi:10.1080/00016489.2018.1476778
更新日期:2018-09-01 00:00:00
abstract:OBJECTIVE:We used ocular vestibular evoked myogenic potentials to investigate the relationship between residual dizziness and utricular function following the canalith repositioning procedure for benign paroxysmal positional vertigo. METHODS:Ocular vestibular evoked myogenic potentials were measured in 44 patients (40...
journal_title:Acta oto-laryngologica
pub_type: 杂志文章
doi:10.1080/00016489.2017.1347824
更新日期:2017-11-01 00:00:00
abstract::Based on the experimental results of thyroplasty, thyroplasty type I which aims at medical shifting the vocal cord was performed on 8 patients with dysphonia, 6 with vocal cord paralysis and 2 with vocal cord atrophy. The surgery was conducted on either in- or out-patient basis and local anesthesia was used. Usually, ...
journal_title:Acta oto-laryngologica
pub_type: 杂志文章
doi:10.3109/00016487509121353
更新日期:1975-11-01 00:00:00
abstract::The objective of this study was to determine whether methylcobalamin inhibits the ototoxic action of gentamicin. Fourteen pigmented guinea pigs were randomly divided into experimental and control groups. Each animal in the experimental group was given a large single dose of gentamicin (125 mg/kg i.m.), followed by imm...
journal_title:Acta oto-laryngologica
pub_type: 杂志文章
doi:10.1080/000164801300102752
更新日期:2001-04-01 00:00:00
abstract::As part of an early intervention study on the acceptability of auditory rehabilitative intervention, we examined patient complaints and clinical findings of balance disorders. Hallam et al. have discussed desynchrony between such findings in hospital patient populations, and the aim of the present study was to investi...
journal_title:Acta oto-laryngologica
pub_type: 杂志文章
doi:10.3109/00016489109137373
更新日期:1991-01-01 00:00:00
abstract:CONCLUSIONS:Protocol limitations were identified and accounted for in the analysis. Percent gradient equilibrated (PGE) was affected by driving gradient direction in a similar manner to other efficiency measures. A finer resolution of possible age-related changes in eustachian tube opening efficiency is expected with t...
journal_title:Acta oto-laryngologica
pub_type: 杂志文章
doi:10.3109/00016489.2014.882017
更新日期:2014-06-01 00:00:00
abstract:CONCLUSIONS:Endoscopic laser microsurgery (ELM) provides comparable oncological outcomes, and improved functional recovery and laryngeal preservation compared with open partial laryngopharyngectomy (OPLP) in patients with early squamous cell carcinoma of the hypopharynx (HPSCC). The use of open surgery is declining. Th...
journal_title:Acta oto-laryngologica
pub_type: 杂志文章
doi:10.3109/00016489.2013.805341
更新日期:2013-10-01 00:00:00
abstract::The ultrastructure of the geniculate ganglion was studied in the guinea pig and monkey. Two cytologically distinct colonies of ganglion cells were seen and identified as light and dark cells. All neurons were unmyelinated and most were unipolar, although a few bipolar cells were seen. The axons, upon leaving the cell ...
journal_title:Acta oto-laryngologica
pub_type: 杂志文章
doi:10.3109/00016488209130869
更新日期:1982-01-01 00:00:00
abstract:CONCLUSION:Chances of hearing preservation are better in patients with vestibular schwannoma (VS) from the superior vestibular nerve (SVN) than from the inferior vestibular nerve (IVN). Pathologic posturography and electronystagmography (ENG) findings are more common in schwannomas from the SVN compared with tumors fro...
journal_title:Acta oto-laryngologica
pub_type: 杂志文章
doi:10.3109/00016489.2010.536991
更新日期:2011-05-01 00:00:00
abstract::Unilateral tubal occlusion was performed in 10 cats, which were sacrificed 26-44 months later. During the observation period repeated bilateral tympanometry and auditory nerve response were recorded. Paracentesis with evacuation of effusion was performed up to eight times and the effusion was examined by viscometry. A...
journal_title:Acta oto-laryngologica
pub_type: 杂志文章
doi:10.3109/00016488409132936
更新日期:1984-05-01 00:00:00
abstract::Overexpression of epidermal growth factor receptor (EGF-R) is a common characteristic of epidermoid tumors and its expression in head and neck squamous cell carcinoma biopsies and cell lines has been reported previously by several authors. With the aim to provide more structural and functional details about the protei...
journal_title:Acta oto-laryngologica
pub_type: 杂志文章
doi:10.3109/00016489309135902
更新日期:1993-11-01 00:00:00
abstract::Evaluation of some 200 endolymph proteins indicates that they are predominantly derived from plasma. However, the profile of endolymph proteins is remarkably similar to that of perilymph and entirely different from that of plasma. This supports the current consensus that perilymph rather than plasma is the (direct) so...
journal_title:Acta oto-laryngologica
pub_type: 杂志文章,评审
doi:10.1080/00016489950181260
更新日期:1999-01-01 00:00:00
abstract::The embryonic development of the crista ampullaris of the CBA/CBA mouse was followed both in organ culture of explanted inner ears of the 16th gestational day and in vivo from the 16th gestational day until the 21st day, an age corresponding to birth. Cytodifferentiation of the sensory epithelium of the crista ampulla...
journal_title:Acta oto-laryngologica
pub_type: 杂志文章
doi:10.3109/00016488309132868
更新日期:1983-07-01 00:00:00
abstract:CONCLUSION:Endoscopic cauterization of the sphenopalatine artery and anterior ethmoid artery is a first-line standard of care in managing intractable epistaxis, after the failure of previous packing. Epistaxis occurs in 12% of the population. Treatment is often based on nasal packing that could be poorly effective in t...
journal_title:Acta oto-laryngologica
pub_type: 杂志文章,多中心研究
doi:10.3109/00016481003621538
更新日期:2010-09-01 00:00:00
abstract:CONCLUSION:Salivary gland diseases should be handled with a high index of suspicion. Although an inference could be drawn it is not clear from this study if primary head and neck cancers (HNCs) have a significant association with HIV/AIDS. OBJECTIVE:HIV/AIDS manifestations and malignancies do occur in the head and nec...
journal_title:Acta oto-laryngologica
pub_type: 杂志文章
doi:10.1080/00016480701230936
更新日期:2007-11-01 00:00:00
abstract:CONCLUSION:Lactobacillus rhamnosus GG (L.GG) was present in all adenoids of children receiving the L. GG product. However, since L.GG was also found from the placebo group, one cannot confirm its effect on the occurrence of rhinovirus (RV) or enterovirus (EV). OBJECTIVES:The present study was conducted to determine wh...
journal_title:Acta oto-laryngologica
pub_type: 杂志文章,随机对照试验
doi:10.3109/00016489.2015.1027412
更新日期:2015-08-01 00:00:00